Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05070 and PGA1_c05080 overlap by 1 nucleotides PGA1_c05080 and PGA1_c05090 are separated by 425 nucleotides PGA1_c05090 and PGA1_c05100 are separated by 27 nucleotides PGA1_c05100 and PGA1_c05110 are separated by 161 nucleotides
PGA1_c05070: PGA1_c05070 - putative pyridoxal-phosphate-dependent aminotransferase, at 499,811 to 500,908
_c05070
PGA1_c05080: PGA1_c05080 - WxcM-like, C-terminal., at 500,908 to 501,342
_c05080
PGA1_c05090: PGA1_c05090 - putative glycosyl transferase, at 501,768 to 502,712
_c05090
PGA1_c05100: PGA1_c05100 - transcriptional regulator, GntR type, at 502,740 to 503,402
_c05100
PGA1_c05110: PGA1_c05110 - dihydrodipicolinate synthase domain-containing protein, at 503,564 to 504,523
_c05110
Position (kb)
501
502
503 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 500.787 kb on + strand, within PGA1_c05070 at 500.797 kb on + strand, within PGA1_c05070 at 500.797 kb on + strand, within PGA1_c05070 at 500.797 kb on + strand, within PGA1_c05070 at 500.805 kb on - strand at 500.813 kb on + strand at 500.831 kb on - strand at 500.831 kb on - strand at 500.834 kb on + strand at 500.880 kb on + strand at 500.888 kb on - strand at 500.910 kb on + strand at 500.958 kb on - strand, within PGA1_c05080 at 500.958 kb on - strand, within PGA1_c05080 at 500.973 kb on - strand, within PGA1_c05080 at 500.980 kb on + strand, within PGA1_c05080 at 500.981 kb on + strand, within PGA1_c05080 at 500.989 kb on + strand, within PGA1_c05080 at 500.995 kb on - strand, within PGA1_c05080 at 501.095 kb on + strand, within PGA1_c05080 at 501.100 kb on + strand, within PGA1_c05080 at 501.100 kb on + strand, within PGA1_c05080 at 501.111 kb on + strand, within PGA1_c05080 at 501.111 kb on + strand, within PGA1_c05080 at 501.111 kb on + strand, within PGA1_c05080 at 501.111 kb on + strand, within PGA1_c05080 at 501.111 kb on + strand, within PGA1_c05080 at 501.111 kb on + strand, within PGA1_c05080 at 501.111 kb on + strand, within PGA1_c05080 at 501.111 kb on + strand, within PGA1_c05080 at 501.119 kb on - strand, within PGA1_c05080 at 501.119 kb on - strand, within PGA1_c05080 at 501.119 kb on - strand, within PGA1_c05080 at 501.119 kb on - strand, within PGA1_c05080 at 501.119 kb on - strand, within PGA1_c05080 at 501.119 kb on - strand, within PGA1_c05080 at 501.119 kb on - strand, within PGA1_c05080 at 501.127 kb on - strand, within PGA1_c05080 at 501.145 kb on + strand, within PGA1_c05080 at 501.152 kb on + strand, within PGA1_c05080 at 501.152 kb on - strand, within PGA1_c05080 at 501.152 kb on - strand, within PGA1_c05080 at 501.153 kb on - strand, within PGA1_c05080 at 501.160 kb on - strand, within PGA1_c05080 at 501.162 kb on - strand, within PGA1_c05080 at 501.179 kb on + strand, within PGA1_c05080 at 501.203 kb on + strand, within PGA1_c05080 at 501.232 kb on - strand, within PGA1_c05080 at 501.259 kb on - strand, within PGA1_c05080 at 501.318 kb on + strand at 501.360 kb on - strand at 501.370 kb on - strand at 501.370 kb on - strand at 501.386 kb on + strand at 501.386 kb on + strand at 501.388 kb on + strand at 501.388 kb on + strand at 501.388 kb on + strand at 501.388 kb on + strand at 501.388 kb on + strand at 501.388 kb on + strand at 501.388 kb on + strand at 501.388 kb on + strand at 501.388 kb on + strand at 501.388 kb on + strand at 501.391 kb on - strand at 501.394 kb on - strand at 501.395 kb on + strand at 501.396 kb on - strand at 501.396 kb on - strand at 501.396 kb on - strand at 501.396 kb on - strand at 501.396 kb on - strand at 501.396 kb on - strand at 501.396 kb on - strand at 501.396 kb on - strand at 501.405 kb on + strand at 501.413 kb on - strand at 501.418 kb on - strand at 501.439 kb on - strand at 501.466 kb on + strand at 501.476 kb on - strand at 501.486 kb on - strand at 501.494 kb on - strand at 501.496 kb on + strand at 501.496 kb on + strand at 501.496 kb on + strand at 501.496 kb on + strand at 501.496 kb on + strand at 501.504 kb on - strand at 501.522 kb on + strand at 501.522 kb on + strand at 501.552 kb on + strand at 501.552 kb on + strand at 501.552 kb on + strand at 501.560 kb on - strand at 501.560 kb on - strand at 501.560 kb on - strand at 501.560 kb on - strand at 501.560 kb on - strand at 501.560 kb on - strand at 501.560 kb on - strand at 501.560 kb on - strand at 501.567 kb on - strand at 501.604 kb on + strand at 501.629 kb on - strand at 501.630 kb on + strand at 501.641 kb on - strand at 501.646 kb on + strand at 501.647 kb on - strand at 501.654 kb on - strand at 501.662 kb on + strand at 501.670 kb on - strand at 501.670 kb on - strand at 501.699 kb on + strand at 501.699 kb on - strand at 501.747 kb on + strand at 501.760 kb on - strand at 501.792 kb on - strand at 501.792 kb on - strand at 501.802 kb on + strand at 501.810 kb on + strand at 501.810 kb on + strand at 501.810 kb on + strand at 501.810 kb on - strand at 501.810 kb on - strand at 501.818 kb on - strand at 501.818 kb on - strand at 501.828 kb on - strand at 501.892 kb on + strand, within PGA1_c05090 at 501.899 kb on + strand, within PGA1_c05090 at 501.938 kb on + strand, within PGA1_c05090 at 501.945 kb on + strand, within PGA1_c05090 at 501.945 kb on + strand, within PGA1_c05090 at 501.945 kb on + strand, within PGA1_c05090 at 501.953 kb on - strand, within PGA1_c05090 at 501.963 kb on - strand, within PGA1_c05090 at 502.012 kb on + strand, within PGA1_c05090 at 502.064 kb on - strand, within PGA1_c05090 at 502.066 kb on - strand, within PGA1_c05090 at 502.068 kb on + strand, within PGA1_c05090 at 502.090 kb on + strand, within PGA1_c05090 at 502.090 kb on + strand, within PGA1_c05090 at 502.090 kb on + strand, within PGA1_c05090 at 502.090 kb on + strand, within PGA1_c05090 at 502.090 kb on + strand, within PGA1_c05090 at 502.098 kb on - strand, within PGA1_c05090 at 502.098 kb on - strand, within PGA1_c05090 at 502.127 kb on - strand, within PGA1_c05090 at 502.285 kb on + strand, within PGA1_c05090 at 502.285 kb on + strand, within PGA1_c05090 at 502.286 kb on + strand, within PGA1_c05090 at 502.291 kb on + strand, within PGA1_c05090 at 502.294 kb on - strand, within PGA1_c05090 at 502.294 kb on - strand, within PGA1_c05090 at 502.294 kb on - strand, within PGA1_c05090 at 502.357 kb on + strand, within PGA1_c05090 at 502.357 kb on + strand, within PGA1_c05090 at 502.357 kb on + strand, within PGA1_c05090 at 502.394 kb on + strand, within PGA1_c05090 at 502.399 kb on + strand, within PGA1_c05090 at 502.399 kb on + strand, within PGA1_c05090 at 502.399 kb on + strand, within PGA1_c05090 at 502.399 kb on + strand, within PGA1_c05090 at 502.399 kb on + strand, within PGA1_c05090 at 502.399 kb on + strand, within PGA1_c05090 at 502.399 kb on + strand, within PGA1_c05090 at 502.399 kb on + strand, within PGA1_c05090 at 502.399 kb on + strand, within PGA1_c05090 at 502.404 kb on + strand, within PGA1_c05090 at 502.404 kb on + strand, within PGA1_c05090 at 502.404 kb on + strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.407 kb on - strand, within PGA1_c05090 at 502.409 kb on + strand, within PGA1_c05090 at 502.409 kb on + strand, within PGA1_c05090 at 502.409 kb on + strand, within PGA1_c05090 at 502.409 kb on + strand, within PGA1_c05090 at 502.409 kb on + strand, within PGA1_c05090 at 502.409 kb on + strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.417 kb on - strand, within PGA1_c05090 at 502.643 kb on + strand at 502.651 kb on - strand at 502.651 kb on - strand at 502.660 kb on + strand at 502.660 kb on + strand at 502.697 kb on + strand at 502.722 kb on - strand at 502.745 kb on - strand at 502.751 kb on + strand at 502.751 kb on - strand at 502.759 kb on - strand at 502.759 kb on - strand at 502.783 kb on + strand at 502.808 kb on - strand, within PGA1_c05100 at 502.810 kb on + strand, within PGA1_c05100 at 502.825 kb on - strand, within PGA1_c05100 at 502.864 kb on + strand, within PGA1_c05100 at 502.872 kb on - strand, within PGA1_c05100 at 502.872 kb on - strand, within PGA1_c05100 at 502.890 kb on - strand, within PGA1_c05100 at 502.958 kb on + strand, within PGA1_c05100 at 503.016 kb on + strand, within PGA1_c05100 at 503.017 kb on - strand, within PGA1_c05100 at 503.019 kb on - strand, within PGA1_c05100 at 503.019 kb on - strand, within PGA1_c05100 at 503.053 kb on - strand, within PGA1_c05100 at 503.067 kb on + strand, within PGA1_c05100 at 503.096 kb on - strand, within PGA1_c05100 at 503.118 kb on + strand, within PGA1_c05100 at 503.118 kb on + strand, within PGA1_c05100 at 503.118 kb on + strand, within PGA1_c05100 at 503.177 kb on + strand, within PGA1_c05100 at 503.185 kb on + strand, within PGA1_c05100 at 503.193 kb on + strand, within PGA1_c05100 at 503.200 kb on + strand, within PGA1_c05100 at 503.321 kb on + strand, within PGA1_c05100 at 503.337 kb on - strand at 503.371 kb on + strand at 503.420 kb on - strand at 503.420 kb on - strand at 503.422 kb on + strand at 503.441 kb on - strand at 503.441 kb on - strand at 503.510 kb on + strand at 503.518 kb on - strand at 503.544 kb on + strand at 503.555 kb on + strand at 503.571 kb on + strand at 503.607 kb on - strand at 503.619 kb on - strand at 503.624 kb on - strand at 503.690 kb on - strand, within PGA1_c05110 at 503.692 kb on + strand, within PGA1_c05110 at 503.692 kb on + strand, within PGA1_c05110 at 503.692 kb on + strand, within PGA1_c05110 at 503.692 kb on + strand, within PGA1_c05110 at 503.700 kb on - strand, within PGA1_c05110 at 503.700 kb on - strand, within PGA1_c05110 at 503.700 kb on - strand, within PGA1_c05110
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 500,787 + PGA1_c05070 0.89 -2.3 500,797 + PGA1_c05070 0.90 -3.4 500,797 + PGA1_c05070 0.90 +2.7 500,797 + PGA1_c05070 0.90 +3.3 500,805 - -1.7 500,813 + +1.5 500,831 - -1.7 500,831 - +0.1 500,834 + -1.5 500,880 + -1.4 500,888 - +0.2 500,910 + -0.0 500,958 - PGA1_c05080 0.11 -1.4 500,958 - PGA1_c05080 0.11 -1.4 500,973 - PGA1_c05080 0.15 -2.4 500,980 + PGA1_c05080 0.17 -0.9 500,981 + PGA1_c05080 0.17 +0.2 500,989 + PGA1_c05080 0.19 -2.4 500,995 - PGA1_c05080 0.20 -0.8 501,095 + PGA1_c05080 0.43 +0.7 501,100 + PGA1_c05080 0.44 -1.8 501,100 + PGA1_c05080 0.44 -2.5 501,111 + PGA1_c05080 0.47 -1.1 501,111 + PGA1_c05080 0.47 -2.1 501,111 + PGA1_c05080 0.47 -0.8 501,111 + PGA1_c05080 0.47 -2.1 501,111 + PGA1_c05080 0.47 -1.4 501,111 + PGA1_c05080 0.47 -3.2 501,111 + PGA1_c05080 0.47 +0.7 501,111 + PGA1_c05080 0.47 -2.4 501,119 - PGA1_c05080 0.49 -2.6 501,119 - PGA1_c05080 0.49 -1.4 501,119 - PGA1_c05080 0.49 +1.5 501,119 - PGA1_c05080 0.49 +0.7 501,119 - PGA1_c05080 0.49 +1.0 501,119 - PGA1_c05080 0.49 +1.0 501,119 - PGA1_c05080 0.49 -2.1 501,127 - PGA1_c05080 0.50 -2.3 501,145 + PGA1_c05080 0.54 -0.3 501,152 + PGA1_c05080 0.56 +1.9 501,152 - PGA1_c05080 0.56 -1.4 501,152 - PGA1_c05080 0.56 -2.2 501,153 - PGA1_c05080 0.56 -2.2 501,160 - PGA1_c05080 0.58 -0.6 501,162 - PGA1_c05080 0.58 +1.4 501,179 + PGA1_c05080 0.62 -2.3 501,203 + PGA1_c05080 0.68 -1.8 501,232 - PGA1_c05080 0.74 -3.2 501,259 - PGA1_c05080 0.81 -0.9 501,318 + -0.4 501,360 - +2.8 501,370 - -0.6 501,370 - -2.8 501,386 + +1.1 501,386 + -2.2 501,388 + -1.2 501,388 + +2.9 501,388 + +1.8 501,388 + -1.6 501,388 + -1.8 501,388 + -2.6 501,388 + -0.7 501,388 + -0.2 501,388 + -1.5 501,388 + -1.6 501,391 - -0.9 501,394 - -1.3 501,395 + +0.7 501,396 - -2.1 501,396 - -0.2 501,396 - +0.0 501,396 - -1.2 501,396 - -2.9 501,396 - -2.1 501,396 - +0.8 501,396 - -2.0 501,405 + -1.2 501,413 - -2.0 501,418 - +1.8 501,439 - +0.0 501,466 + +1.3 501,476 - +3.0 501,486 - -1.7 501,494 - -2.6 501,496 + +1.1 501,496 + +3.5 501,496 + +1.2 501,496 + -2.2 501,496 + -2.8 501,504 - -1.4 501,522 + +2.3 501,522 + +2.0 501,552 + +2.4 501,552 + +1.4 501,552 + -1.8 501,560 - +2.7 501,560 - +2.1 501,560 - -2.7 501,560 - -0.8 501,560 - +1.9 501,560 - -3.3 501,560 - +0.6 501,560 - -1.1 501,567 - +0.7 501,604 + -1.3 501,629 - +1.6 501,630 + -1.0 501,641 - -1.9 501,646 + -2.5 501,647 - -1.3 501,654 - +0.1 501,662 + -2.2 501,670 - -1.4 501,670 - +2.3 501,699 + +0.9 501,699 - -2.4 501,747 + -3.3 501,760 - -0.3 501,792 - -2.6 501,792 - -1.5 501,802 + -0.9 501,810 + +0.2 501,810 + -0.5 501,810 + -2.3 501,810 - -1.3 501,810 - -1.1 501,818 - +2.3 501,818 - +1.4 501,828 - -1.6 501,892 + PGA1_c05090 0.13 +2.4 501,899 + PGA1_c05090 0.14 -1.0 501,938 + PGA1_c05090 0.18 -0.5 501,945 + PGA1_c05090 0.19 -1.6 501,945 + PGA1_c05090 0.19 +0.0 501,945 + PGA1_c05090 0.19 -1.1 501,953 - PGA1_c05090 0.20 -1.1 501,963 - PGA1_c05090 0.21 -0.6 502,012 + PGA1_c05090 0.26 -0.6 502,064 - PGA1_c05090 0.31 +1.5 502,066 - PGA1_c05090 0.32 -1.6 502,068 + PGA1_c05090 0.32 +0.3 502,090 + PGA1_c05090 0.34 -2.5 502,090 + PGA1_c05090 0.34 +1.4 502,090 + PGA1_c05090 0.34 -1.2 502,090 + PGA1_c05090 0.34 +1.8 502,090 + PGA1_c05090 0.34 +3.4 502,098 - PGA1_c05090 0.35 -1.6 502,098 - PGA1_c05090 0.35 -1.0 502,127 - PGA1_c05090 0.38 -0.7 502,285 + PGA1_c05090 0.55 -2.0 502,285 + PGA1_c05090 0.55 -1.0 502,286 + PGA1_c05090 0.55 +1.3 502,291 + PGA1_c05090 0.55 -2.3 502,294 - PGA1_c05090 0.56 -1.5 502,294 - PGA1_c05090 0.56 +2.4 502,294 - PGA1_c05090 0.56 +0.9 502,357 + PGA1_c05090 0.62 +0.0 502,357 + PGA1_c05090 0.62 -3.3 502,357 + PGA1_c05090 0.62 +1.5 502,394 + PGA1_c05090 0.66 -1.4 502,399 + PGA1_c05090 0.67 -0.6 502,399 + PGA1_c05090 0.67 +0.3 502,399 + PGA1_c05090 0.67 -0.7 502,399 + PGA1_c05090 0.67 +2.5 502,399 + PGA1_c05090 0.67 -0.8 502,399 + PGA1_c05090 0.67 +0.7 502,399 + PGA1_c05090 0.67 -1.7 502,399 + PGA1_c05090 0.67 -1.1 502,399 + PGA1_c05090 0.67 -0.4 502,404 + PGA1_c05090 0.67 -0.3 502,404 + PGA1_c05090 0.67 +0.4 502,404 + PGA1_c05090 0.67 -1.5 502,407 - PGA1_c05090 0.68 -0.4 502,407 - PGA1_c05090 0.68 +1.0 502,407 - PGA1_c05090 0.68 -0.3 502,407 - PGA1_c05090 0.68 -0.1 502,407 - PGA1_c05090 0.68 +1.1 502,407 - PGA1_c05090 0.68 -1.8 502,407 - PGA1_c05090 0.68 -1.8 502,407 - PGA1_c05090 0.68 -0.9 502,407 - PGA1_c05090 0.68 +0.5 502,407 - PGA1_c05090 0.68 +1.0 502,407 - PGA1_c05090 0.68 -0.6 502,409 + PGA1_c05090 0.68 +0.9 502,409 + PGA1_c05090 0.68 -1.6 502,409 + PGA1_c05090 0.68 -1.7 502,409 + PGA1_c05090 0.68 -0.9 502,409 + PGA1_c05090 0.68 +2.2 502,409 + PGA1_c05090 0.68 -3.3 502,417 - PGA1_c05090 0.69 -0.6 502,417 - PGA1_c05090 0.69 +1.8 502,417 - PGA1_c05090 0.69 +0.7 502,417 - PGA1_c05090 0.69 -1.2 502,417 - PGA1_c05090 0.69 -0.7 502,417 - PGA1_c05090 0.69 -3.1 502,417 - PGA1_c05090 0.69 -2.1 502,417 - PGA1_c05090 0.69 +1.5 502,417 - PGA1_c05090 0.69 +2.1 502,417 - PGA1_c05090 0.69 +0.3 502,417 - PGA1_c05090 0.69 +0.6 502,643 + -0.3 502,651 - -1.7 502,651 - -3.2 502,660 + -0.7 502,660 + +0.3 502,697 + -0.8 502,722 - -1.6 502,745 - -1.0 502,751 + -1.0 502,751 - +1.0 502,759 - -1.0 502,759 - -0.9 502,783 + -0.5 502,808 - PGA1_c05100 0.10 +2.7 502,810 + PGA1_c05100 0.11 -1.3 502,825 - PGA1_c05100 0.13 -0.4 502,864 + PGA1_c05100 0.19 +1.0 502,872 - PGA1_c05100 0.20 -0.8 502,872 - PGA1_c05100 0.20 -0.9 502,890 - PGA1_c05100 0.23 +1.4 502,958 + PGA1_c05100 0.33 +2.0 503,016 + PGA1_c05100 0.42 -0.1 503,017 - PGA1_c05100 0.42 +1.2 503,019 - PGA1_c05100 0.42 +0.5 503,019 - PGA1_c05100 0.42 +2.5 503,053 - PGA1_c05100 0.47 +3.1 503,067 + PGA1_c05100 0.49 +1.6 503,096 - PGA1_c05100 0.54 -0.8 503,118 + PGA1_c05100 0.57 -2.1 503,118 + PGA1_c05100 0.57 -2.1 503,118 + PGA1_c05100 0.57 +1.0 503,177 + PGA1_c05100 0.66 -1.2 503,185 + PGA1_c05100 0.67 -3.1 503,193 + PGA1_c05100 0.68 -1.4 503,200 + PGA1_c05100 0.69 +1.0 503,321 + PGA1_c05100 0.88 -1.0 503,337 - -0.4 503,371 + +1.2 503,420 - -1.4 503,420 - -0.7 503,422 + -0.0 503,441 - +2.1 503,441 - -1.8 503,510 + +0.5 503,518 - -2.0 503,544 + +1.8 503,555 + -1.5 503,571 + -2.2 503,607 - +2.6 503,619 - +0.8 503,624 - -1.6 503,690 - PGA1_c05110 0.13 -0.5 503,692 + PGA1_c05110 0.13 -1.2 503,692 + PGA1_c05110 0.13 -2.8 503,692 + PGA1_c05110 0.13 +0.7 503,692 + PGA1_c05110 0.13 +1.8 503,700 - PGA1_c05110 0.14 -1.3 503,700 - PGA1_c05110 0.14 +1.5 503,700 - PGA1_c05110 0.14 -0.6
Or see this region's nucleotide sequence