Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04870 and PGA1_c04880 are separated by 77 nucleotides PGA1_c04880 and PGA1_c04890 are separated by 9 nucleotides PGA1_c04890 and PGA1_c04900 are separated by 77 nucleotides
PGA1_c04870: PGA1_c04870 - tryptophan synthase beta chain, at 478,592 to 479,845
_c04870
PGA1_c04880: PGA1_c04880 - Uncharacterized protein conserved in bacteria, at 479,923 to 480,306
_c04880
PGA1_c04890: PGA1_c04890 - putative beta-lactamase, at 480,316 to 481,479
_c04890
PGA1_c04900: PGA1_c04900 - peptidyl-tRNA hydrolase Pth, at 481,557 to 482,315
_c04900
Position (kb) 480
481
482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 479.813 kb on - strand at 479.844 kb on + strand at 479.852 kb on - strand at 479.852 kb on - strand at 479.904 kb on + strand at 479.914 kb on + strand at 479.921 kb on + strand at 479.929 kb on - strand at 479.929 kb on - strand at 479.938 kb on - strand at 479.951 kb on - strand at 479.963 kb on - strand, within PGA1_c04880 at 480.033 kb on - strand, within PGA1_c04880 at 480.033 kb on - strand, within PGA1_c04880 at 480.033 kb on - strand, within PGA1_c04880 at 480.038 kb on - strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.063 kb on + strand, within PGA1_c04880 at 480.073 kb on + strand, within PGA1_c04880 at 480.073 kb on + strand, within PGA1_c04880 at 480.097 kb on + strand, within PGA1_c04880 at 480.119 kb on + strand, within PGA1_c04880 at 480.131 kb on - strand, within PGA1_c04880 at 480.173 kb on + strand, within PGA1_c04880 at 480.180 kb on + strand, within PGA1_c04880 at 480.188 kb on - strand, within PGA1_c04880 at 480.188 kb on - strand, within PGA1_c04880 at 480.188 kb on - strand, within PGA1_c04880 at 480.191 kb on + strand, within PGA1_c04880 at 480.198 kb on - strand, within PGA1_c04880 at 480.198 kb on - strand, within PGA1_c04880 at 480.199 kb on - strand, within PGA1_c04880 at 480.199 kb on - strand, within PGA1_c04880 at 480.199 kb on - strand, within PGA1_c04880 at 480.232 kb on - strand, within PGA1_c04880 at 480.267 kb on + strand, within PGA1_c04880 at 480.270 kb on - strand at 480.282 kb on + strand at 480.290 kb on - strand at 480.295 kb on + strand at 480.308 kb on + strand at 480.313 kb on + strand at 480.323 kb on + strand at 480.392 kb on + strand at 480.397 kb on + strand at 480.400 kb on - strand at 480.405 kb on - strand at 480.426 kb on + strand at 480.438 kb on - strand, within PGA1_c04890 at 480.455 kb on + strand, within PGA1_c04890 at 480.463 kb on - strand, within PGA1_c04890 at 480.516 kb on - strand, within PGA1_c04890 at 480.540 kb on - strand, within PGA1_c04890 at 480.550 kb on + strand, within PGA1_c04890 at 480.558 kb on - strand, within PGA1_c04890 at 480.583 kb on - strand, within PGA1_c04890 at 480.593 kb on + strand, within PGA1_c04890 at 480.661 kb on + strand, within PGA1_c04890 at 480.666 kb on + strand, within PGA1_c04890 at 480.678 kb on + strand, within PGA1_c04890 at 480.681 kb on - strand, within PGA1_c04890 at 480.692 kb on + strand, within PGA1_c04890 at 480.696 kb on + strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.803 kb on - strand, within PGA1_c04890 at 480.891 kb on + strand, within PGA1_c04890 at 480.898 kb on + strand, within PGA1_c04890 at 480.899 kb on - strand, within PGA1_c04890 at 480.906 kb on - strand, within PGA1_c04890 at 480.934 kb on + strand, within PGA1_c04890 at 480.970 kb on + strand, within PGA1_c04890 at 480.978 kb on - strand, within PGA1_c04890 at 480.978 kb on - strand, within PGA1_c04890 at 481.007 kb on + strand, within PGA1_c04890 at 481.079 kb on + strand, within PGA1_c04890 at 481.142 kb on + strand, within PGA1_c04890 at 481.199 kb on - strand, within PGA1_c04890 at 481.249 kb on + strand, within PGA1_c04890 at 481.249 kb on + strand, within PGA1_c04890 at 481.259 kb on + strand, within PGA1_c04890 at 481.274 kb on - strand, within PGA1_c04890 at 481.274 kb on - strand, within PGA1_c04890 at 481.284 kb on - strand, within PGA1_c04890 at 481.284 kb on - strand, within PGA1_c04890 at 481.363 kb on - strand at 481.376 kb on + strand at 481.404 kb on + strand at 481.411 kb on + strand at 481.411 kb on + strand at 481.419 kb on - strand at 481.424 kb on + strand at 481.479 kb on + strand at 481.497 kb on - strand at 481.497 kb on - strand at 481.497 kb on - strand at 481.497 kb on - strand at 481.498 kb on + strand at 481.506 kb on - strand at 481.506 kb on - strand at 481.508 kb on - strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.514 kb on + strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.522 kb on - strand at 481.588 kb on - strand at 481.611 kb on + strand at 481.614 kb on + strand at 481.614 kb on + strand at 481.619 kb on - strand at 481.619 kb on - strand at 481.622 kb on - strand at 481.629 kb on - strand at 481.629 kb on - strand at 481.666 kb on - strand, within PGA1_c04900 at 481.711 kb on - strand, within PGA1_c04900 at 481.750 kb on + strand, within PGA1_c04900 at 481.763 kb on + strand, within PGA1_c04900 at 482.327 kb on - strand at 482.327 kb on - strand at 482.327 kb on - strand at 482.329 kb on - strand at 482.341 kb on - strand at 482.350 kb on + strand at 482.350 kb on + strand at 482.350 kb on + strand at 482.350 kb on + strand at 482.358 kb on + strand at 482.358 kb on + strand at 482.358 kb on + strand at 482.358 kb on - strand at 482.358 kb on - strand at 482.366 kb on - strand at 482.366 kb on - strand at 482.399 kb on + strand at 482.419 kb on - strand at 482.421 kb on + strand at 482.435 kb on + strand at 482.435 kb on + strand at 482.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 479,813 - -1.1 479,844 + -0.8 479,852 - +0.6 479,852 - +2.1 479,904 + -2.5 479,914 + -2.2 479,921 + -2.2 479,929 - +2.1 479,929 - -1.2 479,938 - +1.5 479,951 - -2.1 479,963 - PGA1_c04880 0.10 -0.5 480,033 - PGA1_c04880 0.29 -0.8 480,033 - PGA1_c04880 0.29 -2.0 480,033 - PGA1_c04880 0.29 -0.5 480,038 - PGA1_c04880 0.30 -1.0 480,063 + PGA1_c04880 0.36 -1.2 480,063 + PGA1_c04880 0.36 +1.5 480,063 + PGA1_c04880 0.36 +1.7 480,063 + PGA1_c04880 0.36 -0.7 480,073 + PGA1_c04880 0.39 -2.2 480,073 + PGA1_c04880 0.39 +1.3 480,097 + PGA1_c04880 0.45 +1.9 480,119 + PGA1_c04880 0.51 +2.4 480,131 - PGA1_c04880 0.54 -2.6 480,173 + PGA1_c04880 0.65 -1.9 480,180 + PGA1_c04880 0.67 -3.0 480,188 - PGA1_c04880 0.69 +2.0 480,188 - PGA1_c04880 0.69 -0.2 480,188 - PGA1_c04880 0.69 +2.4 480,191 + PGA1_c04880 0.70 +2.1 480,198 - PGA1_c04880 0.72 -0.0 480,198 - PGA1_c04880 0.72 -2.0 480,199 - PGA1_c04880 0.72 -2.6 480,199 - PGA1_c04880 0.72 +2.5 480,199 - PGA1_c04880 0.72 -0.3 480,232 - PGA1_c04880 0.80 -0.5 480,267 + PGA1_c04880 0.90 -1.3 480,270 - +2.5 480,282 + +0.8 480,290 - +1.8 480,295 + +2.2 480,308 + +1.5 480,313 + +1.0 480,323 + -0.6 480,392 + -1.1 480,397 + -1.6 480,400 - -2.2 480,405 - +1.9 480,426 + +0.5 480,438 - PGA1_c04890 0.10 -1.2 480,455 + PGA1_c04890 0.12 +1.5 480,463 - PGA1_c04890 0.13 +0.4 480,516 - PGA1_c04890 0.17 -2.7 480,540 - PGA1_c04890 0.19 +1.6 480,550 + PGA1_c04890 0.20 -0.8 480,558 - PGA1_c04890 0.21 -2.2 480,583 - PGA1_c04890 0.23 +1.1 480,593 + PGA1_c04890 0.24 +2.5 480,661 + PGA1_c04890 0.30 +1.0 480,666 + PGA1_c04890 0.30 +1.9 480,678 + PGA1_c04890 0.31 -0.5 480,681 - PGA1_c04890 0.31 -2.7 480,692 + PGA1_c04890 0.32 +1.6 480,696 + PGA1_c04890 0.33 -1.5 480,803 - PGA1_c04890 0.42 -2.6 480,803 - PGA1_c04890 0.42 -2.4 480,803 - PGA1_c04890 0.42 +2.4 480,803 - PGA1_c04890 0.42 +3.2 480,803 - PGA1_c04890 0.42 +1.5 480,803 - PGA1_c04890 0.42 +2.0 480,803 - PGA1_c04890 0.42 +2.3 480,803 - PGA1_c04890 0.42 +2.8 480,891 + PGA1_c04890 0.49 -0.5 480,898 + PGA1_c04890 0.50 +0.7 480,899 - PGA1_c04890 0.50 -1.9 480,906 - PGA1_c04890 0.51 +1.9 480,934 + PGA1_c04890 0.53 +2.1 480,970 + PGA1_c04890 0.56 +2.2 480,978 - PGA1_c04890 0.57 -1.6 480,978 - PGA1_c04890 0.57 +1.5 481,007 + PGA1_c04890 0.59 -2.5 481,079 + PGA1_c04890 0.66 -2.3 481,142 + PGA1_c04890 0.71 -1.6 481,199 - PGA1_c04890 0.76 +0.3 481,249 + PGA1_c04890 0.80 -2.4 481,249 + PGA1_c04890 0.80 +1.9 481,259 + PGA1_c04890 0.81 -3.6 481,274 - PGA1_c04890 0.82 -0.5 481,274 - PGA1_c04890 0.82 -0.5 481,284 - PGA1_c04890 0.83 -1.2 481,284 - PGA1_c04890 0.83 +2.5 481,363 - +0.3 481,376 + +0.5 481,404 + -1.7 481,411 + -1.1 481,411 + -1.9 481,419 - +1.1 481,424 + -0.4 481,479 + -1.6 481,497 - +3.2 481,497 - +2.7 481,497 - +1.0 481,497 - +2.0 481,498 + +0.7 481,506 - -1.2 481,506 - -0.0 481,508 - +1.0 481,514 + -2.4 481,514 + -1.1 481,514 + -2.3 481,514 + -0.7 481,514 + +0.6 481,514 + -2.1 481,522 - +1.8 481,522 - +1.5 481,522 - +2.9 481,522 - -1.6 481,522 - +1.5 481,522 - -1.5 481,522 - -1.8 481,522 - +1.7 481,588 - -1.4 481,611 + -1.4 481,614 + -1.5 481,614 + +2.9 481,619 - +1.0 481,619 - -1.9 481,622 - +1.1 481,629 - -0.4 481,629 - -1.0 481,666 - PGA1_c04900 0.14 -1.8 481,711 - PGA1_c04900 0.20 -1.4 481,750 + PGA1_c04900 0.25 -1.7 481,763 + PGA1_c04900 0.27 +2.3 482,327 - -1.8 482,327 - +2.3 482,327 - +1.0 482,329 - +3.1 482,341 - -1.8 482,350 + +1.1 482,350 + +4.6 482,350 + +2.2 482,350 + +2.6 482,358 + -1.5 482,358 + -0.2 482,358 + -2.2 482,358 - +2.2 482,358 - -0.8 482,366 - +2.1 482,366 - +2.9 482,399 + +0.3 482,419 - +4.3 482,421 + -0.2 482,435 + -2.3 482,435 + +2.6 482,443 - +1.8
Or see this region's nucleotide sequence