Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03880 and PGA1_c03890 are separated by 94 nucleotides PGA1_c03890 and PGA1_c03900 are separated by 108 nucleotides PGA1_c03900 and PGA1_c03910 overlap by 4 nucleotides PGA1_c03910 and PGA1_c03920 are separated by 95 nucleotides
PGA1_c03880: PGA1_c03880 - putative transcriptional activator protein RaiR, at 374,695 to 375,414
_c03880
PGA1_c03890: PGA1_c03890 - putative acyl-homoserine-lactone synthase RaiI, at 375,509 to 376,150
_c03890
PGA1_c03900: PGA1_c03900 - hypothetical protein, at 376,259 to 377,797
_c03900
PGA1_c03910: PGA1_c03910 - short chain dehydrogenase / reductase, at 377,794 to 378,552
_c03910
PGA1_c03920: PGA1_c03920 - Putative transmembrane protein (Alph_Pro_TM)., at 378,648 to 379,538
_c03920
Position (kb) 376
377
378 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 375.265 kb on + strand, within PGA1_c03880 at 375.265 kb on + strand, within PGA1_c03880 at 375.265 kb on + strand, within PGA1_c03880 at 375.265 kb on + strand, within PGA1_c03880 at 375.265 kb on + strand, within PGA1_c03880 at 375.273 kb on - strand, within PGA1_c03880 at 375.273 kb on - strand, within PGA1_c03880 at 375.273 kb on - strand, within PGA1_c03880 at 375.273 kb on - strand, within PGA1_c03880 at 375.273 kb on - strand, within PGA1_c03880 at 375.273 kb on - strand, within PGA1_c03880 at 375.327 kb on + strand, within PGA1_c03880 at 375.342 kb on + strand, within PGA1_c03880 at 375.355 kb on + strand at 375.362 kb on + strand at 375.365 kb on + strand at 375.365 kb on + strand at 375.365 kb on - strand at 375.367 kb on + strand at 375.370 kb on - strand at 375.370 kb on - strand at 375.373 kb on - strand at 375.375 kb on - strand at 375.375 kb on - strand at 375.375 kb on - strand at 375.392 kb on + strand at 375.392 kb on + strand at 375.392 kb on + strand at 375.392 kb on + strand at 375.392 kb on + strand at 375.392 kb on + strand at 375.392 kb on + strand at 375.392 kb on + strand at 375.392 kb on + strand at 375.392 kb on + strand at 375.400 kb on - strand at 375.400 kb on - strand at 375.400 kb on - strand at 375.402 kb on + strand at 375.442 kb on + strand at 375.449 kb on - strand at 375.498 kb on - strand at 375.499 kb on + strand at 375.499 kb on + strand at 375.512 kb on - strand at 375.534 kb on + strand at 375.534 kb on + strand at 375.534 kb on + strand at 375.542 kb on - strand at 375.542 kb on - strand at 375.542 kb on - strand at 375.542 kb on - strand at 375.542 kb on - strand at 375.547 kb on - strand at 375.587 kb on - strand, within PGA1_c03890 at 375.587 kb on - strand, within PGA1_c03890 at 375.587 kb on - strand, within PGA1_c03890 at 375.589 kb on + strand, within PGA1_c03890 at 375.606 kb on + strand, within PGA1_c03890 at 375.647 kb on - strand, within PGA1_c03890 at 375.648 kb on + strand, within PGA1_c03890 at 375.665 kb on - strand, within PGA1_c03890 at 375.737 kb on - strand, within PGA1_c03890 at 375.737 kb on - strand, within PGA1_c03890 at 375.767 kb on - strand, within PGA1_c03890 at 375.769 kb on + strand, within PGA1_c03890 at 375.777 kb on - strand, within PGA1_c03890 at 375.777 kb on - strand, within PGA1_c03890 at 375.828 kb on + strand, within PGA1_c03890 at 375.844 kb on - strand, within PGA1_c03890 at 375.862 kb on + strand, within PGA1_c03890 at 375.865 kb on - strand, within PGA1_c03890 at 375.865 kb on - strand, within PGA1_c03890 at 375.872 kb on + strand, within PGA1_c03890 at 375.874 kb on + strand, within PGA1_c03890 at 375.922 kb on - strand, within PGA1_c03890 at 375.957 kb on + strand, within PGA1_c03890 at 375.957 kb on + strand, within PGA1_c03890 at 375.993 kb on + strand, within PGA1_c03890 at 376.002 kb on + strand, within PGA1_c03890 at 376.026 kb on + strand, within PGA1_c03890 at 376.045 kb on - strand, within PGA1_c03890 at 376.057 kb on + strand, within PGA1_c03890 at 376.078 kb on + strand, within PGA1_c03890 at 376.086 kb on - strand at 376.123 kb on + strand at 376.123 kb on + strand at 376.174 kb on - strand at 376.180 kb on + strand at 376.183 kb on - strand at 376.188 kb on - strand at 376.188 kb on - strand at 376.188 kb on - strand at 376.368 kb on - strand at 376.441 kb on + strand, within PGA1_c03900 at 376.444 kb on + strand, within PGA1_c03900 at 376.452 kb on - strand, within PGA1_c03900 at 376.526 kb on - strand, within PGA1_c03900 at 376.532 kb on - strand, within PGA1_c03900 at 376.606 kb on + strand, within PGA1_c03900 at 376.618 kb on + strand, within PGA1_c03900 at 376.654 kb on + strand, within PGA1_c03900 at 376.654 kb on - strand, within PGA1_c03900 at 376.703 kb on + strand, within PGA1_c03900 at 376.703 kb on + strand, within PGA1_c03900 at 376.711 kb on - strand, within PGA1_c03900 at 376.711 kb on - strand, within PGA1_c03900 at 376.711 kb on - strand, within PGA1_c03900 at 376.711 kb on - strand, within PGA1_c03900 at 376.730 kb on - strand, within PGA1_c03900 at 377.040 kb on + strand, within PGA1_c03900 at 377.122 kb on + strand, within PGA1_c03900 at 377.123 kb on + strand, within PGA1_c03900 at 377.176 kb on - strand, within PGA1_c03900 at 377.203 kb on + strand, within PGA1_c03900 at 377.234 kb on + strand, within PGA1_c03900 at 377.261 kb on + strand, within PGA1_c03900 at 377.261 kb on + strand, within PGA1_c03900 at 377.268 kb on + strand, within PGA1_c03900 at 377.335 kb on + strand, within PGA1_c03900 at 377.335 kb on + strand, within PGA1_c03900 at 377.335 kb on + strand, within PGA1_c03900 at 377.335 kb on + strand, within PGA1_c03900 at 377.335 kb on + strand, within PGA1_c03900 at 377.343 kb on - strand, within PGA1_c03900 at 377.343 kb on - strand, within PGA1_c03900 at 377.343 kb on - strand, within PGA1_c03900 at 377.343 kb on - strand, within PGA1_c03900 at 377.343 kb on - strand, within PGA1_c03900 at 377.366 kb on - strand, within PGA1_c03900 at 377.415 kb on + strand, within PGA1_c03900 at 377.415 kb on + strand, within PGA1_c03900 at 377.517 kb on + strand, within PGA1_c03900 at 377.529 kb on + strand, within PGA1_c03900 at 377.547 kb on - strand, within PGA1_c03900 at 377.554 kb on - strand, within PGA1_c03900 at 377.554 kb on - strand, within PGA1_c03900 at 377.619 kb on - strand, within PGA1_c03900 at 377.665 kb on + strand at 377.675 kb on + strand at 377.683 kb on - strand at 377.726 kb on - strand at 377.747 kb on + strand at 377.747 kb on + strand at 377.755 kb on - strand at 377.767 kb on - strand at 377.767 kb on - strand at 377.798 kb on + strand at 377.813 kb on - strand at 377.835 kb on - strand at 377.918 kb on + strand, within PGA1_c03910 at 377.926 kb on - strand, within PGA1_c03910 at 377.927 kb on + strand, within PGA1_c03910 at 377.935 kb on - strand, within PGA1_c03910 at 377.959 kb on + strand, within PGA1_c03910 at 377.988 kb on + strand, within PGA1_c03910 at 377.988 kb on + strand, within PGA1_c03910 at 377.988 kb on + strand, within PGA1_c03910 at 377.996 kb on - strand, within PGA1_c03910 at 378.001 kb on + strand, within PGA1_c03910 at 378.037 kb on - strand, within PGA1_c03910 at 378.086 kb on + strand, within PGA1_c03910 at 378.210 kb on - strand, within PGA1_c03910 at 378.254 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.304 kb on - strand, within PGA1_c03910 at 378.306 kb on + strand, within PGA1_c03910 at 378.316 kb on - strand, within PGA1_c03910 at 378.399 kb on + strand, within PGA1_c03910 at 378.399 kb on + strand, within PGA1_c03910 at 378.399 kb on + strand, within PGA1_c03910 at 378.407 kb on - strand, within PGA1_c03910 at 378.407 kb on - strand, within PGA1_c03910 at 378.407 kb on - strand, within PGA1_c03910 at 378.427 kb on + strand, within PGA1_c03910 at 378.452 kb on - strand, within PGA1_c03910 at 378.481 kb on - strand at 378.626 kb on + strand at 378.644 kb on - strand at 378.725 kb on - strand at 378.773 kb on + strand, within PGA1_c03920 at 378.773 kb on + strand, within PGA1_c03920
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 375,265 + PGA1_c03880 0.79 -2.2 375,265 + PGA1_c03880 0.79 -3.2 375,265 + PGA1_c03880 0.79 -2.7 375,265 + PGA1_c03880 0.79 -0.2 375,265 + PGA1_c03880 0.79 -1.6 375,273 - PGA1_c03880 0.80 -1.5 375,273 - PGA1_c03880 0.80 -3.5 375,273 - PGA1_c03880 0.80 -2.9 375,273 - PGA1_c03880 0.80 +0.8 375,273 - PGA1_c03880 0.80 +0.7 375,273 - PGA1_c03880 0.80 -4.5 375,327 + PGA1_c03880 0.88 -1.9 375,342 + PGA1_c03880 0.90 -0.9 375,355 + -1.4 375,362 + +1.1 375,365 + -0.1 375,365 + -0.0 375,365 - -0.2 375,367 + -1.7 375,370 - +0.1 375,370 - -2.4 375,373 - -0.7 375,375 - +0.6 375,375 - -2.6 375,375 - -2.5 375,392 + +0.8 375,392 + -1.0 375,392 + -2.1 375,392 + -1.7 375,392 + -2.9 375,392 + -2.5 375,392 + -2.8 375,392 + -1.7 375,392 + -3.7 375,392 + +1.0 375,400 - -1.4 375,400 - -0.5 375,400 - -2.2 375,402 + -2.6 375,442 + -1.9 375,449 - -0.5 375,498 - +2.8 375,499 + +1.6 375,499 + -1.3 375,512 - -0.0 375,534 + -2.4 375,534 + -3.6 375,534 + -2.7 375,542 - +0.8 375,542 - +2.9 375,542 - -2.2 375,542 - -1.8 375,542 - +2.1 375,547 - -2.0 375,587 - PGA1_c03890 0.12 +2.1 375,587 - PGA1_c03890 0.12 -1.7 375,587 - PGA1_c03890 0.12 -0.7 375,589 + PGA1_c03890 0.12 -2.6 375,606 + PGA1_c03890 0.15 +1.5 375,647 - PGA1_c03890 0.21 -1.8 375,648 + PGA1_c03890 0.22 +1.8 375,665 - PGA1_c03890 0.24 -0.2 375,737 - PGA1_c03890 0.36 +0.9 375,737 - PGA1_c03890 0.36 -1.0 375,767 - PGA1_c03890 0.40 +2.6 375,769 + PGA1_c03890 0.40 +0.7 375,777 - PGA1_c03890 0.42 +2.8 375,777 - PGA1_c03890 0.42 -1.6 375,828 + PGA1_c03890 0.50 -1.1 375,844 - PGA1_c03890 0.52 -1.3 375,862 + PGA1_c03890 0.55 +4.2 375,865 - PGA1_c03890 0.55 +0.5 375,865 - PGA1_c03890 0.55 -2.1 375,872 + PGA1_c03890 0.57 -3.1 375,874 + PGA1_c03890 0.57 +2.1 375,922 - PGA1_c03890 0.64 +1.9 375,957 + PGA1_c03890 0.70 +1.3 375,957 + PGA1_c03890 0.70 +0.4 375,993 + PGA1_c03890 0.75 -1.0 376,002 + PGA1_c03890 0.77 +1.5 376,026 + PGA1_c03890 0.81 -0.7 376,045 - PGA1_c03890 0.83 +2.3 376,057 + PGA1_c03890 0.85 -2.1 376,078 + PGA1_c03890 0.89 +1.7 376,086 - -1.6 376,123 + -1.1 376,123 + -0.7 376,174 - -0.5 376,180 + -0.5 376,183 - +1.8 376,188 - +3.1 376,188 - -2.2 376,188 - +0.8 376,368 - +1.7 376,441 + PGA1_c03900 0.12 -2.4 376,444 + PGA1_c03900 0.12 +1.4 376,452 - PGA1_c03900 0.13 +0.8 376,526 - PGA1_c03900 0.17 -0.8 376,532 - PGA1_c03900 0.18 -2.1 376,606 + PGA1_c03900 0.23 +0.1 376,618 + PGA1_c03900 0.23 +1.5 376,654 + PGA1_c03900 0.26 -1.0 376,654 - PGA1_c03900 0.26 -0.5 376,703 + PGA1_c03900 0.29 +1.2 376,703 + PGA1_c03900 0.29 -1.7 376,711 - PGA1_c03900 0.29 -2.0 376,711 - PGA1_c03900 0.29 -1.8 376,711 - PGA1_c03900 0.29 -0.7 376,711 - PGA1_c03900 0.29 -1.7 376,730 - PGA1_c03900 0.31 -0.9 377,040 + PGA1_c03900 0.51 +4.0 377,122 + PGA1_c03900 0.56 -0.1 377,123 + PGA1_c03900 0.56 -1.4 377,176 - PGA1_c03900 0.60 -2.9 377,203 + PGA1_c03900 0.61 +3.8 377,234 + PGA1_c03900 0.63 +1.8 377,261 + PGA1_c03900 0.65 -3.6 377,261 + PGA1_c03900 0.65 +0.6 377,268 + PGA1_c03900 0.66 -0.5 377,335 + PGA1_c03900 0.70 -1.3 377,335 + PGA1_c03900 0.70 +0.6 377,335 + PGA1_c03900 0.70 -0.6 377,335 + PGA1_c03900 0.70 -1.6 377,335 + PGA1_c03900 0.70 +0.6 377,343 - PGA1_c03900 0.70 -1.5 377,343 - PGA1_c03900 0.70 +1.3 377,343 - PGA1_c03900 0.70 +2.6 377,343 - PGA1_c03900 0.70 -0.7 377,343 - PGA1_c03900 0.70 +1.5 377,366 - PGA1_c03900 0.72 +0.3 377,415 + PGA1_c03900 0.75 -1.0 377,415 + PGA1_c03900 0.75 +2.0 377,517 + PGA1_c03900 0.82 -1.9 377,529 + PGA1_c03900 0.83 +1.0 377,547 - PGA1_c03900 0.84 -1.5 377,554 - PGA1_c03900 0.84 +1.3 377,554 - PGA1_c03900 0.84 -2.3 377,619 - PGA1_c03900 0.88 +0.5 377,665 + -0.4 377,675 + -2.4 377,683 - -1.1 377,726 - +0.4 377,747 + -2.9 377,747 + -1.3 377,755 - +0.6 377,767 - +3.9 377,767 - +2.0 377,798 + +1.5 377,813 - -1.2 377,835 - +3.0 377,918 + PGA1_c03910 0.16 -2.3 377,926 - PGA1_c03910 0.17 -1.1 377,927 + PGA1_c03910 0.18 -1.5 377,935 - PGA1_c03910 0.19 -0.7 377,959 + PGA1_c03910 0.22 -0.5 377,988 + PGA1_c03910 0.26 -1.8 377,988 + PGA1_c03910 0.26 +1.1 377,988 + PGA1_c03910 0.26 -2.4 377,996 - PGA1_c03910 0.27 -0.1 378,001 + PGA1_c03910 0.27 -2.8 378,037 - PGA1_c03910 0.32 +2.5 378,086 + PGA1_c03910 0.38 +3.5 378,210 - PGA1_c03910 0.55 -0.1 378,254 - PGA1_c03910 0.61 -0.2 378,304 - PGA1_c03910 0.67 -1.5 378,304 - PGA1_c03910 0.67 -1.4 378,304 - PGA1_c03910 0.67 +1.2 378,304 - PGA1_c03910 0.67 +1.3 378,304 - PGA1_c03910 0.67 +1.9 378,306 + PGA1_c03910 0.67 -0.3 378,316 - PGA1_c03910 0.69 -0.0 378,399 + PGA1_c03910 0.80 -0.8 378,399 + PGA1_c03910 0.80 -2.5 378,399 + PGA1_c03910 0.80 +3.1 378,407 - PGA1_c03910 0.81 +1.3 378,407 - PGA1_c03910 0.81 +0.2 378,407 - PGA1_c03910 0.81 +2.0 378,427 + PGA1_c03910 0.83 -0.2 378,452 - PGA1_c03910 0.87 +1.7 378,481 - -2.3 378,626 + -2.0 378,644 - +1.8 378,725 - -0.6 378,773 + PGA1_c03920 0.14 +0.7 378,773 + PGA1_c03920 0.14 -1.2
Or see this region's nucleotide sequence