Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00360 and PGA1_262p00370 are separated by 812 nucleotides PGA1_262p00370 and PGA1_262p00380 are separated by 120 nucleotides
PGA1_262p00360: PGA1_262p00360 - methyl-accepting chemotaxis protein, at 40,851 to 43,001
_262p00360
PGA1_262p00370: PGA1_262p00370 - putative polysaccharide synthase, at 43,814 to 45,307
_262p00370
PGA1_262p00380: PGA1_262p00380 - sugar transferase, at 45,428 to 46,111
_262p00380
Position (kb) 43
44
45
46 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 42.848 kb on - strand at 43.043 kb on + strand at 43.065 kb on + strand at 43.069 kb on + strand at 43.105 kb on + strand at 43.113 kb on - strand at 43.113 kb on - strand at 43.122 kb on + strand at 43.235 kb on - strand at 43.345 kb on + strand at 43.374 kb on + strand at 43.382 kb on - strand at 43.456 kb on + strand at 43.475 kb on + strand at 43.595 kb on - strand at 43.747 kb on + strand at 43.815 kb on - strand at 43.968 kb on - strand, within PGA1_262p00370 at 44.017 kb on - strand, within PGA1_262p00370 at 44.027 kb on - strand, within PGA1_262p00370 at 44.251 kb on - strand, within PGA1_262p00370 at 44.368 kb on - strand, within PGA1_262p00370 at 44.421 kb on - strand, within PGA1_262p00370 at 44.491 kb on - strand, within PGA1_262p00370 at 44.566 kb on - strand, within PGA1_262p00370 at 44.760 kb on + strand, within PGA1_262p00370 at 44.773 kb on - strand, within PGA1_262p00370 at 45.037 kb on - strand, within PGA1_262p00370 at 45.047 kb on - strand, within PGA1_262p00370 at 45.096 kb on - strand, within PGA1_262p00370 at 45.277 kb on + strand at 45.279 kb on + strand at 45.365 kb on + strand at 45.392 kb on + strand at 45.392 kb on + strand at 45.400 kb on - strand at 45.402 kb on + strand at 45.402 kb on + strand at 45.402 kb on + strand at 45.402 kb on + strand at 45.407 kb on + strand at 45.407 kb on + strand at 45.407 kb on + strand at 45.410 kb on - strand at 45.413 kb on + strand at 45.413 kb on + strand at 45.428 kb on + strand at 45.535 kb on + strand, within PGA1_262p00380 at 45.543 kb on - strand, within PGA1_262p00380 at 45.565 kb on - strand, within PGA1_262p00380 at 45.608 kb on - strand, within PGA1_262p00380 at 45.769 kb on + strand, within PGA1_262p00380 at 45.788 kb on + strand, within PGA1_262p00380 at 45.796 kb on - strand, within PGA1_262p00380 at 45.881 kb on + strand, within PGA1_262p00380 at 45.890 kb on - strand, within PGA1_262p00380 at 46.146 kb on - strand at 46.149 kb on + strand at 46.254 kb on - strand at 46.290 kb on - strand at 46.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 42,848 - -2.6 43,043 + +3.1 43,065 + -2.0 43,069 + +1.8 43,105 + +2.7 43,113 - +0.8 43,113 - -0.0 43,122 + +1.8 43,235 - -1.1 43,345 + -0.5 43,374 + +1.6 43,382 - -0.7 43,456 + +0.7 43,475 + +2.4 43,595 - +1.3 43,747 + -1.2 43,815 - -1.2 43,968 - PGA1_262p00370 0.10 +4.1 44,017 - PGA1_262p00370 0.14 -1.7 44,027 - PGA1_262p00370 0.14 -1.1 44,251 - PGA1_262p00370 0.29 +1.5 44,368 - PGA1_262p00370 0.37 +2.5 44,421 - PGA1_262p00370 0.41 +1.5 44,491 - PGA1_262p00370 0.45 -0.8 44,566 - PGA1_262p00370 0.50 +1.1 44,760 + PGA1_262p00370 0.63 +2.4 44,773 - PGA1_262p00370 0.64 -1.1 45,037 - PGA1_262p00370 0.82 -2.2 45,047 - PGA1_262p00370 0.83 +1.8 45,096 - PGA1_262p00370 0.86 -1.0 45,277 + +1.7 45,279 + +1.5 45,365 + -1.2 45,392 + +1.7 45,392 + +1.2 45,400 - -2.2 45,402 + -1.2 45,402 + -2.9 45,402 + +2.8 45,402 + -0.5 45,407 + +1.0 45,407 + +0.4 45,407 + +1.5 45,410 - +1.1 45,413 + +0.3 45,413 + +2.8 45,428 + -2.2 45,535 + PGA1_262p00380 0.16 -2.0 45,543 - PGA1_262p00380 0.17 -1.2 45,565 - PGA1_262p00380 0.20 -1.2 45,608 - PGA1_262p00380 0.26 -0.1 45,769 + PGA1_262p00380 0.50 -1.4 45,788 + PGA1_262p00380 0.53 +1.1 45,796 - PGA1_262p00380 0.54 +0.8 45,881 + PGA1_262p00380 0.66 +1.2 45,890 - PGA1_262p00380 0.68 +1.4 46,146 - -2.4 46,149 + -1.8 46,254 - -3.0 46,290 - -0.7 46,290 - -0.9
Or see this region's nucleotide sequence