Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c31410 and PGA1_c31420 are separated by 120 nucleotides PGA1_c31420 and PGA1_c31430 overlap by 8 nucleotides PGA1_c31430 and PGA1_c31440 are separated by 59 nucleotides
PGA1_c31410: PGA1_c31410 - putative receptor family ligand-binding protein, at 3,282,104 to 3,283,288
_c31410
PGA1_c31420: PGA1_c31420 - branched-chain amino acid transport system ATP-binding protein, at 3,283,409 to 3,284,185
_c31420
PGA1_c31430: PGA1_c31430 - branched-chain amino acid transport system ATP-binding protein, at 3,284,178 to 3,284,927
_c31430
PGA1_c31440: PGA1_c31440 - branched-chain amino acid transport system permease protein, at 3,284,987 to 3,286,018
_c31440
Position (kb)
3283
3284
3285 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3282.409 kb on + strand, within PGA1_c31410 at 3282.422 kb on - strand, within PGA1_c31410 at 3282.422 kb on - strand, within PGA1_c31410 at 3282.422 kb on - strand, within PGA1_c31410 at 3282.430 kb on + strand, within PGA1_c31410 at 3282.484 kb on - strand, within PGA1_c31410 at 3282.546 kb on + strand, within PGA1_c31410 at 3282.546 kb on + strand, within PGA1_c31410 at 3282.546 kb on + strand, within PGA1_c31410 at 3282.551 kb on - strand, within PGA1_c31410 at 3282.562 kb on + strand, within PGA1_c31410 at 3282.616 kb on + strand, within PGA1_c31410 at 3282.616 kb on + strand, within PGA1_c31410 at 3282.624 kb on - strand, within PGA1_c31410 at 3282.624 kb on - strand, within PGA1_c31410 at 3282.624 kb on - strand, within PGA1_c31410 at 3282.624 kb on - strand, within PGA1_c31410 at 3282.707 kb on + strand, within PGA1_c31410 at 3282.798 kb on - strand, within PGA1_c31410 at 3282.811 kb on + strand, within PGA1_c31410 at 3282.844 kb on - strand, within PGA1_c31410 at 3282.844 kb on - strand, within PGA1_c31410 at 3282.888 kb on + strand, within PGA1_c31410 at 3282.907 kb on + strand, within PGA1_c31410 at 3282.939 kb on + strand, within PGA1_c31410 at 3282.939 kb on + strand, within PGA1_c31410 at 3282.947 kb on - strand, within PGA1_c31410 at 3282.952 kb on - strand, within PGA1_c31410 at 3282.995 kb on + strand, within PGA1_c31410 at 3283.085 kb on - strand, within PGA1_c31410 at 3283.115 kb on + strand, within PGA1_c31410 at 3283.130 kb on + strand, within PGA1_c31410 at 3283.131 kb on + strand, within PGA1_c31410 at 3283.138 kb on - strand, within PGA1_c31410 at 3283.139 kb on - strand, within PGA1_c31410 at 3283.235 kb on + strand at 3283.304 kb on - strand at 3283.305 kb on + strand at 3283.313 kb on - strand at 3283.313 kb on - strand at 3283.313 kb on - strand at 3283.328 kb on - strand at 3283.328 kb on - strand at 3283.348 kb on - strand at 3283.356 kb on + strand at 3283.405 kb on + strand at 3283.413 kb on - strand at 3283.413 kb on - strand at 3283.421 kb on + strand at 3283.421 kb on + strand at 3283.421 kb on + strand at 3283.421 kb on + strand at 3283.429 kb on - strand at 3283.429 kb on - strand at 3283.429 kb on - strand at 3283.439 kb on - strand at 3283.467 kb on - strand at 3283.477 kb on + strand at 3283.482 kb on + strand at 3283.497 kb on + strand, within PGA1_c31420 at 3283.497 kb on + strand, within PGA1_c31420 at 3283.497 kb on + strand, within PGA1_c31420 at 3283.505 kb on - strand, within PGA1_c31420 at 3283.570 kb on + strand, within PGA1_c31420 at 3283.570 kb on + strand, within PGA1_c31420 at 3283.578 kb on - strand, within PGA1_c31420 at 3283.579 kb on - strand, within PGA1_c31420 at 3283.604 kb on + strand, within PGA1_c31420 at 3283.726 kb on + strand, within PGA1_c31420 at 3283.726 kb on + strand, within PGA1_c31420 at 3283.729 kb on - strand, within PGA1_c31420 at 3283.729 kb on - strand, within PGA1_c31420 at 3283.729 kb on - strand, within PGA1_c31420 at 3283.734 kb on - strand, within PGA1_c31420 at 3283.737 kb on + strand, within PGA1_c31420 at 3283.763 kb on - strand, within PGA1_c31420 at 3283.829 kb on + strand, within PGA1_c31420 at 3283.829 kb on - strand, within PGA1_c31420 at 3283.830 kb on + strand, within PGA1_c31420 at 3283.845 kb on + strand, within PGA1_c31420 at 3283.845 kb on + strand, within PGA1_c31420 at 3283.845 kb on + strand, within PGA1_c31420 at 3283.853 kb on - strand, within PGA1_c31420 at 3283.853 kb on - strand, within PGA1_c31420 at 3283.855 kb on + strand, within PGA1_c31420 at 3283.920 kb on - strand, within PGA1_c31420 at 3283.920 kb on - strand, within PGA1_c31420 at 3283.955 kb on - strand, within PGA1_c31420 at 3283.965 kb on - strand, within PGA1_c31420 at 3283.968 kb on + strand, within PGA1_c31420 at 3283.976 kb on - strand, within PGA1_c31420 at 3284.002 kb on - strand, within PGA1_c31420 at 3284.020 kb on - strand, within PGA1_c31420 at 3284.039 kb on - strand, within PGA1_c31420 at 3284.082 kb on - strand, within PGA1_c31420 at 3284.148 kb on - strand at 3284.157 kb on + strand at 3284.168 kb on + strand at 3284.179 kb on + strand at 3284.179 kb on + strand at 3284.187 kb on - strand at 3284.189 kb on + strand at 3284.195 kb on + strand at 3284.197 kb on - strand at 3284.197 kb on - strand at 3284.220 kb on - strand at 3284.257 kb on + strand, within PGA1_c31430 at 3284.263 kb on - strand, within PGA1_c31430 at 3284.338 kb on + strand, within PGA1_c31430 at 3284.351 kb on - strand, within PGA1_c31430 at 3284.409 kb on - strand, within PGA1_c31430 at 3284.445 kb on + strand, within PGA1_c31430 at 3284.453 kb on - strand, within PGA1_c31430 at 3284.508 kb on + strand, within PGA1_c31430 at 3284.528 kb on - strand, within PGA1_c31430 at 3284.544 kb on + strand, within PGA1_c31430 at 3284.544 kb on + strand, within PGA1_c31430 at 3284.552 kb on - strand, within PGA1_c31430 at 3284.584 kb on - strand, within PGA1_c31430 at 3284.691 kb on + strand, within PGA1_c31430 at 3284.691 kb on + strand, within PGA1_c31430 at 3284.699 kb on - strand, within PGA1_c31430 at 3284.705 kb on - strand, within PGA1_c31430 at 3284.705 kb on - strand, within PGA1_c31430 at 3284.714 kb on + strand, within PGA1_c31430 at 3284.768 kb on - strand, within PGA1_c31430 at 3284.769 kb on - strand, within PGA1_c31430 at 3284.837 kb on - strand, within PGA1_c31430 at 3284.905 kb on - strand at 3284.934 kb on - strand at 3284.952 kb on - strand at 3284.989 kb on + strand at 3285.005 kb on - strand at 3285.010 kb on - strand at 3285.112 kb on - strand, within PGA1_c31440 at 3285.112 kb on - strand, within PGA1_c31440 at 3285.123 kb on + strand, within PGA1_c31440 at 3285.182 kb on + strand, within PGA1_c31440 at 3285.185 kb on - strand, within PGA1_c31440
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 3,282,409 + PGA1_c31410 0.26 -3.4 3,282,422 - PGA1_c31410 0.27 +2.8 3,282,422 - PGA1_c31410 0.27 -2.5 3,282,422 - PGA1_c31410 0.27 +3.3 3,282,430 + PGA1_c31410 0.28 +0.0 3,282,484 - PGA1_c31410 0.32 -0.3 3,282,546 + PGA1_c31410 0.37 +1.2 3,282,546 + PGA1_c31410 0.37 -3.2 3,282,546 + PGA1_c31410 0.37 +3.8 3,282,551 - PGA1_c31410 0.38 +1.0 3,282,562 + PGA1_c31410 0.39 +0.2 3,282,616 + PGA1_c31410 0.43 -2.7 3,282,616 + PGA1_c31410 0.43 +2.4 3,282,624 - PGA1_c31410 0.44 -0.1 3,282,624 - PGA1_c31410 0.44 -2.0 3,282,624 - PGA1_c31410 0.44 +3.1 3,282,624 - PGA1_c31410 0.44 +2.6 3,282,707 + PGA1_c31410 0.51 +1.9 3,282,798 - PGA1_c31410 0.59 -1.9 3,282,811 + PGA1_c31410 0.60 -2.3 3,282,844 - PGA1_c31410 0.62 -1.9 3,282,844 - PGA1_c31410 0.62 -2.8 3,282,888 + PGA1_c31410 0.66 -2.2 3,282,907 + PGA1_c31410 0.68 +2.6 3,282,939 + PGA1_c31410 0.70 -1.7 3,282,939 + PGA1_c31410 0.70 -1.5 3,282,947 - PGA1_c31410 0.71 +1.5 3,282,952 - PGA1_c31410 0.72 +1.9 3,282,995 + PGA1_c31410 0.75 +3.3 3,283,085 - PGA1_c31410 0.83 +2.8 3,283,115 + PGA1_c31410 0.85 +3.3 3,283,130 + PGA1_c31410 0.87 +2.6 3,283,131 + PGA1_c31410 0.87 +2.8 3,283,138 - PGA1_c31410 0.87 +1.4 3,283,139 - PGA1_c31410 0.87 +1.2 3,283,235 + +2.2 3,283,304 - -1.0 3,283,305 + +1.9 3,283,313 - -2.6 3,283,313 - -0.4 3,283,313 - +1.9 3,283,328 - +0.6 3,283,328 - +3.1 3,283,348 - +2.0 3,283,356 + -1.7 3,283,405 + -2.5 3,283,413 - -0.7 3,283,413 - -1.0 3,283,421 + +0.5 3,283,421 + -2.4 3,283,421 + +0.8 3,283,421 + -0.4 3,283,429 - -2.9 3,283,429 - +2.3 3,283,429 - +2.4 3,283,439 - -0.8 3,283,467 - +0.2 3,283,477 + +2.7 3,283,482 + -0.8 3,283,497 + PGA1_c31420 0.11 +1.0 3,283,497 + PGA1_c31420 0.11 -1.2 3,283,497 + PGA1_c31420 0.11 +2.3 3,283,505 - PGA1_c31420 0.12 -0.0 3,283,570 + PGA1_c31420 0.21 -2.1 3,283,570 + PGA1_c31420 0.21 +0.9 3,283,578 - PGA1_c31420 0.22 +1.7 3,283,579 - PGA1_c31420 0.22 +0.0 3,283,604 + PGA1_c31420 0.25 +1.5 3,283,726 + PGA1_c31420 0.41 +1.9 3,283,726 + PGA1_c31420 0.41 -1.4 3,283,729 - PGA1_c31420 0.41 -2.5 3,283,729 - PGA1_c31420 0.41 -1.6 3,283,729 - PGA1_c31420 0.41 +0.8 3,283,734 - PGA1_c31420 0.42 +3.0 3,283,737 + PGA1_c31420 0.42 -0.1 3,283,763 - PGA1_c31420 0.46 +2.0 3,283,829 + PGA1_c31420 0.54 +1.9 3,283,829 - PGA1_c31420 0.54 -2.1 3,283,830 + PGA1_c31420 0.54 -2.6 3,283,845 + PGA1_c31420 0.56 +2.5 3,283,845 + PGA1_c31420 0.56 +3.4 3,283,845 + PGA1_c31420 0.56 -1.7 3,283,853 - PGA1_c31420 0.57 +1.1 3,283,853 - PGA1_c31420 0.57 -1.3 3,283,855 + PGA1_c31420 0.57 +0.5 3,283,920 - PGA1_c31420 0.66 -2.4 3,283,920 - PGA1_c31420 0.66 +2.3 3,283,955 - PGA1_c31420 0.70 +0.8 3,283,965 - PGA1_c31420 0.72 +2.8 3,283,968 + PGA1_c31420 0.72 +2.0 3,283,976 - PGA1_c31420 0.73 +4.4 3,284,002 - PGA1_c31420 0.76 -2.2 3,284,020 - PGA1_c31420 0.79 -2.0 3,284,039 - PGA1_c31420 0.81 +0.9 3,284,082 - PGA1_c31420 0.87 +2.0 3,284,148 - -2.7 3,284,157 + -1.0 3,284,168 + -1.4 3,284,179 + +1.7 3,284,179 + +0.3 3,284,187 - +3.2 3,284,189 + -2.1 3,284,195 + +1.2 3,284,197 - +3.3 3,284,197 - -1.7 3,284,220 - +2.8 3,284,257 + PGA1_c31430 0.11 +1.4 3,284,263 - PGA1_c31430 0.11 +0.6 3,284,338 + PGA1_c31430 0.21 +1.1 3,284,351 - PGA1_c31430 0.23 -0.5 3,284,409 - PGA1_c31430 0.31 +1.2 3,284,445 + PGA1_c31430 0.36 +1.5 3,284,453 - PGA1_c31430 0.37 -2.4 3,284,508 + PGA1_c31430 0.44 +1.6 3,284,528 - PGA1_c31430 0.47 -1.8 3,284,544 + PGA1_c31430 0.49 +2.4 3,284,544 + PGA1_c31430 0.49 -1.7 3,284,552 - PGA1_c31430 0.50 -0.8 3,284,584 - PGA1_c31430 0.54 +1.0 3,284,691 + PGA1_c31430 0.68 +2.1 3,284,691 + PGA1_c31430 0.68 -2.1 3,284,699 - PGA1_c31430 0.69 -1.5 3,284,705 - PGA1_c31430 0.70 +1.5 3,284,705 - PGA1_c31430 0.70 +2.4 3,284,714 + PGA1_c31430 0.71 -1.8 3,284,768 - PGA1_c31430 0.79 -1.9 3,284,769 - PGA1_c31430 0.79 -1.7 3,284,837 - PGA1_c31430 0.88 +0.4 3,284,905 - -2.1 3,284,934 - +2.9 3,284,952 - -2.5 3,284,989 + -1.2 3,285,005 - +1.0 3,285,010 - +1.7 3,285,112 - PGA1_c31440 0.12 -2.0 3,285,112 - PGA1_c31440 0.12 +2.3 3,285,123 + PGA1_c31440 0.13 +0.9 3,285,182 + PGA1_c31440 0.19 -0.2 3,285,185 - PGA1_c31440 0.19 -2.4
Or see this region's nucleotide sequence