Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03160 and PGA1_c03170 are separated by 24 nucleotides PGA1_c03170 and PGA1_c03180 are separated by 82 nucleotides PGA1_c03180 and PGA1_c03190 are separated by 88 nucleotides PGA1_c03190 and PGA1_c03200 are separated by 8 nucleotides
PGA1_c03160: PGA1_c03160 - metallo-beta-lactamase-like protein, at 305,300 to 306,337
_c03160
PGA1_c03170: PGA1_c03170 - hypothetical protein, at 306,362 to 306,826
_c03170
PGA1_c03180: PGA1_c03180 - hypothetical protein, at 306,909 to 307,394
_c03180
PGA1_c03190: PGA1_c03190 - Bacterial aa3 type cytochrome c oxidase subunit IV., at 307,483 to 307,617
_c03190
PGA1_c03200: PGA1_c03200 - hypothetical protein, at 307,626 to 308,294
_c03200
Position (kb)
306
307 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 305.422 kb on + strand, within PGA1_c03160 at 305.422 kb on + strand, within PGA1_c03160 at 305.430 kb on - strand, within PGA1_c03160 at 305.430 kb on - strand, within PGA1_c03160 at 305.430 kb on - strand, within PGA1_c03160 at 305.464 kb on - strand, within PGA1_c03160 at 305.466 kb on - strand, within PGA1_c03160 at 305.472 kb on - strand, within PGA1_c03160 at 305.480 kb on + strand, within PGA1_c03160 at 305.512 kb on + strand, within PGA1_c03160 at 305.618 kb on - strand, within PGA1_c03160 at 305.625 kb on - strand, within PGA1_c03160 at 305.636 kb on + strand, within PGA1_c03160 at 305.642 kb on - strand, within PGA1_c03160 at 305.644 kb on - strand, within PGA1_c03160 at 305.677 kb on + strand, within PGA1_c03160 at 305.792 kb on - strand, within PGA1_c03160 at 305.851 kb on + strand, within PGA1_c03160 at 305.877 kb on + strand, within PGA1_c03160 at 305.891 kb on + strand, within PGA1_c03160 at 305.923 kb on - strand, within PGA1_c03160 at 306.014 kb on - strand, within PGA1_c03160 at 306.037 kb on + strand, within PGA1_c03160 at 306.047 kb on + strand, within PGA1_c03160 at 306.073 kb on - strand, within PGA1_c03160 at 306.099 kb on + strand, within PGA1_c03160 at 306.163 kb on + strand, within PGA1_c03160 at 306.233 kb on + strand, within PGA1_c03160 at 306.238 kb on + strand at 306.285 kb on - strand at 306.325 kb on + strand at 306.332 kb on + strand at 306.358 kb on + strand at 306.358 kb on + strand at 306.371 kb on - strand at 306.395 kb on + strand at 306.407 kb on + strand at 306.415 kb on - strand, within PGA1_c03170 at 306.425 kb on - strand, within PGA1_c03170 at 306.425 kb on - strand, within PGA1_c03170 at 306.436 kb on - strand, within PGA1_c03170 at 306.516 kb on + strand, within PGA1_c03170 at 306.524 kb on - strand, within PGA1_c03170 at 306.570 kb on - strand, within PGA1_c03170 at 306.584 kb on + strand, within PGA1_c03170 at 306.592 kb on - strand, within PGA1_c03170 at 306.676 kb on + strand, within PGA1_c03170 at 306.684 kb on - strand, within PGA1_c03170 at 306.832 kb on + strand at 306.837 kb on + strand at 306.900 kb on + strand at 307.016 kb on - strand, within PGA1_c03180 at 307.026 kb on - strand, within PGA1_c03180 at 307.039 kb on + strand, within PGA1_c03180 at 307.093 kb on + strand, within PGA1_c03180 at 307.145 kb on + strand, within PGA1_c03180 at 307.335 kb on + strand, within PGA1_c03180 at 307.346 kb on + strand at 307.423 kb on + strand at 307.423 kb on + strand at 307.423 kb on + strand at 307.431 kb on - strand at 307.571 kb on - strand, within PGA1_c03190 at 307.619 kb on - strand at 307.700 kb on - strand, within PGA1_c03200 at 307.700 kb on - strand, within PGA1_c03200 at 307.750 kb on - strand, within PGA1_c03200
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 305,422 + PGA1_c03160 0.12 -0.3 305,422 + PGA1_c03160 0.12 +3.0 305,430 - PGA1_c03160 0.13 +1.8 305,430 - PGA1_c03160 0.13 -0.2 305,430 - PGA1_c03160 0.13 +2.1 305,464 - PGA1_c03160 0.16 +1.3 305,466 - PGA1_c03160 0.16 -1.2 305,472 - PGA1_c03160 0.17 -0.5 305,480 + PGA1_c03160 0.17 -1.7 305,512 + PGA1_c03160 0.20 -1.2 305,618 - PGA1_c03160 0.31 +1.6 305,625 - PGA1_c03160 0.31 -1.0 305,636 + PGA1_c03160 0.32 +2.0 305,642 - PGA1_c03160 0.33 +0.7 305,644 - PGA1_c03160 0.33 -3.6 305,677 + PGA1_c03160 0.36 +2.2 305,792 - PGA1_c03160 0.47 -0.2 305,851 + PGA1_c03160 0.53 +0.7 305,877 + PGA1_c03160 0.56 -2.0 305,891 + PGA1_c03160 0.57 -1.2 305,923 - PGA1_c03160 0.60 -1.1 306,014 - PGA1_c03160 0.69 +2.4 306,037 + PGA1_c03160 0.71 -3.4 306,047 + PGA1_c03160 0.72 +1.2 306,073 - PGA1_c03160 0.74 -1.8 306,099 + PGA1_c03160 0.77 -1.1 306,163 + PGA1_c03160 0.83 +1.2 306,233 + PGA1_c03160 0.90 +0.2 306,238 + +2.3 306,285 - -1.6 306,325 + -1.4 306,332 + +1.8 306,358 + +1.7 306,358 + -1.2 306,371 - -2.6 306,395 + -0.0 306,407 + +3.2 306,415 - PGA1_c03170 0.11 -0.5 306,425 - PGA1_c03170 0.14 -1.8 306,425 - PGA1_c03170 0.14 +1.1 306,436 - PGA1_c03170 0.16 -3.3 306,516 + PGA1_c03170 0.33 +0.5 306,524 - PGA1_c03170 0.35 -2.0 306,570 - PGA1_c03170 0.45 -1.5 306,584 + PGA1_c03170 0.48 -0.9 306,592 - PGA1_c03170 0.49 +3.2 306,676 + PGA1_c03170 0.68 -0.9 306,684 - PGA1_c03170 0.69 -0.1 306,832 + -1.7 306,837 + +0.7 306,900 + +2.7 307,016 - PGA1_c03180 0.22 -1.8 307,026 - PGA1_c03180 0.24 +1.2 307,039 + PGA1_c03180 0.27 -1.6 307,093 + PGA1_c03180 0.38 +2.8 307,145 + PGA1_c03180 0.49 -2.7 307,335 + PGA1_c03180 0.88 +1.7 307,346 + -1.1 307,423 + +1.9 307,423 + -1.5 307,423 + -1.8 307,431 - +3.1 307,571 - PGA1_c03190 0.65 -1.1 307,619 - +3.0 307,700 - PGA1_c03200 0.11 +2.6 307,700 - PGA1_c03200 0.11 +1.9 307,750 - PGA1_c03200 0.19 -2.1
Or see this region's nucleotide sequence