Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03140 and PGA1_c03150 are separated by 132 nucleotides PGA1_c03150 and PGA1_c03160 overlap by 4 nucleotides
PGA1_c03140: PGA1_c03140 - putative tRNA threonylcarbamoyladenosine biosynthesis protein, at 302,517 to 303,464
_c03140
PGA1_c03150: PGA1_c03150 - putative acyl-CoA dehydrogenase, at 303,597 to 305,303
_c03150
PGA1_c03160: PGA1_c03160 - metallo-beta-lactamase-like protein, at 305,300 to 306,337
_c03160
Position (kb)
303
304
305
306 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 303.535 kb on - strand at 303.540 kb on - strand at 303.553 kb on - strand at 303.559 kb on + strand at 303.562 kb on - strand at 303.564 kb on + strand at 303.564 kb on + strand at 303.564 kb on + strand at 303.572 kb on - strand at 303.589 kb on + strand at 303.594 kb on + strand at 303.597 kb on - strand at 303.623 kb on + strand at 303.697 kb on + strand at 303.764 kb on - strand at 303.764 kb on - strand at 303.885 kb on - strand, within PGA1_c03150 at 303.885 kb on - strand, within PGA1_c03150 at 303.885 kb on - strand, within PGA1_c03150 at 303.912 kb on + strand, within PGA1_c03150 at 303.913 kb on - strand, within PGA1_c03150 at 303.957 kb on - strand, within PGA1_c03150 at 303.972 kb on + strand, within PGA1_c03150 at 303.986 kb on + strand, within PGA1_c03150 at 304.008 kb on + strand, within PGA1_c03150 at 304.083 kb on - strand, within PGA1_c03150 at 304.097 kb on + strand, within PGA1_c03150 at 304.110 kb on - strand, within PGA1_c03150 at 304.123 kb on + strand, within PGA1_c03150 at 304.147 kb on + strand, within PGA1_c03150 at 304.175 kb on + strand, within PGA1_c03150 at 304.175 kb on + strand, within PGA1_c03150 at 304.186 kb on + strand, within PGA1_c03150 at 304.192 kb on - strand, within PGA1_c03150 at 304.192 kb on - strand, within PGA1_c03150 at 304.217 kb on - strand, within PGA1_c03150 at 304.227 kb on - strand, within PGA1_c03150 at 304.235 kb on - strand, within PGA1_c03150 at 304.296 kb on - strand, within PGA1_c03150 at 304.341 kb on - strand, within PGA1_c03150 at 304.348 kb on + strand, within PGA1_c03150 at 304.356 kb on - strand, within PGA1_c03150 at 304.358 kb on + strand, within PGA1_c03150 at 304.373 kb on - strand, within PGA1_c03150 at 304.373 kb on - strand, within PGA1_c03150 at 304.441 kb on - strand, within PGA1_c03150 at 304.449 kb on - strand, within PGA1_c03150 at 304.485 kb on - strand, within PGA1_c03150 at 304.526 kb on - strand, within PGA1_c03150 at 304.526 kb on - strand, within PGA1_c03150 at 304.530 kb on + strand, within PGA1_c03150 at 304.540 kb on + strand, within PGA1_c03150 at 304.613 kb on + strand, within PGA1_c03150 at 304.613 kb on + strand, within PGA1_c03150 at 304.627 kb on - strand, within PGA1_c03150 at 304.653 kb on + strand, within PGA1_c03150 at 304.661 kb on - strand, within PGA1_c03150 at 304.703 kb on + strand, within PGA1_c03150 at 304.703 kb on + strand, within PGA1_c03150 at 304.708 kb on + strand, within PGA1_c03150 at 304.754 kb on - strand, within PGA1_c03150 at 304.786 kb on + strand, within PGA1_c03150 at 304.794 kb on - strand, within PGA1_c03150 at 304.838 kb on + strand, within PGA1_c03150 at 304.851 kb on - strand, within PGA1_c03150 at 304.885 kb on + strand, within PGA1_c03150 at 304.899 kb on + strand, within PGA1_c03150 at 304.992 kb on + strand, within PGA1_c03150 at 304.995 kb on - strand, within PGA1_c03150 at 305.255 kb on - strand at 305.284 kb on - strand at 305.284 kb on - strand at 305.347 kb on + strand at 305.422 kb on + strand, within PGA1_c03160 at 305.422 kb on + strand, within PGA1_c03160 at 305.430 kb on - strand, within PGA1_c03160 at 305.430 kb on - strand, within PGA1_c03160 at 305.430 kb on - strand, within PGA1_c03160 at 305.464 kb on - strand, within PGA1_c03160 at 305.466 kb on - strand, within PGA1_c03160 at 305.472 kb on - strand, within PGA1_c03160 at 305.480 kb on + strand, within PGA1_c03160 at 305.512 kb on + strand, within PGA1_c03160 at 305.618 kb on - strand, within PGA1_c03160 at 305.625 kb on - strand, within PGA1_c03160 at 305.636 kb on + strand, within PGA1_c03160 at 305.642 kb on - strand, within PGA1_c03160 at 305.644 kb on - strand, within PGA1_c03160 at 305.677 kb on + strand, within PGA1_c03160 at 305.792 kb on - strand, within PGA1_c03160 at 305.851 kb on + strand, within PGA1_c03160 at 305.877 kb on + strand, within PGA1_c03160 at 305.891 kb on + strand, within PGA1_c03160 at 305.923 kb on - strand, within PGA1_c03160 at 306.014 kb on - strand, within PGA1_c03160 at 306.037 kb on + strand, within PGA1_c03160 at 306.047 kb on + strand, within PGA1_c03160 at 306.073 kb on - strand, within PGA1_c03160 at 306.099 kb on + strand, within PGA1_c03160 at 306.163 kb on + strand, within PGA1_c03160 at 306.233 kb on + strand, within PGA1_c03160 at 306.238 kb on + strand at 306.285 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 303,535 - -1.0 303,540 - +2.9 303,553 - +1.5 303,559 + -0.1 303,562 - -0.7 303,564 + -2.6 303,564 + -2.6 303,564 + -0.7 303,572 - +1.6 303,589 + -0.6 303,594 + +2.7 303,597 - -1.7 303,623 + +0.2 303,697 + -1.6 303,764 - +2.2 303,764 - -3.0 303,885 - PGA1_c03150 0.17 +1.3 303,885 - PGA1_c03150 0.17 -0.2 303,885 - PGA1_c03150 0.17 +2.1 303,912 + PGA1_c03150 0.18 -2.0 303,913 - PGA1_c03150 0.19 -2.1 303,957 - PGA1_c03150 0.21 -0.4 303,972 + PGA1_c03150 0.22 +2.0 303,986 + PGA1_c03150 0.23 +1.1 304,008 + PGA1_c03150 0.24 +2.0 304,083 - PGA1_c03150 0.28 -1.1 304,097 + PGA1_c03150 0.29 +1.9 304,110 - PGA1_c03150 0.30 +1.4 304,123 + PGA1_c03150 0.31 -1.2 304,147 + PGA1_c03150 0.32 +1.5 304,175 + PGA1_c03150 0.34 -2.3 304,175 + PGA1_c03150 0.34 +1.4 304,186 + PGA1_c03150 0.35 +1.0 304,192 - PGA1_c03150 0.35 +2.0 304,192 - PGA1_c03150 0.35 -1.4 304,217 - PGA1_c03150 0.36 +2.1 304,227 - PGA1_c03150 0.37 -0.3 304,235 - PGA1_c03150 0.37 -1.1 304,296 - PGA1_c03150 0.41 +0.3 304,341 - PGA1_c03150 0.44 +1.3 304,348 + PGA1_c03150 0.44 +3.8 304,356 - PGA1_c03150 0.44 -0.8 304,358 + PGA1_c03150 0.45 +1.7 304,373 - PGA1_c03150 0.45 -1.7 304,373 - PGA1_c03150 0.45 -1.1 304,441 - PGA1_c03150 0.49 -2.2 304,449 - PGA1_c03150 0.50 -2.2 304,485 - PGA1_c03150 0.52 -1.7 304,526 - PGA1_c03150 0.54 -2.9 304,526 - PGA1_c03150 0.54 +1.3 304,530 + PGA1_c03150 0.55 -0.9 304,540 + PGA1_c03150 0.55 -3.1 304,613 + PGA1_c03150 0.60 +3.9 304,613 + PGA1_c03150 0.60 -1.4 304,627 - PGA1_c03150 0.60 -1.8 304,653 + PGA1_c03150 0.62 +1.0 304,661 - PGA1_c03150 0.62 -2.2 304,703 + PGA1_c03150 0.65 +1.9 304,703 + PGA1_c03150 0.65 -2.1 304,708 + PGA1_c03150 0.65 +2.6 304,754 - PGA1_c03150 0.68 +2.1 304,786 + PGA1_c03150 0.70 -2.3 304,794 - PGA1_c03150 0.70 -3.0 304,838 + PGA1_c03150 0.73 -1.5 304,851 - PGA1_c03150 0.73 -1.4 304,885 + PGA1_c03150 0.75 -1.8 304,899 + PGA1_c03150 0.76 -4.3 304,992 + PGA1_c03150 0.82 +1.9 304,995 - PGA1_c03150 0.82 +0.2 305,255 - -3.1 305,284 - +2.4 305,284 - +0.1 305,347 + -2.5 305,422 + PGA1_c03160 0.12 -0.3 305,422 + PGA1_c03160 0.12 +3.0 305,430 - PGA1_c03160 0.13 +1.8 305,430 - PGA1_c03160 0.13 -0.2 305,430 - PGA1_c03160 0.13 +2.1 305,464 - PGA1_c03160 0.16 +1.3 305,466 - PGA1_c03160 0.16 -1.2 305,472 - PGA1_c03160 0.17 -0.5 305,480 + PGA1_c03160 0.17 -1.7 305,512 + PGA1_c03160 0.20 -1.2 305,618 - PGA1_c03160 0.31 +1.6 305,625 - PGA1_c03160 0.31 -1.0 305,636 + PGA1_c03160 0.32 +2.0 305,642 - PGA1_c03160 0.33 +0.7 305,644 - PGA1_c03160 0.33 -3.6 305,677 + PGA1_c03160 0.36 +2.2 305,792 - PGA1_c03160 0.47 -0.2 305,851 + PGA1_c03160 0.53 +0.7 305,877 + PGA1_c03160 0.56 -2.0 305,891 + PGA1_c03160 0.57 -1.2 305,923 - PGA1_c03160 0.60 -1.1 306,014 - PGA1_c03160 0.69 +2.4 306,037 + PGA1_c03160 0.71 -3.4 306,047 + PGA1_c03160 0.72 +1.2 306,073 - PGA1_c03160 0.74 -1.8 306,099 + PGA1_c03160 0.77 -1.1 306,163 + PGA1_c03160 0.83 +1.2 306,233 + PGA1_c03160 0.90 +0.2 306,238 + +2.3 306,285 - -1.6
Or see this region's nucleotide sequence