Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03120 and PGA1_c03130 are separated by 178 nucleotides PGA1_c03130 and PGA1_c03140 are separated by 35 nucleotides PGA1_c03140 and PGA1_c03150 are separated by 132 nucleotides
PGA1_c03120: PGA1_c03120 - hypothetical protein, at 300,891 to 301,745
_c03120
PGA1_c03130: PGA1_c03130 - Putative transcriptional regulator, at 301,924 to 302,481
_c03130
PGA1_c03140: PGA1_c03140 - putative tRNA threonylcarbamoyladenosine biosynthesis protein, at 302,517 to 303,464
_c03140
PGA1_c03150: PGA1_c03150 - putative acyl-CoA dehydrogenase, at 303,597 to 305,303
_c03150
Position (kb) 302
303
304 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 301.565 kb on + strand, within PGA1_c03120 at 301.687 kb on + strand at 301.687 kb on + strand at 301.713 kb on + strand at 301.733 kb on + strand at 301.741 kb on - strand at 301.750 kb on + strand at 301.753 kb on - strand at 301.829 kb on + strand at 301.829 kb on + strand at 301.829 kb on + strand at 301.829 kb on + strand at 301.830 kb on + strand at 301.832 kb on - strand at 301.837 kb on - strand at 301.865 kb on + strand at 301.884 kb on + strand at 301.907 kb on + strand at 301.907 kb on + strand at 301.927 kb on + strand at 301.969 kb on - strand at 302.008 kb on + strand, within PGA1_c03130 at 302.008 kb on + strand, within PGA1_c03130 at 302.008 kb on + strand, within PGA1_c03130 at 302.016 kb on - strand, within PGA1_c03130 at 302.016 kb on - strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.105 kb on + strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.113 kb on - strand, within PGA1_c03130 at 302.119 kb on + strand, within PGA1_c03130 at 302.153 kb on - strand, within PGA1_c03130 at 302.157 kb on + strand, within PGA1_c03130 at 302.165 kb on - strand, within PGA1_c03130 at 302.170 kb on - strand, within PGA1_c03130 at 302.170 kb on - strand, within PGA1_c03130 at 302.201 kb on + strand, within PGA1_c03130 at 302.223 kb on - strand, within PGA1_c03130 at 302.233 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.276 kb on + strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.284 kb on - strand, within PGA1_c03130 at 302.286 kb on + strand, within PGA1_c03130 at 302.286 kb on + strand, within PGA1_c03130 at 302.286 kb on + strand, within PGA1_c03130 at 302.286 kb on + strand, within PGA1_c03130 at 302.294 kb on - strand, within PGA1_c03130 at 302.294 kb on - strand, within PGA1_c03130 at 302.301 kb on + strand, within PGA1_c03130 at 302.301 kb on + strand, within PGA1_c03130 at 302.312 kb on + strand, within PGA1_c03130 at 302.375 kb on + strand, within PGA1_c03130 at 302.375 kb on + strand, within PGA1_c03130 at 302.438 kb on + strand at 302.454 kb on - strand at 302.463 kb on - strand at 302.465 kb on + strand at 302.470 kb on + strand at 302.503 kb on - strand at 303.535 kb on - strand at 303.540 kb on - strand at 303.553 kb on - strand at 303.559 kb on + strand at 303.562 kb on - strand at 303.564 kb on + strand at 303.564 kb on + strand at 303.564 kb on + strand at 303.572 kb on - strand at 303.589 kb on + strand at 303.594 kb on + strand at 303.597 kb on - strand at 303.623 kb on + strand at 303.697 kb on + strand at 303.764 kb on - strand at 303.764 kb on - strand at 303.885 kb on - strand, within PGA1_c03150 at 303.885 kb on - strand, within PGA1_c03150 at 303.885 kb on - strand, within PGA1_c03150 at 303.912 kb on + strand, within PGA1_c03150 at 303.913 kb on - strand, within PGA1_c03150 at 303.957 kb on - strand, within PGA1_c03150 at 303.972 kb on + strand, within PGA1_c03150 at 303.986 kb on + strand, within PGA1_c03150 at 304.008 kb on + strand, within PGA1_c03150 at 304.083 kb on - strand, within PGA1_c03150 at 304.097 kb on + strand, within PGA1_c03150 at 304.110 kb on - strand, within PGA1_c03150 at 304.123 kb on + strand, within PGA1_c03150 at 304.147 kb on + strand, within PGA1_c03150 at 304.175 kb on + strand, within PGA1_c03150 at 304.175 kb on + strand, within PGA1_c03150 at 304.186 kb on + strand, within PGA1_c03150 at 304.192 kb on - strand, within PGA1_c03150 at 304.192 kb on - strand, within PGA1_c03150 at 304.217 kb on - strand, within PGA1_c03150 at 304.227 kb on - strand, within PGA1_c03150 at 304.235 kb on - strand, within PGA1_c03150 at 304.296 kb on - strand, within PGA1_c03150 at 304.341 kb on - strand, within PGA1_c03150 at 304.348 kb on + strand, within PGA1_c03150 at 304.356 kb on - strand, within PGA1_c03150 at 304.358 kb on + strand, within PGA1_c03150 at 304.373 kb on - strand, within PGA1_c03150 at 304.373 kb on - strand, within PGA1_c03150 at 304.441 kb on - strand, within PGA1_c03150 at 304.449 kb on - strand, within PGA1_c03150
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 301,565 + PGA1_c03120 0.79 -2.8 301,687 + -1.4 301,687 + -2.0 301,713 + -2.2 301,733 + -1.4 301,741 - -2.0 301,750 + -2.9 301,753 - -1.1 301,829 + -1.8 301,829 + +1.6 301,829 + -1.9 301,829 + +2.8 301,830 + +2.0 301,832 - +3.1 301,837 - +2.4 301,865 + -0.6 301,884 + -2.7 301,907 + +2.0 301,907 + +0.7 301,927 + -2.2 301,969 - -1.9 302,008 + PGA1_c03130 0.15 +2.0 302,008 + PGA1_c03130 0.15 -1.1 302,008 + PGA1_c03130 0.15 +4.0 302,016 - PGA1_c03130 0.16 -0.3 302,016 - PGA1_c03130 0.16 -1.4 302,105 + PGA1_c03130 0.32 +2.7 302,105 + PGA1_c03130 0.32 -1.4 302,105 + PGA1_c03130 0.32 -1.2 302,105 + PGA1_c03130 0.32 -1.4 302,105 + PGA1_c03130 0.32 -1.4 302,105 + PGA1_c03130 0.32 -2.6 302,105 + PGA1_c03130 0.32 +2.0 302,105 + PGA1_c03130 0.32 +1.9 302,105 + PGA1_c03130 0.32 -0.5 302,105 + PGA1_c03130 0.32 -2.5 302,105 + PGA1_c03130 0.32 -0.5 302,105 + PGA1_c03130 0.32 -0.5 302,105 + PGA1_c03130 0.32 -0.6 302,105 + PGA1_c03130 0.32 -0.4 302,105 + PGA1_c03130 0.32 +2.2 302,105 + PGA1_c03130 0.32 -2.4 302,105 + PGA1_c03130 0.32 +1.1 302,105 + PGA1_c03130 0.32 -2.0 302,105 + PGA1_c03130 0.32 +3.3 302,113 - PGA1_c03130 0.34 -2.8 302,113 - PGA1_c03130 0.34 -2.2 302,113 - PGA1_c03130 0.34 -0.7 302,113 - PGA1_c03130 0.34 -3.6 302,113 - PGA1_c03130 0.34 +1.2 302,113 - PGA1_c03130 0.34 +2.0 302,113 - PGA1_c03130 0.34 +2.1 302,113 - PGA1_c03130 0.34 +1.5 302,113 - PGA1_c03130 0.34 +0.9 302,113 - PGA1_c03130 0.34 +2.1 302,119 + PGA1_c03130 0.35 +2.0 302,153 - PGA1_c03130 0.41 -1.6 302,157 + PGA1_c03130 0.42 -1.4 302,165 - PGA1_c03130 0.43 -1.0 302,170 - PGA1_c03130 0.44 -1.6 302,170 - PGA1_c03130 0.44 -2.1 302,201 + PGA1_c03130 0.50 -1.7 302,223 - PGA1_c03130 0.54 +0.2 302,233 + PGA1_c03130 0.55 -0.6 302,276 + PGA1_c03130 0.63 -1.0 302,276 + PGA1_c03130 0.63 -2.1 302,276 + PGA1_c03130 0.63 -0.5 302,276 + PGA1_c03130 0.63 -1.5 302,276 + PGA1_c03130 0.63 +0.1 302,276 + PGA1_c03130 0.63 -1.6 302,276 + PGA1_c03130 0.63 -0.5 302,276 + PGA1_c03130 0.63 +0.9 302,276 + PGA1_c03130 0.63 -1.1 302,276 + PGA1_c03130 0.63 -2.2 302,276 + PGA1_c03130 0.63 -2.9 302,284 - PGA1_c03130 0.65 +2.1 302,284 - PGA1_c03130 0.65 +1.2 302,284 - PGA1_c03130 0.65 +1.8 302,284 - PGA1_c03130 0.65 -1.2 302,284 - PGA1_c03130 0.65 +3.7 302,284 - PGA1_c03130 0.65 +1.2 302,284 - PGA1_c03130 0.65 +2.6 302,284 - PGA1_c03130 0.65 -2.2 302,284 - PGA1_c03130 0.65 +1.2 302,284 - PGA1_c03130 0.65 -1.2 302,284 - PGA1_c03130 0.65 -1.4 302,286 + PGA1_c03130 0.65 -1.2 302,286 + PGA1_c03130 0.65 +0.6 302,286 + PGA1_c03130 0.65 +1.9 302,286 + PGA1_c03130 0.65 -0.7 302,294 - PGA1_c03130 0.66 +3.3 302,294 - PGA1_c03130 0.66 +2.5 302,301 + PGA1_c03130 0.68 +2.6 302,301 + PGA1_c03130 0.68 +0.3 302,312 + PGA1_c03130 0.70 -1.6 302,375 + PGA1_c03130 0.81 +1.4 302,375 + PGA1_c03130 0.81 +0.3 302,438 + +0.0 302,454 - -1.6 302,463 - -0.6 302,465 + -1.4 302,470 + -0.8 302,503 - +2.2 303,535 - -1.0 303,540 - +2.9 303,553 - +1.5 303,559 + -0.1 303,562 - -0.7 303,564 + -2.6 303,564 + -2.6 303,564 + -0.7 303,572 - +1.6 303,589 + -0.6 303,594 + +2.7 303,597 - -1.7 303,623 + +0.2 303,697 + -1.6 303,764 - +2.2 303,764 - -3.0 303,885 - PGA1_c03150 0.17 +1.3 303,885 - PGA1_c03150 0.17 -0.2 303,885 - PGA1_c03150 0.17 +2.1 303,912 + PGA1_c03150 0.18 -2.0 303,913 - PGA1_c03150 0.19 -2.1 303,957 - PGA1_c03150 0.21 -0.4 303,972 + PGA1_c03150 0.22 +2.0 303,986 + PGA1_c03150 0.23 +1.1 304,008 + PGA1_c03150 0.24 +2.0 304,083 - PGA1_c03150 0.28 -1.1 304,097 + PGA1_c03150 0.29 +1.9 304,110 - PGA1_c03150 0.30 +1.4 304,123 + PGA1_c03150 0.31 -1.2 304,147 + PGA1_c03150 0.32 +1.5 304,175 + PGA1_c03150 0.34 -2.3 304,175 + PGA1_c03150 0.34 +1.4 304,186 + PGA1_c03150 0.35 +1.0 304,192 - PGA1_c03150 0.35 +2.0 304,192 - PGA1_c03150 0.35 -1.4 304,217 - PGA1_c03150 0.36 +2.1 304,227 - PGA1_c03150 0.37 -0.3 304,235 - PGA1_c03150 0.37 -1.1 304,296 - PGA1_c03150 0.41 +0.3 304,341 - PGA1_c03150 0.44 +1.3 304,348 + PGA1_c03150 0.44 +3.8 304,356 - PGA1_c03150 0.44 -0.8 304,358 + PGA1_c03150 0.45 +1.7 304,373 - PGA1_c03150 0.45 -1.7 304,373 - PGA1_c03150 0.45 -1.1 304,441 - PGA1_c03150 0.49 -2.2 304,449 - PGA1_c03150 0.50 -2.2
Or see this region's nucleotide sequence