Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c30440 and PGA1_c30450 are separated by 30 nucleotides PGA1_c30450 and PGA1_c30460 are separated by 88 nucleotides PGA1_c30460 and PGA1_c30470 are separated by 10 nucleotides
PGA1_c30440: PGA1_c30440 - putative NADP-dependent oxidoreductase, at 3,179,645 to 3,180,676
_c30440
PGA1_c30450: PGA1_c30450 - putative DJ-1/PfpI family protein, at 3,180,707 to 3,181,381
_c30450
PGA1_c30460: PGA1_c30460 - D-alanine aminotransferase Dat, at 3,181,470 to 3,182,330
_c30460
PGA1_c30470: PGA1_c30470 - mandelate racemase / muconate lactonizing enzyme, at 3,182,341 to 3,183,306
_c30470
Position (kb)
3180
3181
3182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3179.733 kb on - strand at 3179.767 kb on - strand, within PGA1_c30440 at 3179.815 kb on + strand, within PGA1_c30440 at 3179.823 kb on - strand, within PGA1_c30440 at 3179.830 kb on + strand, within PGA1_c30440 at 3179.838 kb on - strand, within PGA1_c30440 at 3179.840 kb on - strand, within PGA1_c30440 at 3179.869 kb on - strand, within PGA1_c30440 at 3179.885 kb on - strand, within PGA1_c30440 at 3179.887 kb on + strand, within PGA1_c30440 at 3179.953 kb on + strand, within PGA1_c30440 at 3180.029 kb on + strand, within PGA1_c30440 at 3180.029 kb on + strand, within PGA1_c30440 at 3180.082 kb on + strand, within PGA1_c30440 at 3180.090 kb on - strand, within PGA1_c30440 at 3180.090 kb on - strand, within PGA1_c30440 at 3180.262 kb on - strand, within PGA1_c30440 at 3180.266 kb on + strand, within PGA1_c30440 at 3180.277 kb on + strand, within PGA1_c30440 at 3180.327 kb on - strand, within PGA1_c30440 at 3180.355 kb on + strand, within PGA1_c30440 at 3180.355 kb on + strand, within PGA1_c30440 at 3180.361 kb on - strand, within PGA1_c30440 at 3180.363 kb on - strand, within PGA1_c30440 at 3180.363 kb on - strand, within PGA1_c30440 at 3180.363 kb on - strand, within PGA1_c30440 at 3180.363 kb on - strand, within PGA1_c30440 at 3180.363 kb on - strand, within PGA1_c30440 at 3180.363 kb on - strand, within PGA1_c30440 at 3180.379 kb on + strand, within PGA1_c30440 at 3180.408 kb on + strand, within PGA1_c30440 at 3180.416 kb on - strand, within PGA1_c30440 at 3180.426 kb on - strand, within PGA1_c30440 at 3180.476 kb on + strand, within PGA1_c30440 at 3180.486 kb on + strand, within PGA1_c30440 at 3180.549 kb on + strand, within PGA1_c30440 at 3180.554 kb on - strand, within PGA1_c30440 at 3180.557 kb on - strand, within PGA1_c30440 at 3180.574 kb on - strand at 3180.594 kb on + strand at 3180.601 kb on - strand at 3180.601 kb on - strand at 3180.636 kb on - strand at 3180.646 kb on + strand at 3180.654 kb on + strand at 3180.683 kb on - strand at 3180.730 kb on - strand at 3180.841 kb on + strand, within PGA1_c30450 at 3180.843 kb on - strand, within PGA1_c30450 at 3180.895 kb on + strand, within PGA1_c30450 at 3180.919 kb on + strand, within PGA1_c30450 at 3180.949 kb on - strand, within PGA1_c30450 at 3180.954 kb on - strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.030 kb on - strand, within PGA1_c30450 at 3181.163 kb on + strand, within PGA1_c30450 at 3181.208 kb on + strand, within PGA1_c30450 at 3181.211 kb on - strand, within PGA1_c30450 at 3181.213 kb on + strand, within PGA1_c30450 at 3181.221 kb on - strand, within PGA1_c30450 at 3181.223 kb on - strand, within PGA1_c30450 at 3181.293 kb on + strand, within PGA1_c30450 at 3181.293 kb on + strand, within PGA1_c30450 at 3181.301 kb on - strand, within PGA1_c30450 at 3181.333 kb on - strand at 3181.333 kb on - strand at 3181.349 kb on - strand at 3181.375 kb on + strand at 3181.394 kb on + strand at 3181.422 kb on + strand at 3181.427 kb on + strand at 3181.540 kb on + strand at 3181.558 kb on - strand, within PGA1_c30460 at 3181.589 kb on - strand, within PGA1_c30460 at 3181.595 kb on - strand, within PGA1_c30460 at 3181.683 kb on + strand, within PGA1_c30460 at 3181.683 kb on + strand, within PGA1_c30460 at 3181.683 kb on + strand, within PGA1_c30460 at 3181.691 kb on - strand, within PGA1_c30460 at 3181.691 kb on - strand, within PGA1_c30460 at 3181.705 kb on + strand, within PGA1_c30460 at 3181.708 kb on - strand, within PGA1_c30460 at 3181.708 kb on - strand, within PGA1_c30460 at 3181.770 kb on - strand, within PGA1_c30460 at 3181.782 kb on + strand, within PGA1_c30460 at 3181.833 kb on - strand, within PGA1_c30460 at 3181.856 kb on + strand, within PGA1_c30460 at 3181.866 kb on + strand, within PGA1_c30460 at 3181.869 kb on - strand, within PGA1_c30460 at 3181.871 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.887 kb on - strand, within PGA1_c30460 at 3181.887 kb on - strand, within PGA1_c30460 at 3181.887 kb on - strand, within PGA1_c30460 at 3181.935 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.974 kb on - strand, within PGA1_c30460 at 3181.974 kb on - strand, within PGA1_c30460 at 3181.974 kb on - strand, within PGA1_c30460 at 3182.008 kb on + strand, within PGA1_c30460 at 3182.047 kb on + strand, within PGA1_c30460 at 3182.047 kb on + strand, within PGA1_c30460 at 3182.050 kb on - strand, within PGA1_c30460 at 3182.055 kb on - strand, within PGA1_c30460 at 3182.055 kb on - strand, within PGA1_c30460 at 3182.055 kb on - strand, within PGA1_c30460 at 3182.064 kb on + strand, within PGA1_c30460 at 3182.121 kb on - strand, within PGA1_c30460 at 3182.131 kb on + strand, within PGA1_c30460 at 3182.136 kb on + strand, within PGA1_c30460 at 3182.219 kb on - strand, within PGA1_c30460 at 3182.247 kb on - strand at 3182.318 kb on + strand at 3182.339 kb on + strand at 3182.347 kb on + strand at 3182.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 3,179,733 - -2.6 3,179,767 - PGA1_c30440 0.12 -3.1 3,179,815 + PGA1_c30440 0.16 +0.0 3,179,823 - PGA1_c30440 0.17 +2.0 3,179,830 + PGA1_c30440 0.18 +2.5 3,179,838 - PGA1_c30440 0.19 +2.3 3,179,840 - PGA1_c30440 0.19 +0.7 3,179,869 - PGA1_c30440 0.22 +0.8 3,179,885 - PGA1_c30440 0.23 -0.8 3,179,887 + PGA1_c30440 0.23 +2.7 3,179,953 + PGA1_c30440 0.30 -0.1 3,180,029 + PGA1_c30440 0.37 +1.1 3,180,029 + PGA1_c30440 0.37 +3.1 3,180,082 + PGA1_c30440 0.42 +1.8 3,180,090 - PGA1_c30440 0.43 -2.0 3,180,090 - PGA1_c30440 0.43 -2.5 3,180,262 - PGA1_c30440 0.60 +2.2 3,180,266 + PGA1_c30440 0.60 +1.4 3,180,277 + PGA1_c30440 0.61 +2.6 3,180,327 - PGA1_c30440 0.66 -1.5 3,180,355 + PGA1_c30440 0.69 +1.3 3,180,355 + PGA1_c30440 0.69 +3.1 3,180,361 - PGA1_c30440 0.69 -0.3 3,180,363 - PGA1_c30440 0.70 -1.5 3,180,363 - PGA1_c30440 0.70 -2.3 3,180,363 - PGA1_c30440 0.70 -0.2 3,180,363 - PGA1_c30440 0.70 -1.5 3,180,363 - PGA1_c30440 0.70 -2.5 3,180,363 - PGA1_c30440 0.70 -1.2 3,180,379 + PGA1_c30440 0.71 -0.7 3,180,408 + PGA1_c30440 0.74 +3.4 3,180,416 - PGA1_c30440 0.75 -0.1 3,180,426 - PGA1_c30440 0.76 +1.5 3,180,476 + PGA1_c30440 0.81 -0.9 3,180,486 + PGA1_c30440 0.81 -1.9 3,180,549 + PGA1_c30440 0.88 -1.7 3,180,554 - PGA1_c30440 0.88 +1.2 3,180,557 - PGA1_c30440 0.88 +0.2 3,180,574 - +3.5 3,180,594 + -2.3 3,180,601 - -2.0 3,180,601 - +2.2 3,180,636 - -0.9 3,180,646 + -2.7 3,180,654 + -1.2 3,180,683 - +0.9 3,180,730 - -3.2 3,180,841 + PGA1_c30450 0.20 -1.9 3,180,843 - PGA1_c30450 0.20 +2.4 3,180,895 + PGA1_c30450 0.28 -3.0 3,180,919 + PGA1_c30450 0.31 -1.3 3,180,949 - PGA1_c30450 0.36 +0.7 3,180,954 - PGA1_c30450 0.37 +2.5 3,180,997 + PGA1_c30450 0.43 +0.2 3,180,997 + PGA1_c30450 0.43 +1.0 3,180,997 + PGA1_c30450 0.43 -0.6 3,180,997 + PGA1_c30450 0.43 -1.8 3,180,997 + PGA1_c30450 0.43 -0.9 3,181,005 - PGA1_c30450 0.44 -1.4 3,181,005 - PGA1_c30450 0.44 +1.4 3,181,005 - PGA1_c30450 0.44 +1.3 3,181,005 - PGA1_c30450 0.44 +2.2 3,181,005 - PGA1_c30450 0.44 -1.4 3,181,005 - PGA1_c30450 0.44 +3.5 3,181,030 - PGA1_c30450 0.48 -2.1 3,181,163 + PGA1_c30450 0.68 +3.1 3,181,208 + PGA1_c30450 0.74 -1.8 3,181,211 - PGA1_c30450 0.75 +0.7 3,181,213 + PGA1_c30450 0.75 -2.9 3,181,221 - PGA1_c30450 0.76 +3.1 3,181,223 - PGA1_c30450 0.76 -1.9 3,181,293 + PGA1_c30450 0.87 +1.5 3,181,293 + PGA1_c30450 0.87 -1.8 3,181,301 - PGA1_c30450 0.88 +2.1 3,181,333 - +3.7 3,181,333 - -2.8 3,181,349 - -0.7 3,181,375 + -0.7 3,181,394 + +1.0 3,181,422 + -0.5 3,181,427 + +0.7 3,181,540 + +2.1 3,181,558 - PGA1_c30460 0.10 +1.2 3,181,589 - PGA1_c30460 0.14 +1.5 3,181,595 - PGA1_c30460 0.15 +1.9 3,181,683 + PGA1_c30460 0.25 -0.8 3,181,683 + PGA1_c30460 0.25 -1.2 3,181,683 + PGA1_c30460 0.25 +1.1 3,181,691 - PGA1_c30460 0.26 -1.8 3,181,691 - PGA1_c30460 0.26 +0.5 3,181,705 + PGA1_c30460 0.27 +1.5 3,181,708 - PGA1_c30460 0.28 +1.3 3,181,708 - PGA1_c30460 0.28 -2.8 3,181,770 - PGA1_c30460 0.35 -0.5 3,181,782 + PGA1_c30460 0.36 +2.1 3,181,833 - PGA1_c30460 0.42 +0.8 3,181,856 + PGA1_c30460 0.45 -1.8 3,181,866 + PGA1_c30460 0.46 -1.9 3,181,869 - PGA1_c30460 0.46 +2.3 3,181,871 + PGA1_c30460 0.47 +0.3 3,181,879 + PGA1_c30460 0.48 +1.2 3,181,879 + PGA1_c30460 0.48 +0.8 3,181,879 + PGA1_c30460 0.48 -2.6 3,181,879 + PGA1_c30460 0.48 -1.5 3,181,879 + PGA1_c30460 0.48 -1.8 3,181,879 + PGA1_c30460 0.48 +5.2 3,181,879 + PGA1_c30460 0.48 +1.8 3,181,887 - PGA1_c30460 0.48 +0.9 3,181,887 - PGA1_c30460 0.48 +1.4 3,181,887 - PGA1_c30460 0.48 -2.4 3,181,935 + PGA1_c30460 0.54 -1.3 3,181,966 + PGA1_c30460 0.58 -3.0 3,181,966 + PGA1_c30460 0.58 +1.9 3,181,966 + PGA1_c30460 0.58 +2.1 3,181,966 + PGA1_c30460 0.58 -2.0 3,181,974 - PGA1_c30460 0.59 -1.2 3,181,974 - PGA1_c30460 0.59 -2.1 3,181,974 - PGA1_c30460 0.59 +0.1 3,182,008 + PGA1_c30460 0.62 +0.8 3,182,047 + PGA1_c30460 0.67 +2.4 3,182,047 + PGA1_c30460 0.67 +0.7 3,182,050 - PGA1_c30460 0.67 -2.0 3,182,055 - PGA1_c30460 0.68 +1.0 3,182,055 - PGA1_c30460 0.68 -2.5 3,182,055 - PGA1_c30460 0.68 +1.0 3,182,064 + PGA1_c30460 0.69 +0.4 3,182,121 - PGA1_c30460 0.76 -1.4 3,182,131 + PGA1_c30460 0.77 -2.9 3,182,136 + PGA1_c30460 0.77 +0.4 3,182,219 - PGA1_c30460 0.87 -1.5 3,182,247 - -3.1 3,182,318 + -1.6 3,182,339 + +2.6 3,182,347 + +4.2 3,182,347 - +2.2
Or see this region's nucleotide sequence