Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c30400 and PGA1_c30410 are separated by 103 nucleotides PGA1_c30410 and PGA1_c30420 are separated by 137 nucleotides PGA1_c30420 and PGA1_c30430 are separated by 119 nucleotides PGA1_c30430 and PGA1_c30440 are separated by 94 nucleotides
PGA1_c30400: PGA1_c30400 - alcohol dehydrogenase AlkJ, at 3,175,427 to 3,177,016
_c30400
PGA1_c30410: PGA1_c30410 - HTH-type transcriptional regulator, AsnC family, at 3,177,120 to 3,177,578
_c30410
PGA1_c30420: PGA1_c30420 - alanine dehydrogenase Ald, at 3,177,716 to 3,178,831
_c30420
PGA1_c30430: PGA1_c30430 - transcriptional regulator, TetR family, at 3,178,951 to 3,179,550
_c30430
PGA1_c30440: PGA1_c30440 - putative NADP-dependent oxidoreductase, at 3,179,645 to 3,180,676
_c30440
Position (kb)
3177
3178
3179 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 3176.768 kb on + strand, within PGA1_c30400 at 3176.775 kb on + strand, within PGA1_c30400 at 3176.776 kb on - strand, within PGA1_c30400 at 3176.777 kb on - strand, within PGA1_c30400 at 3176.806 kb on + strand, within PGA1_c30400 at 3176.810 kb on - strand, within PGA1_c30400 at 3176.814 kb on - strand, within PGA1_c30400 at 3176.816 kb on + strand, within PGA1_c30400 at 3176.817 kb on + strand, within PGA1_c30400 at 3176.825 kb on - strand, within PGA1_c30400 at 3176.829 kb on + strand, within PGA1_c30400 at 3177.094 kb on + strand at 3177.453 kb on - strand, within PGA1_c30410 at 3177.495 kb on - strand, within PGA1_c30410 at 3177.664 kb on + strand at 3177.696 kb on + strand at 3177.713 kb on - strand at 3177.713 kb on - strand at 3177.737 kb on - strand at 3177.737 kb on - strand at 3177.739 kb on + strand at 3177.747 kb on - strand at 3177.773 kb on - strand at 3177.803 kb on + strand at 3177.828 kb on + strand, within PGA1_c30420 at 3177.836 kb on - strand, within PGA1_c30420 at 3177.836 kb on - strand, within PGA1_c30420 at 3177.845 kb on + strand, within PGA1_c30420 at 3177.860 kb on + strand, within PGA1_c30420 at 3177.894 kb on + strand, within PGA1_c30420 at 3177.899 kb on + strand, within PGA1_c30420 at 3177.899 kb on + strand, within PGA1_c30420 at 3177.907 kb on - strand, within PGA1_c30420 at 3177.958 kb on + strand, within PGA1_c30420 at 3177.966 kb on - strand, within PGA1_c30420 at 3177.977 kb on + strand, within PGA1_c30420 at 3177.994 kb on - strand, within PGA1_c30420 at 3178.005 kb on - strand, within PGA1_c30420 at 3178.011 kb on - strand, within PGA1_c30420 at 3178.027 kb on - strand, within PGA1_c30420 at 3178.152 kb on + strand, within PGA1_c30420 at 3178.152 kb on + strand, within PGA1_c30420 at 3178.152 kb on + strand, within PGA1_c30420 at 3178.152 kb on + strand, within PGA1_c30420 at 3178.152 kb on + strand, within PGA1_c30420 at 3178.152 kb on + strand, within PGA1_c30420 at 3178.160 kb on - strand, within PGA1_c30420 at 3178.176 kb on - strand, within PGA1_c30420 at 3178.187 kb on + strand, within PGA1_c30420 at 3178.217 kb on - strand, within PGA1_c30420 at 3178.265 kb on + strand, within PGA1_c30420 at 3178.389 kb on + strand, within PGA1_c30420 at 3178.389 kb on + strand, within PGA1_c30420 at 3178.389 kb on + strand, within PGA1_c30420 at 3178.397 kb on - strand, within PGA1_c30420 at 3178.397 kb on - strand, within PGA1_c30420 at 3178.438 kb on - strand, within PGA1_c30420 at 3178.438 kb on - strand, within PGA1_c30420 at 3178.438 kb on - strand, within PGA1_c30420 at 3178.438 kb on - strand, within PGA1_c30420 at 3178.494 kb on - strand, within PGA1_c30420 at 3178.510 kb on - strand, within PGA1_c30420 at 3178.510 kb on - strand, within PGA1_c30420 at 3178.545 kb on + strand, within PGA1_c30420 at 3178.582 kb on + strand, within PGA1_c30420 at 3178.600 kb on + strand, within PGA1_c30420 at 3178.638 kb on + strand, within PGA1_c30420 at 3178.643 kb on + strand, within PGA1_c30420 at 3178.646 kb on - strand, within PGA1_c30420 at 3178.657 kb on - strand, within PGA1_c30420 at 3178.667 kb on - strand, within PGA1_c30420 at 3178.721 kb on - strand at 3178.740 kb on + strand at 3178.787 kb on + strand at 3178.788 kb on - strand at 3178.788 kb on - strand at 3178.795 kb on - strand at 3178.809 kb on + strand at 3178.862 kb on - strand at 3178.867 kb on - strand at 3178.867 kb on - strand at 3178.876 kb on + strand at 3178.876 kb on + strand at 3178.876 kb on + strand at 3178.882 kb on - strand at 3178.924 kb on + strand at 3178.965 kb on - strand at 3178.974 kb on - strand at 3178.992 kb on - strand at 3179.009 kb on - strand at 3179.038 kb on - strand, within PGA1_c30430 at 3179.057 kb on + strand, within PGA1_c30430 at 3179.067 kb on - strand, within PGA1_c30430 at 3179.073 kb on - strand, within PGA1_c30430 at 3179.122 kb on + strand, within PGA1_c30430 at 3179.122 kb on - strand, within PGA1_c30430 at 3179.143 kb on - strand, within PGA1_c30430 at 3179.145 kb on + strand, within PGA1_c30430 at 3179.150 kb on + strand, within PGA1_c30430 at 3179.150 kb on + strand, within PGA1_c30430 at 3179.158 kb on - strand, within PGA1_c30430 at 3179.198 kb on + strand, within PGA1_c30430 at 3179.198 kb on + strand, within PGA1_c30430 at 3179.203 kb on + strand, within PGA1_c30430 at 3179.216 kb on - strand, within PGA1_c30430 at 3179.248 kb on - strand, within PGA1_c30430 at 3179.248 kb on - strand, within PGA1_c30430 at 3179.308 kb on + strand, within PGA1_c30430 at 3179.309 kb on - strand, within PGA1_c30430 at 3179.333 kb on - strand, within PGA1_c30430 at 3179.370 kb on + strand, within PGA1_c30430 at 3179.370 kb on + strand, within PGA1_c30430 at 3179.370 kb on + strand, within PGA1_c30430 at 3179.378 kb on - strand, within PGA1_c30430 at 3179.415 kb on + strand, within PGA1_c30430 at 3179.423 kb on - strand, within PGA1_c30430 at 3179.423 kb on - strand, within PGA1_c30430 at 3179.461 kb on - strand, within PGA1_c30430 at 3179.481 kb on + strand, within PGA1_c30430 at 3179.483 kb on + strand, within PGA1_c30430 at 3179.509 kb on + strand at 3179.514 kb on + strand at 3179.522 kb on - strand at 3179.553 kb on + strand at 3179.553 kb on + strand at 3179.624 kb on - strand at 3179.624 kb on - strand at 3179.692 kb on + strand at 3179.692 kb on + strand at 3179.692 kb on + strand at 3179.692 kb on + strand at 3179.692 kb on + strand at 3179.700 kb on - strand at 3179.700 kb on - strand at 3179.700 kb on - strand at 3179.700 kb on - strand at 3179.700 kb on - strand at 3179.700 kb on - strand at 3179.733 kb on - strand at 3179.767 kb on - strand, within PGA1_c30440 at 3179.815 kb on + strand, within PGA1_c30440 at 3179.823 kb on - strand, within PGA1_c30440 at 3179.830 kb on + strand, within PGA1_c30440
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 3,176,768 + PGA1_c30400 0.84 +2.3 3,176,775 + PGA1_c30400 0.85 +0.1 3,176,776 - PGA1_c30400 0.85 -3.1 3,176,777 - PGA1_c30400 0.85 +1.0 3,176,806 + PGA1_c30400 0.87 -2.7 3,176,810 - PGA1_c30400 0.87 +2.1 3,176,814 - PGA1_c30400 0.87 +0.1 3,176,816 + PGA1_c30400 0.87 +2.6 3,176,817 + PGA1_c30400 0.87 +3.1 3,176,825 - PGA1_c30400 0.88 -2.4 3,176,829 + PGA1_c30400 0.88 +0.9 3,177,094 + +0.9 3,177,453 - PGA1_c30410 0.73 -1.3 3,177,495 - PGA1_c30410 0.82 +0.2 3,177,664 + -0.5 3,177,696 + -1.4 3,177,713 - -1.2 3,177,713 - -1.4 3,177,737 - -0.4 3,177,737 - -0.3 3,177,739 + -3.0 3,177,747 - -1.2 3,177,773 - +1.3 3,177,803 + -2.1 3,177,828 + PGA1_c30420 0.10 +1.4 3,177,836 - PGA1_c30420 0.11 +2.0 3,177,836 - PGA1_c30420 0.11 +2.2 3,177,845 + PGA1_c30420 0.12 +1.2 3,177,860 + PGA1_c30420 0.13 +2.9 3,177,894 + PGA1_c30420 0.16 -0.4 3,177,899 + PGA1_c30420 0.16 -0.3 3,177,899 + PGA1_c30420 0.16 -0.8 3,177,907 - PGA1_c30420 0.17 +1.8 3,177,958 + PGA1_c30420 0.22 -1.5 3,177,966 - PGA1_c30420 0.22 +2.1 3,177,977 + PGA1_c30420 0.23 -1.4 3,177,994 - PGA1_c30420 0.25 -1.6 3,178,005 - PGA1_c30420 0.26 -2.2 3,178,011 - PGA1_c30420 0.26 +2.4 3,178,027 - PGA1_c30420 0.28 +0.6 3,178,152 + PGA1_c30420 0.39 -2.1 3,178,152 + PGA1_c30420 0.39 +0.9 3,178,152 + PGA1_c30420 0.39 +2.1 3,178,152 + PGA1_c30420 0.39 -1.7 3,178,152 + PGA1_c30420 0.39 +1.7 3,178,152 + PGA1_c30420 0.39 +0.1 3,178,160 - PGA1_c30420 0.40 +0.6 3,178,176 - PGA1_c30420 0.41 +4.0 3,178,187 + PGA1_c30420 0.42 -1.2 3,178,217 - PGA1_c30420 0.45 -2.5 3,178,265 + PGA1_c30420 0.49 +2.5 3,178,389 + PGA1_c30420 0.60 -1.1 3,178,389 + PGA1_c30420 0.60 -0.6 3,178,389 + PGA1_c30420 0.60 -0.3 3,178,397 - PGA1_c30420 0.61 -2.0 3,178,397 - PGA1_c30420 0.61 -0.9 3,178,438 - PGA1_c30420 0.65 +2.6 3,178,438 - PGA1_c30420 0.65 +2.9 3,178,438 - PGA1_c30420 0.65 +2.1 3,178,438 - PGA1_c30420 0.65 +2.2 3,178,494 - PGA1_c30420 0.70 -1.7 3,178,510 - PGA1_c30420 0.71 -1.5 3,178,510 - PGA1_c30420 0.71 -1.3 3,178,545 + PGA1_c30420 0.74 +2.2 3,178,582 + PGA1_c30420 0.78 -1.6 3,178,600 + PGA1_c30420 0.79 -0.7 3,178,638 + PGA1_c30420 0.83 +3.0 3,178,643 + PGA1_c30420 0.83 -0.5 3,178,646 - PGA1_c30420 0.83 -2.4 3,178,657 - PGA1_c30420 0.84 -1.5 3,178,667 - PGA1_c30420 0.85 -1.7 3,178,721 - +1.0 3,178,740 + +0.7 3,178,787 + +2.7 3,178,788 - -2.3 3,178,788 - -2.7 3,178,795 - +0.7 3,178,809 + -2.4 3,178,862 - +1.9 3,178,867 - -2.4 3,178,867 - +1.0 3,178,876 + +3.1 3,178,876 + -1.4 3,178,876 + +0.4 3,178,882 - -2.4 3,178,924 + +1.9 3,178,965 - +2.7 3,178,974 - +2.3 3,178,992 - +2.8 3,179,009 - +0.6 3,179,038 - PGA1_c30430 0.14 -2.5 3,179,057 + PGA1_c30430 0.18 -1.8 3,179,067 - PGA1_c30430 0.19 +1.5 3,179,073 - PGA1_c30430 0.20 -1.6 3,179,122 + PGA1_c30430 0.28 +2.2 3,179,122 - PGA1_c30430 0.28 -2.4 3,179,143 - PGA1_c30430 0.32 -0.8 3,179,145 + PGA1_c30430 0.32 -1.6 3,179,150 + PGA1_c30430 0.33 -1.1 3,179,150 + PGA1_c30430 0.33 -2.1 3,179,158 - PGA1_c30430 0.34 +1.1 3,179,198 + PGA1_c30430 0.41 +2.4 3,179,198 + PGA1_c30430 0.41 +0.8 3,179,203 + PGA1_c30430 0.42 -0.5 3,179,216 - PGA1_c30430 0.44 -1.0 3,179,248 - PGA1_c30430 0.49 -2.7 3,179,248 - PGA1_c30430 0.49 +4.3 3,179,308 + PGA1_c30430 0.59 +2.1 3,179,309 - PGA1_c30430 0.60 -2.4 3,179,333 - PGA1_c30430 0.64 +2.8 3,179,370 + PGA1_c30430 0.70 -2.4 3,179,370 + PGA1_c30430 0.70 +2.3 3,179,370 + PGA1_c30430 0.70 +2.2 3,179,378 - PGA1_c30430 0.71 +0.4 3,179,415 + PGA1_c30430 0.77 -0.9 3,179,423 - PGA1_c30430 0.79 +2.6 3,179,423 - PGA1_c30430 0.79 +1.4 3,179,461 - PGA1_c30430 0.85 +0.9 3,179,481 + PGA1_c30430 0.88 -3.0 3,179,483 + PGA1_c30430 0.89 +1.5 3,179,509 + +0.8 3,179,514 + +1.2 3,179,522 - -2.1 3,179,553 + +0.9 3,179,553 + +5.0 3,179,624 - -1.3 3,179,624 - -1.8 3,179,692 + -1.3 3,179,692 + -0.8 3,179,692 + -0.4 3,179,692 + +3.0 3,179,692 + -1.7 3,179,700 - -1.3 3,179,700 - -1.3 3,179,700 - -2.6 3,179,700 - -0.6 3,179,700 - -0.3 3,179,700 - +1.7 3,179,733 - -2.6 3,179,767 - PGA1_c30440 0.12 -3.1 3,179,815 + PGA1_c30440 0.16 +0.0 3,179,823 - PGA1_c30440 0.17 +2.0 3,179,830 + PGA1_c30440 0.18 +2.5
Or see this region's nucleotide sequence