Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c30390 and PGA1_c30400 are separated by 218 nucleotides PGA1_c30400 and PGA1_c30410 are separated by 103 nucleotides PGA1_c30410 and PGA1_c30420 are separated by 137 nucleotides
PGA1_c30390: PGA1_c30390 - putative polyphosphate kinase, at 3,174,291 to 3,175,208
_c30390
PGA1_c30400: PGA1_c30400 - alcohol dehydrogenase AlkJ, at 3,175,427 to 3,177,016
_c30400
PGA1_c30410: PGA1_c30410 - HTH-type transcriptional regulator, AsnC family, at 3,177,120 to 3,177,578
_c30410
PGA1_c30420: PGA1_c30420 - alanine dehydrogenase Ald, at 3,177,716 to 3,178,831
_c30420
Position (kb)
3175
3176
3177
3178 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3174.427 kb on - strand, within PGA1_c30390 at 3174.427 kb on - strand, within PGA1_c30390 at 3174.427 kb on - strand, within PGA1_c30390 at 3174.432 kb on - strand, within PGA1_c30390 at 3174.450 kb on - strand, within PGA1_c30390 at 3174.472 kb on - strand, within PGA1_c30390 at 3174.510 kb on - strand, within PGA1_c30390 at 3174.587 kb on + strand, within PGA1_c30390 at 3174.587 kb on + strand, within PGA1_c30390 at 3174.664 kb on - strand, within PGA1_c30390 at 3174.711 kb on + strand, within PGA1_c30390 at 3174.859 kb on - strand, within PGA1_c30390 at 3174.870 kb on - strand, within PGA1_c30390 at 3174.882 kb on - strand, within PGA1_c30390 at 3174.896 kb on + strand, within PGA1_c30390 at 3174.953 kb on - strand, within PGA1_c30390 at 3174.953 kb on - strand, within PGA1_c30390 at 3174.971 kb on + strand, within PGA1_c30390 at 3174.984 kb on + strand, within PGA1_c30390 at 3175.064 kb on - strand, within PGA1_c30390 at 3175.072 kb on - strand, within PGA1_c30390 at 3175.150 kb on - strand at 3175.152 kb on + strand at 3175.160 kb on - strand at 3175.160 kb on - strand at 3175.160 kb on - strand at 3175.203 kb on + strand at 3175.208 kb on + strand at 3175.208 kb on + strand at 3175.211 kb on - strand at 3175.216 kb on - strand at 3175.216 kb on - strand at 3175.216 kb on - strand at 3175.216 kb on - strand at 3175.216 kb on - strand at 3175.234 kb on - strand at 3175.324 kb on + strand at 3175.329 kb on - strand at 3175.400 kb on - strand at 3175.405 kb on + strand at 3175.537 kb on + strand at 3175.591 kb on + strand, within PGA1_c30400 at 3175.609 kb on + strand, within PGA1_c30400 at 3175.658 kb on + strand, within PGA1_c30400 at 3175.683 kb on - strand, within PGA1_c30400 at 3175.734 kb on - strand, within PGA1_c30400 at 3175.760 kb on - strand, within PGA1_c30400 at 3175.810 kb on - strand, within PGA1_c30400 at 3175.836 kb on + strand, within PGA1_c30400 at 3175.839 kb on - strand, within PGA1_c30400 at 3175.887 kb on + strand, within PGA1_c30400 at 3175.938 kb on + strand, within PGA1_c30400 at 3175.944 kb on - strand, within PGA1_c30400 at 3175.946 kb on - strand, within PGA1_c30400 at 3175.948 kb on + strand, within PGA1_c30400 at 3175.987 kb on - strand, within PGA1_c30400 at 3176.045 kb on - strand, within PGA1_c30400 at 3176.107 kb on + strand, within PGA1_c30400 at 3176.115 kb on - strand, within PGA1_c30400 at 3176.115 kb on - strand, within PGA1_c30400 at 3176.115 kb on - strand, within PGA1_c30400 at 3176.150 kb on + strand, within PGA1_c30400 at 3176.158 kb on - strand, within PGA1_c30400 at 3176.159 kb on + strand, within PGA1_c30400 at 3176.167 kb on - strand, within PGA1_c30400 at 3176.178 kb on - strand, within PGA1_c30400 at 3176.191 kb on - strand, within PGA1_c30400 at 3176.199 kb on - strand, within PGA1_c30400 at 3176.199 kb on - strand, within PGA1_c30400 at 3176.220 kb on + strand, within PGA1_c30400 at 3176.229 kb on + strand, within PGA1_c30400 at 3176.233 kb on - strand, within PGA1_c30400 at 3176.233 kb on - strand, within PGA1_c30400 at 3176.238 kb on - strand, within PGA1_c30400 at 3176.266 kb on - strand, within PGA1_c30400 at 3176.266 kb on - strand, within PGA1_c30400 at 3176.304 kb on + strand, within PGA1_c30400 at 3176.312 kb on - strand, within PGA1_c30400 at 3176.321 kb on - strand, within PGA1_c30400 at 3176.323 kb on + strand, within PGA1_c30400 at 3176.393 kb on - strand, within PGA1_c30400 at 3176.444 kb on - strand, within PGA1_c30400 at 3176.456 kb on + strand, within PGA1_c30400 at 3176.492 kb on - strand, within PGA1_c30400 at 3176.492 kb on - strand, within PGA1_c30400 at 3176.514 kb on + strand, within PGA1_c30400 at 3176.545 kb on + strand, within PGA1_c30400 at 3176.558 kb on - strand, within PGA1_c30400 at 3176.560 kb on + strand, within PGA1_c30400 at 3176.565 kb on + strand, within PGA1_c30400 at 3176.573 kb on - strand, within PGA1_c30400 at 3176.617 kb on + strand, within PGA1_c30400 at 3176.617 kb on + strand, within PGA1_c30400 at 3176.617 kb on + strand, within PGA1_c30400 at 3176.617 kb on + strand, within PGA1_c30400 at 3176.617 kb on + strand, within PGA1_c30400 at 3176.617 kb on + strand, within PGA1_c30400 at 3176.625 kb on - strand, within PGA1_c30400 at 3176.648 kb on - strand, within PGA1_c30400 at 3176.658 kb on - strand, within PGA1_c30400 at 3176.676 kb on - strand, within PGA1_c30400 at 3176.676 kb on - strand, within PGA1_c30400 at 3176.694 kb on - strand, within PGA1_c30400 at 3176.768 kb on + strand, within PGA1_c30400 at 3176.775 kb on + strand, within PGA1_c30400 at 3176.776 kb on - strand, within PGA1_c30400 at 3176.777 kb on - strand, within PGA1_c30400 at 3176.806 kb on + strand, within PGA1_c30400 at 3176.810 kb on - strand, within PGA1_c30400 at 3176.814 kb on - strand, within PGA1_c30400 at 3176.816 kb on + strand, within PGA1_c30400 at 3176.817 kb on + strand, within PGA1_c30400 at 3176.825 kb on - strand, within PGA1_c30400 at 3176.829 kb on + strand, within PGA1_c30400 at 3177.094 kb on + strand at 3177.453 kb on - strand, within PGA1_c30410 at 3177.495 kb on - strand, within PGA1_c30410 at 3177.664 kb on + strand at 3177.696 kb on + strand at 3177.713 kb on - strand at 3177.713 kb on - strand at 3177.737 kb on - strand at 3177.737 kb on - strand at 3177.739 kb on + strand at 3177.747 kb on - strand at 3177.773 kb on - strand at 3177.803 kb on + strand at 3177.828 kb on + strand, within PGA1_c30420 at 3177.836 kb on - strand, within PGA1_c30420 at 3177.836 kb on - strand, within PGA1_c30420 at 3177.845 kb on + strand, within PGA1_c30420 at 3177.860 kb on + strand, within PGA1_c30420 at 3177.894 kb on + strand, within PGA1_c30420 at 3177.899 kb on + strand, within PGA1_c30420 at 3177.899 kb on + strand, within PGA1_c30420 at 3177.907 kb on - strand, within PGA1_c30420 at 3177.958 kb on + strand, within PGA1_c30420 at 3177.966 kb on - strand, within PGA1_c30420 at 3177.977 kb on + strand, within PGA1_c30420 at 3177.994 kb on - strand, within PGA1_c30420 at 3178.005 kb on - strand, within PGA1_c30420 at 3178.011 kb on - strand, within PGA1_c30420
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 3,174,427 - PGA1_c30390 0.15 +0.1 3,174,427 - PGA1_c30390 0.15 -1.7 3,174,427 - PGA1_c30390 0.15 -1.5 3,174,432 - PGA1_c30390 0.15 -2.7 3,174,450 - PGA1_c30390 0.17 -3.2 3,174,472 - PGA1_c30390 0.20 -1.8 3,174,510 - PGA1_c30390 0.24 -1.4 3,174,587 + PGA1_c30390 0.32 -2.5 3,174,587 + PGA1_c30390 0.32 -1.6 3,174,664 - PGA1_c30390 0.41 -2.1 3,174,711 + PGA1_c30390 0.46 -2.6 3,174,859 - PGA1_c30390 0.62 -1.3 3,174,870 - PGA1_c30390 0.63 -2.4 3,174,882 - PGA1_c30390 0.64 -1.5 3,174,896 + PGA1_c30390 0.66 -1.0 3,174,953 - PGA1_c30390 0.72 -2.4 3,174,953 - PGA1_c30390 0.72 -0.9 3,174,971 + PGA1_c30390 0.74 +0.3 3,174,984 + PGA1_c30390 0.75 -1.2 3,175,064 - PGA1_c30390 0.84 -0.1 3,175,072 - PGA1_c30390 0.85 -3.3 3,175,150 - -2.7 3,175,152 + -2.5 3,175,160 - -1.6 3,175,160 - -3.0 3,175,160 - +0.3 3,175,203 + -1.9 3,175,208 + -0.3 3,175,208 + +3.9 3,175,211 - +0.7 3,175,216 - +3.4 3,175,216 - -2.0 3,175,216 - -1.6 3,175,216 - -0.7 3,175,216 - -0.8 3,175,234 - -1.5 3,175,324 + -0.4 3,175,329 - -0.6 3,175,400 - +1.7 3,175,405 + -3.1 3,175,537 + +1.2 3,175,591 + PGA1_c30400 0.10 -1.5 3,175,609 + PGA1_c30400 0.11 +1.8 3,175,658 + PGA1_c30400 0.15 -0.9 3,175,683 - PGA1_c30400 0.16 +3.7 3,175,734 - PGA1_c30400 0.19 +1.2 3,175,760 - PGA1_c30400 0.21 -1.4 3,175,810 - PGA1_c30400 0.24 +2.5 3,175,836 + PGA1_c30400 0.26 -0.1 3,175,839 - PGA1_c30400 0.26 +2.4 3,175,887 + PGA1_c30400 0.29 -4.6 3,175,938 + PGA1_c30400 0.32 +0.3 3,175,944 - PGA1_c30400 0.33 -2.5 3,175,946 - PGA1_c30400 0.33 -2.1 3,175,948 + PGA1_c30400 0.33 -1.3 3,175,987 - PGA1_c30400 0.35 -0.7 3,176,045 - PGA1_c30400 0.39 -0.1 3,176,107 + PGA1_c30400 0.43 +2.3 3,176,115 - PGA1_c30400 0.43 -1.1 3,176,115 - PGA1_c30400 0.43 -2.4 3,176,115 - PGA1_c30400 0.43 -3.5 3,176,150 + PGA1_c30400 0.45 +1.8 3,176,158 - PGA1_c30400 0.46 +0.7 3,176,159 + PGA1_c30400 0.46 -1.6 3,176,167 - PGA1_c30400 0.47 +3.2 3,176,178 - PGA1_c30400 0.47 +2.3 3,176,191 - PGA1_c30400 0.48 +1.6 3,176,199 - PGA1_c30400 0.49 +1.4 3,176,199 - PGA1_c30400 0.49 -0.6 3,176,220 + PGA1_c30400 0.50 -3.4 3,176,229 + PGA1_c30400 0.50 +0.7 3,176,233 - PGA1_c30400 0.51 +2.1 3,176,233 - PGA1_c30400 0.51 +0.3 3,176,238 - PGA1_c30400 0.51 -2.4 3,176,266 - PGA1_c30400 0.53 -1.1 3,176,266 - PGA1_c30400 0.53 -0.5 3,176,304 + PGA1_c30400 0.55 +1.8 3,176,312 - PGA1_c30400 0.56 -0.8 3,176,321 - PGA1_c30400 0.56 -2.4 3,176,323 + PGA1_c30400 0.56 -0.8 3,176,393 - PGA1_c30400 0.61 -2.5 3,176,444 - PGA1_c30400 0.64 -1.4 3,176,456 + PGA1_c30400 0.65 +0.3 3,176,492 - PGA1_c30400 0.67 +1.1 3,176,492 - PGA1_c30400 0.67 -0.7 3,176,514 + PGA1_c30400 0.68 -1.4 3,176,545 + PGA1_c30400 0.70 +0.1 3,176,558 - PGA1_c30400 0.71 +1.8 3,176,560 + PGA1_c30400 0.71 -0.7 3,176,565 + PGA1_c30400 0.72 -1.6 3,176,573 - PGA1_c30400 0.72 -1.0 3,176,617 + PGA1_c30400 0.75 -1.5 3,176,617 + PGA1_c30400 0.75 -1.6 3,176,617 + PGA1_c30400 0.75 -1.6 3,176,617 + PGA1_c30400 0.75 +3.0 3,176,617 + PGA1_c30400 0.75 +1.0 3,176,617 + PGA1_c30400 0.75 +1.5 3,176,625 - PGA1_c30400 0.75 -1.4 3,176,648 - PGA1_c30400 0.77 -1.8 3,176,658 - PGA1_c30400 0.77 +2.1 3,176,676 - PGA1_c30400 0.79 -1.2 3,176,676 - PGA1_c30400 0.79 +2.8 3,176,694 - PGA1_c30400 0.80 +0.5 3,176,768 + PGA1_c30400 0.84 +2.3 3,176,775 + PGA1_c30400 0.85 +0.1 3,176,776 - PGA1_c30400 0.85 -3.1 3,176,777 - PGA1_c30400 0.85 +1.0 3,176,806 + PGA1_c30400 0.87 -2.7 3,176,810 - PGA1_c30400 0.87 +2.1 3,176,814 - PGA1_c30400 0.87 +0.1 3,176,816 + PGA1_c30400 0.87 +2.6 3,176,817 + PGA1_c30400 0.87 +3.1 3,176,825 - PGA1_c30400 0.88 -2.4 3,176,829 + PGA1_c30400 0.88 +0.9 3,177,094 + +0.9 3,177,453 - PGA1_c30410 0.73 -1.3 3,177,495 - PGA1_c30410 0.82 +0.2 3,177,664 + -0.5 3,177,696 + -1.4 3,177,713 - -1.2 3,177,713 - -1.4 3,177,737 - -0.4 3,177,737 - -0.3 3,177,739 + -3.0 3,177,747 - -1.2 3,177,773 - +1.3 3,177,803 + -2.1 3,177,828 + PGA1_c30420 0.10 +1.4 3,177,836 - PGA1_c30420 0.11 +2.0 3,177,836 - PGA1_c30420 0.11 +2.2 3,177,845 + PGA1_c30420 0.12 +1.2 3,177,860 + PGA1_c30420 0.13 +2.9 3,177,894 + PGA1_c30420 0.16 -0.4 3,177,899 + PGA1_c30420 0.16 -0.3 3,177,899 + PGA1_c30420 0.16 -0.8 3,177,907 - PGA1_c30420 0.17 +1.8 3,177,958 + PGA1_c30420 0.22 -1.5 3,177,966 - PGA1_c30420 0.22 +2.1 3,177,977 + PGA1_c30420 0.23 -1.4 3,177,994 - PGA1_c30420 0.25 -1.6 3,178,005 - PGA1_c30420 0.26 -2.2 3,178,011 - PGA1_c30420 0.26 +2.4
Or see this region's nucleotide sequence