Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03090 and PGA1_c03100 are separated by 5 nucleotides PGA1_c03100 and PGA1_c03110 are separated by 33 nucleotides PGA1_c03110 and PGA1_c03120 are separated by 32 nucleotides
PGA1_c03090: PGA1_c03090 - putative secretion protein, at 295,424 to 296,914
_c03090
PGA1_c03100: PGA1_c03100 - putative aminoglycoside efflux pump, at 296,920 to 300,315
_c03100
PGA1_c03110: PGA1_c03110 - NUDIX hydrolase, at 300,349 to 300,858
_c03110
PGA1_c03120: PGA1_c03120 - hypothetical protein, at 300,891 to 301,745
_c03120
Position (kb) 296
297
298
299
300
301 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 295.943 kb on - strand, within PGA1_c03090 at 295.987 kb on - strand, within PGA1_c03090 at 296.010 kb on + strand, within PGA1_c03090 at 296.020 kb on + strand, within PGA1_c03090 at 296.083 kb on + strand, within PGA1_c03090 at 296.083 kb on + strand, within PGA1_c03090 at 296.113 kb on + strand, within PGA1_c03090 at 296.121 kb on + strand, within PGA1_c03090 at 296.181 kb on - strand, within PGA1_c03090 at 296.230 kb on - strand, within PGA1_c03090 at 296.271 kb on + strand, within PGA1_c03090 at 296.279 kb on - strand, within PGA1_c03090 at 296.372 kb on + strand, within PGA1_c03090 at 296.473 kb on - strand, within PGA1_c03090 at 296.516 kb on - strand, within PGA1_c03090 at 296.610 kb on - strand, within PGA1_c03090 at 296.612 kb on + strand, within PGA1_c03090 at 296.613 kb on + strand, within PGA1_c03090 at 296.620 kb on - strand, within PGA1_c03090 at 296.620 kb on - strand, within PGA1_c03090 at 296.638 kb on - strand, within PGA1_c03090 at 296.646 kb on + strand, within PGA1_c03090 at 296.646 kb on + strand, within PGA1_c03090 at 296.654 kb on - strand, within PGA1_c03090 at 296.691 kb on + strand, within PGA1_c03090 at 296.691 kb on + strand, within PGA1_c03090 at 296.691 kb on + strand, within PGA1_c03090 at 296.691 kb on + strand, within PGA1_c03090 at 296.923 kb on - strand at 296.923 kb on - strand at 297.118 kb on + strand at 297.118 kb on - strand at 297.118 kb on - strand at 297.188 kb on + strand at 297.196 kb on - strand at 297.232 kb on + strand at 297.327 kb on + strand, within PGA1_c03100 at 297.392 kb on + strand, within PGA1_c03100 at 297.392 kb on + strand, within PGA1_c03100 at 297.428 kb on + strand, within PGA1_c03100 at 297.585 kb on + strand, within PGA1_c03100 at 297.632 kb on + strand, within PGA1_c03100 at 297.682 kb on + strand, within PGA1_c03100 at 297.685 kb on - strand, within PGA1_c03100 at 297.685 kb on - strand, within PGA1_c03100 at 297.691 kb on + strand, within PGA1_c03100 at 297.790 kb on + strand, within PGA1_c03100 at 297.798 kb on - strand, within PGA1_c03100 at 297.830 kb on - strand, within PGA1_c03100 at 297.834 kb on + strand, within PGA1_c03100 at 297.877 kb on + strand, within PGA1_c03100 at 297.890 kb on - strand, within PGA1_c03100 at 297.937 kb on + strand, within PGA1_c03100 at 297.961 kb on - strand, within PGA1_c03100 at 298.101 kb on - strand, within PGA1_c03100 at 298.106 kb on + strand, within PGA1_c03100 at 298.272 kb on + strand, within PGA1_c03100 at 298.395 kb on + strand, within PGA1_c03100 at 298.411 kb on - strand, within PGA1_c03100 at 298.411 kb on - strand, within PGA1_c03100 at 298.413 kb on + strand, within PGA1_c03100 at 298.418 kb on + strand, within PGA1_c03100 at 298.418 kb on + strand, within PGA1_c03100 at 298.458 kb on - strand, within PGA1_c03100 at 298.462 kb on + strand, within PGA1_c03100 at 298.517 kb on - strand, within PGA1_c03100 at 298.522 kb on - strand, within PGA1_c03100 at 298.561 kb on - strand, within PGA1_c03100 at 298.713 kb on + strand, within PGA1_c03100 at 298.737 kb on + strand, within PGA1_c03100 at 298.786 kb on + strand, within PGA1_c03100 at 298.791 kb on + strand, within PGA1_c03100 at 298.799 kb on - strand, within PGA1_c03100 at 298.881 kb on - strand, within PGA1_c03100 at 298.902 kb on - strand, within PGA1_c03100 at 298.981 kb on - strand, within PGA1_c03100 at 298.986 kb on + strand, within PGA1_c03100 at 298.986 kb on + strand, within PGA1_c03100 at 299.018 kb on - strand, within PGA1_c03100 at 299.018 kb on - strand, within PGA1_c03100 at 299.044 kb on + strand, within PGA1_c03100 at 299.044 kb on + strand, within PGA1_c03100 at 299.054 kb on + strand, within PGA1_c03100 at 299.054 kb on + strand, within PGA1_c03100 at 299.054 kb on + strand, within PGA1_c03100 at 299.054 kb on + strand, within PGA1_c03100 at 299.054 kb on + strand, within PGA1_c03100 at 299.062 kb on - strand, within PGA1_c03100 at 299.062 kb on - strand, within PGA1_c03100 at 299.062 kb on - strand, within PGA1_c03100 at 299.197 kb on - strand, within PGA1_c03100 at 299.289 kb on + strand, within PGA1_c03100 at 299.305 kb on - strand, within PGA1_c03100 at 299.311 kb on + strand, within PGA1_c03100 at 299.419 kb on - strand, within PGA1_c03100 at 299.516 kb on + strand, within PGA1_c03100 at 299.595 kb on - strand, within PGA1_c03100 at 299.623 kb on - strand, within PGA1_c03100 at 299.627 kb on + strand, within PGA1_c03100 at 299.678 kb on - strand, within PGA1_c03100 at 299.693 kb on - strand, within PGA1_c03100 at 299.886 kb on + strand, within PGA1_c03100 at 299.895 kb on + strand, within PGA1_c03100 at 299.949 kb on + strand, within PGA1_c03100 at 299.957 kb on - strand, within PGA1_c03100 at 300.019 kb on + strand at 300.019 kb on + strand at 300.089 kb on + strand at 300.205 kb on + strand at 300.215 kb on + strand at 300.319 kb on + strand at 300.425 kb on - strand, within PGA1_c03110 at 300.430 kb on - strand, within PGA1_c03110 at 300.539 kb on + strand, within PGA1_c03110 at 300.612 kb on + strand, within PGA1_c03110 at 300.620 kb on - strand, within PGA1_c03110 at 300.669 kb on - strand, within PGA1_c03110 at 300.707 kb on - strand, within PGA1_c03110 at 300.713 kb on + strand, within PGA1_c03110 at 300.743 kb on - strand, within PGA1_c03110 at 300.859 kb on + strand at 300.864 kb on + strand at 300.906 kb on + strand at 300.906 kb on + strand at 300.976 kb on + strand at 300.976 kb on + strand at 300.984 kb on - strand, within PGA1_c03120 at 301.036 kb on + strand, within PGA1_c03120 at 301.110 kb on - strand, within PGA1_c03120 at 301.172 kb on - strand, within PGA1_c03120 at 301.274 kb on + strand, within PGA1_c03120 at 301.282 kb on + strand, within PGA1_c03120 at 301.282 kb on - strand, within PGA1_c03120 at 301.302 kb on - strand, within PGA1_c03120
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 295,943 - PGA1_c03090 0.35 +0.1 295,987 - PGA1_c03090 0.38 -2.4 296,010 + PGA1_c03090 0.39 +1.5 296,020 + PGA1_c03090 0.40 -1.6 296,083 + PGA1_c03090 0.44 +0.0 296,083 + PGA1_c03090 0.44 +2.3 296,113 + PGA1_c03090 0.46 -2.2 296,121 + PGA1_c03090 0.47 -1.2 296,181 - PGA1_c03090 0.51 -1.2 296,230 - PGA1_c03090 0.54 +0.3 296,271 + PGA1_c03090 0.57 +0.1 296,279 - PGA1_c03090 0.57 +2.8 296,372 + PGA1_c03090 0.64 -2.6 296,473 - PGA1_c03090 0.70 -1.2 296,516 - PGA1_c03090 0.73 -2.5 296,610 - PGA1_c03090 0.80 -1.1 296,612 + PGA1_c03090 0.80 +0.8 296,613 + PGA1_c03090 0.80 +1.9 296,620 - PGA1_c03090 0.80 -1.9 296,620 - PGA1_c03090 0.80 -1.3 296,638 - PGA1_c03090 0.81 -3.2 296,646 + PGA1_c03090 0.82 +2.1 296,646 + PGA1_c03090 0.82 -2.3 296,654 - PGA1_c03090 0.82 +1.0 296,691 + PGA1_c03090 0.85 -0.9 296,691 + PGA1_c03090 0.85 +1.1 296,691 + PGA1_c03090 0.85 -0.2 296,691 + PGA1_c03090 0.85 +1.5 296,923 - +2.5 296,923 - -2.4 297,118 + -0.0 297,118 - +2.0 297,118 - -1.6 297,188 + +0.8 297,196 - +2.6 297,232 + -0.1 297,327 + PGA1_c03100 0.12 -2.8 297,392 + PGA1_c03100 0.14 -0.1 297,392 + PGA1_c03100 0.14 +1.7 297,428 + PGA1_c03100 0.15 -0.9 297,585 + PGA1_c03100 0.20 -2.7 297,632 + PGA1_c03100 0.21 -1.5 297,682 + PGA1_c03100 0.22 -0.6 297,685 - PGA1_c03100 0.23 -1.7 297,685 - PGA1_c03100 0.23 -0.7 297,691 + PGA1_c03100 0.23 -2.5 297,790 + PGA1_c03100 0.26 +2.0 297,798 - PGA1_c03100 0.26 -2.4 297,830 - PGA1_c03100 0.27 +2.8 297,834 + PGA1_c03100 0.27 +3.2 297,877 + PGA1_c03100 0.28 +3.5 297,890 - PGA1_c03100 0.29 -0.9 297,937 + PGA1_c03100 0.30 -1.8 297,961 - PGA1_c03100 0.31 -1.9 298,101 - PGA1_c03100 0.35 +0.6 298,106 + PGA1_c03100 0.35 +3.0 298,272 + PGA1_c03100 0.40 +2.0 298,395 + PGA1_c03100 0.43 -1.0 298,411 - PGA1_c03100 0.44 -1.9 298,411 - PGA1_c03100 0.44 -2.6 298,413 + PGA1_c03100 0.44 -0.9 298,418 + PGA1_c03100 0.44 +0.7 298,418 + PGA1_c03100 0.44 +0.2 298,458 - PGA1_c03100 0.45 -0.7 298,462 + PGA1_c03100 0.45 +0.1 298,517 - PGA1_c03100 0.47 -1.8 298,522 - PGA1_c03100 0.47 +2.6 298,561 - PGA1_c03100 0.48 +3.1 298,713 + PGA1_c03100 0.53 -1.3 298,737 + PGA1_c03100 0.54 -2.8 298,786 + PGA1_c03100 0.55 -3.7 298,791 + PGA1_c03100 0.55 +2.3 298,799 - PGA1_c03100 0.55 -1.4 298,881 - PGA1_c03100 0.58 +1.5 298,902 - PGA1_c03100 0.58 +1.2 298,981 - PGA1_c03100 0.61 -0.8 298,986 + PGA1_c03100 0.61 -1.5 298,986 + PGA1_c03100 0.61 -1.0 299,018 - PGA1_c03100 0.62 +2.5 299,018 - PGA1_c03100 0.62 +3.7 299,044 + PGA1_c03100 0.63 -1.1 299,044 + PGA1_c03100 0.63 +0.7 299,054 + PGA1_c03100 0.63 -2.4 299,054 + PGA1_c03100 0.63 -1.4 299,054 + PGA1_c03100 0.63 +0.8 299,054 + PGA1_c03100 0.63 -1.6 299,054 + PGA1_c03100 0.63 -1.6 299,062 - PGA1_c03100 0.63 +1.5 299,062 - PGA1_c03100 0.63 -1.8 299,062 - PGA1_c03100 0.63 -2.7 299,197 - PGA1_c03100 0.67 +1.9 299,289 + PGA1_c03100 0.70 +2.2 299,305 - PGA1_c03100 0.70 +0.8 299,311 + PGA1_c03100 0.70 +3.2 299,419 - PGA1_c03100 0.74 -1.3 299,516 + PGA1_c03100 0.76 -2.9 299,595 - PGA1_c03100 0.79 -0.6 299,623 - PGA1_c03100 0.80 +0.7 299,627 + PGA1_c03100 0.80 -1.1 299,678 - PGA1_c03100 0.81 -0.7 299,693 - PGA1_c03100 0.82 +2.4 299,886 + PGA1_c03100 0.87 -0.7 299,895 + PGA1_c03100 0.88 -0.6 299,949 + PGA1_c03100 0.89 -2.2 299,957 - PGA1_c03100 0.89 -1.6 300,019 + -0.4 300,019 + +2.2 300,089 + -2.6 300,205 + +1.9 300,215 + -1.4 300,319 + +1.4 300,425 - PGA1_c03110 0.15 +4.0 300,430 - PGA1_c03110 0.16 +3.1 300,539 + PGA1_c03110 0.37 -2.2 300,612 + PGA1_c03110 0.52 +0.3 300,620 - PGA1_c03110 0.53 +3.0 300,669 - PGA1_c03110 0.63 -1.9 300,707 - PGA1_c03110 0.70 +2.7 300,713 + PGA1_c03110 0.71 +1.4 300,743 - PGA1_c03110 0.77 -2.0 300,859 + -0.8 300,864 + -1.1 300,906 + -1.0 300,906 + -2.1 300,976 + -1.9 300,976 + -2.3 300,984 - PGA1_c03120 0.11 -2.2 301,036 + PGA1_c03120 0.17 -1.5 301,110 - PGA1_c03120 0.26 -2.2 301,172 - PGA1_c03120 0.33 -2.7 301,274 + PGA1_c03120 0.45 -2.5 301,282 + PGA1_c03120 0.46 -2.9 301,282 - PGA1_c03120 0.46 -2.1 301,302 - PGA1_c03120 0.48 -1.9
Or see this region's nucleotide sequence