Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c24390 and PGA1_c24400 overlap by 4 nucleotides PGA1_c24400 and PGA1_c24410 are separated by 96 nucleotides PGA1_c24410 and PGA1_c24420 are separated by 144 nucleotides PGA1_c24420 and PGA1_c24430 are separated by 44 nucleotides PGA1_c24430 and PGA1_c24440 are separated by 149 nucleotides
PGA1_c24390: PGA1_c24390 - Uncharacterized protein conserved in bacteria, at 2,546,275 to 2,546,664
_c24390
PGA1_c24400: PGA1_c24400 - hypothetical protein, at 2,546,661 to 2,546,927
_c24400
PGA1_c24410: PGA1_c24410 - hypothetical protein, at 2,547,024 to 2,547,305
_c24410
PGA1_c24420: PGA1_c24420 - Uncharacterized conserved protein, at 2,547,450 to 2,547,788
_c24420
PGA1_c24430: PGA1_c24430 - peptidase M48-like protein, at 2,547,833 to 2,548,558
_c24430
PGA1_c24440: PGA1_c24440 - hypothetical protein, at 2,548,708 to 2,548,956
_c24440
Position (kb)
2547
2548 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2546.484 kb on + strand, within PGA1_c24390 at 2546.582 kb on + strand, within PGA1_c24390 at 2546.662 kb on - strand at 2546.679 kb on - strand at 2546.684 kb on - strand at 2546.700 kb on + strand, within PGA1_c24400 at 2546.708 kb on - strand, within PGA1_c24400 at 2546.764 kb on + strand, within PGA1_c24400 at 2546.774 kb on + strand, within PGA1_c24400 at 2546.816 kb on - strand, within PGA1_c24400 at 2546.826 kb on + strand, within PGA1_c24400 at 2546.855 kb on + strand, within PGA1_c24400 at 2546.863 kb on - strand, within PGA1_c24400 at 2546.863 kb on - strand, within PGA1_c24400 at 2546.863 kb on - strand, within PGA1_c24400 at 2546.863 kb on - strand, within PGA1_c24400 at 2546.863 kb on - strand, within PGA1_c24400 at 2546.871 kb on - strand, within PGA1_c24400 at 2546.876 kb on - strand, within PGA1_c24400 at 2546.876 kb on - strand, within PGA1_c24400 at 2546.921 kb on - strand at 2546.946 kb on - strand at 2546.959 kb on + strand at 2547.018 kb on - strand at 2547.018 kb on - strand at 2547.018 kb on - strand at 2547.048 kb on + strand at 2547.056 kb on - strand, within PGA1_c24410 at 2547.056 kb on - strand, within PGA1_c24410 at 2547.091 kb on + strand, within PGA1_c24410 at 2547.215 kb on + strand, within PGA1_c24410 at 2547.215 kb on - strand, within PGA1_c24410 at 2547.249 kb on - strand, within PGA1_c24410 at 2547.280 kb on - strand at 2547.324 kb on + strand at 2547.351 kb on + strand at 2547.359 kb on - strand at 2547.457 kb on + strand at 2547.465 kb on - strand at 2547.467 kb on + strand at 2547.514 kb on + strand, within PGA1_c24420 at 2547.579 kb on - strand, within PGA1_c24420 at 2547.629 kb on + strand, within PGA1_c24420 at 2547.640 kb on + strand, within PGA1_c24420 at 2547.658 kb on + strand, within PGA1_c24420 at 2547.658 kb on + strand, within PGA1_c24420 at 2547.658 kb on + strand, within PGA1_c24420 at 2547.666 kb on - strand, within PGA1_c24420 at 2547.726 kb on - strand, within PGA1_c24420 at 2547.743 kb on - strand, within PGA1_c24420 at 2547.790 kb on + strand at 2547.808 kb on - strand at 2547.811 kb on + strand at 2547.817 kb on + strand at 2547.871 kb on + strand at 2547.887 kb on - strand at 2547.949 kb on + strand, within PGA1_c24430 at 2547.957 kb on - strand, within PGA1_c24430 at 2548.070 kb on + strand, within PGA1_c24430 at 2548.078 kb on - strand, within PGA1_c24430 at 2548.121 kb on + strand, within PGA1_c24430 at 2548.167 kb on + strand, within PGA1_c24430 at 2548.175 kb on - strand, within PGA1_c24430 at 2548.175 kb on - strand, within PGA1_c24430 at 2548.175 kb on - strand, within PGA1_c24430 at 2548.175 kb on - strand, within PGA1_c24430 at 2548.266 kb on - strand, within PGA1_c24430 at 2548.288 kb on - strand, within PGA1_c24430 at 2548.290 kb on + strand, within PGA1_c24430 at 2548.291 kb on - strand, within PGA1_c24430 at 2548.298 kb on - strand, within PGA1_c24430 at 2548.340 kb on + strand, within PGA1_c24430 at 2548.348 kb on - strand, within PGA1_c24430 at 2548.372 kb on - strand, within PGA1_c24430 at 2548.413 kb on + strand, within PGA1_c24430 at 2548.429 kb on - strand, within PGA1_c24430 at 2548.436 kb on - strand, within PGA1_c24430 at 2548.439 kb on + strand, within PGA1_c24430 at 2548.447 kb on - strand, within PGA1_c24430 at 2548.447 kb on - strand, within PGA1_c24430 at 2548.503 kb on - strand at 2548.533 kb on - strand at 2548.535 kb on - strand at 2548.556 kb on + strand at 2548.612 kb on - strand at 2548.626 kb on + strand at 2548.634 kb on - strand at 2548.702 kb on + strand at 2548.707 kb on + strand at 2548.759 kb on + strand, within PGA1_c24440
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 2,546,484 + PGA1_c24390 0.54 +1.0 2,546,582 + PGA1_c24390 0.79 -1.7 2,546,662 - +2.1 2,546,679 - +0.7 2,546,684 - +0.2 2,546,700 + PGA1_c24400 0.15 -1.8 2,546,708 - PGA1_c24400 0.18 +1.7 2,546,764 + PGA1_c24400 0.39 +2.6 2,546,774 + PGA1_c24400 0.42 -1.2 2,546,816 - PGA1_c24400 0.58 -2.0 2,546,826 + PGA1_c24400 0.62 -1.5 2,546,855 + PGA1_c24400 0.73 -2.2 2,546,863 - PGA1_c24400 0.76 +0.9 2,546,863 - PGA1_c24400 0.76 -2.6 2,546,863 - PGA1_c24400 0.76 -0.2 2,546,863 - PGA1_c24400 0.76 +2.1 2,546,863 - PGA1_c24400 0.76 +1.1 2,546,871 - PGA1_c24400 0.79 +1.0 2,546,876 - PGA1_c24400 0.81 +0.6 2,546,876 - PGA1_c24400 0.81 -2.6 2,546,921 - -1.9 2,546,946 - -1.4 2,546,959 + +0.6 2,547,018 - -1.5 2,547,018 - +3.2 2,547,018 - -2.0 2,547,048 + -1.8 2,547,056 - PGA1_c24410 0.11 +0.7 2,547,056 - PGA1_c24410 0.11 +1.2 2,547,091 + PGA1_c24410 0.24 +1.8 2,547,215 + PGA1_c24410 0.68 +0.1 2,547,215 - PGA1_c24410 0.68 -1.2 2,547,249 - PGA1_c24410 0.80 +0.0 2,547,280 - +0.6 2,547,324 + -1.9 2,547,351 + -2.2 2,547,359 - +0.3 2,547,457 + +1.4 2,547,465 - -0.9 2,547,467 + -1.8 2,547,514 + PGA1_c24420 0.19 +0.2 2,547,579 - PGA1_c24420 0.38 -0.0 2,547,629 + PGA1_c24420 0.53 +2.6 2,547,640 + PGA1_c24420 0.56 +1.2 2,547,658 + PGA1_c24420 0.61 +3.0 2,547,658 + PGA1_c24420 0.61 -0.7 2,547,658 + PGA1_c24420 0.61 +2.3 2,547,666 - PGA1_c24420 0.64 +2.4 2,547,726 - PGA1_c24420 0.81 +1.8 2,547,743 - PGA1_c24420 0.86 -2.4 2,547,790 + -2.9 2,547,808 - +3.1 2,547,811 + -0.3 2,547,817 + -1.0 2,547,871 + -0.6 2,547,887 - +1.1 2,547,949 + PGA1_c24430 0.16 -2.6 2,547,957 - PGA1_c24430 0.17 -0.8 2,548,070 + PGA1_c24430 0.33 -1.9 2,548,078 - PGA1_c24430 0.34 -0.2 2,548,121 + PGA1_c24430 0.40 +1.5 2,548,167 + PGA1_c24430 0.46 -1.6 2,548,175 - PGA1_c24430 0.47 -2.7 2,548,175 - PGA1_c24430 0.47 +0.4 2,548,175 - PGA1_c24430 0.47 -0.8 2,548,175 - PGA1_c24430 0.47 +1.3 2,548,266 - PGA1_c24430 0.60 -1.4 2,548,288 - PGA1_c24430 0.63 -1.1 2,548,290 + PGA1_c24430 0.63 -1.5 2,548,291 - PGA1_c24430 0.63 -0.7 2,548,298 - PGA1_c24430 0.64 -1.7 2,548,340 + PGA1_c24430 0.70 +1.0 2,548,348 - PGA1_c24430 0.71 -1.2 2,548,372 - PGA1_c24430 0.74 -0.4 2,548,413 + PGA1_c24430 0.80 -3.0 2,548,429 - PGA1_c24430 0.82 -1.8 2,548,436 - PGA1_c24430 0.83 +1.8 2,548,439 + PGA1_c24430 0.83 +1.6 2,548,447 - PGA1_c24430 0.85 +2.3 2,548,447 - PGA1_c24430 0.85 +2.6 2,548,503 - -0.5 2,548,533 - +0.4 2,548,535 - -1.1 2,548,556 + +2.1 2,548,612 - -0.9 2,548,626 + -2.2 2,548,634 - -2.2 2,548,702 + +0.1 2,548,707 + -0.2 2,548,759 + PGA1_c24440 0.20 -2.5
Or see this region's nucleotide sequence