Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c24280 and PGA1_c24290 are separated by 107 nucleotides PGA1_c24290 and PGA1_c24300 overlap by 4 nucleotides PGA1_c24300 and PGA1_c24310 are separated by 291 nucleotides PGA1_c24310 and PGA1_c24320 overlap by 23 nucleotides
PGA1_c24280: PGA1_c24280 - uncharacterized GST-like protein YghU, at 2,533,493 to 2,534,374
_c24280
PGA1_c24290: PGA1_c24290 - hypothetical protein, at 2,534,482 to 2,535,279
_c24290
PGA1_c24300: PGA1_c24300 - Uracil-DNA glycosylase, at 2,535,276 to 2,535,869
_c24300
PGA1_c24310: PGA1_c24310 - putative capsular polysaccharide transport system, ATP-binding protein, at 2,536,161 to 2,536,820
_c24310
PGA1_c24320: PGA1_c24320 - putative capsule polysaccharide export inner-membrane protein, at 2,536,798 to 2,538,504
_c24320
Position (kb)
2535
2536 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2534.349 kb on - strand at 2534.349 kb on - strand at 2534.421 kb on + strand at 2534.474 kb on - strand at 2534.672 kb on + strand, within PGA1_c24290 at 2534.672 kb on + strand, within PGA1_c24290 at 2534.672 kb on + strand, within PGA1_c24290 at 2534.672 kb on + strand, within PGA1_c24290 at 2534.680 kb on - strand, within PGA1_c24290 at 2534.680 kb on - strand, within PGA1_c24290 at 2534.767 kb on + strand, within PGA1_c24290 at 2534.783 kb on - strand, within PGA1_c24290 at 2534.995 kb on - strand, within PGA1_c24290 at 2535.012 kb on - strand, within PGA1_c24290 at 2535.022 kb on + strand, within PGA1_c24290 at 2535.121 kb on - strand, within PGA1_c24290 at 2535.228 kb on - strand at 2535.285 kb on - strand at 2535.298 kb on - strand at 2535.343 kb on + strand, within PGA1_c24300 at 2535.400 kb on + strand, within PGA1_c24300 at 2535.432 kb on + strand, within PGA1_c24300 at 2535.472 kb on + strand, within PGA1_c24300 at 2535.491 kb on + strand, within PGA1_c24300 at 2535.550 kb on + strand, within PGA1_c24300 at 2535.645 kb on + strand, within PGA1_c24300 at 2535.676 kb on - strand, within PGA1_c24300 at 2535.683 kb on + strand, within PGA1_c24300 at 2535.696 kb on - strand, within PGA1_c24300 at 2535.773 kb on - strand, within PGA1_c24300 at 2535.773 kb on - strand, within PGA1_c24300 at 2535.799 kb on - strand, within PGA1_c24300 at 2535.799 kb on - strand, within PGA1_c24300 at 2535.812 kb on + strand at 2535.817 kb on + strand at 2535.869 kb on + strand at 2535.987 kb on + strand at 2536.040 kb on - strand at 2536.053 kb on - strand at 2536.078 kb on + strand at 2536.078 kb on + strand at 2536.085 kb on - strand at 2536.086 kb on - strand at 2536.100 kb on - strand at 2536.101 kb on - strand at 2536.115 kb on + strand at 2536.122 kb on - strand at 2536.123 kb on - strand at 2536.124 kb on + strand at 2536.124 kb on + strand at 2536.134 kb on - strand at 2536.136 kb on + strand at 2536.136 kb on + strand at 2536.144 kb on - strand at 2536.144 kb on - strand at 2536.144 kb on - strand at 2536.159 kb on + strand at 2536.240 kb on + strand, within PGA1_c24310 at 2536.248 kb on - strand, within PGA1_c24310 at 2536.248 kb on - strand, within PGA1_c24310 at 2536.294 kb on - strand, within PGA1_c24310 at 2536.296 kb on + strand, within PGA1_c24310 at 2536.315 kb on - strand, within PGA1_c24310 at 2536.365 kb on - strand, within PGA1_c24310 at 2536.415 kb on + strand, within PGA1_c24310 at 2536.512 kb on - strand, within PGA1_c24310 at 2536.512 kb on - strand, within PGA1_c24310 at 2536.512 kb on - strand, within PGA1_c24310 at 2536.550 kb on + strand, within PGA1_c24310 at 2536.558 kb on - strand, within PGA1_c24310 at 2536.558 kb on - strand, within PGA1_c24310 at 2536.610 kb on + strand, within PGA1_c24310 at 2536.675 kb on - strand, within PGA1_c24310 at 2536.703 kb on + strand, within PGA1_c24310 at 2536.788 kb on + strand at 2536.793 kb on + strand at 2536.793 kb on + strand at 2536.793 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 2,534,349 - +2.0 2,534,349 - -2.2 2,534,421 + +0.6 2,534,474 - +1.5 2,534,672 + PGA1_c24290 0.24 -1.5 2,534,672 + PGA1_c24290 0.24 -1.2 2,534,672 + PGA1_c24290 0.24 +2.3 2,534,672 + PGA1_c24290 0.24 +1.1 2,534,680 - PGA1_c24290 0.25 +1.9 2,534,680 - PGA1_c24290 0.25 -2.0 2,534,767 + PGA1_c24290 0.36 +0.9 2,534,783 - PGA1_c24290 0.38 +3.1 2,534,995 - PGA1_c24290 0.64 -0.4 2,535,012 - PGA1_c24290 0.66 -0.3 2,535,022 + PGA1_c24290 0.68 -1.3 2,535,121 - PGA1_c24290 0.80 -0.8 2,535,228 - +0.7 2,535,285 - -1.9 2,535,298 - +1.9 2,535,343 + PGA1_c24300 0.11 -3.3 2,535,400 + PGA1_c24300 0.21 -2.6 2,535,432 + PGA1_c24300 0.26 +2.7 2,535,472 + PGA1_c24300 0.33 -0.4 2,535,491 + PGA1_c24300 0.36 -1.9 2,535,550 + PGA1_c24300 0.46 -1.4 2,535,645 + PGA1_c24300 0.62 -3.2 2,535,676 - PGA1_c24300 0.67 -2.2 2,535,683 + PGA1_c24300 0.69 -2.8 2,535,696 - PGA1_c24300 0.71 +3.6 2,535,773 - PGA1_c24300 0.84 -2.3 2,535,773 - PGA1_c24300 0.84 -0.2 2,535,799 - PGA1_c24300 0.88 +2.2 2,535,799 - PGA1_c24300 0.88 -2.1 2,535,812 + -2.5 2,535,817 + -0.1 2,535,869 + -2.6 2,535,987 + +1.6 2,536,040 - -1.9 2,536,053 - +1.6 2,536,078 + +2.4 2,536,078 + +1.7 2,536,085 - -2.6 2,536,086 - +1.6 2,536,100 - +0.8 2,536,101 - -1.3 2,536,115 + +3.2 2,536,122 - -2.7 2,536,123 - -0.9 2,536,124 + -2.4 2,536,124 + +2.7 2,536,134 - -2.5 2,536,136 + +0.4 2,536,136 + -0.6 2,536,144 - -1.0 2,536,144 - +1.2 2,536,144 - +3.2 2,536,159 + -0.9 2,536,240 + PGA1_c24310 0.12 -2.1 2,536,248 - PGA1_c24310 0.13 +2.4 2,536,248 - PGA1_c24310 0.13 +2.5 2,536,294 - PGA1_c24310 0.20 +1.7 2,536,296 + PGA1_c24310 0.20 +2.0 2,536,315 - PGA1_c24310 0.23 -2.1 2,536,365 - PGA1_c24310 0.31 -2.6 2,536,415 + PGA1_c24310 0.38 -0.3 2,536,512 - PGA1_c24310 0.53 -1.0 2,536,512 - PGA1_c24310 0.53 +2.4 2,536,512 - PGA1_c24310 0.53 +2.2 2,536,550 + PGA1_c24310 0.59 +2.6 2,536,558 - PGA1_c24310 0.60 +2.1 2,536,558 - PGA1_c24310 0.60 +2.0 2,536,610 + PGA1_c24310 0.68 +1.9 2,536,675 - PGA1_c24310 0.78 -2.8 2,536,703 + PGA1_c24310 0.82 -1.8 2,536,788 + -2.6 2,536,793 + -2.8 2,536,793 + -1.5 2,536,793 + +2.8
Or see this region's nucleotide sequence