Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02480 and PGA1_c02490 are separated by 16 nucleotides PGA1_c02490 and PGA1_c02500 are separated by 7 nucleotides PGA1_c02500 and PGA1_c02510 are separated by 163 nucleotides
PGA1_c02480: PGA1_c02480 - Uncharacterized conserved protein, at 230,949 to 232,286
_c02480
PGA1_c02490: PGA1_c02490 - sporulation protein R-like protein, at 232,303 to 233,781
_c02490
PGA1_c02500: PGA1_c02500 - transcriptional regulator, GntR family, at 233,789 to 234,454
_c02500
PGA1_c02510: PGA1_c02510 - S-(hydroxymethyl)glutathione dehydrogenase FrmA, at 234,618 to 235,733
_c02510
Position (kb) 232
233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 231.346 kb on + strand, within PGA1_c02480 at 231.346 kb on + strand, within PGA1_c02480 at 231.346 kb on + strand, within PGA1_c02480 at 231.346 kb on + strand, within PGA1_c02480 at 231.346 kb on + strand, within PGA1_c02480 at 231.354 kb on - strand, within PGA1_c02480 at 231.378 kb on + strand, within PGA1_c02480 at 231.378 kb on + strand, within PGA1_c02480 at 231.414 kb on + strand, within PGA1_c02480 at 231.422 kb on - strand, within PGA1_c02480 at 231.422 kb on - strand, within PGA1_c02480 at 231.443 kb on + strand, within PGA1_c02480 at 231.484 kb on + strand, within PGA1_c02480 at 231.497 kb on + strand, within PGA1_c02480 at 231.502 kb on + strand, within PGA1_c02480 at 231.502 kb on + strand, within PGA1_c02480 at 231.543 kb on + strand, within PGA1_c02480 at 231.584 kb on + strand, within PGA1_c02480 at 231.586 kb on - strand, within PGA1_c02480 at 231.608 kb on + strand, within PGA1_c02480 at 231.615 kb on - strand, within PGA1_c02480 at 231.616 kb on - strand, within PGA1_c02480 at 231.618 kb on - strand, within PGA1_c02480 at 231.623 kb on - strand, within PGA1_c02480 at 231.660 kb on + strand, within PGA1_c02480 at 231.722 kb on - strand, within PGA1_c02480 at 231.742 kb on + strand, within PGA1_c02480 at 231.744 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.811 kb on + strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.819 kb on - strand, within PGA1_c02480 at 231.860 kb on + strand, within PGA1_c02480 at 231.878 kb on - strand, within PGA1_c02480 at 231.912 kb on + strand, within PGA1_c02480 at 231.919 kb on - strand, within PGA1_c02480 at 231.946 kb on + strand, within PGA1_c02480 at 231.977 kb on + strand, within PGA1_c02480 at 231.977 kb on + strand, within PGA1_c02480 at 231.977 kb on + strand, within PGA1_c02480 at 231.985 kb on - strand, within PGA1_c02480 at 231.985 kb on - strand, within PGA1_c02480 at 232.031 kb on + strand, within PGA1_c02480 at 232.031 kb on + strand, within PGA1_c02480 at 232.041 kb on - strand, within PGA1_c02480 at 232.073 kb on + strand, within PGA1_c02480 at 232.073 kb on + strand, within PGA1_c02480 at 232.073 kb on + strand, within PGA1_c02480 at 232.126 kb on + strand, within PGA1_c02480 at 232.134 kb on - strand, within PGA1_c02480 at 232.134 kb on - strand, within PGA1_c02480 at 232.134 kb on - strand, within PGA1_c02480 at 232.134 kb on - strand, within PGA1_c02480 at 232.134 kb on - strand, within PGA1_c02480 at 232.166 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.256 kb on + strand at 232.256 kb on + strand at 232.282 kb on + strand at 232.318 kb on + strand at 232.326 kb on - strand at 232.343 kb on - strand at 232.392 kb on + strand at 232.400 kb on - strand at 232.405 kb on - strand at 232.416 kb on + strand at 232.416 kb on - strand at 232.441 kb on + strand at 232.518 kb on + strand, within PGA1_c02490 at 232.536 kb on - strand, within PGA1_c02490 at 232.537 kb on + strand, within PGA1_c02490 at 232.537 kb on + strand, within PGA1_c02490 at 232.545 kb on - strand, within PGA1_c02490 at 232.614 kb on - strand, within PGA1_c02490 at 232.680 kb on + strand, within PGA1_c02490 at 232.685 kb on + strand, within PGA1_c02490 at 232.693 kb on - strand, within PGA1_c02490 at 232.693 kb on - strand, within PGA1_c02490 at 232.693 kb on - strand, within PGA1_c02490 at 232.704 kb on - strand, within PGA1_c02490 at 232.753 kb on - strand, within PGA1_c02490 at 232.754 kb on + strand, within PGA1_c02490 at 232.814 kb on - strand, within PGA1_c02490 at 232.850 kb on + strand, within PGA1_c02490 at 232.944 kb on - strand, within PGA1_c02490 at 232.946 kb on + strand, within PGA1_c02490 at 232.946 kb on + strand, within PGA1_c02490 at 232.946 kb on + strand, within PGA1_c02490 at 232.954 kb on - strand, within PGA1_c02490 at 232.954 kb on - strand, within PGA1_c02490 at 232.956 kb on + strand, within PGA1_c02490 at 232.956 kb on + strand, within PGA1_c02490 at 232.956 kb on + strand, within PGA1_c02490 at 232.956 kb on + strand, within PGA1_c02490 at 232.964 kb on - strand, within PGA1_c02490 at 232.964 kb on - strand, within PGA1_c02490 at 232.964 kb on - strand, within PGA1_c02490 at 232.964 kb on - strand, within PGA1_c02490 at 232.964 kb on - strand, within PGA1_c02490 at 232.964 kb on - strand, within PGA1_c02490 at 232.964 kb on - strand, within PGA1_c02490 at 232.964 kb on - strand, within PGA1_c02490 at 232.964 kb on - strand, within PGA1_c02490 at 232.968 kb on + strand, within PGA1_c02490 at 232.976 kb on - strand, within PGA1_c02490 at 232.976 kb on - strand, within PGA1_c02490 at 232.976 kb on - strand, within PGA1_c02490 at 232.976 kb on - strand, within PGA1_c02490 at 233.046 kb on + strand, within PGA1_c02490 at 233.047 kb on - strand, within PGA1_c02490 at 233.054 kb on - strand, within PGA1_c02490 at 233.054 kb on - strand, within PGA1_c02490 at 233.054 kb on - strand, within PGA1_c02490 at 233.070 kb on - strand, within PGA1_c02490 at 233.078 kb on - strand, within PGA1_c02490 at 233.093 kb on - strand, within PGA1_c02490 at 233.139 kb on - strand, within PGA1_c02490 at 233.186 kb on - strand, within PGA1_c02490 at 233.192 kb on + strand, within PGA1_c02490 at 233.196 kb on + strand, within PGA1_c02490 at 233.228 kb on + strand, within PGA1_c02490 at 233.235 kb on - strand, within PGA1_c02490 at 233.235 kb on - strand, within PGA1_c02490 at 233.242 kb on - strand, within PGA1_c02490 at 233.244 kb on + strand, within PGA1_c02490 at 233.325 kb on + strand, within PGA1_c02490 at 233.353 kb on + strand, within PGA1_c02490 at 233.426 kb on + strand, within PGA1_c02490 at 233.459 kb on - strand, within PGA1_c02490 at 233.495 kb on - strand, within PGA1_c02490 at 233.572 kb on + strand, within PGA1_c02490 at 233.603 kb on - strand, within PGA1_c02490 at 233.634 kb on + strand at 233.634 kb on + strand at 233.634 kb on + strand at 233.634 kb on + strand at 233.642 kb on - strand at 233.642 kb on - strand at 233.669 kb on - strand at 233.681 kb on + strand at 233.732 kb on + strand at 233.732 kb on + strand at 233.732 kb on + strand at 233.732 kb on + strand at 233.732 kb on + strand at 233.732 kb on + strand at 233.732 kb on + strand at 233.740 kb on - strand at 233.740 kb on - strand at 233.740 kb on - strand at 233.740 kb on - strand at 233.761 kb on - strand at 233.795 kb on + strand at 233.812 kb on - strand at 233.911 kb on + strand, within PGA1_c02500 at 234.014 kb on - strand, within PGA1_c02500 at 234.019 kb on + strand, within PGA1_c02500 at 234.034 kb on - strand, within PGA1_c02500 at 234.133 kb on + strand, within PGA1_c02500 at 234.146 kb on - strand, within PGA1_c02500 at 234.237 kb on + strand, within PGA1_c02500 at 234.245 kb on - strand, within PGA1_c02500 at 234.245 kb on - strand, within PGA1_c02500 at 234.255 kb on - strand, within PGA1_c02500 at 234.266 kb on + strand, within PGA1_c02500 at 234.271 kb on + strand, within PGA1_c02500 at 234.304 kb on + strand, within PGA1_c02500 at 234.423 kb on + strand at 234.484 kb on - strand at 234.590 kb on + strand at 234.600 kb on - strand at 234.662 kb on + strand at 234.695 kb on + strand at 234.695 kb on + strand at 234.703 kb on - strand at 234.703 kb on - strand at 234.703 kb on - strand at 234.734 kb on + strand, within PGA1_c02510 at 234.742 kb on - strand, within PGA1_c02510 at 234.742 kb on - strand, within PGA1_c02510 at 234.742 kb on - strand, within PGA1_c02510 at 234.759 kb on - strand, within PGA1_c02510 at 234.764 kb on - strand, within PGA1_c02510
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 231,346 + PGA1_c02480 0.30 +1.4 231,346 + PGA1_c02480 0.30 -0.4 231,346 + PGA1_c02480 0.30 -1.1 231,346 + PGA1_c02480 0.30 +2.3 231,346 + PGA1_c02480 0.30 -1.8 231,354 - PGA1_c02480 0.30 +2.2 231,378 + PGA1_c02480 0.32 -1.5 231,378 + PGA1_c02480 0.32 -1.9 231,414 + PGA1_c02480 0.35 -1.9 231,422 - PGA1_c02480 0.35 -2.3 231,422 - PGA1_c02480 0.35 -0.9 231,443 + PGA1_c02480 0.37 +2.8 231,484 + PGA1_c02480 0.40 +2.9 231,497 + PGA1_c02480 0.41 -1.9 231,502 + PGA1_c02480 0.41 +2.0 231,502 + PGA1_c02480 0.41 +0.3 231,543 + PGA1_c02480 0.44 -1.1 231,584 + PGA1_c02480 0.47 +2.2 231,586 - PGA1_c02480 0.48 -2.5 231,608 + PGA1_c02480 0.49 +2.4 231,615 - PGA1_c02480 0.50 +2.4 231,616 - PGA1_c02480 0.50 +2.1 231,618 - PGA1_c02480 0.50 -2.0 231,623 - PGA1_c02480 0.50 +0.3 231,660 + PGA1_c02480 0.53 +1.7 231,722 - PGA1_c02480 0.58 +1.1 231,742 + PGA1_c02480 0.59 -1.5 231,744 + PGA1_c02480 0.59 +0.2 231,811 + PGA1_c02480 0.64 +0.8 231,811 + PGA1_c02480 0.64 -1.2 231,811 + PGA1_c02480 0.64 +2.4 231,811 + PGA1_c02480 0.64 +2.7 231,811 + PGA1_c02480 0.64 +2.1 231,811 + PGA1_c02480 0.64 +0.9 231,811 + PGA1_c02480 0.64 +1.8 231,811 + PGA1_c02480 0.64 -1.1 231,811 + PGA1_c02480 0.64 -1.8 231,811 + PGA1_c02480 0.64 +1.4 231,819 - PGA1_c02480 0.65 +0.9 231,819 - PGA1_c02480 0.65 +1.5 231,819 - PGA1_c02480 0.65 +3.6 231,819 - PGA1_c02480 0.65 -2.8 231,819 - PGA1_c02480 0.65 +0.5 231,819 - PGA1_c02480 0.65 -0.2 231,819 - PGA1_c02480 0.65 -2.7 231,819 - PGA1_c02480 0.65 -1.1 231,819 - PGA1_c02480 0.65 -1.8 231,819 - PGA1_c02480 0.65 -1.2 231,819 - PGA1_c02480 0.65 -1.2 231,819 - PGA1_c02480 0.65 +2.2 231,819 - PGA1_c02480 0.65 -0.6 231,819 - PGA1_c02480 0.65 +4.7 231,819 - PGA1_c02480 0.65 +1.0 231,819 - PGA1_c02480 0.65 +2.3 231,819 - PGA1_c02480 0.65 +0.7 231,819 - PGA1_c02480 0.65 +2.4 231,860 + PGA1_c02480 0.68 +1.8 231,878 - PGA1_c02480 0.69 +1.1 231,912 + PGA1_c02480 0.72 -0.7 231,919 - PGA1_c02480 0.72 -1.7 231,946 + PGA1_c02480 0.75 -2.3 231,977 + PGA1_c02480 0.77 -0.2 231,977 + PGA1_c02480 0.77 +1.8 231,977 + PGA1_c02480 0.77 +2.0 231,985 - PGA1_c02480 0.77 -2.0 231,985 - PGA1_c02480 0.77 +3.2 232,031 + PGA1_c02480 0.81 +2.1 232,031 + PGA1_c02480 0.81 +0.7 232,041 - PGA1_c02480 0.82 -1.2 232,073 + PGA1_c02480 0.84 -1.5 232,073 + PGA1_c02480 0.84 -3.3 232,073 + PGA1_c02480 0.84 -2.3 232,126 + PGA1_c02480 0.88 -0.6 232,134 - PGA1_c02480 0.89 -2.2 232,134 - PGA1_c02480 0.89 +3.0 232,134 - PGA1_c02480 0.89 -2.1 232,134 - PGA1_c02480 0.89 +2.2 232,134 - PGA1_c02480 0.89 +1.2 232,166 + +2.5 232,172 + -0.5 232,172 + -0.8 232,256 + -2.0 232,256 + +1.2 232,282 + +2.5 232,318 + -1.2 232,326 - -0.5 232,343 - -0.4 232,392 + -1.6 232,400 - -1.9 232,405 - +2.3 232,416 + +0.2 232,416 - -2.4 232,441 + +0.8 232,518 + PGA1_c02490 0.15 -1.6 232,536 - PGA1_c02490 0.16 -0.8 232,537 + PGA1_c02490 0.16 +2.1 232,537 + PGA1_c02490 0.16 -0.7 232,545 - PGA1_c02490 0.16 +2.7 232,614 - PGA1_c02490 0.21 -1.0 232,680 + PGA1_c02490 0.25 -2.1 232,685 + PGA1_c02490 0.26 +1.2 232,693 - PGA1_c02490 0.26 +1.8 232,693 - PGA1_c02490 0.26 +0.3 232,693 - PGA1_c02490 0.26 +0.5 232,704 - PGA1_c02490 0.27 +2.8 232,753 - PGA1_c02490 0.30 +2.3 232,754 + PGA1_c02490 0.30 -1.7 232,814 - PGA1_c02490 0.35 +3.5 232,850 + PGA1_c02490 0.37 +1.0 232,944 - PGA1_c02490 0.43 -1.9 232,946 + PGA1_c02490 0.43 +1.5 232,946 + PGA1_c02490 0.43 +3.3 232,946 + PGA1_c02490 0.43 -0.6 232,954 - PGA1_c02490 0.44 +0.1 232,954 - PGA1_c02490 0.44 +2.2 232,956 + PGA1_c02490 0.44 +2.4 232,956 + PGA1_c02490 0.44 -0.7 232,956 + PGA1_c02490 0.44 -2.2 232,956 + PGA1_c02490 0.44 -2.6 232,964 - PGA1_c02490 0.45 +1.5 232,964 - PGA1_c02490 0.45 -1.1 232,964 - PGA1_c02490 0.45 -1.8 232,964 - PGA1_c02490 0.45 +4.5 232,964 - PGA1_c02490 0.45 -2.8 232,964 - PGA1_c02490 0.45 -1.0 232,964 - PGA1_c02490 0.45 +2.0 232,964 - PGA1_c02490 0.45 +0.0 232,964 - PGA1_c02490 0.45 -2.7 232,968 + PGA1_c02490 0.45 -2.0 232,976 - PGA1_c02490 0.46 -1.2 232,976 - PGA1_c02490 0.46 -1.8 232,976 - PGA1_c02490 0.46 -1.8 232,976 - PGA1_c02490 0.46 +3.0 233,046 + PGA1_c02490 0.50 -3.2 233,047 - PGA1_c02490 0.50 -1.0 233,054 - PGA1_c02490 0.51 -1.3 233,054 - PGA1_c02490 0.51 +1.9 233,054 - PGA1_c02490 0.51 -1.5 233,070 - PGA1_c02490 0.52 +1.1 233,078 - PGA1_c02490 0.52 -1.6 233,093 - PGA1_c02490 0.53 -0.7 233,139 - PGA1_c02490 0.57 +2.4 233,186 - PGA1_c02490 0.60 -1.4 233,192 + PGA1_c02490 0.60 -0.1 233,196 + PGA1_c02490 0.60 -1.3 233,228 + PGA1_c02490 0.63 -1.8 233,235 - PGA1_c02490 0.63 +2.0 233,235 - PGA1_c02490 0.63 -2.3 233,242 - PGA1_c02490 0.63 +0.8 233,244 + PGA1_c02490 0.64 +2.7 233,325 + PGA1_c02490 0.69 +0.1 233,353 + PGA1_c02490 0.71 +1.1 233,426 + PGA1_c02490 0.76 +2.8 233,459 - PGA1_c02490 0.78 -0.7 233,495 - PGA1_c02490 0.81 -0.6 233,572 + PGA1_c02490 0.86 +1.4 233,603 - PGA1_c02490 0.88 +0.0 233,634 + +3.2 233,634 + +0.8 233,634 + +5.5 233,634 + +2.6 233,642 - -2.2 233,642 - +2.0 233,669 - +3.3 233,681 + -0.9 233,732 + +1.5 233,732 + +4.0 233,732 + +1.6 233,732 + +1.9 233,732 + -2.3 233,732 + -2.5 233,732 + -2.6 233,740 - +2.0 233,740 - +0.1 233,740 - -2.5 233,740 - -1.7 233,761 - -0.8 233,795 + +0.4 233,812 - -1.2 233,911 + PGA1_c02500 0.18 +1.9 234,014 - PGA1_c02500 0.34 -2.6 234,019 + PGA1_c02500 0.35 -2.3 234,034 - PGA1_c02500 0.37 -1.8 234,133 + PGA1_c02500 0.52 -0.6 234,146 - PGA1_c02500 0.54 -1.0 234,237 + PGA1_c02500 0.67 -1.0 234,245 - PGA1_c02500 0.68 -1.3 234,245 - PGA1_c02500 0.68 +1.5 234,255 - PGA1_c02500 0.70 -1.9 234,266 + PGA1_c02500 0.72 +2.7 234,271 + PGA1_c02500 0.72 +3.0 234,304 + PGA1_c02500 0.77 +2.5 234,423 + -0.9 234,484 - -1.1 234,590 + -1.6 234,600 - +2.3 234,662 + +2.1 234,695 + -1.1 234,695 + -0.8 234,703 - +1.7 234,703 - -2.5 234,703 - +0.2 234,734 + PGA1_c02510 0.10 +0.8 234,742 - PGA1_c02510 0.11 +2.0 234,742 - PGA1_c02510 0.11 -2.4 234,742 - PGA1_c02510 0.11 -0.6 234,759 - PGA1_c02510 0.13 -1.6 234,764 - PGA1_c02510 0.13 +0.3
Or see this region's nucleotide sequence