Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c22910 and PGA1_c22920 are separated by 2 nucleotides PGA1_c22920 and PGA1_c22930 overlap by 8 nucleotides PGA1_c22930 and PGA1_c22940 are separated by 20 nucleotides
PGA1_c22910: PGA1_c22910 - putative type IV secretion system protein VirB10, at 2,373,089 to 2,374,402
_c22910
PGA1_c22920: PGA1_c22920 - putative type IV secretion system protein VirB11, at 2,374,405 to 2,375,400
_c22920
PGA1_c22930: PGA1_c22930 - hypothetical protein, at 2,375,393 to 2,375,569
_c22930
PGA1_c22940: PGA1_c22940 - putative type IV secretion system protein VirB6, at 2,375,590 to 2,376,588
_c22940
Position (kb)
2374
2375
2376 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2373.478 kb on - strand, within PGA1_c22910 at 2373.609 kb on - strand, within PGA1_c22910 at 2373.626 kb on - strand, within PGA1_c22910 at 2373.698 kb on + strand, within PGA1_c22910 at 2373.821 kb on - strand, within PGA1_c22910 at 2373.821 kb on - strand, within PGA1_c22910 at 2373.896 kb on + strand, within PGA1_c22910 at 2373.937 kb on - strand, within PGA1_c22910 at 2373.937 kb on - strand, within PGA1_c22910 at 2373.937 kb on - strand, within PGA1_c22910 at 2373.999 kb on + strand, within PGA1_c22910 at 2374.004 kb on + strand, within PGA1_c22910 at 2374.028 kb on + strand, within PGA1_c22910 at 2374.124 kb on + strand, within PGA1_c22910 at 2374.190 kb on - strand, within PGA1_c22910 at 2374.193 kb on + strand, within PGA1_c22910 at 2374.198 kb on - strand, within PGA1_c22910 at 2374.221 kb on - strand, within PGA1_c22910 at 2374.229 kb on + strand, within PGA1_c22910 at 2374.237 kb on - strand, within PGA1_c22910 at 2374.237 kb on - strand, within PGA1_c22910 at 2374.237 kb on - strand, within PGA1_c22910 at 2374.237 kb on - strand, within PGA1_c22910 at 2374.262 kb on - strand, within PGA1_c22910 at 2374.285 kb on + strand at 2374.285 kb on - strand at 2374.286 kb on + strand at 2374.294 kb on - strand at 2374.294 kb on - strand at 2374.321 kb on + strand at 2374.435 kb on - strand at 2374.556 kb on - strand, within PGA1_c22920 at 2374.619 kb on - strand, within PGA1_c22920 at 2374.620 kb on - strand, within PGA1_c22920 at 2374.625 kb on - strand, within PGA1_c22920 at 2374.765 kb on - strand, within PGA1_c22920 at 2374.766 kb on - strand, within PGA1_c22920 at 2374.891 kb on + strand, within PGA1_c22920 at 2374.930 kb on - strand, within PGA1_c22920 at 2375.018 kb on - strand, within PGA1_c22920 at 2375.018 kb on - strand, within PGA1_c22920 at 2375.057 kb on + strand, within PGA1_c22920 at 2375.065 kb on - strand, within PGA1_c22920 at 2375.075 kb on + strand, within PGA1_c22920 at 2375.075 kb on + strand, within PGA1_c22920 at 2375.075 kb on + strand, within PGA1_c22920 at 2375.075 kb on + strand, within PGA1_c22920 at 2375.083 kb on - strand, within PGA1_c22920 at 2375.083 kb on - strand, within PGA1_c22920 at 2375.083 kb on - strand, within PGA1_c22920 at 2375.083 kb on - strand, within PGA1_c22920 at 2375.096 kb on - strand at 2375.142 kb on - strand, within PGA1_c22920 at 2375.151 kb on - strand, within PGA1_c22920 at 2375.264 kb on + strand, within PGA1_c22920 at 2375.307 kb on + strand at 2375.307 kb on + strand at 2375.315 kb on - strand at 2375.315 kb on - strand at 2375.383 kb on - strand at 2375.406 kb on + strand at 2375.406 kb on + strand at 2375.414 kb on - strand, within PGA1_c22930 at 2375.416 kb on + strand, within PGA1_c22930 at 2375.416 kb on + strand, within PGA1_c22930 at 2375.416 kb on + strand, within PGA1_c22930 at 2375.416 kb on + strand, within PGA1_c22930 at 2375.416 kb on + strand, within PGA1_c22930 at 2375.416 kb on + strand, within PGA1_c22930 at 2375.424 kb on - strand, within PGA1_c22930 at 2375.424 kb on - strand, within PGA1_c22930 at 2375.424 kb on - strand, within PGA1_c22930 at 2375.424 kb on - strand, within PGA1_c22930 at 2375.424 kb on - strand, within PGA1_c22930 at 2375.424 kb on - strand, within PGA1_c22930 at 2375.454 kb on - strand, within PGA1_c22930 at 2375.459 kb on - strand, within PGA1_c22930 at 2375.486 kb on + strand, within PGA1_c22930 at 2375.491 kb on + strand, within PGA1_c22930 at 2375.494 kb on - strand, within PGA1_c22930 at 2375.652 kb on - strand at 2375.652 kb on - strand at 2375.652 kb on - strand at 2375.652 kb on - strand at 2375.661 kb on - strand at 2375.684 kb on - strand at 2375.770 kb on + strand, within PGA1_c22940 at 2375.832 kb on - strand, within PGA1_c22940 at 2375.837 kb on - strand, within PGA1_c22940 at 2375.903 kb on - strand, within PGA1_c22940 at 2375.935 kb on + strand, within PGA1_c22940 at 2375.939 kb on + strand, within PGA1_c22940 at 2375.978 kb on - strand, within PGA1_c22940 at 2376.003 kb on + strand, within PGA1_c22940 at 2376.010 kb on + strand, within PGA1_c22940 at 2376.018 kb on - strand, within PGA1_c22940 at 2376.018 kb on - strand, within PGA1_c22940 at 2376.018 kb on - strand, within PGA1_c22940 at 2376.018 kb on - strand, within PGA1_c22940 at 2376.018 kb on - strand, within PGA1_c22940 at 2376.058 kb on + strand, within PGA1_c22940 at 2376.070 kb on + strand, within PGA1_c22940 at 2376.070 kb on + strand, within PGA1_c22940 at 2376.070 kb on + strand, within PGA1_c22940 at 2376.070 kb on + strand, within PGA1_c22940 at 2376.070 kb on + strand, within PGA1_c22940 at 2376.078 kb on - strand, within PGA1_c22940 at 2376.078 kb on - strand, within PGA1_c22940 at 2376.078 kb on - strand, within PGA1_c22940 at 2376.110 kb on + strand, within PGA1_c22940 at 2376.110 kb on + strand, within PGA1_c22940 at 2376.157 kb on + strand, within PGA1_c22940 at 2376.157 kb on + strand, within PGA1_c22940 at 2376.177 kb on + strand, within PGA1_c22940 at 2376.189 kb on + strand, within PGA1_c22940 at 2376.199 kb on - strand, within PGA1_c22940 at 2376.241 kb on + strand, within PGA1_c22940 at 2376.243 kb on - strand, within PGA1_c22940 at 2376.304 kb on + strand, within PGA1_c22940
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 2,373,478 - PGA1_c22910 0.30 +0.5 2,373,609 - PGA1_c22910 0.40 -1.2 2,373,626 - PGA1_c22910 0.41 -2.0 2,373,698 + PGA1_c22910 0.46 -2.4 2,373,821 - PGA1_c22910 0.56 +2.0 2,373,821 - PGA1_c22910 0.56 -2.8 2,373,896 + PGA1_c22910 0.61 -2.8 2,373,937 - PGA1_c22910 0.65 +1.6 2,373,937 - PGA1_c22910 0.65 -2.4 2,373,937 - PGA1_c22910 0.65 +2.4 2,373,999 + PGA1_c22910 0.69 -0.9 2,374,004 + PGA1_c22910 0.70 -3.2 2,374,028 + PGA1_c22910 0.71 +3.7 2,374,124 + PGA1_c22910 0.79 -0.3 2,374,190 - PGA1_c22910 0.84 -1.0 2,374,193 + PGA1_c22910 0.84 -2.6 2,374,198 - PGA1_c22910 0.84 -1.3 2,374,221 - PGA1_c22910 0.86 +0.2 2,374,229 + PGA1_c22910 0.87 -0.6 2,374,237 - PGA1_c22910 0.87 +2.6 2,374,237 - PGA1_c22910 0.87 -1.7 2,374,237 - PGA1_c22910 0.87 -0.8 2,374,237 - PGA1_c22910 0.87 +1.1 2,374,262 - PGA1_c22910 0.89 -0.8 2,374,285 + -1.3 2,374,285 - -2.2 2,374,286 + -1.5 2,374,294 - -0.6 2,374,294 - +1.1 2,374,321 + +1.6 2,374,435 - -0.9 2,374,556 - PGA1_c22920 0.15 -4.0 2,374,619 - PGA1_c22920 0.21 -1.7 2,374,620 - PGA1_c22920 0.22 +1.1 2,374,625 - PGA1_c22920 0.22 -0.4 2,374,765 - PGA1_c22920 0.36 +1.3 2,374,766 - PGA1_c22920 0.36 -1.0 2,374,891 + PGA1_c22920 0.49 +0.1 2,374,930 - PGA1_c22920 0.53 -1.5 2,375,018 - PGA1_c22920 0.62 +1.7 2,375,018 - PGA1_c22920 0.62 +0.8 2,375,057 + PGA1_c22920 0.65 -2.3 2,375,065 - PGA1_c22920 0.66 +0.6 2,375,075 + PGA1_c22920 0.67 -1.3 2,375,075 + PGA1_c22920 0.67 +2.6 2,375,075 + PGA1_c22920 0.67 +0.9 2,375,075 + PGA1_c22920 0.67 +1.6 2,375,083 - PGA1_c22920 0.68 -1.5 2,375,083 - PGA1_c22920 0.68 -0.4 2,375,083 - PGA1_c22920 0.68 +2.5 2,375,083 - PGA1_c22920 0.68 +2.1 2,375,096 - +0.4 2,375,142 - PGA1_c22920 0.74 -1.2 2,375,151 - PGA1_c22920 0.75 -2.0 2,375,264 + PGA1_c22920 0.86 -1.8 2,375,307 + -2.1 2,375,307 + -1.5 2,375,315 - -2.3 2,375,315 - -0.3 2,375,383 - +1.7 2,375,406 + +0.2 2,375,406 + -1.1 2,375,414 - PGA1_c22930 0.12 -1.7 2,375,416 + PGA1_c22930 0.13 +1.0 2,375,416 + PGA1_c22930 0.13 -1.4 2,375,416 + PGA1_c22930 0.13 -2.2 2,375,416 + PGA1_c22930 0.13 +1.4 2,375,416 + PGA1_c22930 0.13 +1.6 2,375,416 + PGA1_c22930 0.13 -2.1 2,375,424 - PGA1_c22930 0.18 -0.6 2,375,424 - PGA1_c22930 0.18 +0.8 2,375,424 - PGA1_c22930 0.18 -0.1 2,375,424 - PGA1_c22930 0.18 -2.1 2,375,424 - PGA1_c22930 0.18 +1.4 2,375,424 - PGA1_c22930 0.18 -0.8 2,375,454 - PGA1_c22930 0.34 +2.0 2,375,459 - PGA1_c22930 0.37 -1.4 2,375,486 + PGA1_c22930 0.53 +2.3 2,375,491 + PGA1_c22930 0.55 -1.2 2,375,494 - PGA1_c22930 0.57 -1.4 2,375,652 - +3.0 2,375,652 - -1.5 2,375,652 - -1.8 2,375,652 - +1.5 2,375,661 - -1.9 2,375,684 - +4.1 2,375,770 + PGA1_c22940 0.18 -0.5 2,375,832 - PGA1_c22940 0.24 +1.7 2,375,837 - PGA1_c22940 0.25 -1.0 2,375,903 - PGA1_c22940 0.31 -0.3 2,375,935 + PGA1_c22940 0.35 +4.5 2,375,939 + PGA1_c22940 0.35 -1.8 2,375,978 - PGA1_c22940 0.39 -1.8 2,376,003 + PGA1_c22940 0.41 -0.5 2,376,010 + PGA1_c22940 0.42 +2.6 2,376,018 - PGA1_c22940 0.43 +2.8 2,376,018 - PGA1_c22940 0.43 +2.2 2,376,018 - PGA1_c22940 0.43 +1.9 2,376,018 - PGA1_c22940 0.43 +0.7 2,376,018 - PGA1_c22940 0.43 +0.7 2,376,058 + PGA1_c22940 0.47 -2.7 2,376,070 + PGA1_c22940 0.48 +1.6 2,376,070 + PGA1_c22940 0.48 +4.6 2,376,070 + PGA1_c22940 0.48 -1.6 2,376,070 + PGA1_c22940 0.48 +0.2 2,376,070 + PGA1_c22940 0.48 +1.2 2,376,078 - PGA1_c22940 0.49 +1.1 2,376,078 - PGA1_c22940 0.49 -0.6 2,376,078 - PGA1_c22940 0.49 +1.4 2,376,110 + PGA1_c22940 0.52 -2.9 2,376,110 + PGA1_c22940 0.52 +3.2 2,376,157 + PGA1_c22940 0.57 +1.4 2,376,157 + PGA1_c22940 0.57 +1.7 2,376,177 + PGA1_c22940 0.59 +1.4 2,376,189 + PGA1_c22940 0.60 +1.4 2,376,199 - PGA1_c22940 0.61 -1.7 2,376,241 + PGA1_c22940 0.65 +1.0 2,376,243 - PGA1_c22940 0.65 +2.3 2,376,304 + PGA1_c22940 0.71 +0.5
Or see this region's nucleotide sequence