Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01810 and PGA1_c01820 are separated by 80 nucleotides PGA1_c01820 and PGA1_c01830 are separated by 147 nucleotides PGA1_c01830 and PGA1_c01840 are separated by 593 nucleotides
PGA1_c01810: PGA1_c01810 - putative transcriptional regulator, GntR family, at 164,896 to 166,308
_c01810
PGA1_c01820: PGA1_c01820 - Predicted permeases, at 166,389 to 167,333
_c01820
PGA1_c01830: PGA1_c01830 - Uncharacterized conserved small protein, at 167,481 to 167,696
_c01830
PGA1_c01840: PGA1_c01840 - transmembrane ion channel-domain containing protein, at 168,290 to 168,802
_c01840
Position (kb)
166
167
168 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 165.432 kb on + strand, within PGA1_c01810 at 165.458 kb on - strand, within PGA1_c01810 at 165.458 kb on - strand, within PGA1_c01810 at 165.464 kb on + strand, within PGA1_c01810 at 165.498 kb on + strand, within PGA1_c01810 at 165.498 kb on + strand, within PGA1_c01810 at 165.565 kb on + strand, within PGA1_c01810 at 165.589 kb on - strand, within PGA1_c01810 at 165.622 kb on - strand, within PGA1_c01810 at 165.622 kb on - strand, within PGA1_c01810 at 165.714 kb on + strand, within PGA1_c01810 at 165.752 kb on + strand, within PGA1_c01810 at 165.760 kb on - strand, within PGA1_c01810 at 165.778 kb on + strand, within PGA1_c01810 at 165.778 kb on + strand, within PGA1_c01810 at 165.781 kb on - strand, within PGA1_c01810 at 165.786 kb on - strand, within PGA1_c01810 at 165.839 kb on + strand, within PGA1_c01810 at 165.934 kb on - strand, within PGA1_c01810 at 165.979 kb on - strand, within PGA1_c01810 at 166.008 kb on + strand, within PGA1_c01810 at 166.008 kb on + strand, within PGA1_c01810 at 166.016 kb on - strand, within PGA1_c01810 at 166.031 kb on + strand, within PGA1_c01810 at 166.041 kb on - strand, within PGA1_c01810 at 166.041 kb on - strand, within PGA1_c01810 at 166.082 kb on - strand, within PGA1_c01810 at 166.179 kb on + strand at 166.187 kb on - strand at 166.187 kb on - strand at 166.341 kb on - strand at 166.388 kb on - strand at 166.413 kb on - strand at 166.449 kb on + strand at 166.451 kb on + strand at 166.459 kb on - strand at 166.477 kb on + strand at 166.494 kb on - strand, within PGA1_c01820 at 166.513 kb on + strand, within PGA1_c01820 at 166.521 kb on - strand, within PGA1_c01820 at 166.521 kb on - strand, within PGA1_c01820 at 166.546 kb on - strand, within PGA1_c01820 at 166.555 kb on + strand, within PGA1_c01820 at 166.590 kb on - strand, within PGA1_c01820 at 166.592 kb on - strand, within PGA1_c01820 at 166.660 kb on + strand, within PGA1_c01820 at 166.686 kb on - strand, within PGA1_c01820 at 166.693 kb on + strand, within PGA1_c01820 at 166.693 kb on + strand, within PGA1_c01820 at 166.701 kb on - strand, within PGA1_c01820 at 166.701 kb on - strand, within PGA1_c01820 at 166.703 kb on + strand, within PGA1_c01820 at 166.739 kb on - strand, within PGA1_c01820 at 166.744 kb on - strand, within PGA1_c01820 at 166.744 kb on - strand, within PGA1_c01820 at 166.744 kb on - strand, within PGA1_c01820 at 166.758 kb on - strand, within PGA1_c01820 at 166.766 kb on - strand, within PGA1_c01820 at 166.766 kb on - strand, within PGA1_c01820 at 166.795 kb on + strand, within PGA1_c01820 at 166.821 kb on - strand, within PGA1_c01820 at 166.831 kb on - strand, within PGA1_c01820 at 166.835 kb on + strand, within PGA1_c01820 at 166.929 kb on - strand, within PGA1_c01820 at 166.942 kb on + strand, within PGA1_c01820 at 166.972 kb on - strand, within PGA1_c01820 at 166.979 kb on + strand, within PGA1_c01820 at 166.983 kb on + strand, within PGA1_c01820 at 166.996 kb on - strand, within PGA1_c01820 at 167.026 kb on + strand, within PGA1_c01820 at 167.040 kb on - strand, within PGA1_c01820 at 167.049 kb on + strand, within PGA1_c01820 at 167.063 kb on - strand, within PGA1_c01820 at 167.132 kb on + strand, within PGA1_c01820 at 167.132 kb on + strand, within PGA1_c01820 at 167.132 kb on + strand, within PGA1_c01820 at 167.140 kb on - strand, within PGA1_c01820 at 167.140 kb on - strand, within PGA1_c01820 at 167.140 kb on - strand, within PGA1_c01820 at 167.165 kb on + strand, within PGA1_c01820 at 167.191 kb on - strand, within PGA1_c01820 at 167.193 kb on + strand, within PGA1_c01820 at 167.196 kb on - strand, within PGA1_c01820 at 167.233 kb on + strand, within PGA1_c01820 at 167.233 kb on + strand, within PGA1_c01820 at 167.233 kb on + strand, within PGA1_c01820 at 167.301 kb on + strand at 167.301 kb on + strand at 167.301 kb on + strand at 167.301 kb on + strand at 167.301 kb on + strand at 167.301 kb on + strand at 167.309 kb on - strand at 167.309 kb on - strand at 167.309 kb on - strand at 167.309 kb on - strand at 167.319 kb on - strand at 167.353 kb on - strand at 167.366 kb on + strand at 167.366 kb on + strand at 167.471 kb on + strand at 167.487 kb on - strand at 167.528 kb on - strand, within PGA1_c01830 at 167.558 kb on + strand, within PGA1_c01830 at 167.593 kb on - strand, within PGA1_c01830 at 167.596 kb on + strand, within PGA1_c01830 at 167.623 kb on - strand, within PGA1_c01830 at 167.744 kb on + strand at 167.910 kb on + strand at 167.929 kb on + strand at 167.942 kb on - strand at 167.952 kb on + strand at 167.960 kb on - strand at 167.960 kb on - strand at 167.960 kb on - strand at 168.018 kb on - strand at 168.042 kb on + strand at 168.048 kb on + strand at 168.048 kb on + strand at 168.089 kb on - strand at 168.089 kb on - strand at 168.113 kb on + strand at 168.113 kb on + strand at 168.113 kb on + strand at 168.115 kb on + strand at 168.121 kb on - strand at 168.123 kb on - strand at 168.138 kb on - strand at 168.219 kb on + strand at 168.272 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 165,432 + PGA1_c01810 0.38 -1.9 165,458 - PGA1_c01810 0.40 +1.5 165,458 - PGA1_c01810 0.40 -0.7 165,464 + PGA1_c01810 0.40 -1.4 165,498 + PGA1_c01810 0.43 +0.3 165,498 + PGA1_c01810 0.43 -1.8 165,565 + PGA1_c01810 0.47 -2.4 165,589 - PGA1_c01810 0.49 +2.5 165,622 - PGA1_c01810 0.51 -1.8 165,622 - PGA1_c01810 0.51 -2.7 165,714 + PGA1_c01810 0.58 -1.1 165,752 + PGA1_c01810 0.61 +2.9 165,760 - PGA1_c01810 0.61 -0.7 165,778 + PGA1_c01810 0.62 +3.5 165,778 + PGA1_c01810 0.62 -2.6 165,781 - PGA1_c01810 0.63 +0.6 165,786 - PGA1_c01810 0.63 -2.0 165,839 + PGA1_c01810 0.67 -1.0 165,934 - PGA1_c01810 0.73 +1.0 165,979 - PGA1_c01810 0.77 -1.6 166,008 + PGA1_c01810 0.79 -0.4 166,008 + PGA1_c01810 0.79 -1.1 166,016 - PGA1_c01810 0.79 +2.0 166,031 + PGA1_c01810 0.80 +1.8 166,041 - PGA1_c01810 0.81 -1.4 166,041 - PGA1_c01810 0.81 -1.9 166,082 - PGA1_c01810 0.84 +2.9 166,179 + -1.0 166,187 - -0.9 166,187 - +1.9 166,341 - -1.8 166,388 - -1.5 166,413 - +1.9 166,449 + -1.9 166,451 + +1.6 166,459 - +2.4 166,477 + -1.8 166,494 - PGA1_c01820 0.11 +1.9 166,513 + PGA1_c01820 0.13 +1.1 166,521 - PGA1_c01820 0.14 -0.6 166,521 - PGA1_c01820 0.14 +2.9 166,546 - PGA1_c01820 0.17 -1.7 166,555 + PGA1_c01820 0.18 -0.9 166,590 - PGA1_c01820 0.21 +1.8 166,592 - PGA1_c01820 0.21 +3.6 166,660 + PGA1_c01820 0.29 +1.4 166,686 - PGA1_c01820 0.31 -3.0 166,693 + PGA1_c01820 0.32 +0.9 166,693 + PGA1_c01820 0.32 -1.3 166,701 - PGA1_c01820 0.33 +1.8 166,701 - PGA1_c01820 0.33 -1.5 166,703 + PGA1_c01820 0.33 -2.1 166,739 - PGA1_c01820 0.37 +1.9 166,744 - PGA1_c01820 0.38 +2.2 166,744 - PGA1_c01820 0.38 +1.0 166,744 - PGA1_c01820 0.38 -2.4 166,758 - PGA1_c01820 0.39 +1.9 166,766 - PGA1_c01820 0.40 -0.2 166,766 - PGA1_c01820 0.40 -1.8 166,795 + PGA1_c01820 0.43 -1.3 166,821 - PGA1_c01820 0.46 -2.0 166,831 - PGA1_c01820 0.47 +2.7 166,835 + PGA1_c01820 0.47 -1.2 166,929 - PGA1_c01820 0.57 +2.2 166,942 + PGA1_c01820 0.59 +1.3 166,972 - PGA1_c01820 0.62 -0.2 166,979 + PGA1_c01820 0.62 +0.8 166,983 + PGA1_c01820 0.63 +2.3 166,996 - PGA1_c01820 0.64 +4.3 167,026 + PGA1_c01820 0.67 +0.9 167,040 - PGA1_c01820 0.69 -2.0 167,049 + PGA1_c01820 0.70 -2.6 167,063 - PGA1_c01820 0.71 +1.3 167,132 + PGA1_c01820 0.79 -0.8 167,132 + PGA1_c01820 0.79 +1.4 167,132 + PGA1_c01820 0.79 +2.3 167,140 - PGA1_c01820 0.79 -1.2 167,140 - PGA1_c01820 0.79 -1.1 167,140 - PGA1_c01820 0.79 +0.7 167,165 + PGA1_c01820 0.82 +0.6 167,191 - PGA1_c01820 0.85 -2.8 167,193 + PGA1_c01820 0.85 -2.0 167,196 - PGA1_c01820 0.85 +2.3 167,233 + PGA1_c01820 0.89 +1.1 167,233 + PGA1_c01820 0.89 -2.4 167,233 + PGA1_c01820 0.89 +1.9 167,301 + -1.6 167,301 + +2.2 167,301 + -2.1 167,301 + +0.1 167,301 + +1.1 167,301 + +1.3 167,309 - +1.0 167,309 - -0.5 167,309 - -2.9 167,309 - -2.8 167,319 - -2.8 167,353 - +0.8 167,366 + -0.4 167,366 + -2.8 167,471 + +1.9 167,487 - -2.8 167,528 - PGA1_c01830 0.22 -1.3 167,558 + PGA1_c01830 0.36 -1.9 167,593 - PGA1_c01830 0.52 +1.7 167,596 + PGA1_c01830 0.53 -1.1 167,623 - PGA1_c01830 0.66 +1.6 167,744 + +3.8 167,910 + +2.3 167,929 + -1.8 167,942 - +2.2 167,952 + -1.5 167,960 - -0.9 167,960 - +1.6 167,960 - +2.0 168,018 - -2.7 168,042 + +2.1 168,048 + +1.2 168,048 + -0.3 168,089 - -2.0 168,089 - +0.3 168,113 + -0.8 168,113 + -2.4 168,113 + +3.5 168,115 + +1.0 168,121 - -3.0 168,123 - +1.5 168,138 - -2.0 168,219 + +0.2 168,272 + -1.2
Or see this region's nucleotide sequence