Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c16440 and PGA1_c16450 are separated by 86 nucleotides PGA1_c16450 and PGA1_c16460 overlap by 1 nucleotides PGA1_c16460 and PGA1_c16470 are separated by 67 nucleotides
PGA1_c16440: PGA1_c16440 - MFS transporter, at 1,704,296 to 1,705,522
_c16440
PGA1_c16450: PGA1_c16450 - Phytoene/squalene synthetase, at 1,705,609 to 1,706,388
_c16450
PGA1_c16460: PGA1_c16460 - 2-isopropylmalate synthase/homocitrate synthase-like protein, at 1,706,388 to 1,708,019
_c16460
PGA1_c16470: PGA1_c16470 - cysteinyl-tRNA synthetase CysS, at 1,708,087 to 1,709,484
_c16470
Position (kb)
1706
1707
1708
1709 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1705.398 kb on + strand, within PGA1_c16440 at 1705.415 kb on + strand at 1705.488 kb on - strand at 1705.498 kb on + strand at 1705.607 kb on + strand at 1705.613 kb on - strand at 1705.636 kb on + strand at 1705.649 kb on - strand at 1705.839 kb on + strand, within PGA1_c16450 at 1706.105 kb on + strand, within PGA1_c16450 at 1706.230 kb on + strand, within PGA1_c16450 at 1706.230 kb on + strand, within PGA1_c16450 at 1706.232 kb on - strand, within PGA1_c16450 at 1706.541 kb on + strand at 1706.601 kb on + strand, within PGA1_c16460 at 1706.630 kb on - strand, within PGA1_c16460 at 1706.649 kb on - strand, within PGA1_c16460 at 1706.743 kb on + strand, within PGA1_c16460 at 1706.760 kb on - strand, within PGA1_c16460 at 1706.791 kb on + strand, within PGA1_c16460 at 1706.799 kb on - strand, within PGA1_c16460 at 1706.799 kb on - strand, within PGA1_c16460 at 1707.106 kb on + strand, within PGA1_c16460 at 1707.261 kb on - strand, within PGA1_c16460 at 1707.313 kb on + strand, within PGA1_c16460 at 1707.316 kb on - strand, within PGA1_c16460 at 1707.336 kb on - strand, within PGA1_c16460 at 1707.559 kb on + strand, within PGA1_c16460 at 1707.559 kb on + strand, within PGA1_c16460 at 1707.612 kb on + strand, within PGA1_c16460 at 1707.733 kb on - strand, within PGA1_c16460 at 1707.899 kb on + strand at 1707.899 kb on + strand at 1707.899 kb on + strand at 1707.907 kb on - strand at 1707.907 kb on - strand at 1707.907 kb on - strand at 1707.958 kb on + strand at 1707.958 kb on + strand at 1707.958 kb on + strand at 1707.958 kb on + strand at 1707.964 kb on - strand at 1707.966 kb on - strand at 1707.966 kb on - strand at 1707.966 kb on - strand at 1707.966 kb on - strand at 1707.988 kb on + strand at 1707.996 kb on - strand at 1708.034 kb on - strand at 1708.034 kb on - strand at 1708.041 kb on + strand at 1708.052 kb on + strand at 1708.052 kb on + strand at 1708.059 kb on + strand at 1708.060 kb on - strand at 1708.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 1,705,398 + PGA1_c16440 0.90 +1.5 1,705,415 + -2.0 1,705,488 - +2.5 1,705,498 + +0.8 1,705,607 + +0.9 1,705,613 - +0.4 1,705,636 + +1.1 1,705,649 - +1.8 1,705,839 + PGA1_c16450 0.29 +4.7 1,706,105 + PGA1_c16450 0.64 +2.5 1,706,230 + PGA1_c16450 0.80 -0.0 1,706,230 + PGA1_c16450 0.80 +1.7 1,706,232 - PGA1_c16450 0.80 -1.5 1,706,541 + +0.8 1,706,601 + PGA1_c16460 0.13 +3.2 1,706,630 - PGA1_c16460 0.15 -1.4 1,706,649 - PGA1_c16460 0.16 -1.0 1,706,743 + PGA1_c16460 0.22 +3.8 1,706,760 - PGA1_c16460 0.23 +1.5 1,706,791 + PGA1_c16460 0.25 +2.5 1,706,799 - PGA1_c16460 0.25 +1.9 1,706,799 - PGA1_c16460 0.25 +2.3 1,707,106 + PGA1_c16460 0.44 +2.1 1,707,261 - PGA1_c16460 0.53 -0.3 1,707,313 + PGA1_c16460 0.57 +3.0 1,707,316 - PGA1_c16460 0.57 +2.8 1,707,336 - PGA1_c16460 0.58 +0.5 1,707,559 + PGA1_c16460 0.72 +2.0 1,707,559 + PGA1_c16460 0.72 +2.0 1,707,612 + PGA1_c16460 0.75 +2.1 1,707,733 - PGA1_c16460 0.82 +2.7 1,707,899 + +3.6 1,707,899 + +3.4 1,707,899 + +2.0 1,707,907 - +2.4 1,707,907 - +1.5 1,707,907 - -2.1 1,707,958 + -2.7 1,707,958 + -2.2 1,707,958 + +1.2 1,707,958 + -0.5 1,707,964 - +2.0 1,707,966 - -0.1 1,707,966 - -1.9 1,707,966 - -1.4 1,707,966 - +1.4 1,707,988 + +1.5 1,707,996 - +3.9 1,708,034 - -2.3 1,708,034 - +2.4 1,708,041 + +3.6 1,708,052 + +1.3 1,708,052 + -2.1 1,708,059 + -1.9 1,708,060 - +2.9 1,708,067 - -0.8
Or see this region's nucleotide sequence