Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c14120 and PGA1_c14130 are separated by 344 nucleotides PGA1_c14130 and PGA1_c14140 are separated by 295 nucleotides PGA1_c14140 and PGA1_c14150 are separated by 47 nucleotides
PGA1_c14120: PGA1_c14120 - single-stranded DNA-binding protein Ssb, at 1,464,821 to 1,465,363
_c14120
PGA1_c14130: PGA1_c14130 - transglycolase-like protein, at 1,465,708 to 1,466,292
_c14130
PGA1_c14140: PGA1_c14140 - hypothetical protein, at 1,466,588 to 1,466,962
_c14140
PGA1_c14150: PGA1_c14150 - glutathione import ATP-binding protein GsiA, at 1,467,010 to 1,469,097
_c14150
Position (kb)
1465
1466
1467 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1464.747 kb on + strand at 1464.753 kb on + strand at 1464.753 kb on + strand at 1464.755 kb on - strand at 1464.761 kb on - strand at 1464.766 kb on + strand at 1464.766 kb on + strand at 1464.766 kb on + strand at 1464.766 kb on - strand at 1464.768 kb on + strand at 1464.768 kb on + strand at 1464.768 kb on + strand at 1464.774 kb on - strand at 1464.776 kb on - strand at 1464.776 kb on - strand at 1464.776 kb on - strand at 1464.776 kb on - strand at 1464.790 kb on + strand at 1465.575 kb on + strand at 1465.606 kb on + strand at 1465.619 kb on + strand at 1465.675 kb on - strand at 1465.675 kb on - strand at 1465.675 kb on - strand at 1465.675 kb on - strand at 1465.675 kb on - strand at 1465.718 kb on - strand at 1465.735 kb on + strand at 1465.735 kb on - strand at 1465.737 kb on + strand at 1465.742 kb on + strand at 1465.784 kb on - strand, within PGA1_c14130 at 1465.835 kb on - strand, within PGA1_c14130 at 1465.838 kb on + strand, within PGA1_c14130 at 1465.846 kb on - strand, within PGA1_c14130 at 1465.846 kb on - strand, within PGA1_c14130 at 1465.872 kb on + strand, within PGA1_c14130 at 1465.907 kb on + strand, within PGA1_c14130 at 1465.911 kb on + strand, within PGA1_c14130 at 1465.911 kb on + strand, within PGA1_c14130 at 1465.929 kb on - strand, within PGA1_c14130 at 1465.958 kb on + strand, within PGA1_c14130 at 1465.995 kb on - strand, within PGA1_c14130 at 1466.029 kb on - strand, within PGA1_c14130 at 1466.099 kb on - strand, within PGA1_c14130 at 1466.169 kb on + strand, within PGA1_c14130 at 1466.285 kb on - strand at 1466.288 kb on + strand at 1466.288 kb on + strand at 1466.288 kb on + strand at 1466.288 kb on + strand at 1466.296 kb on - strand at 1466.296 kb on - strand at 1466.326 kb on + strand at 1466.345 kb on - strand at 1466.421 kb on + strand at 1466.429 kb on - strand at 1466.449 kb on + strand at 1466.476 kb on + strand at 1466.476 kb on + strand at 1466.476 kb on + strand at 1466.476 kb on + strand at 1466.498 kb on + strand at 1466.535 kb on - strand at 1466.538 kb on + strand at 1466.538 kb on + strand at 1466.558 kb on + strand at 1466.566 kb on - strand at 1466.566 kb on - strand at 1466.613 kb on + strand at 1466.621 kb on - strand at 1466.645 kb on - strand, within PGA1_c14140 at 1466.670 kb on + strand, within PGA1_c14140 at 1466.751 kb on + strand, within PGA1_c14140 at 1466.751 kb on + strand, within PGA1_c14140 at 1466.759 kb on - strand, within PGA1_c14140 at 1466.818 kb on + strand, within PGA1_c14140 at 1466.964 kb on - strand at 1466.989 kb on - strand at 1467.094 kb on - strand at 1467.114 kb on + strand at 1467.125 kb on - strand at 1467.131 kb on - strand at 1467.224 kb on + strand, within PGA1_c14150 at 1467.273 kb on - strand, within PGA1_c14150 at 1467.281 kb on + strand, within PGA1_c14150 at 1467.281 kb on + strand, within PGA1_c14150 at 1467.281 kb on + strand, within PGA1_c14150 at 1467.281 kb on + strand, within PGA1_c14150 at 1467.281 kb on + strand, within PGA1_c14150 at 1467.281 kb on + strand, within PGA1_c14150 at 1467.281 kb on + strand, within PGA1_c14150 at 1467.289 kb on - strand, within PGA1_c14150
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 1,464,747 + +2.0 1,464,753 + -1.5 1,464,753 + -1.8 1,464,755 - -0.5 1,464,761 - +0.1 1,464,766 + -0.6 1,464,766 + +3.1 1,464,766 + -3.0 1,464,766 - +0.8 1,464,768 + +0.8 1,464,768 + -2.6 1,464,768 + -0.4 1,464,774 - -1.5 1,464,776 - -2.0 1,464,776 - -1.3 1,464,776 - -2.5 1,464,776 - +1.5 1,464,790 + -0.0 1,465,575 + -1.3 1,465,606 + -2.9 1,465,619 + -0.5 1,465,675 - -1.5 1,465,675 - -1.5 1,465,675 - -1.8 1,465,675 - -1.5 1,465,675 - -2.8 1,465,718 - -1.6 1,465,735 + -1.0 1,465,735 - -1.9 1,465,737 + -2.4 1,465,742 + +0.7 1,465,784 - PGA1_c14130 0.13 -1.1 1,465,835 - PGA1_c14130 0.22 -2.2 1,465,838 + PGA1_c14130 0.22 -2.2 1,465,846 - PGA1_c14130 0.24 -1.9 1,465,846 - PGA1_c14130 0.24 -1.5 1,465,872 + PGA1_c14130 0.28 +0.3 1,465,907 + PGA1_c14130 0.34 -1.5 1,465,911 + PGA1_c14130 0.35 -1.4 1,465,911 + PGA1_c14130 0.35 -0.8 1,465,929 - PGA1_c14130 0.38 -1.2 1,465,958 + PGA1_c14130 0.43 -1.3 1,465,995 - PGA1_c14130 0.49 -2.3 1,466,029 - PGA1_c14130 0.55 -1.1 1,466,099 - PGA1_c14130 0.67 -1.9 1,466,169 + PGA1_c14130 0.79 -2.5 1,466,285 - -1.8 1,466,288 + -2.8 1,466,288 + +0.3 1,466,288 + +1.5 1,466,288 + +1.0 1,466,296 - +2.2 1,466,296 - +0.7 1,466,326 + +1.4 1,466,345 - +1.8 1,466,421 + +2.0 1,466,429 - +0.4 1,466,449 + -2.2 1,466,476 + -0.9 1,466,476 + +1.6 1,466,476 + -0.9 1,466,476 + +1.0 1,466,498 + -1.5 1,466,535 - -2.1 1,466,538 + +2.9 1,466,538 + +0.4 1,466,558 + +3.2 1,466,566 - +1.8 1,466,566 - -2.6 1,466,613 + +1.0 1,466,621 - +2.4 1,466,645 - PGA1_c14140 0.15 +3.4 1,466,670 + PGA1_c14140 0.22 -1.1 1,466,751 + PGA1_c14140 0.43 -2.3 1,466,751 + PGA1_c14140 0.43 -1.3 1,466,759 - PGA1_c14140 0.46 +3.6 1,466,818 + PGA1_c14140 0.61 -0.1 1,466,964 - +1.6 1,466,989 - -2.2 1,467,094 - +1.8 1,467,114 + +1.6 1,467,125 - +2.3 1,467,131 - -0.9 1,467,224 + PGA1_c14150 0.10 -3.4 1,467,273 - PGA1_c14150 0.13 -1.5 1,467,281 + PGA1_c14150 0.13 -2.1 1,467,281 + PGA1_c14150 0.13 -2.1 1,467,281 + PGA1_c14150 0.13 -2.5 1,467,281 + PGA1_c14150 0.13 -2.3 1,467,281 + PGA1_c14150 0.13 +1.0 1,467,281 + PGA1_c14150 0.13 -0.8 1,467,281 + PGA1_c14150 0.13 +3.5 1,467,289 - PGA1_c14150 0.13 +1.3
Or see this region's nucleotide sequence