Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12020 and PGA1_c12030 are separated by 23 nucleotides PGA1_c12030 and PGA1_c12050 are separated by 10 nucleotides PGA1_c12050 and PGA1_c12060 are separated by 281 nucleotides
PGA1_c12020: PGA1_c12020 - fatty acid oxidation complex FadJ subunit alpha, at 1,249,462 to 1,251,663
_c12020
PGA1_c12030: PGA1_c12030 - 3-ketoacyl-CoA thiolase FadA, at 1,251,687 to 1,252,898
_c12030
PGA1_c12050: PGA1_c12050 - putative glutathione S-transferase, at 1,252,909 to 1,253,547
_c12050
PGA1_c12060: PGA1_c12060 - acyl-CoA dehydrogenase, at 1,253,829 to 1,254,980
_c12060
Position (kb)
1251
1252
1253 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1250.793 kb on - strand, within PGA1_c12020 at 1250.826 kb on - strand, within PGA1_c12020 at 1250.828 kb on + strand, within PGA1_c12020 at 1250.844 kb on - strand, within PGA1_c12020 at 1250.855 kb on - strand, within PGA1_c12020 at 1250.944 kb on - strand, within PGA1_c12020 at 1250.959 kb on - strand, within PGA1_c12020 at 1250.993 kb on + strand, within PGA1_c12020 at 1251.071 kb on - strand, within PGA1_c12020 at 1251.107 kb on - strand, within PGA1_c12020 at 1251.138 kb on - strand, within PGA1_c12020 at 1251.253 kb on + strand, within PGA1_c12020 at 1251.307 kb on - strand, within PGA1_c12020 at 1251.376 kb on - strand, within PGA1_c12020 at 1251.401 kb on + strand, within PGA1_c12020 at 1251.409 kb on - strand, within PGA1_c12020 at 1251.411 kb on + strand, within PGA1_c12020 at 1251.411 kb on + strand, within PGA1_c12020 at 1251.460 kb on + strand at 1251.468 kb on - strand at 1251.492 kb on + strand at 1251.511 kb on + strand at 1251.519 kb on - strand at 1251.614 kb on - strand at 1251.693 kb on - strand at 1251.751 kb on + strand at 1251.777 kb on + strand at 1251.802 kb on - strand at 1251.844 kb on + strand, within PGA1_c12030 at 1251.869 kb on - strand, within PGA1_c12030 at 1251.874 kb on + strand, within PGA1_c12030 at 1251.914 kb on - strand, within PGA1_c12030 at 1251.920 kb on - strand, within PGA1_c12030 at 1251.977 kb on - strand, within PGA1_c12030 at 1251.986 kb on + strand, within PGA1_c12030 at 1251.999 kb on - strand, within PGA1_c12030 at 1252.036 kb on + strand, within PGA1_c12030 at 1252.044 kb on - strand, within PGA1_c12030 at 1252.044 kb on - strand, within PGA1_c12030 at 1252.131 kb on - strand, within PGA1_c12030 at 1252.150 kb on + strand, within PGA1_c12030 at 1252.275 kb on + strand, within PGA1_c12030 at 1252.307 kb on - strand, within PGA1_c12030 at 1252.324 kb on + strand, within PGA1_c12030 at 1252.363 kb on + strand, within PGA1_c12030 at 1252.427 kb on - strand, within PGA1_c12030 at 1252.447 kb on + strand, within PGA1_c12030 at 1252.566 kb on - strand, within PGA1_c12030 at 1252.721 kb on + strand, within PGA1_c12030 at 1252.842 kb on + strand at 1252.881 kb on + strand at 1252.881 kb on + strand at 1252.881 kb on + strand at 1252.881 kb on - strand at 1252.907 kb on + strand at 1252.907 kb on + strand at 1252.915 kb on - strand at 1252.943 kb on - strand at 1253.030 kb on + strand, within PGA1_c12050 at 1253.031 kb on + strand, within PGA1_c12050 at 1253.039 kb on - strand, within PGA1_c12050 at 1253.044 kb on - strand, within PGA1_c12050 at 1253.044 kb on - strand, within PGA1_c12050 at 1253.059 kb on + strand, within PGA1_c12050 at 1253.067 kb on - strand, within PGA1_c12050 at 1253.067 kb on - strand, within PGA1_c12050 at 1253.067 kb on - strand, within PGA1_c12050 at 1253.067 kb on - strand, within PGA1_c12050 at 1253.079 kb on + strand, within PGA1_c12050 at 1253.161 kb on + strand, within PGA1_c12050 at 1253.170 kb on - strand, within PGA1_c12050 at 1253.238 kb on - strand, within PGA1_c12050 at 1253.246 kb on - strand, within PGA1_c12050 at 1253.257 kb on - strand, within PGA1_c12050 at 1253.263 kb on + strand, within PGA1_c12050 at 1253.315 kb on - strand, within PGA1_c12050 at 1253.356 kb on + strand, within PGA1_c12050 at 1253.364 kb on - strand, within PGA1_c12050 at 1253.371 kb on + strand, within PGA1_c12050 at 1253.506 kb on - strand at 1253.545 kb on + strand at 1253.553 kb on - strand at 1253.589 kb on + strand at 1253.589 kb on + strand at 1253.589 kb on + strand at 1253.589 kb on + strand at 1253.598 kb on - strand at 1253.629 kb on + strand at 1253.677 kb on - strand at 1253.743 kb on - strand at 1253.750 kb on + strand at 1253.758 kb on - strand at 1253.828 kb on + strand at 1253.830 kb on + strand at 1253.883 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 1,250,793 - PGA1_c12020 0.60 +2.9 1,250,826 - PGA1_c12020 0.62 -0.5 1,250,828 + PGA1_c12020 0.62 +1.2 1,250,844 - PGA1_c12020 0.63 -3.4 1,250,855 - PGA1_c12020 0.63 -0.4 1,250,944 - PGA1_c12020 0.67 -0.8 1,250,959 - PGA1_c12020 0.68 -2.2 1,250,993 + PGA1_c12020 0.70 +0.1 1,251,071 - PGA1_c12020 0.73 -0.2 1,251,107 - PGA1_c12020 0.75 +0.6 1,251,138 - PGA1_c12020 0.76 +2.3 1,251,253 + PGA1_c12020 0.81 +3.3 1,251,307 - PGA1_c12020 0.84 +2.0 1,251,376 - PGA1_c12020 0.87 +0.5 1,251,401 + PGA1_c12020 0.88 +0.5 1,251,409 - PGA1_c12020 0.88 -1.7 1,251,411 + PGA1_c12020 0.89 +2.3 1,251,411 + PGA1_c12020 0.89 +0.4 1,251,460 + +1.0 1,251,468 - -0.8 1,251,492 + -2.7 1,251,511 + +1.7 1,251,519 - -2.7 1,251,614 - +3.2 1,251,693 - +0.2 1,251,751 + -1.7 1,251,777 + -0.9 1,251,802 - -1.8 1,251,844 + PGA1_c12030 0.13 +1.1 1,251,869 - PGA1_c12030 0.15 -1.8 1,251,874 + PGA1_c12030 0.15 +1.6 1,251,914 - PGA1_c12030 0.19 +1.7 1,251,920 - PGA1_c12030 0.19 +1.0 1,251,977 - PGA1_c12030 0.24 -2.1 1,251,986 + PGA1_c12030 0.25 -0.1 1,251,999 - PGA1_c12030 0.26 -0.7 1,252,036 + PGA1_c12030 0.29 +1.2 1,252,044 - PGA1_c12030 0.29 +0.9 1,252,044 - PGA1_c12030 0.29 +2.5 1,252,131 - PGA1_c12030 0.37 +0.1 1,252,150 + PGA1_c12030 0.38 +2.0 1,252,275 + PGA1_c12030 0.49 +0.1 1,252,307 - PGA1_c12030 0.51 +0.5 1,252,324 + PGA1_c12030 0.53 -2.0 1,252,363 + PGA1_c12030 0.56 -2.0 1,252,427 - PGA1_c12030 0.61 -1.4 1,252,447 + PGA1_c12030 0.63 -2.7 1,252,566 - PGA1_c12030 0.73 +1.4 1,252,721 + PGA1_c12030 0.85 +0.9 1,252,842 + +2.9 1,252,881 + +1.6 1,252,881 + -1.0 1,252,881 + +1.9 1,252,881 - +2.2 1,252,907 + +3.1 1,252,907 + +3.0 1,252,915 - +2.9 1,252,943 - -2.3 1,253,030 + PGA1_c12050 0.19 +2.1 1,253,031 + PGA1_c12050 0.19 -2.8 1,253,039 - PGA1_c12050 0.20 +2.5 1,253,044 - PGA1_c12050 0.21 -1.7 1,253,044 - PGA1_c12050 0.21 +1.4 1,253,059 + PGA1_c12050 0.23 +1.0 1,253,067 - PGA1_c12050 0.25 +1.9 1,253,067 - PGA1_c12050 0.25 -0.7 1,253,067 - PGA1_c12050 0.25 -1.4 1,253,067 - PGA1_c12050 0.25 +1.6 1,253,079 + PGA1_c12050 0.27 -1.2 1,253,161 + PGA1_c12050 0.39 -0.4 1,253,170 - PGA1_c12050 0.41 -0.8 1,253,238 - PGA1_c12050 0.51 -2.2 1,253,246 - PGA1_c12050 0.53 +1.4 1,253,257 - PGA1_c12050 0.54 -1.4 1,253,263 + PGA1_c12050 0.55 -1.3 1,253,315 - PGA1_c12050 0.64 -0.6 1,253,356 + PGA1_c12050 0.70 +1.1 1,253,364 - PGA1_c12050 0.71 -0.2 1,253,371 + PGA1_c12050 0.72 -1.1 1,253,506 - -0.6 1,253,545 + +3.6 1,253,553 - -1.7 1,253,589 + -1.6 1,253,589 + -1.1 1,253,589 + +0.5 1,253,589 + -1.8 1,253,598 - -1.2 1,253,629 + -0.4 1,253,677 - -1.0 1,253,743 - -2.4 1,253,750 + +1.9 1,253,758 - +1.1 1,253,828 + -2.0 1,253,830 + +2.3 1,253,883 - -2.8
Or see this region's nucleotide sequence