Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11260 and PGA1_c11270 are separated by 87 nucleotides PGA1_c11270 and PGA1_c11280 are separated by 211 nucleotides PGA1_c11280 and PGA1_c11290 are separated by 87 nucleotides
PGA1_c11260: PGA1_c11260 - Predicted metal-binding, possibly nucleic acid-binding protein, at 1,165,061 to 1,165,606
_c11260
PGA1_c11270: PGA1_c11270 - Small protein A (tmRNA-binding), at 1,165,694 to 1,166,161
_c11270
PGA1_c11280: PGA1_c11280 - Putative hemolysin, at 1,166,373 to 1,167,236
_c11280
PGA1_c11290: PGA1_c11290 - putative holdfast attachment protein C, at 1,167,324 to 1,169,111
_c11290
Position (kb)
1166
1167
1168 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1165.695 kb on - strand at 1166.174 kb on + strand at 1166.182 kb on - strand at 1166.192 kb on + strand at 1166.192 kb on + strand at 1166.200 kb on - strand at 1166.244 kb on - strand at 1166.249 kb on - strand at 1166.333 kb on + strand at 1166.354 kb on + strand at 1166.354 kb on + strand at 1166.359 kb on + strand at 1166.364 kb on + strand at 1166.364 kb on + strand at 1166.367 kb on - strand at 1166.372 kb on - strand at 1166.429 kb on - strand at 1166.436 kb on - strand at 1166.436 kb on - strand at 1166.437 kb on + strand at 1166.444 kb on + strand at 1166.552 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.626 kb on + strand, within PGA1_c11280 at 1166.638 kb on - strand, within PGA1_c11280 at 1166.689 kb on + strand, within PGA1_c11280 at 1166.732 kb on + strand, within PGA1_c11280 at 1166.740 kb on - strand, within PGA1_c11280 at 1166.740 kb on - strand, within PGA1_c11280 at 1166.740 kb on - strand, within PGA1_c11280 at 1166.749 kb on + strand, within PGA1_c11280 at 1166.835 kb on + strand, within PGA1_c11280 at 1166.841 kb on - strand, within PGA1_c11280 at 1166.849 kb on + strand, within PGA1_c11280 at 1166.851 kb on - strand, within PGA1_c11280 at 1166.903 kb on - strand, within PGA1_c11280 at 1166.931 kb on - strand, within PGA1_c11280 at 1166.938 kb on - strand, within PGA1_c11280 at 1166.978 kb on + strand, within PGA1_c11280 at 1166.995 kb on + strand, within PGA1_c11280 at 1167.042 kb on - strand, within PGA1_c11280 at 1167.243 kb on - strand at 1167.270 kb on - strand at 1167.270 kb on - strand at 1167.291 kb on - strand at 1167.392 kb on - strand at 1167.418 kb on - strand at 1167.443 kb on + strand at 1167.443 kb on + strand at 1167.460 kb on + strand at 1167.464 kb on + strand at 1167.464 kb on + strand at 1167.473 kb on + strand at 1167.492 kb on - strand at 1167.501 kb on + strand at 1167.508 kb on - strand, within PGA1_c11290 at 1167.684 kb on + strand, within PGA1_c11290 at 1167.689 kb on + strand, within PGA1_c11290 at 1167.695 kb on + strand, within PGA1_c11290 at 1167.810 kb on - strand, within PGA1_c11290 at 1167.810 kb on - strand, within PGA1_c11290 at 1167.810 kb on - strand, within PGA1_c11290 at 1167.840 kb on + strand, within PGA1_c11290 at 1167.840 kb on + strand, within PGA1_c11290 at 1167.840 kb on + strand, within PGA1_c11290 at 1167.840 kb on + strand, within PGA1_c11290 at 1167.848 kb on - strand, within PGA1_c11290 at 1167.848 kb on - strand, within PGA1_c11290 at 1167.862 kb on + strand, within PGA1_c11290 at 1167.868 kb on + strand, within PGA1_c11290 at 1167.907 kb on + strand, within PGA1_c11290 at 1167.951 kb on - strand, within PGA1_c11290 at 1167.964 kb on - strand, within PGA1_c11290 at 1167.968 kb on - strand, within PGA1_c11290 at 1167.999 kb on + strand, within PGA1_c11290 at 1168.082 kb on + strand, within PGA1_c11290 at 1168.089 kb on + strand, within PGA1_c11290 at 1168.090 kb on - strand, within PGA1_c11290 at 1168.090 kb on - strand, within PGA1_c11290 at 1168.094 kb on + strand, within PGA1_c11290 at 1168.102 kb on - strand, within PGA1_c11290 at 1168.141 kb on + strand, within PGA1_c11290
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 1,165,695 - -2.0 1,166,174 + +0.6 1,166,182 - -1.3 1,166,192 + +2.1 1,166,192 + +2.3 1,166,200 - +0.5 1,166,244 - -3.3 1,166,249 - -0.4 1,166,333 + -1.6 1,166,354 + +4.0 1,166,354 + -1.4 1,166,359 + -1.5 1,166,364 + -0.2 1,166,364 + -1.3 1,166,367 - -2.5 1,166,372 - +2.9 1,166,429 - +1.4 1,166,436 - +0.4 1,166,436 - -1.9 1,166,437 + +1.8 1,166,444 + +0.5 1,166,552 + PGA1_c11280 0.21 +3.1 1,166,575 + PGA1_c11280 0.23 -2.4 1,166,575 + PGA1_c11280 0.23 +2.6 1,166,575 + PGA1_c11280 0.23 -0.5 1,166,575 + PGA1_c11280 0.23 +1.1 1,166,626 + PGA1_c11280 0.29 -2.1 1,166,638 - PGA1_c11280 0.31 -1.6 1,166,689 + PGA1_c11280 0.37 +2.4 1,166,732 + PGA1_c11280 0.42 +0.9 1,166,740 - PGA1_c11280 0.42 -1.8 1,166,740 - PGA1_c11280 0.42 +2.0 1,166,740 - PGA1_c11280 0.42 +2.4 1,166,749 + PGA1_c11280 0.44 +2.2 1,166,835 + PGA1_c11280 0.53 -2.4 1,166,841 - PGA1_c11280 0.54 +2.7 1,166,849 + PGA1_c11280 0.55 +2.6 1,166,851 - PGA1_c11280 0.55 +1.5 1,166,903 - PGA1_c11280 0.61 +2.5 1,166,931 - PGA1_c11280 0.65 +2.8 1,166,938 - PGA1_c11280 0.65 -1.5 1,166,978 + PGA1_c11280 0.70 +2.4 1,166,995 + PGA1_c11280 0.72 +2.1 1,167,042 - PGA1_c11280 0.77 +3.3 1,167,243 - +2.1 1,167,270 - +2.0 1,167,270 - -1.2 1,167,291 - +1.5 1,167,392 - +1.5 1,167,418 - +0.8 1,167,443 + +1.7 1,167,443 + +2.2 1,167,460 + -0.0 1,167,464 + -1.6 1,167,464 + -2.6 1,167,473 + +0.7 1,167,492 - -0.2 1,167,501 + +1.3 1,167,508 - PGA1_c11290 0.10 +1.9 1,167,684 + PGA1_c11290 0.20 -1.4 1,167,689 + PGA1_c11290 0.20 -1.4 1,167,695 + PGA1_c11290 0.21 -2.4 1,167,810 - PGA1_c11290 0.27 -1.5 1,167,810 - PGA1_c11290 0.27 -2.6 1,167,810 - PGA1_c11290 0.27 -1.8 1,167,840 + PGA1_c11290 0.29 -1.4 1,167,840 + PGA1_c11290 0.29 +1.9 1,167,840 + PGA1_c11290 0.29 +1.4 1,167,840 + PGA1_c11290 0.29 +1.5 1,167,848 - PGA1_c11290 0.29 -3.3 1,167,848 - PGA1_c11290 0.29 +2.8 1,167,862 + PGA1_c11290 0.30 -1.7 1,167,868 + PGA1_c11290 0.30 -1.1 1,167,907 + PGA1_c11290 0.33 +1.5 1,167,951 - PGA1_c11290 0.35 +2.3 1,167,964 - PGA1_c11290 0.36 +0.8 1,167,968 - PGA1_c11290 0.36 -1.5 1,167,999 + PGA1_c11290 0.38 -1.6 1,168,082 + PGA1_c11290 0.42 +2.2 1,168,089 + PGA1_c11290 0.43 -1.9 1,168,090 - PGA1_c11290 0.43 -1.6 1,168,090 - PGA1_c11290 0.43 -1.2 1,168,094 + PGA1_c11290 0.43 +1.7 1,168,102 - PGA1_c11290 0.44 -1.5 1,168,141 + PGA1_c11290 0.46 -1.8
Or see this region's nucleotide sequence