Experiment: m.b. Polymyxin B sulfate 0.0032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01110 and PGA1_c01120 are separated by 146 nucleotides PGA1_c01120 and PGA1_c01130 are separated by 58 nucleotides
PGA1_c01110: PGA1_c01110 - acyl-CoA dehydrogenase, at 102,983 to 104,668
_c01110
PGA1_c01120: PGA1_c01120 - sulfite exporter tauE/safE-like protein, at 104,815 to 105,603
_c01120
PGA1_c01130: PGA1_c01130 - putative cation efflux pump, at 105,662 to 106,561
_c01130
Position (kb)
104
105
106 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 103.843 kb on + strand, within PGA1_c01110 at 103.928 kb on - strand, within PGA1_c01110 at 103.928 kb on - strand, within PGA1_c01110 at 103.930 kb on + strand, within PGA1_c01110 at 103.946 kb on + strand, within PGA1_c01110 at 103.954 kb on - strand, within PGA1_c01110 at 103.954 kb on - strand, within PGA1_c01110 at 103.954 kb on - strand, within PGA1_c01110 at 103.961 kb on - strand, within PGA1_c01110 at 103.961 kb on - strand, within PGA1_c01110 at 103.961 kb on - strand, within PGA1_c01110 at 103.961 kb on - strand, within PGA1_c01110 at 103.963 kb on + strand, within PGA1_c01110 at 103.963 kb on + strand, within PGA1_c01110 at 103.963 kb on + strand, within PGA1_c01110 at 103.963 kb on + strand, within PGA1_c01110 at 104.052 kb on + strand, within PGA1_c01110 at 104.053 kb on - strand, within PGA1_c01110 at 104.080 kb on + strand, within PGA1_c01110 at 104.151 kb on - strand, within PGA1_c01110 at 104.151 kb on - strand, within PGA1_c01110 at 104.174 kb on - strand, within PGA1_c01110 at 104.174 kb on - strand, within PGA1_c01110 at 104.174 kb on - strand, within PGA1_c01110 at 104.195 kb on - strand, within PGA1_c01110 at 104.223 kb on - strand, within PGA1_c01110 at 104.240 kb on + strand, within PGA1_c01110 at 104.245 kb on + strand, within PGA1_c01110 at 104.314 kb on - strand, within PGA1_c01110 at 104.334 kb on + strand, within PGA1_c01110 at 104.342 kb on - strand, within PGA1_c01110 at 104.409 kb on + strand, within PGA1_c01110 at 104.409 kb on + strand, within PGA1_c01110 at 104.417 kb on - strand, within PGA1_c01110 at 104.417 kb on - strand, within PGA1_c01110 at 104.417 kb on - strand, within PGA1_c01110 at 104.417 kb on - strand, within PGA1_c01110 at 104.417 kb on - strand, within PGA1_c01110 at 104.419 kb on + strand, within PGA1_c01110 at 104.419 kb on - strand, within PGA1_c01110 at 104.454 kb on - strand, within PGA1_c01110 at 104.454 kb on - strand, within PGA1_c01110 at 104.456 kb on - strand, within PGA1_c01110 at 104.474 kb on - strand, within PGA1_c01110 at 104.497 kb on - strand, within PGA1_c01110 at 104.527 kb on + strand at 104.647 kb on + strand at 104.647 kb on + strand at 104.671 kb on + strand at 104.679 kb on + strand at 104.806 kb on + strand at 104.806 kb on + strand at 104.811 kb on + strand at 104.814 kb on - strand at 104.849 kb on + strand at 104.854 kb on + strand at 104.854 kb on + strand at 104.854 kb on + strand at 104.856 kb on + strand at 104.862 kb on - strand at 104.862 kb on - strand at 104.862 kb on - strand at 104.862 kb on - strand at 104.864 kb on + strand at 104.864 kb on + strand at 104.864 kb on + strand at 104.864 kb on - strand at 104.872 kb on - strand at 104.883 kb on - strand at 104.883 kb on - strand at 104.952 kb on - strand, within PGA1_c01120 at 104.957 kb on + strand, within PGA1_c01120 at 104.962 kb on + strand, within PGA1_c01120 at 104.965 kb on - strand, within PGA1_c01120 at 105.008 kb on - strand, within PGA1_c01120 at 105.036 kb on - strand, within PGA1_c01120 at 105.117 kb on + strand, within PGA1_c01120 at 105.117 kb on + strand, within PGA1_c01120 at 105.122 kb on + strand, within PGA1_c01120 at 105.130 kb on - strand, within PGA1_c01120 at 105.160 kb on - strand, within PGA1_c01120 at 105.297 kb on + strand, within PGA1_c01120 at 105.340 kb on - strand, within PGA1_c01120 at 105.357 kb on + strand, within PGA1_c01120 at 105.405 kb on - strand, within PGA1_c01120 at 105.414 kb on + strand, within PGA1_c01120 at 105.424 kb on + strand, within PGA1_c01120 at 105.424 kb on + strand, within PGA1_c01120 at 105.424 kb on + strand, within PGA1_c01120 at 105.427 kb on - strand, within PGA1_c01120 at 105.432 kb on - strand, within PGA1_c01120 at 105.435 kb on + strand, within PGA1_c01120 at 105.440 kb on + strand, within PGA1_c01120 at 105.473 kb on - strand, within PGA1_c01120 at 105.510 kb on + strand, within PGA1_c01120 at 105.538 kb on - strand at 105.617 kb on + strand at 105.651 kb on + strand at 105.707 kb on - strand at 105.737 kb on - strand at 105.737 kb on - strand at 105.737 kb on - strand at 105.745 kb on - strand at 105.782 kb on + strand, within PGA1_c01130 at 105.790 kb on - strand, within PGA1_c01130 at 105.790 kb on - strand, within PGA1_c01130 at 105.823 kb on - strand, within PGA1_c01130 at 105.833 kb on - strand, within PGA1_c01130 at 105.880 kb on + strand, within PGA1_c01130 at 105.910 kb on - strand, within PGA1_c01130 at 105.935 kb on + strand, within PGA1_c01130 at 106.001 kb on + strand, within PGA1_c01130 at 106.001 kb on + strand, within PGA1_c01130 at 106.009 kb on - strand, within PGA1_c01130 at 106.069 kb on + strand, within PGA1_c01130 at 106.112 kb on + strand, within PGA1_c01130 at 106.135 kb on - strand, within PGA1_c01130 at 106.145 kb on + strand, within PGA1_c01130 at 106.153 kb on - strand, within PGA1_c01130 at 106.207 kb on - strand, within PGA1_c01130 at 106.247 kb on + strand, within PGA1_c01130 at 106.254 kb on - strand, within PGA1_c01130 at 106.255 kb on - strand, within PGA1_c01130 at 106.277 kb on + strand, within PGA1_c01130 at 106.311 kb on - strand, within PGA1_c01130 at 106.369 kb on + strand, within PGA1_c01130 at 106.380 kb on + strand, within PGA1_c01130 at 106.384 kb on + strand, within PGA1_c01130 at 106.463 kb on + strand, within PGA1_c01130 at 106.498 kb on + strand at 106.507 kb on - strand at 106.537 kb on - strand at 106.548 kb on + strand at 106.553 kb on + strand at 106.553 kb on + strand at 106.556 kb on - strand at 106.601 kb on + strand at 106.601 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0032 mg/ml remove 103,843 + PGA1_c01110 0.51 +1.5 103,928 - PGA1_c01110 0.56 -3.2 103,928 - PGA1_c01110 0.56 -1.2 103,930 + PGA1_c01110 0.56 -1.0 103,946 + PGA1_c01110 0.57 +1.8 103,954 - PGA1_c01110 0.58 +4.4 103,954 - PGA1_c01110 0.58 +2.1 103,954 - PGA1_c01110 0.58 +3.2 103,961 - PGA1_c01110 0.58 -3.0 103,961 - PGA1_c01110 0.58 +2.9 103,961 - PGA1_c01110 0.58 -2.6 103,961 - PGA1_c01110 0.58 +1.7 103,963 + PGA1_c01110 0.58 -1.8 103,963 + PGA1_c01110 0.58 +0.6 103,963 + PGA1_c01110 0.58 -0.8 103,963 + PGA1_c01110 0.58 +1.3 104,052 + PGA1_c01110 0.63 +2.9 104,053 - PGA1_c01110 0.63 -2.1 104,080 + PGA1_c01110 0.65 -1.6 104,151 - PGA1_c01110 0.69 -2.3 104,151 - PGA1_c01110 0.69 +3.2 104,174 - PGA1_c01110 0.71 -2.0 104,174 - PGA1_c01110 0.71 +2.9 104,174 - PGA1_c01110 0.71 +0.5 104,195 - PGA1_c01110 0.72 -0.9 104,223 - PGA1_c01110 0.74 +2.8 104,240 + PGA1_c01110 0.75 -3.1 104,245 + PGA1_c01110 0.75 +2.2 104,314 - PGA1_c01110 0.79 -1.8 104,334 + PGA1_c01110 0.80 -0.8 104,342 - PGA1_c01110 0.81 +3.2 104,409 + PGA1_c01110 0.85 +1.2 104,409 + PGA1_c01110 0.85 -3.2 104,417 - PGA1_c01110 0.85 +1.6 104,417 - PGA1_c01110 0.85 -0.9 104,417 - PGA1_c01110 0.85 +0.1 104,417 - PGA1_c01110 0.85 -1.9 104,417 - PGA1_c01110 0.85 +0.5 104,419 + PGA1_c01110 0.85 -1.6 104,419 - PGA1_c01110 0.85 -2.0 104,454 - PGA1_c01110 0.87 -0.9 104,454 - PGA1_c01110 0.87 +1.5 104,456 - PGA1_c01110 0.87 -1.9 104,474 - PGA1_c01110 0.88 +1.5 104,497 - PGA1_c01110 0.90 -2.4 104,527 + -0.2 104,647 + -0.4 104,647 + -2.0 104,671 + +1.1 104,679 + -1.1 104,806 + -2.3 104,806 + -0.8 104,811 + +2.5 104,814 - -2.7 104,849 + -0.8 104,854 + -1.2 104,854 + +3.6 104,854 + +3.2 104,856 + -2.7 104,862 - +2.4 104,862 - +1.3 104,862 - -2.6 104,862 - +2.3 104,864 + -1.9 104,864 + -2.4 104,864 + -1.9 104,864 - +1.8 104,872 - -2.2 104,883 - +1.8 104,883 - +1.6 104,952 - PGA1_c01120 0.17 -1.7 104,957 + PGA1_c01120 0.18 +2.8 104,962 + PGA1_c01120 0.19 -2.4 104,965 - PGA1_c01120 0.19 -2.1 105,008 - PGA1_c01120 0.24 +2.0 105,036 - PGA1_c01120 0.28 -1.0 105,117 + PGA1_c01120 0.38 +0.7 105,117 + PGA1_c01120 0.38 +2.0 105,122 + PGA1_c01120 0.39 +1.6 105,130 - PGA1_c01120 0.40 -0.8 105,160 - PGA1_c01120 0.44 -0.4 105,297 + PGA1_c01120 0.61 -0.7 105,340 - PGA1_c01120 0.67 +1.7 105,357 + PGA1_c01120 0.69 -2.3 105,405 - PGA1_c01120 0.75 +0.1 105,414 + PGA1_c01120 0.76 -0.5 105,424 + PGA1_c01120 0.77 -1.3 105,424 + PGA1_c01120 0.77 -0.4 105,424 + PGA1_c01120 0.77 +2.4 105,427 - PGA1_c01120 0.78 +4.5 105,432 - PGA1_c01120 0.78 +1.6 105,435 + PGA1_c01120 0.79 +1.1 105,440 + PGA1_c01120 0.79 -2.4 105,473 - PGA1_c01120 0.83 -2.8 105,510 + PGA1_c01120 0.88 -0.8 105,538 - +2.7 105,617 + +0.7 105,651 + +0.7 105,707 - +1.9 105,737 - +1.0 105,737 - +0.9 105,737 - +1.5 105,745 - -0.6 105,782 + PGA1_c01130 0.13 -0.4 105,790 - PGA1_c01130 0.14 -1.3 105,790 - PGA1_c01130 0.14 +0.9 105,823 - PGA1_c01130 0.18 +0.3 105,833 - PGA1_c01130 0.19 +1.1 105,880 + PGA1_c01130 0.24 -3.0 105,910 - PGA1_c01130 0.28 +0.9 105,935 + PGA1_c01130 0.30 -0.4 106,001 + PGA1_c01130 0.38 -0.8 106,001 + PGA1_c01130 0.38 -2.7 106,009 - PGA1_c01130 0.39 +3.8 106,069 + PGA1_c01130 0.45 -1.2 106,112 + PGA1_c01130 0.50 +1.5 106,135 - PGA1_c01130 0.53 -1.3 106,145 + PGA1_c01130 0.54 +1.1 106,153 - PGA1_c01130 0.55 -0.3 106,207 - PGA1_c01130 0.61 -1.6 106,247 + PGA1_c01130 0.65 -2.1 106,254 - PGA1_c01130 0.66 +0.3 106,255 - PGA1_c01130 0.66 -2.1 106,277 + PGA1_c01130 0.68 -1.8 106,311 - PGA1_c01130 0.72 +2.8 106,369 + PGA1_c01130 0.79 +2.8 106,380 + PGA1_c01130 0.80 -1.4 106,384 + PGA1_c01130 0.80 -1.8 106,463 + PGA1_c01130 0.89 +1.5 106,498 + -3.0 106,507 - +0.8 106,537 - +2.3 106,548 + -0.7 106,553 + +2.7 106,553 + +0.4 106,556 - +2.7 106,601 + -0.4 106,601 + -1.2
Or see this region's nucleotide sequence