Experiment: Varel_Bryant medium with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT_3258 and BT3259 are separated by 107 nucleotides BT3259 and BT3260 are separated by 332 nucleotides BT3260 and BT3261 are separated by 88 nucleotides
BT_3258: BT_3258 - two-component system response regulator (NCBI), at 4,160,463 to 4,161,242
_3258
BT3259: BT3259 - hypothetical protein (NCBI ptt file), at 4,161,350 to 4,161,745
BT3259
BT3260: BT3260 - DNA polymerase I (NCBI ptt file), at 4,162,078 to 4,164,927
BT3260
BT3261: BT3261 - octaprenyl-diphosphate synthase (NCBI ptt file), at 4,165,016 to 4,165,990
BT3261
Position (kb)
4162
4163
4164
4165 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4161.086 kb on + strand, within BT_3258 at 4161.176 kb on + strand at 4161.254 kb on + strand at 4161.254 kb on + strand at 4161.259 kb on + strand at 4161.259 kb on + strand at 4161.308 kb on + strand at 4161.309 kb on - strand at 4161.309 kb on - strand at 4161.329 kb on + strand at 4161.329 kb on + strand at 4161.352 kb on - strand at 4161.359 kb on - strand at 4161.361 kb on + strand at 4161.400 kb on - strand, within BT3259 at 4161.425 kb on - strand, within BT3259 at 4161.456 kb on + strand, within BT3259 at 4161.460 kb on + strand, within BT3259 at 4161.461 kb on - strand, within BT3259 at 4161.461 kb on - strand, within BT3259 at 4161.550 kb on - strand, within BT3259 at 4161.553 kb on + strand, within BT3259 at 4161.555 kb on + strand, within BT3259 at 4161.561 kb on + strand, within BT3259 at 4161.568 kb on + strand, within BT3259 at 4161.584 kb on - strand, within BT3259 at 4161.584 kb on - strand, within BT3259 at 4161.584 kb on - strand, within BT3259 at 4161.614 kb on + strand, within BT3259 at 4161.643 kb on + strand, within BT3259 at 4161.643 kb on + strand, within BT3259 at 4161.663 kb on + strand, within BT3259 at 4161.664 kb on - strand, within BT3259 at 4161.704 kb on + strand, within BT3259 at 4161.711 kb on - strand at 4161.759 kb on + strand at 4161.795 kb on + strand at 4161.795 kb on + strand at 4161.796 kb on - strand at 4161.831 kb on - strand at 4161.847 kb on - strand at 4162.044 kb on + strand at 4162.067 kb on + strand at 4162.075 kb on - strand at 4162.080 kb on - strand at 4162.178 kb on - strand at 4162.178 kb on - strand at 4162.226 kb on - strand at 4162.250 kb on - strand at 4162.997 kb on - strand, within BT3260 at 4163.251 kb on - strand, within BT3260 at 4163.254 kb on - strand, within BT3260 at 4163.381 kb on + strand, within BT3260 at 4163.951 kb on - strand, within BT3260 at 4163.951 kb on - strand, within BT3260 at 4164.971 kb on + strand at 4164.983 kb on + strand at 4164.983 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Dimethyl Sulfoxide 2 vol% remove 4,161,086 + BT_3258 0.80 -0.9 4,161,176 + -0.8 4,161,254 + -0.6 4,161,254 + -2.0 4,161,259 + -1.3 4,161,259 + -0.8 4,161,308 + -0.3 4,161,309 - -0.8 4,161,309 - +1.0 4,161,329 + -0.8 4,161,329 + -2.1 4,161,352 - -0.5 4,161,359 - -0.7 4,161,361 + -0.9 4,161,400 - BT3259 0.13 +0.2 4,161,425 - BT3259 0.19 -0.6 4,161,456 + BT3259 0.27 -1.2 4,161,460 + BT3259 0.28 +0.3 4,161,461 - BT3259 0.28 -1.1 4,161,461 - BT3259 0.28 -1.3 4,161,550 - BT3259 0.51 +0.0 4,161,553 + BT3259 0.51 -0.7 4,161,555 + BT3259 0.52 +0.0 4,161,561 + BT3259 0.53 -0.1 4,161,568 + BT3259 0.55 +0.4 4,161,584 - BT3259 0.59 -1.3 4,161,584 - BT3259 0.59 -1.0 4,161,584 - BT3259 0.59 -1.3 4,161,614 + BT3259 0.67 -0.6 4,161,643 + BT3259 0.74 -0.7 4,161,643 + BT3259 0.74 -1.8 4,161,663 + BT3259 0.79 +1.0 4,161,664 - BT3259 0.79 -0.2 4,161,704 + BT3259 0.89 +0.0 4,161,711 - -0.1 4,161,759 + -0.3 4,161,795 + -1.1 4,161,795 + +1.6 4,161,796 - -1.6 4,161,831 - -1.2 4,161,847 - +1.0 4,162,044 + -0.5 4,162,067 + +0.0 4,162,075 - +1.0 4,162,080 - -2.4 4,162,178 - -1.2 4,162,178 - -2.5 4,162,226 - -0.6 4,162,250 - -0.8 4,162,997 - BT3260 0.32 -1.6 4,163,251 - BT3260 0.41 -2.3 4,163,254 - BT3260 0.41 -2.4 4,163,381 + BT3260 0.46 -3.3 4,163,951 - BT3260 0.66 -2.8 4,163,951 - BT3260 0.66 -3.0 4,164,971 + -1.6 4,164,983 + +1.6 4,164,983 + -1.0
Or see this region's nucleotide sequence