Experiment: Varel_Bryant medium with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1966 and BT1967 are separated by 18 nucleotides BT1967 and BT1968 are separated by 154 nucleotides
BT1966: BT1966 - multidrug resistance protein, AcrB/AcrD family (NCBI ptt file), at 2,466,692 to 2,469,889
BT1966
BT1967: BT1967 - multidrug efflux protein (NCBI ptt file), at 2,469,908 to 2,471,050
BT1967
BT1968: BT1968 - transcriptional regulator (NCBI ptt file), at 2,471,205 to 2,472,053
BT1968
Position (kb)
2469
2470
2471
2472 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2468.969 kb on + strand, within BT1966 at 2469.066 kb on + strand, within BT1966 at 2469.067 kb on - strand, within BT1966 at 2469.067 kb on - strand, within BT1966 at 2469.067 kb on - strand, within BT1966 at 2469.072 kb on + strand, within BT1966 at 2469.072 kb on + strand, within BT1966 at 2469.072 kb on + strand, within BT1966 at 2469.072 kb on + strand, within BT1966 at 2469.077 kb on - strand, within BT1966 at 2469.187 kb on + strand, within BT1966 at 2469.188 kb on - strand, within BT1966 at 2469.200 kb on - strand, within BT1966 at 2469.200 kb on - strand, within BT1966 at 2469.300 kb on + strand, within BT1966 at 2469.338 kb on - strand, within BT1966 at 2469.441 kb on + strand, within BT1966 at 2469.446 kb on - strand, within BT1966 at 2469.522 kb on - strand, within BT1966 at 2469.628 kb on + strand at 2469.657 kb on + strand at 2469.658 kb on - strand at 2469.738 kb on + strand at 2469.742 kb on - strand at 2469.838 kb on + strand at 2469.857 kb on + strand at 2469.857 kb on + strand at 2469.872 kb on - strand at 2469.882 kb on - strand at 2469.909 kb on + strand at 2469.928 kb on + strand at 2469.929 kb on - strand at 2469.980 kb on + strand at 2469.981 kb on - strand at 2470.091 kb on + strand, within BT1967 at 2470.095 kb on - strand, within BT1967 at 2470.095 kb on - strand, within BT1967 at 2470.100 kb on - strand, within BT1967 at 2470.101 kb on + strand, within BT1967 at 2470.103 kb on + strand, within BT1967 at 2470.104 kb on - strand, within BT1967 at 2470.104 kb on - strand, within BT1967 at 2470.107 kb on + strand, within BT1967 at 2470.107 kb on + strand, within BT1967 at 2470.181 kb on + strand, within BT1967 at 2470.280 kb on - strand, within BT1967 at 2470.426 kb on - strand, within BT1967 at 2470.426 kb on - strand, within BT1967 at 2470.426 kb on - strand, within BT1967 at 2470.478 kb on + strand, within BT1967 at 2470.478 kb on + strand, within BT1967 at 2470.512 kb on - strand, within BT1967 at 2470.544 kb on - strand, within BT1967 at 2470.555 kb on + strand, within BT1967 at 2470.653 kb on - strand, within BT1967 at 2470.857 kb on + strand, within BT1967 at 2470.937 kb on - strand at 2471.013 kb on - strand at 2471.151 kb on - strand at 2471.175 kb on - strand at 2471.200 kb on + strand at 2471.200 kb on + strand at 2471.201 kb on - strand at 2471.204 kb on + strand at 2471.280 kb on - strand at 2471.320 kb on - strand, within BT1968 at 2471.396 kb on - strand, within BT1968 at 2471.474 kb on + strand, within BT1968 at 2471.523 kb on - strand, within BT1968 at 2471.542 kb on - strand, within BT1968 at 2471.584 kb on + strand, within BT1968 at 2471.584 kb on + strand, within BT1968 at 2471.604 kb on + strand, within BT1968 at 2471.604 kb on + strand, within BT1968 at 2471.681 kb on + strand, within BT1968 at 2471.682 kb on - strand, within BT1968 at 2471.695 kb on - strand, within BT1968 at 2471.762 kb on - strand, within BT1968 at 2471.795 kb on + strand, within BT1968 at 2471.914 kb on + strand, within BT1968 at 2471.949 kb on - strand, within BT1968 at 2471.949 kb on - strand, within BT1968 at 2472.014 kb on + strand at 2472.043 kb on - strand at 2472.043 kb on - strand at 2472.043 kb on - strand at 2472.048 kb on - strand at 2472.048 kb on - strand at 2472.049 kb on + strand at 2472.049 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Dimethyl Sulfoxide 2 vol% remove 2,468,969 + BT1966 0.71 -1.0 2,469,066 + BT1966 0.74 +1.0 2,469,067 - BT1966 0.74 -0.0 2,469,067 - BT1966 0.74 -0.2 2,469,067 - BT1966 0.74 -2.0 2,469,072 + BT1966 0.74 -0.0 2,469,072 + BT1966 0.74 -0.6 2,469,072 + BT1966 0.74 -0.7 2,469,072 + BT1966 0.74 +0.2 2,469,077 - BT1966 0.75 -1.9 2,469,187 + BT1966 0.78 -1.6 2,469,188 - BT1966 0.78 -0.2 2,469,200 - BT1966 0.78 -1.8 2,469,200 - BT1966 0.78 -0.0 2,469,300 + BT1966 0.82 -1.0 2,469,338 - BT1966 0.83 -0.9 2,469,441 + BT1966 0.86 -0.4 2,469,446 - BT1966 0.86 +1.0 2,469,522 - BT1966 0.88 -1.0 2,469,628 + -0.1 2,469,657 + +1.0 2,469,658 - -0.6 2,469,738 + +0.4 2,469,742 - -0.0 2,469,838 + -3.0 2,469,857 + -0.1 2,469,857 + +0.4 2,469,872 - -1.9 2,469,882 - -0.8 2,469,909 + -1.7 2,469,928 + -1.4 2,469,929 - -1.5 2,469,980 + -2.0 2,469,981 - +1.0 2,470,091 + BT1967 0.16 -1.0 2,470,095 - BT1967 0.16 -1.4 2,470,095 - BT1967 0.16 -0.0 2,470,100 - BT1967 0.17 -0.5 2,470,101 + BT1967 0.17 -1.3 2,470,103 + BT1967 0.17 -0.8 2,470,104 - BT1967 0.17 +0.4 2,470,104 - BT1967 0.17 -2.0 2,470,107 + BT1967 0.17 -1.2 2,470,107 + BT1967 0.17 -0.0 2,470,181 + BT1967 0.24 -0.7 2,470,280 - BT1967 0.33 +0.4 2,470,426 - BT1967 0.45 +0.2 2,470,426 - BT1967 0.45 -1.4 2,470,426 - BT1967 0.45 +1.0 2,470,478 + BT1967 0.50 -0.1 2,470,478 + BT1967 0.50 +0.4 2,470,512 - BT1967 0.53 +0.4 2,470,544 - BT1967 0.56 -1.0 2,470,555 + BT1967 0.57 -1.0 2,470,653 - BT1967 0.65 -0.7 2,470,857 + BT1967 0.83 -0.6 2,470,937 - +1.6 2,471,013 - -1.4 2,471,151 - -1.6 2,471,175 - -0.0 2,471,200 + -1.3 2,471,200 + +1.0 2,471,201 - -0.0 2,471,204 + -1.8 2,471,280 - -0.0 2,471,320 - BT1968 0.14 +2.0 2,471,396 - BT1968 0.22 -1.8 2,471,474 + BT1968 0.32 +1.0 2,471,523 - BT1968 0.37 -0.7 2,471,542 - BT1968 0.40 -0.6 2,471,584 + BT1968 0.45 -0.6 2,471,584 + BT1968 0.45 -0.7 2,471,604 + BT1968 0.47 -1.7 2,471,604 + BT1968 0.47 +1.0 2,471,681 + BT1968 0.56 +1.0 2,471,682 - BT1968 0.56 -1.5 2,471,695 - BT1968 0.58 -1.6 2,471,762 - BT1968 0.66 -0.4 2,471,795 + BT1968 0.69 +1.0 2,471,914 + BT1968 0.84 -1.3 2,471,949 - BT1968 0.88 -0.6 2,471,949 - BT1968 0.88 -0.6 2,472,014 + -0.0 2,472,043 - +1.0 2,472,043 - -0.6 2,472,043 - -0.8 2,472,048 - -0.8 2,472,048 - -1.0 2,472,049 + -0.6 2,472,049 + -1.7
Or see this region's nucleotide sequence