Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=2hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_03910 and IAI47_03915 are separated by 684 nucleotides IAI47_03915 and IAI47_03920 are separated by 12 nucleotides
IAI47_03910: IAI47_03910 - DNA mismatch repair endonuclease MutH, at 814,349 to 815,026
_03910
IAI47_03915: IAI47_03915 - RNA pyrophosphohydrolase, at 815,711 to 816,238
_03915
IAI47_03920: IAI47_03920 - phosphoenolpyruvate--protein phosphotransferase, at 816,251 to 818,497
_03920
Position (kb) 815
816
817 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 814.865 kb on + strand, within IAI47_03910 at 814.865 kb on + strand, within IAI47_03910 at 814.865 kb on + strand, within IAI47_03910 at 814.866 kb on - strand, within IAI47_03910 at 814.866 kb on - strand, within IAI47_03910 at 814.866 kb on - strand, within IAI47_03910 at 814.866 kb on - strand, within IAI47_03910 at 814.894 kb on + strand, within IAI47_03910 at 814.894 kb on + strand, within IAI47_03910 at 814.894 kb on + strand, within IAI47_03910 at 814.895 kb on - strand, within IAI47_03910 at 814.903 kb on + strand, within IAI47_03910 at 814.955 kb on + strand, within IAI47_03910 at 814.955 kb on + strand, within IAI47_03910 at 814.956 kb on - strand, within IAI47_03910 at 815.019 kb on + strand at 815.020 kb on - strand at 815.020 kb on - strand at 815.032 kb on + strand at 815.032 kb on + strand at 815.032 kb on + strand at 815.032 kb on + strand at 815.033 kb on - strand at 815.109 kb on + strand at 815.110 kb on - strand at 815.112 kb on + strand at 815.112 kb on + strand at 815.112 kb on + strand at 815.112 kb on + strand at 815.112 kb on + strand at 815.112 kb on + strand at 815.113 kb on - strand at 815.113 kb on - strand at 815.120 kb on + strand at 815.120 kb on + strand at 815.121 kb on - strand at 815.121 kb on - strand at 815.121 kb on - strand at 815.175 kb on + strand at 815.175 kb on + strand at 815.185 kb on + strand at 815.248 kb on - strand at 815.271 kb on + strand at 815.271 kb on + strand at 815.271 kb on + strand at 815.271 kb on + strand at 815.272 kb on - strand at 815.272 kb on - strand at 815.272 kb on - strand at 815.272 kb on - strand at 815.272 kb on - strand at 815.272 kb on - strand at 815.300 kb on + strand at 815.320 kb on - strand at 815.334 kb on - strand at 815.366 kb on + strand at 815.392 kb on + strand at 815.443 kb on + strand at 815.447 kb on + strand at 815.453 kb on - strand at 815.554 kb on + strand at 815.584 kb on - strand at 815.660 kb on + strand at 815.660 kb on + strand at 815.660 kb on + strand at 815.660 kb on + strand at 816.181 kb on + strand, within IAI47_03915 at 816.181 kb on + strand, within IAI47_03915 at 816.181 kb on + strand, within IAI47_03915 at 816.181 kb on + strand, within IAI47_03915 at 816.181 kb on + strand, within IAI47_03915 at 816.181 kb on + strand, within IAI47_03915 at 816.181 kb on + strand, within IAI47_03915 at 816.181 kb on + strand, within IAI47_03915 at 816.181 kb on + strand, within IAI47_03915 at 816.182 kb on + strand, within IAI47_03915 at 816.182 kb on - strand, within IAI47_03915 at 816.182 kb on - strand, within IAI47_03915 at 816.182 kb on - strand, within IAI47_03915 at 816.182 kb on - strand, within IAI47_03915 at 816.182 kb on - strand, within IAI47_03915 at 816.209 kb on - strand at 816.226 kb on + strand at 816.227 kb on - strand at 816.227 kb on - strand at 816.227 kb on - strand at 816.250 kb on + strand at 816.250 kb on + strand at 816.250 kb on + strand at 816.250 kb on + strand at 816.250 kb on + strand at 816.250 kb on + strand at 816.250 kb on + strand at 816.250 kb on + strand at 816.251 kb on - strand at 816.251 kb on - strand at 816.251 kb on - strand at 816.251 kb on - strand at 816.371 kb on + strand at 816.371 kb on + strand at 816.372 kb on - strand at 816.398 kb on + strand at 816.398 kb on + strand at 816.399 kb on - strand at 816.399 kb on - strand at 816.401 kb on + strand at 816.401 kb on + strand at 816.401 kb on + strand at 816.402 kb on - strand at 816.402 kb on - strand at 816.447 kb on + strand at 816.448 kb on - strand at 816.448 kb on - strand at 816.469 kb on + strand at 816.480 kb on + strand, within IAI47_03920 at 816.503 kb on - strand, within IAI47_03920 at 816.506 kb on - strand, within IAI47_03920 at 816.542 kb on + strand, within IAI47_03920 at 816.542 kb on + strand, within IAI47_03920 at 816.542 kb on + strand, within IAI47_03920 at 816.542 kb on + strand, within IAI47_03920 at 816.542 kb on + strand, within IAI47_03920 at 816.542 kb on + strand, within IAI47_03920 at 816.542 kb on + strand, within IAI47_03920 at 816.542 kb on + strand, within IAI47_03920 at 816.542 kb on + strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.543 kb on - strand, within IAI47_03920 at 816.545 kb on - strand, within IAI47_03920 at 816.545 kb on - strand, within IAI47_03920 at 816.595 kb on + strand, within IAI47_03920 at 816.595 kb on + strand, within IAI47_03920 at 816.595 kb on + strand, within IAI47_03920 at 816.595 kb on + strand, within IAI47_03920 at 816.596 kb on - strand, within IAI47_03920 at 816.691 kb on + strand, within IAI47_03920 at 816.983 kb on + strand, within IAI47_03920 at 816.983 kb on + strand, within IAI47_03920 at 816.983 kb on + strand, within IAI47_03920 at 816.983 kb on + strand, within IAI47_03920 at 816.983 kb on + strand, within IAI47_03920 at 816.984 kb on - strand, within IAI47_03920 at 816.984 kb on - strand, within IAI47_03920 at 816.984 kb on - strand, within IAI47_03920 at 817.118 kb on + strand, within IAI47_03920 at 817.118 kb on + strand, within IAI47_03920 at 817.118 kb on + strand, within IAI47_03920 at 817.118 kb on + strand, within IAI47_03920 at 817.118 kb on + strand, within IAI47_03920 at 817.118 kb on + strand, within IAI47_03920 at 817.118 kb on + strand, within IAI47_03920 at 817.119 kb on - strand, within IAI47_03920 at 817.119 kb on - strand, within IAI47_03920 at 817.119 kb on - strand, within IAI47_03920 at 817.119 kb on - strand, within IAI47_03920 at 817.119 kb on - strand, within IAI47_03920 at 817.119 kb on - strand, within IAI47_03920 at 817.119 kb on - strand, within IAI47_03920 at 817.119 kb on - strand, within IAI47_03920 at 817.150 kb on - strand, within IAI47_03920
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=2hrs; Outgrowth=LBkan50 remove 814,865 + IAI47_03910 0.76 +0.5 814,865 + IAI47_03910 0.76 -0.9 814,865 + IAI47_03910 0.76 -0.7 814,866 - IAI47_03910 0.76 +1.2 814,866 - IAI47_03910 0.76 -1.5 814,866 - IAI47_03910 0.76 -0.2 814,866 - IAI47_03910 0.76 +1.2 814,894 + IAI47_03910 0.80 -0.5 814,894 + IAI47_03910 0.80 -1.8 814,894 + IAI47_03910 0.80 +0.9 814,895 - IAI47_03910 0.81 +0.2 814,903 + IAI47_03910 0.82 -0.8 814,955 + IAI47_03910 0.89 +0.3 814,955 + IAI47_03910 0.89 -0.1 814,956 - IAI47_03910 0.90 +0.6 815,019 + +0.7 815,020 - +1.0 815,020 - -0.4 815,032 + -2.6 815,032 + -1.4 815,032 + -0.7 815,032 + +2.0 815,033 - +0.4 815,109 + -0.2 815,110 - -0.9 815,112 + -0.7 815,112 + +1.8 815,112 + -0.0 815,112 + -0.9 815,112 + +0.8 815,112 + +0.5 815,113 - -3.2 815,113 - +0.5 815,120 + +0.6 815,120 + +0.8 815,121 - -1.3 815,121 - -0.2 815,121 - +0.0 815,175 + +0.1 815,175 + -0.5 815,185 + -1.5 815,248 - +0.8 815,271 + +1.8 815,271 + -0.1 815,271 + -0.0 815,271 + -0.2 815,272 - +1.1 815,272 - +0.6 815,272 - +0.4 815,272 - +0.3 815,272 - +0.3 815,272 - +0.2 815,300 + +2.2 815,320 - -0.3 815,334 - -0.4 815,366 + +0.1 815,392 + -0.2 815,443 + +0.9 815,447 + +1.4 815,453 - -1.5 815,554 + +0.9 815,584 - -0.9 815,660 + +0.7 815,660 + -1.7 815,660 + -0.9 815,660 + -0.6 816,181 + IAI47_03915 0.89 +0.0 816,181 + IAI47_03915 0.89 +0.0 816,181 + IAI47_03915 0.89 +0.6 816,181 + IAI47_03915 0.89 +0.6 816,181 + IAI47_03915 0.89 +0.1 816,181 + IAI47_03915 0.89 +2.6 816,181 + IAI47_03915 0.89 +1.2 816,181 + IAI47_03915 0.89 +3.4 816,181 + IAI47_03915 0.89 +0.9 816,182 + IAI47_03915 0.89 +1.0 816,182 - IAI47_03915 0.89 -0.4 816,182 - IAI47_03915 0.89 -2.5 816,182 - IAI47_03915 0.89 +1.2 816,182 - IAI47_03915 0.89 -1.1 816,182 - IAI47_03915 0.89 +0.4 816,209 - -0.7 816,226 + +0.1 816,227 - -0.1 816,227 - -0.9 816,227 - -1.5 816,250 + +0.5 816,250 + +0.6 816,250 + +0.1 816,250 + +1.1 816,250 + +0.2 816,250 + +3.2 816,250 + +1.8 816,250 + +0.3 816,251 - +2.3 816,251 - -0.3 816,251 - -0.3 816,251 - +0.3 816,371 + -0.6 816,371 + -1.9 816,372 - -0.2 816,398 + +0.1 816,398 + +0.1 816,399 - +0.6 816,399 - +0.8 816,401 + +1.2 816,401 + +0.5 816,401 + -0.4 816,402 - -0.4 816,402 - +1.0 816,447 + -0.5 816,448 - -1.1 816,448 - -0.9 816,469 + -0.1 816,480 + IAI47_03920 0.10 +0.1 816,503 - IAI47_03920 0.11 -0.6 816,506 - IAI47_03920 0.11 -1.4 816,542 + IAI47_03920 0.13 -2.3 816,542 + IAI47_03920 0.13 +0.1 816,542 + IAI47_03920 0.13 +0.4 816,542 + IAI47_03920 0.13 -0.3 816,542 + IAI47_03920 0.13 +1.0 816,542 + IAI47_03920 0.13 +0.7 816,542 + IAI47_03920 0.13 +0.3 816,542 + IAI47_03920 0.13 +1.1 816,542 + IAI47_03920 0.13 +0.2 816,543 - IAI47_03920 0.13 -1.2 816,543 - IAI47_03920 0.13 +2.7 816,543 - IAI47_03920 0.13 +1.0 816,543 - IAI47_03920 0.13 +0.1 816,543 - IAI47_03920 0.13 +3.4 816,543 - IAI47_03920 0.13 -0.4 816,543 - IAI47_03920 0.13 +0.6 816,543 - IAI47_03920 0.13 +2.1 816,543 - IAI47_03920 0.13 +0.1 816,543 - IAI47_03920 0.13 -0.3 816,543 - IAI47_03920 0.13 +0.1 816,543 - IAI47_03920 0.13 +1.0 816,543 - IAI47_03920 0.13 +0.0 816,545 - IAI47_03920 0.13 -1.1 816,545 - IAI47_03920 0.13 -0.7 816,595 + IAI47_03920 0.15 +0.8 816,595 + IAI47_03920 0.15 +1.2 816,595 + IAI47_03920 0.15 -1.1 816,595 + IAI47_03920 0.15 +3.8 816,596 - IAI47_03920 0.15 +1.0 816,691 + IAI47_03920 0.20 +0.6 816,983 + IAI47_03920 0.33 -2.2 816,983 + IAI47_03920 0.33 -1.0 816,983 + IAI47_03920 0.33 -1.2 816,983 + IAI47_03920 0.33 -0.4 816,983 + IAI47_03920 0.33 +2.5 816,984 - IAI47_03920 0.33 +0.3 816,984 - IAI47_03920 0.33 -0.8 816,984 - IAI47_03920 0.33 -0.9 817,118 + IAI47_03920 0.39 -0.1 817,118 + IAI47_03920 0.39 -0.2 817,118 + IAI47_03920 0.39 -0.0 817,118 + IAI47_03920 0.39 +0.3 817,118 + IAI47_03920 0.39 +0.3 817,118 + IAI47_03920 0.39 -1.2 817,118 + IAI47_03920 0.39 +1.7 817,119 - IAI47_03920 0.39 +0.9 817,119 - IAI47_03920 0.39 +0.9 817,119 - IAI47_03920 0.39 +0.3 817,119 - IAI47_03920 0.39 +0.5 817,119 - IAI47_03920 0.39 +1.2 817,119 - IAI47_03920 0.39 -0.6 817,119 - IAI47_03920 0.39 +0.8 817,119 - IAI47_03920 0.39 +1.9 817,150 - IAI47_03920 0.40 +0.6
Or see this region's nucleotide sequence