Experiment: Varel_Bryant medium with Tetrazolium violet 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1575 and BT1576 are separated by 60 nucleotides BT1576 and BT1577 are separated by 34 nucleotides BT1577 and BT1578 are separated by 68 nucleotides
BT1575: BT1575 - putative dehydrogenase (NCBI ptt file), at 1,939,570 to 1,940,571
BT1575
BT1576: BT1576 - conserved hypothetical protein, with a conserved domain (NCBI ptt file), at 1,940,632 to 1,941,336
BT1576
BT1577: BT1577 - pyridoxamine 5'-phosphate oxidase (NCBI ptt file), at 1,941,371 to 1,942,012
BT1577
BT1578: BT1578 - putative metal dependent hydrolase (NCBI ptt file), at 1,942,081 to 1,942,812
BT1578
Position (kb)
1940
1941
1942 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1939.664 kb on - strand at 1939.815 kb on + strand, within BT1575 at 1939.816 kb on - strand, within BT1575 at 1939.900 kb on - strand, within BT1575 at 1939.900 kb on - strand, within BT1575 at 1940.357 kb on - strand, within BT1575 at 1940.439 kb on + strand, within BT1575 at 1940.440 kb on - strand, within BT1575 at 1940.496 kb on + strand at 1940.496 kb on + strand at 1940.552 kb on + strand at 1940.569 kb on + strand at 1940.569 kb on + strand at 1940.608 kb on - strand at 1940.649 kb on + strand at 1940.650 kb on - strand at 1940.695 kb on + strand at 1940.696 kb on - strand at 1940.696 kb on - strand at 1940.818 kb on - strand, within BT1576 at 1941.077 kb on + strand, within BT1576 at 1941.094 kb on - strand, within BT1576 at 1941.111 kb on - strand, within BT1576 at 1941.221 kb on + strand, within BT1576 at 1941.237 kb on - strand, within BT1576 at 1941.271 kb on - strand at 1941.309 kb on + strand at 1941.309 kb on + strand at 1941.309 kb on + strand at 1941.310 kb on - strand at 1941.310 kb on - strand at 1941.320 kb on + strand at 1941.320 kb on + strand at 1941.321 kb on - strand at 1941.379 kb on + strand at 1941.381 kb on + strand at 1941.404 kb on - strand at 1941.517 kb on - strand, within BT1577 at 1941.589 kb on + strand, within BT1577 at 1941.596 kb on + strand, within BT1577 at 1941.606 kb on - strand, within BT1577 at 1941.606 kb on - strand, within BT1577 at 1941.738 kb on - strand, within BT1577 at 1941.757 kb on + strand, within BT1577 at 1942.093 kb on + strand at 1942.100 kb on - strand at 1942.228 kb on + strand, within BT1578 at 1942.229 kb on - strand, within BT1578 at 1942.233 kb on - strand, within BT1578 at 1942.326 kb on + strand, within BT1578
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Tetrazolium violet 0.025 mM remove 1,939,664 - -1.5 1,939,815 + BT1575 0.24 -2.8 1,939,816 - BT1575 0.25 +1.0 1,939,900 - BT1575 0.33 -1.2 1,939,900 - BT1575 0.33 +1.0 1,940,357 - BT1575 0.79 +1.0 1,940,439 + BT1575 0.87 +0.0 1,940,440 - BT1575 0.87 -0.6 1,940,496 + -0.6 1,940,496 + -0.8 1,940,552 + +2.6 1,940,569 + -0.6 1,940,569 + -1.2 1,940,608 - -1.2 1,940,649 + -0.4 1,940,650 - -1.3 1,940,695 + -3.1 1,940,696 - -1.4 1,940,696 - +0.4 1,940,818 - BT1576 0.26 -2.7 1,941,077 + BT1576 0.63 +0.0 1,941,094 - BT1576 0.66 -2.0 1,941,111 - BT1576 0.68 -2.3 1,941,221 + BT1576 0.84 +0.0 1,941,237 - BT1576 0.86 -1.0 1,941,271 - -1.6 1,941,309 + +0.0 1,941,309 + -1.4 1,941,309 + -0.8 1,941,310 - -1.1 1,941,310 - -1.6 1,941,320 + +0.3 1,941,320 + +0.7 1,941,321 - -0.6 1,941,379 + -1.4 1,941,381 + -1.1 1,941,404 - -1.3 1,941,517 - BT1577 0.23 -1.1 1,941,589 + BT1577 0.34 +1.0 1,941,596 + BT1577 0.35 +0.0 1,941,606 - BT1577 0.37 +1.0 1,941,606 - BT1577 0.37 -0.4 1,941,738 - BT1577 0.57 -1.1 1,941,757 + BT1577 0.60 -0.2 1,942,093 + +0.4 1,942,100 - +1.0 1,942,228 + BT1578 0.20 +0.0 1,942,229 - BT1578 0.20 -1.0 1,942,233 - BT1578 0.21 -0.9 1,942,326 + BT1578 0.33 -2.0
Or see this region's nucleotide sequence