Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=2hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20545 and IAI47_20550 are separated by 230 nucleotides IAI47_20550 and IAI47_20555 overlap by 13 nucleotides IAI47_20555 and IAI47_20560 overlap by 8 nucleotides IAI47_20560 and IAI47_20565 are separated by 19 nucleotides
IAI47_20545: IAI47_20545 - NAD(P)H-dependent oxidoreductase, at 359,077 to 359,679
_20545
IAI47_20550: IAI47_20550 - ABC transporter permease, at 359,910 to 360,578
_20550
IAI47_20555: IAI47_20555 - ATP-binding cassette domain-containing protein, at 360,566 to 361,354
_20555
IAI47_20560: IAI47_20560 - MetQ/NlpA family ABC transporter substrate-binding protein, at 361,347 to 362,150
_20560
IAI47_20565: IAI47_20565 - isopenicillin N synthase family oxygenase, at 362,170 to 363,189
_20565
Position (kb) 360
361
362 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 359.598 kb on + strand, within IAI47_20545 at 359.599 kb on - strand, within IAI47_20545 at 359.665 kb on + strand at 359.665 kb on + strand at 359.665 kb on + strand at 359.666 kb on - strand at 359.666 kb on - strand at 359.666 kb on - strand at 359.666 kb on - strand at 359.666 kb on - strand at 359.668 kb on + strand at 359.668 kb on + strand at 359.668 kb on + strand at 359.669 kb on - strand at 359.669 kb on - strand at 359.669 kb on - strand at 359.669 kb on - strand at 359.669 kb on - strand at 359.669 kb on - strand at 359.669 kb on - strand at 359.691 kb on + strand at 359.691 kb on + strand at 359.691 kb on + strand at 359.692 kb on - strand at 359.692 kb on - strand at 359.729 kb on - strand at 359.734 kb on + strand at 359.756 kb on - strand at 359.756 kb on - strand at 359.756 kb on - strand at 359.840 kb on - strand at 359.902 kb on + strand at 359.904 kb on + strand at 359.905 kb on - strand at 359.905 kb on - strand at 359.911 kb on + strand at 359.911 kb on + strand at 359.912 kb on - strand at 359.920 kb on + strand at 359.968 kb on - strand at 360.026 kb on - strand, within IAI47_20550 at 360.171 kb on - strand, within IAI47_20550 at 360.195 kb on + strand, within IAI47_20550 at 360.195 kb on + strand, within IAI47_20550 at 360.196 kb on - strand, within IAI47_20550 at 360.196 kb on - strand, within IAI47_20550 at 360.196 kb on - strand, within IAI47_20550 at 360.196 kb on - strand, within IAI47_20550 at 360.196 kb on - strand, within IAI47_20550 at 360.248 kb on + strand, within IAI47_20550 at 360.248 kb on + strand, within IAI47_20550 at 360.333 kb on - strand, within IAI47_20550 at 360.384 kb on - strand, within IAI47_20550 at 360.395 kb on + strand, within IAI47_20550 at 360.395 kb on + strand, within IAI47_20550 at 360.396 kb on - strand, within IAI47_20550 at 360.514 kb on + strand at 360.514 kb on + strand at 360.514 kb on + strand at 360.514 kb on + strand at 360.515 kb on - strand at 360.515 kb on - strand at 360.515 kb on - strand at 360.515 kb on - strand at 360.515 kb on - strand at 360.515 kb on - strand at 360.515 kb on - strand at 360.515 kb on - strand at 360.563 kb on + strand at 360.563 kb on + strand at 360.564 kb on - strand at 360.564 kb on - strand at 360.564 kb on - strand at 360.564 kb on - strand at 360.572 kb on + strand at 360.572 kb on + strand at 360.572 kb on + strand at 360.573 kb on - strand at 360.573 kb on - strand at 360.573 kb on - strand at 360.573 kb on - strand at 360.585 kb on - strand at 360.600 kb on + strand at 360.601 kb on - strand at 360.835 kb on + strand, within IAI47_20555 at 360.835 kb on + strand, within IAI47_20555 at 360.835 kb on + strand, within IAI47_20555 at 360.836 kb on - strand, within IAI47_20555 at 360.836 kb on - strand, within IAI47_20555 at 360.836 kb on - strand, within IAI47_20555 at 360.836 kb on - strand, within IAI47_20555 at 360.836 kb on - strand, within IAI47_20555 at 360.836 kb on - strand, within IAI47_20555 at 361.064 kb on + strand, within IAI47_20555 at 361.065 kb on - strand, within IAI47_20555 at 361.065 kb on - strand, within IAI47_20555 at 361.065 kb on - strand, within IAI47_20555 at 361.217 kb on + strand, within IAI47_20555 at 361.217 kb on + strand, within IAI47_20555 at 361.218 kb on - strand, within IAI47_20555 at 361.323 kb on + strand at 361.324 kb on - strand at 361.324 kb on - strand at 361.324 kb on - strand at 361.331 kb on - strand at 361.348 kb on + strand at 361.403 kb on + strand at 361.403 kb on + strand at 361.446 kb on + strand, within IAI47_20560 at 361.464 kb on - strand, within IAI47_20560 at 361.505 kb on + strand, within IAI47_20560 at 361.567 kb on + strand, within IAI47_20560 at 361.567 kb on + strand, within IAI47_20560 at 361.568 kb on - strand, within IAI47_20560 at 361.568 kb on - strand, within IAI47_20560 at 361.568 kb on - strand, within IAI47_20560 at 361.568 kb on - strand, within IAI47_20560 at 361.568 kb on - strand, within IAI47_20560 at 361.578 kb on - strand, within IAI47_20560 at 361.646 kb on + strand, within IAI47_20560 at 361.647 kb on - strand, within IAI47_20560 at 361.667 kb on + strand, within IAI47_20560 at 361.667 kb on + strand, within IAI47_20560 at 361.667 kb on + strand, within IAI47_20560 at 361.668 kb on - strand, within IAI47_20560 at 361.668 kb on - strand, within IAI47_20560 at 361.668 kb on - strand, within IAI47_20560 at 361.668 kb on - strand, within IAI47_20560 at 361.713 kb on + strand, within IAI47_20560 at 361.714 kb on - strand, within IAI47_20560 at 361.714 kb on - strand, within IAI47_20560 at 361.835 kb on - strand, within IAI47_20560 at 361.853 kb on + strand, within IAI47_20560 at 361.853 kb on + strand, within IAI47_20560 at 361.853 kb on + strand, within IAI47_20560 at 361.854 kb on - strand, within IAI47_20560 at 361.883 kb on + strand, within IAI47_20560 at 361.913 kb on - strand, within IAI47_20560 at 361.930 kb on + strand, within IAI47_20560 at 361.930 kb on + strand, within IAI47_20560 at 361.930 kb on + strand, within IAI47_20560 at 361.930 kb on + strand, within IAI47_20560 at 361.931 kb on - strand, within IAI47_20560 at 361.931 kb on - strand, within IAI47_20560 at 362.012 kb on + strand, within IAI47_20560 at 362.013 kb on - strand, within IAI47_20560 at 362.013 kb on - strand, within IAI47_20560 at 362.032 kb on + strand, within IAI47_20560 at 362.033 kb on - strand, within IAI47_20560 at 362.033 kb on - strand, within IAI47_20560 at 362.033 kb on - strand, within IAI47_20560 at 362.033 kb on - strand, within IAI47_20560 at 362.091 kb on + strand at 362.092 kb on - strand at 362.144 kb on + strand at 362.145 kb on - strand at 362.155 kb on + strand at 362.155 kb on + strand at 362.155 kb on + strand at 362.155 kb on + strand at 362.155 kb on + strand at 362.156 kb on - strand at 362.156 kb on - strand at 362.162 kb on + strand at 362.162 kb on + strand at 362.163 kb on - strand at 362.181 kb on + strand at 362.181 kb on + strand at 362.181 kb on + strand at 362.181 kb on + strand at 362.181 kb on + strand at 362.182 kb on - strand at 362.182 kb on - strand at 362.192 kb on + strand at 362.327 kb on + strand, within IAI47_20565 at 362.328 kb on - strand, within IAI47_20565 at 362.328 kb on - strand, within IAI47_20565 at 362.328 kb on - strand, within IAI47_20565 at 362.328 kb on - strand, within IAI47_20565 at 362.328 kb on - strand, within IAI47_20565
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=2hrs; Outgrowth=LBkan50 remove 359,598 + IAI47_20545 0.86 -1.0 359,599 - IAI47_20545 0.87 +0.6 359,665 + -0.1 359,665 + -0.4 359,665 + +0.5 359,666 - -0.4 359,666 - -1.6 359,666 - -0.7 359,666 - +0.4 359,666 - -0.5 359,668 + -1.5 359,668 + +0.5 359,668 + -1.0 359,669 - -0.5 359,669 - -0.3 359,669 - +0.4 359,669 - -0.5 359,669 - +0.9 359,669 - +0.4 359,669 - -2.0 359,691 + -1.3 359,691 + +0.8 359,691 + -0.6 359,692 - +1.9 359,692 - +0.9 359,729 - +0.1 359,734 + -1.1 359,756 - +0.9 359,756 - +1.4 359,756 - +0.0 359,840 - -0.2 359,902 + -0.4 359,904 + -0.3 359,905 - -0.5 359,905 - +1.1 359,911 + -0.2 359,911 + -0.6 359,912 - +0.9 359,920 + -0.9 359,968 - +0.7 360,026 - IAI47_20550 0.17 +0.9 360,171 - IAI47_20550 0.39 +0.8 360,195 + IAI47_20550 0.43 -0.7 360,195 + IAI47_20550 0.43 +0.8 360,196 - IAI47_20550 0.43 +1.5 360,196 - IAI47_20550 0.43 +0.6 360,196 - IAI47_20550 0.43 -0.2 360,196 - IAI47_20550 0.43 +0.4 360,196 - IAI47_20550 0.43 +1.0 360,248 + IAI47_20550 0.51 -1.5 360,248 + IAI47_20550 0.51 -0.1 360,333 - IAI47_20550 0.63 +1.4 360,384 - IAI47_20550 0.71 +0.8 360,395 + IAI47_20550 0.72 -0.7 360,395 + IAI47_20550 0.72 -0.1 360,396 - IAI47_20550 0.73 -0.3 360,514 + +0.3 360,514 + +0.5 360,514 + +1.0 360,514 + -0.4 360,515 - -0.2 360,515 - +2.4 360,515 - +0.9 360,515 - +0.6 360,515 - -1.0 360,515 - +0.0 360,515 - +1.2 360,515 - -1.5 360,563 + +1.5 360,563 + +1.7 360,564 - +0.3 360,564 - +1.2 360,564 - +2.6 360,564 - +0.4 360,572 + +0.8 360,572 + -2.1 360,572 + +0.0 360,573 - -0.0 360,573 - -0.8 360,573 - -0.2 360,573 - -0.3 360,585 - +0.3 360,600 + -0.2 360,601 - +0.8 360,835 + IAI47_20555 0.34 -1.1 360,835 + IAI47_20555 0.34 -0.6 360,835 + IAI47_20555 0.34 -0.1 360,836 - IAI47_20555 0.34 +0.1 360,836 - IAI47_20555 0.34 -0.7 360,836 - IAI47_20555 0.34 -1.3 360,836 - IAI47_20555 0.34 -0.5 360,836 - IAI47_20555 0.34 -1.0 360,836 - IAI47_20555 0.34 +0.9 361,064 + IAI47_20555 0.63 +0.9 361,065 - IAI47_20555 0.63 -2.2 361,065 - IAI47_20555 0.63 -0.6 361,065 - IAI47_20555 0.63 -1.7 361,217 + IAI47_20555 0.83 -1.1 361,217 + IAI47_20555 0.83 +1.4 361,218 - IAI47_20555 0.83 -0.8 361,323 + -0.6 361,324 - +1.5 361,324 - +0.3 361,324 - +3.7 361,331 - +0.9 361,348 + +0.2 361,403 + -0.5 361,403 + +0.2 361,446 + IAI47_20560 0.12 -0.8 361,464 - IAI47_20560 0.15 -1.4 361,505 + IAI47_20560 0.20 -0.1 361,567 + IAI47_20560 0.27 -2.8 361,567 + IAI47_20560 0.27 -1.8 361,568 - IAI47_20560 0.27 +1.3 361,568 - IAI47_20560 0.27 -0.7 361,568 - IAI47_20560 0.27 -1.0 361,568 - IAI47_20560 0.27 +0.4 361,568 - IAI47_20560 0.27 -0.5 361,578 - IAI47_20560 0.29 +2.6 361,646 + IAI47_20560 0.37 +2.2 361,647 - IAI47_20560 0.37 +0.2 361,667 + IAI47_20560 0.40 -1.0 361,667 + IAI47_20560 0.40 +0.1 361,667 + IAI47_20560 0.40 -0.5 361,668 - IAI47_20560 0.40 -0.9 361,668 - IAI47_20560 0.40 +0.9 361,668 - IAI47_20560 0.40 +0.9 361,668 - IAI47_20560 0.40 +0.9 361,713 + IAI47_20560 0.46 -0.4 361,714 - IAI47_20560 0.46 +0.6 361,714 - IAI47_20560 0.46 +0.9 361,835 - IAI47_20560 0.61 +0.7 361,853 + IAI47_20560 0.63 +0.1 361,853 + IAI47_20560 0.63 +0.4 361,853 + IAI47_20560 0.63 +0.1 361,854 - IAI47_20560 0.63 +0.0 361,883 + IAI47_20560 0.67 -0.5 361,913 - IAI47_20560 0.70 +0.3 361,930 + IAI47_20560 0.73 +0.7 361,930 + IAI47_20560 0.73 +0.9 361,930 + IAI47_20560 0.73 +1.5 361,930 + IAI47_20560 0.73 -0.2 361,931 - IAI47_20560 0.73 +1.1 361,931 - IAI47_20560 0.73 +0.0 362,012 + IAI47_20560 0.83 +0.7 362,013 - IAI47_20560 0.83 +0.9 362,013 - IAI47_20560 0.83 +2.0 362,032 + IAI47_20560 0.85 +1.3 362,033 - IAI47_20560 0.85 +0.0 362,033 - IAI47_20560 0.85 -1.0 362,033 - IAI47_20560 0.85 -1.5 362,033 - IAI47_20560 0.85 +0.0 362,091 + -0.1 362,092 - -1.1 362,144 + +0.3 362,145 - -0.7 362,155 + +1.4 362,155 + +0.4 362,155 + +1.3 362,155 + +1.9 362,155 + +0.6 362,156 - +0.5 362,156 - -0.0 362,162 + -0.7 362,162 + -2.3 362,163 - -1.3 362,181 + -0.2 362,181 + +0.1 362,181 + +0.1 362,181 + -0.1 362,181 + +1.9 362,182 - +2.4 362,182 - +0.4 362,192 + +1.8 362,327 + IAI47_20565 0.15 -0.8 362,328 - IAI47_20565 0.15 +1.3 362,328 - IAI47_20565 0.15 +1.5 362,328 - IAI47_20565 0.15 +1.6 362,328 - IAI47_20565 0.15 +0.6 362,328 - IAI47_20565 0.15 -1.3
Or see this region's nucleotide sequence