Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=1hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20570 and IAI47_20575 are separated by 116 nucleotides IAI47_20575 and IAI47_20580 are separated by 254 nucleotides IAI47_20580 and IAI47_20585 are separated by 14 nucleotides
IAI47_20570: IAI47_20570 - glycine betaine/L-proline transporter ProP, at 363,667 to 365,133
_20570
IAI47_20575: IAI47_20575 - YbhB/YbcL family Raf kinase inhibitor-like protein, at 365,250 to 365,798
_20575
IAI47_20580: IAI47_20580 - LysR family transcriptional regulator, at 366,053 to 366,931
_20580
IAI47_20585: IAI47_20585 - dihydroxyacetone kinase subunit DhaK, at 366,946 to 368,583
_20585
Position (kb) 366
367 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 365.062 kb on - strand at 365.062 kb on - strand at 365.062 kb on - strand at 365.062 kb on - strand at 365.082 kb on - strand at 365.106 kb on + strand at 365.107 kb on - strand at 365.131 kb on + strand at 365.131 kb on + strand at 365.131 kb on + strand at 365.131 kb on + strand at 365.131 kb on + strand at 365.132 kb on - strand at 365.132 kb on - strand at 365.132 kb on - strand at 365.132 kb on - strand at 365.197 kb on - strand at 365.197 kb on - strand at 365.197 kb on - strand at 365.235 kb on + strand at 365.236 kb on - strand at 365.251 kb on + strand at 365.259 kb on - strand at 365.259 kb on - strand at 365.259 kb on - strand at 365.358 kb on + strand, within IAI47_20575 at 365.358 kb on + strand, within IAI47_20575 at 365.384 kb on + strand, within IAI47_20575 at 365.384 kb on + strand, within IAI47_20575 at 365.384 kb on + strand, within IAI47_20575 at 365.384 kb on + strand, within IAI47_20575 at 365.384 kb on + strand, within IAI47_20575 at 365.384 kb on + strand, within IAI47_20575 at 365.384 kb on + strand, within IAI47_20575 at 365.384 kb on + strand, within IAI47_20575 at 365.384 kb on + strand, within IAI47_20575 at 365.385 kb on - strand, within IAI47_20575 at 365.385 kb on - strand, within IAI47_20575 at 365.385 kb on - strand, within IAI47_20575 at 365.386 kb on + strand, within IAI47_20575 at 365.386 kb on + strand, within IAI47_20575 at 365.386 kb on + strand, within IAI47_20575 at 365.386 kb on + strand, within IAI47_20575 at 365.387 kb on - strand, within IAI47_20575 at 365.387 kb on - strand, within IAI47_20575 at 365.387 kb on - strand, within IAI47_20575 at 365.428 kb on + strand, within IAI47_20575 at 365.428 kb on + strand, within IAI47_20575 at 365.429 kb on - strand, within IAI47_20575 at 365.429 kb on - strand, within IAI47_20575 at 365.463 kb on - strand, within IAI47_20575 at 365.463 kb on - strand, within IAI47_20575 at 365.464 kb on + strand, within IAI47_20575 at 365.464 kb on + strand, within IAI47_20575 at 365.464 kb on + strand, within IAI47_20575 at 365.464 kb on + strand, within IAI47_20575 at 365.465 kb on - strand, within IAI47_20575 at 365.465 kb on - strand, within IAI47_20575 at 365.465 kb on - strand, within IAI47_20575 at 365.465 kb on - strand, within IAI47_20575 at 365.465 kb on - strand, within IAI47_20575 at 365.522 kb on + strand, within IAI47_20575 at 365.522 kb on + strand, within IAI47_20575 at 365.522 kb on + strand, within IAI47_20575 at 365.522 kb on + strand, within IAI47_20575 at 365.523 kb on - strand, within IAI47_20575 at 365.523 kb on - strand, within IAI47_20575 at 365.524 kb on + strand, within IAI47_20575 at 365.524 kb on + strand, within IAI47_20575 at 365.524 kb on + strand, within IAI47_20575 at 365.525 kb on - strand, within IAI47_20575 at 365.525 kb on - strand, within IAI47_20575 at 365.661 kb on - strand, within IAI47_20575 at 365.661 kb on - strand, within IAI47_20575 at 365.666 kb on + strand, within IAI47_20575 at 365.667 kb on - strand, within IAI47_20575 at 365.667 kb on - strand, within IAI47_20575 at 365.729 kb on + strand, within IAI47_20575 at 365.730 kb on - strand, within IAI47_20575 at 365.803 kb on + strand at 365.803 kb on + strand at 365.804 kb on - strand at 365.804 kb on - strand at 365.843 kb on + strand at 365.843 kb on + strand at 365.843 kb on + strand at 365.843 kb on + strand at 365.843 kb on + strand at 365.843 kb on + strand at 365.843 kb on + strand at 365.843 kb on + strand at 365.844 kb on - strand at 365.844 kb on - strand at 365.844 kb on - strand at 365.844 kb on - strand at 365.844 kb on - strand at 365.844 kb on - strand at 365.859 kb on + strand at 365.859 kb on + strand at 365.859 kb on + strand at 365.859 kb on + strand at 365.859 kb on + strand at 365.859 kb on + strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.860 kb on - strand at 365.862 kb on + strand at 365.862 kb on + strand at 365.863 kb on - strand at 365.863 kb on - strand at 365.863 kb on - strand at 365.863 kb on - strand at 365.863 kb on - strand at 365.863 kb on - strand at 365.864 kb on + strand at 365.926 kb on + strand at 365.927 kb on - strand at 365.927 kb on - strand at 365.927 kb on - strand at 365.986 kb on + strand at 365.988 kb on + strand at 365.988 kb on + strand at 365.988 kb on + strand at 365.988 kb on + strand at 365.988 kb on + strand at 365.988 kb on + strand at 365.988 kb on + strand at 365.988 kb on + strand at 365.989 kb on - strand at 365.989 kb on - strand at 365.989 kb on - strand at 365.989 kb on - strand at 365.989 kb on - strand at 365.989 kb on - strand at 365.989 kb on - strand at 365.993 kb on + strand at 365.994 kb on - strand at 366.011 kb on + strand at 366.041 kb on + strand at 366.041 kb on + strand at 366.042 kb on - strand at 366.042 kb on - strand at 366.042 kb on - strand at 366.042 kb on - strand at 366.052 kb on + strand at 366.053 kb on - strand at 366.053 kb on - strand at 366.077 kb on + strand at 366.078 kb on - strand at 366.106 kb on + strand at 366.107 kb on - strand at 366.107 kb on - strand at 366.107 kb on - strand at 366.143 kb on + strand, within IAI47_20580 at 366.143 kb on + strand, within IAI47_20580 at 366.143 kb on + strand, within IAI47_20580 at 366.144 kb on - strand, within IAI47_20580 at 366.144 kb on - strand, within IAI47_20580 at 366.144 kb on - strand, within IAI47_20580 at 366.144 kb on - strand, within IAI47_20580 at 366.181 kb on - strand, within IAI47_20580 at 366.206 kb on - strand, within IAI47_20580 at 366.223 kb on - strand, within IAI47_20580 at 366.223 kb on - strand, within IAI47_20580 at 366.337 kb on + strand, within IAI47_20580 at 366.338 kb on - strand, within IAI47_20580 at 366.338 kb on - strand, within IAI47_20580 at 366.338 kb on - strand, within IAI47_20580 at 366.338 kb on - strand, within IAI47_20580 at 366.338 kb on - strand, within IAI47_20580 at 366.338 kb on - strand, within IAI47_20580 at 366.338 kb on - strand, within IAI47_20580 at 366.340 kb on + strand, within IAI47_20580 at 366.340 kb on + strand, within IAI47_20580 at 366.340 kb on + strand, within IAI47_20580 at 366.340 kb on + strand, within IAI47_20580 at 366.340 kb on + strand, within IAI47_20580 at 366.341 kb on - strand, within IAI47_20580 at 366.341 kb on - strand, within IAI47_20580 at 366.358 kb on + strand, within IAI47_20580 at 366.358 kb on + strand, within IAI47_20580 at 366.359 kb on - strand, within IAI47_20580 at 366.359 kb on - strand, within IAI47_20580 at 366.440 kb on + strand, within IAI47_20580 at 366.441 kb on - strand, within IAI47_20580 at 366.441 kb on - strand, within IAI47_20580 at 366.477 kb on + strand, within IAI47_20580 at 366.478 kb on - strand, within IAI47_20580 at 366.706 kb on + strand, within IAI47_20580 at 366.706 kb on + strand, within IAI47_20580 at 366.706 kb on + strand, within IAI47_20580 at 366.707 kb on - strand, within IAI47_20580 at 366.707 kb on - strand, within IAI47_20580 at 366.707 kb on - strand, within IAI47_20580 at 366.707 kb on - strand, within IAI47_20580 at 366.747 kb on + strand, within IAI47_20580 at 366.747 kb on + strand, within IAI47_20580 at 366.747 kb on + strand, within IAI47_20580 at 366.756 kb on + strand, within IAI47_20580 at 366.757 kb on - strand, within IAI47_20580 at 366.757 kb on - strand, within IAI47_20580 at 366.762 kb on + strand, within IAI47_20580 at 366.772 kb on + strand, within IAI47_20580 at 366.824 kb on + strand, within IAI47_20580 at 366.825 kb on - strand, within IAI47_20580 at 366.825 kb on - strand, within IAI47_20580 at 366.825 kb on - strand, within IAI47_20580 at 366.885 kb on + strand at 366.885 kb on + strand at 366.885 kb on + strand at 366.898 kb on - strand at 366.929 kb on + strand at 366.929 kb on + strand at 366.929 kb on + strand at 366.929 kb on + strand at 366.930 kb on - strand at 366.930 kb on - strand at 366.930 kb on - strand at 366.947 kb on + strand at 366.948 kb on - strand at 367.017 kb on + strand at 367.018 kb on - strand at 367.018 kb on - strand at 367.026 kb on + strand at 367.027 kb on - strand at 367.027 kb on - strand at 367.027 kb on - strand at 367.031 kb on + strand at 367.031 kb on + strand at 367.032 kb on - strand at 367.032 kb on - strand at 367.032 kb on - strand at 367.032 kb on - strand at 367.032 kb on - strand at 367.032 kb on - strand at 367.193 kb on + strand, within IAI47_20585 at 367.194 kb on - strand, within IAI47_20585 at 367.194 kb on - strand, within IAI47_20585 at 367.364 kb on - strand, within IAI47_20585 at 367.364 kb on - strand, within IAI47_20585 at 367.378 kb on - strand, within IAI47_20585 at 367.378 kb on - strand, within IAI47_20585 at 367.378 kb on - strand, within IAI47_20585 at 367.530 kb on + strand, within IAI47_20585 at 367.575 kb on + strand, within IAI47_20585 at 367.627 kb on + strand, within IAI47_20585 at 367.627 kb on + strand, within IAI47_20585 at 367.733 kb on + strand, within IAI47_20585 at 367.734 kb on - strand, within IAI47_20585 at 367.815 kb on + strand, within IAI47_20585 at 367.815 kb on + strand, within IAI47_20585 at 367.815 kb on + strand, within IAI47_20585 at 367.815 kb on + strand, within IAI47_20585 at 367.815 kb on + strand, within IAI47_20585 at 367.822 kb on + strand, within IAI47_20585 at 367.822 kb on + strand, within IAI47_20585 at 367.906 kb on + strand, within IAI47_20585 at 367.906 kb on + strand, within IAI47_20585 at 367.907 kb on - strand, within IAI47_20585 at 367.907 kb on - strand, within IAI47_20585 at 367.907 kb on - strand, within IAI47_20585
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=1hrs; Outgrowth=LBkan50 remove 365,062 - -0.2 365,062 - -1.1 365,062 - +2.6 365,062 - -0.4 365,082 - -1.9 365,106 + -1.1 365,107 - -0.6 365,131 + +0.9 365,131 + +0.6 365,131 + +0.8 365,131 + +0.7 365,131 + +1.0 365,132 - +0.3 365,132 - +0.7 365,132 - -0.2 365,132 - +1.4 365,197 - +0.9 365,197 - +0.1 365,197 - -0.1 365,235 + +0.4 365,236 - +1.6 365,251 + +0.0 365,259 - +0.7 365,259 - -0.2 365,259 - -1.7 365,358 + IAI47_20575 0.20 -0.9 365,358 + IAI47_20575 0.20 -0.1 365,384 + IAI47_20575 0.24 +0.3 365,384 + IAI47_20575 0.24 -0.3 365,384 + IAI47_20575 0.24 +0.1 365,384 + IAI47_20575 0.24 -0.4 365,384 + IAI47_20575 0.24 -0.5 365,384 + IAI47_20575 0.24 -0.2 365,384 + IAI47_20575 0.24 +0.4 365,384 + IAI47_20575 0.24 -0.5 365,384 + IAI47_20575 0.24 +0.2 365,385 - IAI47_20575 0.25 +0.3 365,385 - IAI47_20575 0.25 +0.7 365,385 - IAI47_20575 0.25 +1.6 365,386 + IAI47_20575 0.25 -1.5 365,386 + IAI47_20575 0.25 +1.1 365,386 + IAI47_20575 0.25 +1.0 365,386 + IAI47_20575 0.25 -0.9 365,387 - IAI47_20575 0.25 -0.8 365,387 - IAI47_20575 0.25 +0.7 365,387 - IAI47_20575 0.25 +0.6 365,428 + IAI47_20575 0.32 +0.3 365,428 + IAI47_20575 0.32 -0.9 365,429 - IAI47_20575 0.33 +1.2 365,429 - IAI47_20575 0.33 +0.8 365,463 - IAI47_20575 0.39 -0.7 365,463 - IAI47_20575 0.39 +0.6 365,464 + IAI47_20575 0.39 -0.1 365,464 + IAI47_20575 0.39 +1.1 365,464 + IAI47_20575 0.39 -1.0 365,464 + IAI47_20575 0.39 -0.5 365,465 - IAI47_20575 0.39 +2.0 365,465 - IAI47_20575 0.39 -0.4 365,465 - IAI47_20575 0.39 +0.2 365,465 - IAI47_20575 0.39 +1.0 365,465 - IAI47_20575 0.39 -0.1 365,522 + IAI47_20575 0.50 -0.0 365,522 + IAI47_20575 0.50 -1.3 365,522 + IAI47_20575 0.50 -0.4 365,522 + IAI47_20575 0.50 -0.9 365,523 - IAI47_20575 0.50 -0.9 365,523 - IAI47_20575 0.50 +2.3 365,524 + IAI47_20575 0.50 +0.4 365,524 + IAI47_20575 0.50 +1.8 365,524 + IAI47_20575 0.50 +0.1 365,525 - IAI47_20575 0.50 +0.4 365,525 - IAI47_20575 0.50 +1.0 365,661 - IAI47_20575 0.75 -0.7 365,661 - IAI47_20575 0.75 -0.4 365,666 + IAI47_20575 0.76 -1.5 365,667 - IAI47_20575 0.76 +0.2 365,667 - IAI47_20575 0.76 -1.1 365,729 + IAI47_20575 0.87 -1.5 365,730 - IAI47_20575 0.87 +0.0 365,803 + -0.1 365,803 + +0.0 365,804 - -0.1 365,804 - +1.4 365,843 + +0.5 365,843 + -1.9 365,843 + +1.8 365,843 + -0.9 365,843 + -0.3 365,843 + -0.9 365,843 + -0.4 365,843 + +0.6 365,844 - +0.5 365,844 - +2.3 365,844 - -0.2 365,844 - +1.5 365,844 - -1.8 365,844 - +0.0 365,859 + +1.5 365,859 + -0.7 365,859 + -1.0 365,859 + +0.6 365,859 + +0.1 365,859 + +0.3 365,860 - +0.2 365,860 - +1.3 365,860 - -0.8 365,860 - -0.3 365,860 - +1.3 365,860 - +0.3 365,860 - -0.1 365,860 - +0.3 365,860 - +1.5 365,860 - -0.1 365,860 - +0.3 365,862 + +1.4 365,862 + +0.6 365,863 - -0.2 365,863 - -0.6 365,863 - +0.4 365,863 - -0.3 365,863 - +1.1 365,863 - +0.8 365,864 + +0.4 365,926 + -0.6 365,927 - -1.0 365,927 - +2.5 365,927 - +0.6 365,986 + -1.1 365,988 + -1.5 365,988 + +1.0 365,988 + -0.4 365,988 + +0.7 365,988 + +1.1 365,988 + +0.6 365,988 + +1.0 365,988 + +0.4 365,989 - +1.5 365,989 - -0.3 365,989 - +0.3 365,989 - -0.1 365,989 - -0.4 365,989 - +0.4 365,989 - +1.2 365,993 + +0.8 365,994 - +3.2 366,011 + -0.1 366,041 + -1.6 366,041 + -0.1 366,042 - +1.0 366,042 - +1.5 366,042 - +1.1 366,042 - +0.2 366,052 + +0.7 366,053 - +1.3 366,053 - -0.4 366,077 + +0.1 366,078 - +0.4 366,106 + +1.6 366,107 - +0.2 366,107 - -1.5 366,107 - -0.3 366,143 + IAI47_20580 0.10 +0.6 366,143 + IAI47_20580 0.10 +0.6 366,143 + IAI47_20580 0.10 +0.4 366,144 - IAI47_20580 0.10 -1.2 366,144 - IAI47_20580 0.10 +0.1 366,144 - IAI47_20580 0.10 +1.7 366,144 - IAI47_20580 0.10 -0.1 366,181 - IAI47_20580 0.15 -0.3 366,206 - IAI47_20580 0.17 +0.2 366,223 - IAI47_20580 0.19 -0.1 366,223 - IAI47_20580 0.19 +0.5 366,337 + IAI47_20580 0.32 +0.0 366,338 - IAI47_20580 0.32 -0.1 366,338 - IAI47_20580 0.32 -0.1 366,338 - IAI47_20580 0.32 +0.6 366,338 - IAI47_20580 0.32 +0.0 366,338 - IAI47_20580 0.32 -0.9 366,338 - IAI47_20580 0.32 +0.1 366,338 - IAI47_20580 0.32 -1.2 366,340 + IAI47_20580 0.33 -0.2 366,340 + IAI47_20580 0.33 -1.3 366,340 + IAI47_20580 0.33 -0.2 366,340 + IAI47_20580 0.33 -0.2 366,340 + IAI47_20580 0.33 -0.4 366,341 - IAI47_20580 0.33 +0.7 366,341 - IAI47_20580 0.33 -0.5 366,358 + IAI47_20580 0.35 -1.1 366,358 + IAI47_20580 0.35 -0.5 366,359 - IAI47_20580 0.35 +0.4 366,359 - IAI47_20580 0.35 -0.9 366,440 + IAI47_20580 0.44 +0.9 366,441 - IAI47_20580 0.44 -0.7 366,441 - IAI47_20580 0.44 +0.7 366,477 + IAI47_20580 0.48 +0.4 366,478 - IAI47_20580 0.48 -0.9 366,706 + IAI47_20580 0.74 +0.3 366,706 + IAI47_20580 0.74 -1.0 366,706 + IAI47_20580 0.74 +1.4 366,707 - IAI47_20580 0.74 -0.7 366,707 - IAI47_20580 0.74 +0.4 366,707 - IAI47_20580 0.74 +0.9 366,707 - IAI47_20580 0.74 +1.2 366,747 + IAI47_20580 0.79 -1.1 366,747 + IAI47_20580 0.79 +0.0 366,747 + IAI47_20580 0.79 +1.1 366,756 + IAI47_20580 0.80 +0.2 366,757 - IAI47_20580 0.80 -0.7 366,757 - IAI47_20580 0.80 +1.1 366,762 + IAI47_20580 0.81 +1.5 366,772 + IAI47_20580 0.82 +0.5 366,824 + IAI47_20580 0.88 +0.9 366,825 - IAI47_20580 0.88 -1.4 366,825 - IAI47_20580 0.88 +1.4 366,825 - IAI47_20580 0.88 -1.3 366,885 + -0.9 366,885 + -1.9 366,885 + +1.7 366,898 - -0.6 366,929 + -0.9 366,929 + -0.3 366,929 + -1.4 366,929 + +0.4 366,930 - -0.8 366,930 - +0.5 366,930 - +2.5 366,947 + -1.5 366,948 - -1.1 367,017 + -1.8 367,018 - +1.0 367,018 - -0.2 367,026 + +1.4 367,027 - +0.0 367,027 - +0.8 367,027 - +0.1 367,031 + -0.1 367,031 + -0.9 367,032 - +0.0 367,032 - +0.4 367,032 - -1.3 367,032 - +0.4 367,032 - +0.5 367,032 - -1.4 367,193 + IAI47_20585 0.15 -1.9 367,194 - IAI47_20585 0.15 +0.1 367,194 - IAI47_20585 0.15 +1.1 367,364 - IAI47_20585 0.26 -0.5 367,364 - IAI47_20585 0.26 +0.4 367,378 - IAI47_20585 0.26 +0.9 367,378 - IAI47_20585 0.26 -0.2 367,378 - IAI47_20585 0.26 +1.3 367,530 + IAI47_20585 0.36 -1.0 367,575 + IAI47_20585 0.38 -1.0 367,627 + IAI47_20585 0.42 -0.6 367,627 + IAI47_20585 0.42 -1.3 367,733 + IAI47_20585 0.48 +0.6 367,734 - IAI47_20585 0.48 +2.0 367,815 + IAI47_20585 0.53 -0.6 367,815 + IAI47_20585 0.53 +2.1 367,815 + IAI47_20585 0.53 -0.7 367,815 + IAI47_20585 0.53 -0.3 367,815 + IAI47_20585 0.53 -0.9 367,822 + IAI47_20585 0.53 -0.6 367,822 + IAI47_20585 0.53 -1.3 367,906 + IAI47_20585 0.59 +1.1 367,906 + IAI47_20585 0.59 +0.0 367,907 - IAI47_20585 0.59 +0.5 367,907 - IAI47_20585 0.59 +1.4 367,907 - IAI47_20585 0.59 +0.4
Or see this region's nucleotide sequence