Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c34490 and PGA1_c34500 overlap by 1 nucleotides PGA1_c34500 and PGA1_c34510 are separated by 135 nucleotides PGA1_c34510 and PGA1_c34520 are separated by 20 nucleotides PGA1_c34520 and PGA1_c34530 are separated by 126 nucleotides
PGA1_c34490: PGA1_c34490 - 3-deoxy-manno-octulosonate cytidylyltransferase KdsB, at 3,616,656 to 3,617,456
_c34490
PGA1_c34500: PGA1_c34500 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 3,617,456 to 3,618,253
_c34500
PGA1_c34510: PGA1_c34510 - hypothetical protein, at 3,618,389 to 3,619,210
_c34510
PGA1_c34520: PGA1_c34520 - alpha-ketoglutarate-dependent dioxygenase AlkB, at 3,619,231 to 3,619,920
_c34520
PGA1_c34530: PGA1_c34530 - chaperone protein DnaK, at 3,620,047 to 3,621,966
_c34530
Position (kb)
3618
3619
3620 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3618.265 kb on - strand at 3618.275 kb on - strand at 3618.279 kb on - strand at 3618.289 kb on - strand at 3618.299 kb on + strand at 3618.323 kb on - strand at 3618.362 kb on - strand at 3618.367 kb on - strand at 3618.369 kb on + strand at 3618.369 kb on + strand at 3618.369 kb on + strand at 3618.369 kb on + strand at 3618.369 kb on + strand at 3618.377 kb on - strand at 3618.377 kb on - strand at 3618.377 kb on - strand at 3618.416 kb on + strand at 3618.416 kb on + strand at 3618.416 kb on + strand at 3618.424 kb on - strand at 3618.424 kb on - strand at 3618.447 kb on - strand at 3618.447 kb on - strand at 3618.509 kb on - strand, within PGA1_c34510 at 3618.520 kb on + strand, within PGA1_c34510 at 3618.520 kb on + strand, within PGA1_c34510 at 3618.524 kb on - strand, within PGA1_c34510 at 3618.526 kb on + strand, within PGA1_c34510 at 3618.528 kb on - strand, within PGA1_c34510 at 3618.528 kb on - strand, within PGA1_c34510 at 3618.582 kb on + strand, within PGA1_c34510 at 3618.590 kb on - strand, within PGA1_c34510 at 3618.640 kb on + strand, within PGA1_c34510 at 3618.646 kb on + strand, within PGA1_c34510 at 3618.646 kb on + strand, within PGA1_c34510 at 3618.646 kb on + strand, within PGA1_c34510 at 3618.648 kb on + strand, within PGA1_c34510 at 3618.654 kb on - strand, within PGA1_c34510 at 3618.654 kb on - strand, within PGA1_c34510 at 3618.654 kb on - strand, within PGA1_c34510 at 3618.654 kb on - strand, within PGA1_c34510 at 3618.654 kb on - strand, within PGA1_c34510 at 3618.654 kb on - strand, within PGA1_c34510 at 3618.654 kb on - strand, within PGA1_c34510 at 3618.654 kb on - strand, within PGA1_c34510 at 3618.680 kb on + strand, within PGA1_c34510 at 3618.680 kb on - strand, within PGA1_c34510 at 3618.688 kb on - strand, within PGA1_c34510 at 3618.690 kb on + strand, within PGA1_c34510 at 3618.710 kb on + strand, within PGA1_c34510 at 3618.710 kb on + strand, within PGA1_c34510 at 3618.715 kb on - strand, within PGA1_c34510 at 3618.730 kb on - strand, within PGA1_c34510 at 3618.737 kb on - strand, within PGA1_c34510 at 3618.767 kb on - strand, within PGA1_c34510 at 3618.784 kb on - strand, within PGA1_c34510 at 3618.854 kb on + strand, within PGA1_c34510 at 3618.854 kb on + strand, within PGA1_c34510 at 3618.854 kb on + strand, within PGA1_c34510 at 3618.854 kb on + strand, within PGA1_c34510 at 3618.854 kb on + strand, within PGA1_c34510 at 3618.854 kb on + strand, within PGA1_c34510 at 3618.862 kb on - strand, within PGA1_c34510 at 3618.862 kb on - strand, within PGA1_c34510 at 3618.862 kb on - strand, within PGA1_c34510 at 3618.918 kb on - strand, within PGA1_c34510 at 3618.977 kb on + strand, within PGA1_c34510 at 3619.008 kb on + strand, within PGA1_c34510 at 3619.008 kb on + strand, within PGA1_c34510 at 3619.008 kb on + strand, within PGA1_c34510 at 3619.008 kb on + strand, within PGA1_c34510 at 3619.008 kb on + strand, within PGA1_c34510 at 3619.016 kb on - strand, within PGA1_c34510 at 3619.016 kb on - strand, within PGA1_c34510 at 3619.016 kb on - strand, within PGA1_c34510 at 3619.016 kb on - strand, within PGA1_c34510 at 3619.016 kb on - strand, within PGA1_c34510 at 3619.068 kb on - strand, within PGA1_c34510 at 3619.084 kb on + strand, within PGA1_c34510 at 3619.212 kb on - strand at 3619.212 kb on - strand at 3619.212 kb on - strand at 3619.256 kb on - strand at 3619.276 kb on - strand at 3619.276 kb on - strand at 3619.281 kb on + strand at 3619.308 kb on - strand, within PGA1_c34520 at 3619.350 kb on + strand, within PGA1_c34520 at 3619.376 kb on + strand, within PGA1_c34520 at 3619.376 kb on + strand, within PGA1_c34520 at 3619.376 kb on + strand, within PGA1_c34520 at 3619.376 kb on + strand, within PGA1_c34520 at 3619.376 kb on + strand, within PGA1_c34520 at 3619.384 kb on - strand, within PGA1_c34520 at 3619.384 kb on - strand, within PGA1_c34520 at 3619.417 kb on - strand, within PGA1_c34520 at 3619.480 kb on - strand, within PGA1_c34520 at 3619.480 kb on - strand, within PGA1_c34520 at 3619.483 kb on + strand, within PGA1_c34520 at 3619.487 kb on + strand, within PGA1_c34520 at 3619.492 kb on + strand, within PGA1_c34520 at 3619.501 kb on + strand, within PGA1_c34520 at 3619.543 kb on + strand, within PGA1_c34520 at 3619.543 kb on + strand, within PGA1_c34520 at 3619.551 kb on - strand, within PGA1_c34520 at 3619.562 kb on + strand, within PGA1_c34520 at 3619.562 kb on + strand, within PGA1_c34520 at 3619.595 kb on + strand, within PGA1_c34520 at 3619.613 kb on - strand, within PGA1_c34520 at 3619.623 kb on + strand, within PGA1_c34520 at 3619.623 kb on + strand, within PGA1_c34520 at 3619.631 kb on - strand, within PGA1_c34520 at 3619.715 kb on + strand, within PGA1_c34520 at 3619.718 kb on - strand, within PGA1_c34520 at 3619.723 kb on - strand, within PGA1_c34520 at 3619.723 kb on - strand, within PGA1_c34520 at 3619.725 kb on + strand, within PGA1_c34520 at 3619.735 kb on + strand, within PGA1_c34520 at 3619.762 kb on - strand, within PGA1_c34520 at 3619.793 kb on + strand, within PGA1_c34520 at 3619.801 kb on - strand, within PGA1_c34520 at 3619.803 kb on + strand, within PGA1_c34520 at 3619.811 kb on - strand, within PGA1_c34520 at 3619.811 kb on - strand, within PGA1_c34520 at 3619.811 kb on - strand, within PGA1_c34520 at 3619.836 kb on + strand, within PGA1_c34520 at 3619.836 kb on + strand, within PGA1_c34520 at 3619.836 kb on - strand, within PGA1_c34520 at 3619.844 kb on - strand, within PGA1_c34520 at 3619.844 kb on - strand, within PGA1_c34520 at 3619.854 kb on + strand at 3619.854 kb on + strand at 3619.940 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 3,618,265 - -0.7 3,618,275 - -0.4 3,618,279 - +0.1 3,618,289 - -1.9 3,618,299 + +0.3 3,618,323 - -0.6 3,618,362 - +0.0 3,618,367 - +0.9 3,618,369 + -0.6 3,618,369 + +0.7 3,618,369 + +0.7 3,618,369 + -0.5 3,618,369 + +0.2 3,618,377 - -0.6 3,618,377 - -0.6 3,618,377 - +0.4 3,618,416 + -0.9 3,618,416 + -0.6 3,618,416 + +0.2 3,618,424 - -0.4 3,618,424 - +0.5 3,618,447 - +0.3 3,618,447 - +0.3 3,618,509 - PGA1_c34510 0.15 -0.2 3,618,520 + PGA1_c34510 0.16 +0.5 3,618,520 + PGA1_c34510 0.16 +0.2 3,618,524 - PGA1_c34510 0.16 +0.5 3,618,526 + PGA1_c34510 0.17 -0.5 3,618,528 - PGA1_c34510 0.17 +0.8 3,618,528 - PGA1_c34510 0.17 +0.0 3,618,582 + PGA1_c34510 0.23 +0.4 3,618,590 - PGA1_c34510 0.24 +0.6 3,618,640 + PGA1_c34510 0.31 +0.5 3,618,646 + PGA1_c34510 0.31 +0.3 3,618,646 + PGA1_c34510 0.31 -0.3 3,618,646 + PGA1_c34510 0.31 +0.2 3,618,648 + PGA1_c34510 0.32 +0.5 3,618,654 - PGA1_c34510 0.32 +2.1 3,618,654 - PGA1_c34510 0.32 +0.2 3,618,654 - PGA1_c34510 0.32 +0.9 3,618,654 - PGA1_c34510 0.32 -2.1 3,618,654 - PGA1_c34510 0.32 -0.0 3,618,654 - PGA1_c34510 0.32 +0.3 3,618,654 - PGA1_c34510 0.32 -0.8 3,618,654 - PGA1_c34510 0.32 +0.3 3,618,680 + PGA1_c34510 0.35 -1.0 3,618,680 - PGA1_c34510 0.35 +0.9 3,618,688 - PGA1_c34510 0.36 +0.4 3,618,690 + PGA1_c34510 0.37 -0.1 3,618,710 + PGA1_c34510 0.39 +1.6 3,618,710 + PGA1_c34510 0.39 -0.6 3,618,715 - PGA1_c34510 0.40 +1.0 3,618,730 - PGA1_c34510 0.41 +0.2 3,618,737 - PGA1_c34510 0.42 +1.7 3,618,767 - PGA1_c34510 0.46 +1.6 3,618,784 - PGA1_c34510 0.48 +0.4 3,618,854 + PGA1_c34510 0.57 +1.1 3,618,854 + PGA1_c34510 0.57 +0.1 3,618,854 + PGA1_c34510 0.57 +0.2 3,618,854 + PGA1_c34510 0.57 -0.3 3,618,854 + PGA1_c34510 0.57 +0.5 3,618,854 + PGA1_c34510 0.57 +0.1 3,618,862 - PGA1_c34510 0.58 -0.1 3,618,862 - PGA1_c34510 0.58 +0.4 3,618,862 - PGA1_c34510 0.58 +0.6 3,618,918 - PGA1_c34510 0.64 +0.2 3,618,977 + PGA1_c34510 0.72 +0.4 3,619,008 + PGA1_c34510 0.75 -0.2 3,619,008 + PGA1_c34510 0.75 -0.0 3,619,008 + PGA1_c34510 0.75 +1.0 3,619,008 + PGA1_c34510 0.75 +0.4 3,619,008 + PGA1_c34510 0.75 +0.5 3,619,016 - PGA1_c34510 0.76 +0.2 3,619,016 - PGA1_c34510 0.76 +0.7 3,619,016 - PGA1_c34510 0.76 +0.2 3,619,016 - PGA1_c34510 0.76 -0.5 3,619,016 - PGA1_c34510 0.76 -1.1 3,619,068 - PGA1_c34510 0.83 +0.0 3,619,084 + PGA1_c34510 0.85 +0.4 3,619,212 - -0.7 3,619,212 - -0.2 3,619,212 - +0.7 3,619,256 - -0.2 3,619,276 - +0.7 3,619,276 - -0.7 3,619,281 + +0.0 3,619,308 - PGA1_c34520 0.11 +0.1 3,619,350 + PGA1_c34520 0.17 -1.1 3,619,376 + PGA1_c34520 0.21 +0.4 3,619,376 + PGA1_c34520 0.21 -0.7 3,619,376 + PGA1_c34520 0.21 -2.7 3,619,376 + PGA1_c34520 0.21 -0.8 3,619,376 + PGA1_c34520 0.21 +0.4 3,619,384 - PGA1_c34520 0.22 -1.3 3,619,384 - PGA1_c34520 0.22 +1.3 3,619,417 - PGA1_c34520 0.27 -0.2 3,619,480 - PGA1_c34520 0.36 -0.0 3,619,480 - PGA1_c34520 0.36 -0.7 3,619,483 + PGA1_c34520 0.37 +0.2 3,619,487 + PGA1_c34520 0.37 -1.1 3,619,492 + PGA1_c34520 0.38 +0.8 3,619,501 + PGA1_c34520 0.39 +0.4 3,619,543 + PGA1_c34520 0.45 +1.3 3,619,543 + PGA1_c34520 0.45 -0.9 3,619,551 - PGA1_c34520 0.46 -0.2 3,619,562 + PGA1_c34520 0.48 +0.4 3,619,562 + PGA1_c34520 0.48 +1.1 3,619,595 + PGA1_c34520 0.53 -0.7 3,619,613 - PGA1_c34520 0.55 +0.1 3,619,623 + PGA1_c34520 0.57 +0.2 3,619,623 + PGA1_c34520 0.57 +0.6 3,619,631 - PGA1_c34520 0.58 +0.1 3,619,715 + PGA1_c34520 0.70 -1.0 3,619,718 - PGA1_c34520 0.71 +1.1 3,619,723 - PGA1_c34520 0.71 -1.7 3,619,723 - PGA1_c34520 0.71 -0.3 3,619,725 + PGA1_c34520 0.72 +0.7 3,619,735 + PGA1_c34520 0.73 +0.6 3,619,762 - PGA1_c34520 0.77 +0.2 3,619,793 + PGA1_c34520 0.81 +0.2 3,619,801 - PGA1_c34520 0.83 +0.3 3,619,803 + PGA1_c34520 0.83 +0.0 3,619,811 - PGA1_c34520 0.84 -0.4 3,619,811 - PGA1_c34520 0.84 +0.2 3,619,811 - PGA1_c34520 0.84 +0.1 3,619,836 + PGA1_c34520 0.88 -0.3 3,619,836 + PGA1_c34520 0.88 +0.9 3,619,836 - PGA1_c34520 0.88 +0.9 3,619,844 - PGA1_c34520 0.89 +1.0 3,619,844 - PGA1_c34520 0.89 +1.9 3,619,854 + -0.5 3,619,854 + +0.4 3,619,940 - -0.2
Or see this region's nucleotide sequence