Experiment: m.b. Cholin acetate 15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03420 and PGA1_c03430 overlap by 8 nucleotides PGA1_c03430 and PGA1_c03440 are separated by 119 nucleotides PGA1_c03440 and PGA1_c03450 are separated by 22 nucleotides
PGA1_c03420: PGA1_c03420 - putative C-5 sterol desaturase, at 328,918 to 329,847
_c03420
PGA1_c03430: PGA1_c03430 - hypothetical protein, at 329,840 to 330,280
_c03430
PGA1_c03440: PGA1_c03440 - disulfide interchange protein tlpA-like protein, at 330,400 to 330,987
_c03440
PGA1_c03450: PGA1_c03450 - argininosuccinate lyase ArgH, at 331,010 to 332,380
_c03450
Position (kb)
329
330
331 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 328.841 kb on + strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.849 kb on - strand at 328.849 kb on - strand at 328.915 kb on + strand at 328.916 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.921 kb on + strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.942 kb on - strand at 328.944 kb on + strand at 328.949 kb on + strand at 328.952 kb on - strand at 328.980 kb on + strand at 328.980 kb on + strand at 328.988 kb on - strand at 329.011 kb on + strand, within PGA1_c03420 at 329.099 kb on - strand, within PGA1_c03420 at 329.115 kb on + strand, within PGA1_c03420 at 329.135 kb on - strand, within PGA1_c03420 at 329.139 kb on + strand, within PGA1_c03420 at 329.143 kb on - strand, within PGA1_c03420 at 329.154 kb on - strand, within PGA1_c03420 at 329.169 kb on - strand, within PGA1_c03420 at 329.169 kb on - strand, within PGA1_c03420 at 329.203 kb on - strand, within PGA1_c03420 at 329.217 kb on + strand, within PGA1_c03420 at 329.217 kb on + strand, within PGA1_c03420 at 329.269 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.284 kb on + strand, within PGA1_c03420 at 329.295 kb on + strand, within PGA1_c03420 at 329.295 kb on + strand, within PGA1_c03420 at 329.303 kb on - strand, within PGA1_c03420 at 329.305 kb on + strand, within PGA1_c03420 at 329.324 kb on + strand, within PGA1_c03420 at 329.344 kb on - strand, within PGA1_c03420 at 329.358 kb on - strand, within PGA1_c03420 at 329.388 kb on + strand, within PGA1_c03420 at 329.404 kb on + strand, within PGA1_c03420 at 329.412 kb on - strand, within PGA1_c03420 at 329.412 kb on - strand, within PGA1_c03420 at 329.441 kb on - strand, within PGA1_c03420 at 329.447 kb on + strand, within PGA1_c03420 at 329.514 kb on + strand, within PGA1_c03420 at 329.520 kb on - strand, within PGA1_c03420 at 329.522 kb on + strand, within PGA1_c03420 at 329.547 kb on + strand, within PGA1_c03420 at 329.552 kb on + strand, within PGA1_c03420 at 329.558 kb on - strand, within PGA1_c03420 at 329.558 kb on - strand, within PGA1_c03420 at 329.606 kb on + strand, within PGA1_c03420 at 329.629 kb on - strand, within PGA1_c03420 at 329.682 kb on + strand, within PGA1_c03420 at 329.714 kb on - strand, within PGA1_c03420 at 329.721 kb on - strand, within PGA1_c03420 at 329.833 kb on - strand at 329.857 kb on - strand at 329.871 kb on - strand at 329.871 kb on - strand at 329.871 kb on - strand at 329.882 kb on - strand at 329.884 kb on + strand, within PGA1_c03430 at 329.892 kb on - strand, within PGA1_c03430 at 329.921 kb on + strand, within PGA1_c03430 at 329.994 kb on + strand, within PGA1_c03430 at 329.994 kb on + strand, within PGA1_c03430 at 330.007 kb on + strand, within PGA1_c03430 at 330.020 kb on - strand, within PGA1_c03430 at 330.022 kb on + strand, within PGA1_c03430 at 330.030 kb on - strand, within PGA1_c03430 at 330.057 kb on + strand, within PGA1_c03430 at 330.065 kb on - strand, within PGA1_c03430 at 330.065 kb on - strand, within PGA1_c03430 at 330.067 kb on + strand, within PGA1_c03430 at 330.082 kb on - strand, within PGA1_c03430 at 330.094 kb on + strand, within PGA1_c03430 at 330.097 kb on - strand, within PGA1_c03430 at 330.103 kb on + strand, within PGA1_c03430 at 330.155 kb on + strand, within PGA1_c03430 at 330.171 kb on - strand, within PGA1_c03430 at 330.172 kb on - strand, within PGA1_c03430 at 330.196 kb on + strand, within PGA1_c03430 at 330.198 kb on - strand, within PGA1_c03430 at 330.250 kb on + strand at 330.287 kb on + strand at 330.332 kb on - strand at 330.333 kb on - strand at 330.367 kb on + strand at 330.375 kb on - strand at 330.385 kb on + strand at 330.393 kb on - strand at 330.402 kb on + strand at 330.402 kb on + strand at 330.405 kb on - strand at 330.801 kb on + strand, within PGA1_c03440 at 330.969 kb on - strand at 330.982 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 15 mM remove 328,841 + -1.9 328,841 + -0.4 328,841 + -0.6 328,841 + +0.5 328,849 - -0.3 328,849 - -1.0 328,915 + +0.9 328,916 - +0.1 328,918 - +0.7 328,918 - +0.6 328,918 - +0.9 328,918 - +0.6 328,921 + +0.3 328,923 - +0.8 328,923 - +0.1 328,923 - -0.2 328,923 - +0.0 328,923 - -0.2 328,942 - +0.3 328,944 + -1.8 328,949 + -0.3 328,952 - -0.6 328,980 + -0.2 328,980 + +0.5 328,988 - +0.5 329,011 + PGA1_c03420 0.10 +0.6 329,099 - PGA1_c03420 0.19 +0.7 329,115 + PGA1_c03420 0.21 -0.3 329,135 - PGA1_c03420 0.23 -0.2 329,139 + PGA1_c03420 0.24 +0.4 329,143 - PGA1_c03420 0.24 +2.1 329,154 - PGA1_c03420 0.25 +0.4 329,169 - PGA1_c03420 0.27 +0.9 329,169 - PGA1_c03420 0.27 +0.2 329,203 - PGA1_c03420 0.31 +0.5 329,217 + PGA1_c03420 0.32 -0.0 329,217 + PGA1_c03420 0.32 +0.5 329,269 + PGA1_c03420 0.38 -0.0 329,274 + PGA1_c03420 0.38 +0.6 329,274 + PGA1_c03420 0.38 -0.1 329,274 + PGA1_c03420 0.38 -0.7 329,284 + PGA1_c03420 0.39 +0.4 329,295 + PGA1_c03420 0.41 -0.0 329,295 + PGA1_c03420 0.41 +0.4 329,303 - PGA1_c03420 0.41 +0.6 329,305 + PGA1_c03420 0.42 -0.2 329,324 + PGA1_c03420 0.44 -0.4 329,344 - PGA1_c03420 0.46 +0.5 329,358 - PGA1_c03420 0.47 +0.2 329,388 + PGA1_c03420 0.51 -1.6 329,404 + PGA1_c03420 0.52 +0.3 329,412 - PGA1_c03420 0.53 -0.3 329,412 - PGA1_c03420 0.53 +0.2 329,441 - PGA1_c03420 0.56 +0.4 329,447 + PGA1_c03420 0.57 +0.5 329,514 + PGA1_c03420 0.64 -0.2 329,520 - PGA1_c03420 0.65 +0.2 329,522 + PGA1_c03420 0.65 +0.4 329,547 + PGA1_c03420 0.68 +0.1 329,552 + PGA1_c03420 0.68 -0.3 329,558 - PGA1_c03420 0.69 -0.2 329,558 - PGA1_c03420 0.69 +0.1 329,606 + PGA1_c03420 0.74 +0.8 329,629 - PGA1_c03420 0.76 -0.0 329,682 + PGA1_c03420 0.82 +0.3 329,714 - PGA1_c03420 0.86 +0.3 329,721 - PGA1_c03420 0.86 +0.9 329,833 - -0.8 329,857 - +0.1 329,871 - -0.0 329,871 - -0.1 329,871 - +1.1 329,882 - +0.1 329,884 + PGA1_c03430 0.10 -0.4 329,892 - PGA1_c03430 0.12 -0.2 329,921 + PGA1_c03430 0.18 +1.1 329,994 + PGA1_c03430 0.35 -0.5 329,994 + PGA1_c03430 0.35 +0.1 330,007 + PGA1_c03430 0.38 +0.1 330,020 - PGA1_c03430 0.41 +0.3 330,022 + PGA1_c03430 0.41 +0.1 330,030 - PGA1_c03430 0.43 +0.3 330,057 + PGA1_c03430 0.49 -0.8 330,065 - PGA1_c03430 0.51 -0.2 330,065 - PGA1_c03430 0.51 -0.4 330,067 + PGA1_c03430 0.51 +0.8 330,082 - PGA1_c03430 0.55 -0.8 330,094 + PGA1_c03430 0.58 -1.4 330,097 - PGA1_c03430 0.58 +0.1 330,103 + PGA1_c03430 0.60 +0.0 330,155 + PGA1_c03430 0.71 -0.4 330,171 - PGA1_c03430 0.75 +0.0 330,172 - PGA1_c03430 0.75 +0.4 330,196 + PGA1_c03430 0.81 -0.5 330,198 - PGA1_c03430 0.81 +0.1 330,250 + +0.8 330,287 + -0.7 330,332 - -0.9 330,333 - +0.6 330,367 + -0.4 330,375 - +0.4 330,385 + -2.1 330,393 - +0.5 330,402 + +0.2 330,402 + +0.6 330,405 - +0.1 330,801 + PGA1_c03440 0.68 -1.4 330,969 - -0.9 330,982 + +0.5
Or see this region's nucleotide sequence