Experiment: N2 fixation, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04465 and MMP_RS04470 are separated by 17 nucleotides MMP_RS04470 and MMP_RS04475 overlap by 35 nucleotides MMP_RS04475 and MMP_RS04480 are separated by 575 nucleotides
MMP_RS04465: MMP_RS04465 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 848,539 to 849,981
_RS04465
MMP_RS04470: MMP_RS04470 - nitrogenase, at 849,999 to 851,375
_RS04470
MMP_RS04475: MMP_RS04475 - nitrogen fixation, at 851,341 to 851,661
_RS04475
MMP_RS04480: MMP_RS04480 - lysine 2 3-aminomutase, at 852,237 to 853,538
_RS04480
Position (kb)
849
850
851
852 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 849.013 kb on + strand, within MMP_RS04465 at 849.270 kb on + strand, within MMP_RS04465 at 849.361 kb on + strand, within MMP_RS04465 at 849.363 kb on + strand, within MMP_RS04465 at 849.388 kb on - strand, within MMP_RS04465 at 849.463 kb on + strand, within MMP_RS04465 at 849.555 kb on - strand, within MMP_RS04465 at 849.594 kb on - strand, within MMP_RS04465 at 849.595 kb on + strand, within MMP_RS04465 at 849.609 kb on + strand, within MMP_RS04465 at 849.610 kb on - strand, within MMP_RS04465 at 849.610 kb on - strand, within MMP_RS04465 at 849.642 kb on + strand, within MMP_RS04465 at 849.673 kb on + strand, within MMP_RS04465 at 849.746 kb on - strand, within MMP_RS04465 at 849.746 kb on - strand, within MMP_RS04465 at 849.747 kb on + strand, within MMP_RS04465 at 849.747 kb on + strand, within MMP_RS04465 at 849.779 kb on - strand, within MMP_RS04465 at 849.779 kb on - strand, within MMP_RS04465 at 849.780 kb on + strand, within MMP_RS04465 at 849.810 kb on + strand, within MMP_RS04465 at 849.810 kb on + strand, within MMP_RS04465 at 849.810 kb on + strand, within MMP_RS04465 at 849.812 kb on - strand, within MMP_RS04465 at 849.812 kb on - strand, within MMP_RS04465 at 849.935 kb on - strand at 849.935 kb on - strand at 849.935 kb on - strand at 849.936 kb on + strand at 849.996 kb on + strand at 849.999 kb on + strand at 850.061 kb on + strand at 850.126 kb on + strand at 850.126 kb on + strand at 850.204 kb on - strand, within MMP_RS04470 at 850.207 kb on - strand, within MMP_RS04470 at 850.207 kb on - strand, within MMP_RS04470 at 850.270 kb on - strand, within MMP_RS04470 at 850.283 kb on - strand, within MMP_RS04470 at 850.284 kb on + strand, within MMP_RS04470 at 850.284 kb on + strand, within MMP_RS04470 at 850.360 kb on - strand, within MMP_RS04470 at 850.394 kb on - strand, within MMP_RS04470 at 850.395 kb on + strand, within MMP_RS04470 at 850.395 kb on + strand, within MMP_RS04470 at 850.395 kb on + strand, within MMP_RS04470 at 850.400 kb on - strand, within MMP_RS04470 at 850.401 kb on + strand, within MMP_RS04470 at 850.408 kb on - strand, within MMP_RS04470 at 850.408 kb on - strand, within MMP_RS04470 at 850.408 kb on - strand, within MMP_RS04470 at 850.409 kb on + strand, within MMP_RS04470 at 850.409 kb on + strand, within MMP_RS04470 at 850.480 kb on - strand, within MMP_RS04470 at 850.481 kb on + strand, within MMP_RS04470 at 850.553 kb on + strand, within MMP_RS04470 at 850.562 kb on + strand, within MMP_RS04470 at 850.586 kb on - strand, within MMP_RS04470 at 850.587 kb on + strand, within MMP_RS04470 at 850.587 kb on + strand, within MMP_RS04470 at 850.637 kb on - strand, within MMP_RS04470 at 850.805 kb on - strand, within MMP_RS04470 at 850.838 kb on + strand, within MMP_RS04470 at 850.841 kb on - strand, within MMP_RS04470 at 850.841 kb on - strand, within MMP_RS04470 at 850.934 kb on + strand, within MMP_RS04470 at 851.032 kb on - strand, within MMP_RS04470 at 851.037 kb on + strand, within MMP_RS04470 at 851.052 kb on + strand, within MMP_RS04470 at 851.066 kb on + strand, within MMP_RS04470 at 851.126 kb on + strand, within MMP_RS04470 at 851.180 kb on - strand, within MMP_RS04470 at 851.199 kb on - strand, within MMP_RS04470 at 851.205 kb on + strand, within MMP_RS04470 at 851.239 kb on + strand at 851.240 kb on - strand at 851.241 kb on + strand at 851.260 kb on - strand at 851.261 kb on + strand at 851.264 kb on - strand at 851.264 kb on - strand at 851.264 kb on - strand at 851.267 kb on - strand at 851.268 kb on + strand at 851.268 kb on + strand at 851.269 kb on - strand at 851.269 kb on - strand at 851.276 kb on - strand at 851.293 kb on + strand at 851.382 kb on + strand, within MMP_RS04475 at 851.382 kb on + strand, within MMP_RS04475 at 851.444 kb on - strand, within MMP_RS04475 at 851.484 kb on - strand, within MMP_RS04475 at 851.486 kb on - strand, within MMP_RS04475 at 851.513 kb on + strand, within MMP_RS04475 at 851.934 kb on - strand at 851.944 kb on - strand at 851.945 kb on + strand at 851.957 kb on + strand at 851.966 kb on - strand at 851.971 kb on - strand at 851.972 kb on + strand at 851.975 kb on - strand at 851.976 kb on + strand at 851.976 kb on + strand at 851.976 kb on + strand at 851.998 kb on - strand at 852.002 kb on - strand at 852.041 kb on - strand at 852.046 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.054 kb on + strand at 852.056 kb on - strand at 852.057 kb on + strand at 852.057 kb on + strand at 852.074 kb on + strand at 852.102 kb on - strand at 852.102 kb on - strand at 852.103 kb on + strand at 852.105 kb on + strand at 852.179 kb on - strand at 852.180 kb on + strand at 852.247 kb on - strand at 852.248 kb on + strand at 852.260 kb on - strand at 852.265 kb on - strand at 852.319 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction N2 fixation, H2 media remove 849,013 + MMP_RS04465 0.33 -4.3 849,270 + MMP_RS04465 0.51 -4.5 849,361 + MMP_RS04465 0.57 -5.6 849,363 + MMP_RS04465 0.57 -4.1 849,388 - MMP_RS04465 0.59 -5.8 849,463 + MMP_RS04465 0.64 -5.0 849,555 - MMP_RS04465 0.70 -3.9 849,594 - MMP_RS04465 0.73 -5.9 849,595 + MMP_RS04465 0.73 -4.9 849,609 + MMP_RS04465 0.74 -5.7 849,610 - MMP_RS04465 0.74 -4.7 849,610 - MMP_RS04465 0.74 -4.5 849,642 + MMP_RS04465 0.76 -3.8 849,673 + MMP_RS04465 0.79 -5.0 849,746 - MMP_RS04465 0.84 -4.8 849,746 - MMP_RS04465 0.84 -5.5 849,747 + MMP_RS04465 0.84 -5.3 849,747 + MMP_RS04465 0.84 -3.9 849,779 - MMP_RS04465 0.86 -3.5 849,779 - MMP_RS04465 0.86 -4.7 849,780 + MMP_RS04465 0.86 -4.1 849,810 + MMP_RS04465 0.88 -4.2 849,810 + MMP_RS04465 0.88 -4.2 849,810 + MMP_RS04465 0.88 -3.6 849,812 - MMP_RS04465 0.88 -4.1 849,812 - MMP_RS04465 0.88 -5.3 849,935 - -4.8 849,935 - -5.0 849,935 - -4.9 849,936 + -4.5 849,996 + -4.4 849,999 + -4.7 850,061 + -4.8 850,126 + -3.3 850,126 + -4.4 850,204 - MMP_RS04470 0.15 -4.0 850,207 - MMP_RS04470 0.15 -5.5 850,207 - MMP_RS04470 0.15 -5.1 850,270 - MMP_RS04470 0.20 -4.7 850,283 - MMP_RS04470 0.21 -6.1 850,284 + MMP_RS04470 0.21 -6.1 850,284 + MMP_RS04470 0.21 -4.4 850,360 - MMP_RS04470 0.26 -5.3 850,394 - MMP_RS04470 0.29 -4.5 850,395 + MMP_RS04470 0.29 -3.9 850,395 + MMP_RS04470 0.29 -5.2 850,395 + MMP_RS04470 0.29 -4.6 850,400 - MMP_RS04470 0.29 -5.4 850,401 + MMP_RS04470 0.29 -4.2 850,408 - MMP_RS04470 0.30 -5.0 850,408 - MMP_RS04470 0.30 -5.1 850,408 - MMP_RS04470 0.30 -4.0 850,409 + MMP_RS04470 0.30 -4.0 850,409 + MMP_RS04470 0.30 -4.7 850,480 - MMP_RS04470 0.35 -4.7 850,481 + MMP_RS04470 0.35 -1.3 850,553 + MMP_RS04470 0.40 -4.6 850,562 + MMP_RS04470 0.41 -4.3 850,586 - MMP_RS04470 0.43 -5.3 850,587 + MMP_RS04470 0.43 -4.0 850,587 + MMP_RS04470 0.43 -2.7 850,637 - MMP_RS04470 0.46 -5.0 850,805 - MMP_RS04470 0.59 -3.5 850,838 + MMP_RS04470 0.61 -6.4 850,841 - MMP_RS04470 0.61 -4.6 850,841 - MMP_RS04470 0.61 -4.8 850,934 + MMP_RS04470 0.68 -4.1 851,032 - MMP_RS04470 0.75 -4.2 851,037 + MMP_RS04470 0.75 -5.0 851,052 + MMP_RS04470 0.76 -3.7 851,066 + MMP_RS04470 0.77 -5.2 851,126 + MMP_RS04470 0.82 -5.0 851,180 - MMP_RS04470 0.86 -5.9 851,199 - MMP_RS04470 0.87 -4.9 851,205 + MMP_RS04470 0.88 -4.0 851,239 + -3.0 851,240 - -5.0 851,241 + -5.8 851,260 - -4.1 851,261 + -4.5 851,264 - -3.9 851,264 - -5.2 851,264 - -5.4 851,267 - -7.3 851,268 + -5.1 851,268 + -4.1 851,269 - -3.9 851,269 - -4.8 851,276 - -1.6 851,293 + -1.3 851,382 + MMP_RS04475 0.13 -1.3 851,382 + MMP_RS04475 0.13 -0.2 851,444 - MMP_RS04475 0.32 -0.7 851,484 - MMP_RS04475 0.45 +0.0 851,486 - MMP_RS04475 0.45 -0.4 851,513 + MMP_RS04475 0.54 -0.1 851,934 - +0.0 851,944 - +0.2 851,945 + +0.9 851,957 + -0.1 851,966 - -0.8 851,971 - -0.4 851,972 + +0.1 851,975 - -0.9 851,976 + -1.0 851,976 + -0.6 851,976 + -0.4 851,998 - +0.3 852,002 - +0.3 852,041 - -0.5 852,046 - +0.3 852,051 - +0.6 852,051 - -0.1 852,051 - -1.2 852,051 - +0.1 852,054 + -0.0 852,056 - -0.0 852,057 + +0.7 852,057 + +0.1 852,074 + +0.3 852,102 - +0.4 852,102 - -0.1 852,103 + -1.3 852,105 + +0.2 852,179 - -0.7 852,180 + +0.1 852,247 - +0.1 852,248 + +0.2 852,260 - +0.4 852,265 - -1.3 852,319 + -1.0
Or see this region's nucleotide sequence