Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=1hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_14760 and IAI47_14765 are separated by 19 nucleotides IAI47_14765 and IAI47_14770 are separated by 90 nucleotides IAI47_14770 and IAI47_14775 are separated by 3 nucleotides IAI47_14775 and IAI47_14780 are separated by 186 nucleotides
IAI47_14760: IAI47_14760 - transcription antitermination factor NusB, at 3,114,077 to 3,114,496
_14760
IAI47_14765: IAI47_14765 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,114,516 to 3,114,986
_14765
IAI47_14770: IAI47_14770 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 3,115,077 to 3,116,177
_14770
IAI47_14775: IAI47_14775 - transcriptional regulator NrdR, at 3,116,181 to 3,116,630
_14775
IAI47_14780: IAI47_14780 - peptidoglycan-binding protein LysM, at 3,116,817 to 3,117,254
_14780
Position (kb)
3115
3116
3117 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3114.079 kb on - strand at 3115.036 kb on - strand at 3115.036 kb on - strand at 3115.036 kb on - strand at 3115.036 kb on - strand at 3115.041 kb on - strand at 3115.041 kb on - strand at 3115.078 kb on + strand at 3115.079 kb on - strand at 3116.259 kb on - strand, within IAI47_14775 at 3116.261 kb on - strand, within IAI47_14775 at 3116.261 kb on - strand, within IAI47_14775 at 3116.261 kb on - strand, within IAI47_14775 at 3116.261 kb on - strand, within IAI47_14775 at 3116.261 kb on - strand, within IAI47_14775 at 3116.261 kb on - strand, within IAI47_14775 at 3116.271 kb on - strand, within IAI47_14775 at 3116.316 kb on - strand, within IAI47_14775 at 3116.361 kb on - strand, within IAI47_14775 at 3116.447 kb on + strand, within IAI47_14775 at 3116.448 kb on - strand, within IAI47_14775 at 3116.740 kb on + strand at 3116.767 kb on - strand at 3116.767 kb on - strand at 3116.774 kb on - strand at 3116.816 kb on + strand at 3116.816 kb on + strand at 3116.816 kb on + strand at 3116.816 kb on + strand at 3116.816 kb on + strand at 3116.817 kb on - strand at 3116.817 kb on - strand at 3116.817 kb on - strand at 3116.817 kb on - strand at 3116.817 kb on - strand at 3116.891 kb on + strand, within IAI47_14780 at 3116.892 kb on - strand, within IAI47_14780 at 3116.912 kb on + strand, within IAI47_14780 at 3117.032 kb on + strand, within IAI47_14780 at 3117.032 kb on + strand, within IAI47_14780 at 3117.032 kb on + strand, within IAI47_14780 at 3117.033 kb on - strand, within IAI47_14780 at 3117.033 kb on - strand, within IAI47_14780 at 3117.080 kb on + strand, within IAI47_14780 at 3117.081 kb on - strand, within IAI47_14780 at 3117.081 kb on - strand, within IAI47_14780 at 3117.102 kb on + strand, within IAI47_14780 at 3117.103 kb on - strand, within IAI47_14780 at 3117.103 kb on - strand, within IAI47_14780 at 3117.122 kb on + strand, within IAI47_14780 at 3117.150 kb on + strand, within IAI47_14780 at 3117.150 kb on + strand, within IAI47_14780 at 3117.168 kb on + strand, within IAI47_14780 at 3117.168 kb on + strand, within IAI47_14780 at 3117.169 kb on - strand, within IAI47_14780 at 3117.169 kb on - strand, within IAI47_14780 at 3117.169 kb on - strand, within IAI47_14780 at 3117.170 kb on + strand, within IAI47_14780 at 3117.170 kb on + strand, within IAI47_14780 at 3117.170 kb on + strand, within IAI47_14780 at 3117.170 kb on + strand, within IAI47_14780 at 3117.171 kb on - strand, within IAI47_14780
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=1hrs; Outgrowth=LBkan50 remove 3,114,079 - +1.3 3,115,036 - +0.3 3,115,036 - -0.5 3,115,036 - -0.5 3,115,036 - +0.9 3,115,041 - -1.1 3,115,041 - +0.1 3,115,078 + -1.5 3,115,079 - +1.3 3,116,259 - IAI47_14775 0.17 -0.2 3,116,261 - IAI47_14775 0.18 +1.9 3,116,261 - IAI47_14775 0.18 -0.2 3,116,261 - IAI47_14775 0.18 -1.3 3,116,261 - IAI47_14775 0.18 -1.4 3,116,261 - IAI47_14775 0.18 -1.4 3,116,261 - IAI47_14775 0.18 +0.4 3,116,271 - IAI47_14775 0.20 +0.2 3,116,316 - IAI47_14775 0.30 -0.5 3,116,361 - IAI47_14775 0.40 -1.7 3,116,447 + IAI47_14775 0.59 +0.8 3,116,448 - IAI47_14775 0.59 -1.1 3,116,740 + +1.7 3,116,767 - -1.0 3,116,767 - +1.0 3,116,774 - -1.1 3,116,816 + +1.3 3,116,816 + +0.3 3,116,816 + -2.7 3,116,816 + +0.2 3,116,816 + +0.2 3,116,817 - -2.2 3,116,817 - +1.2 3,116,817 - -0.3 3,116,817 - +0.2 3,116,817 - -0.1 3,116,891 + IAI47_14780 0.17 -0.5 3,116,892 - IAI47_14780 0.17 -1.3 3,116,912 + IAI47_14780 0.22 -0.5 3,117,032 + IAI47_14780 0.49 -1.1 3,117,032 + IAI47_14780 0.49 -0.9 3,117,032 + IAI47_14780 0.49 +0.5 3,117,033 - IAI47_14780 0.49 +0.7 3,117,033 - IAI47_14780 0.49 -0.3 3,117,080 + IAI47_14780 0.60 -0.2 3,117,081 - IAI47_14780 0.60 -1.3 3,117,081 - IAI47_14780 0.60 -1.1 3,117,102 + IAI47_14780 0.65 +0.1 3,117,103 - IAI47_14780 0.65 -1.5 3,117,103 - IAI47_14780 0.65 -0.9 3,117,122 + IAI47_14780 0.70 -0.4 3,117,150 + IAI47_14780 0.76 -2.2 3,117,150 + IAI47_14780 0.76 -2.4 3,117,168 + IAI47_14780 0.80 +4.7 3,117,168 + IAI47_14780 0.80 -0.6 3,117,169 - IAI47_14780 0.80 -0.5 3,117,169 - IAI47_14780 0.80 +0.2 3,117,169 - IAI47_14780 0.80 -0.2 3,117,170 + IAI47_14780 0.81 -1.0 3,117,170 + IAI47_14780 0.81 +4.8 3,117,170 + IAI47_14780 0.81 +0.1 3,117,170 + IAI47_14780 0.81 +0.6 3,117,171 - IAI47_14780 0.81 -0.3
Or see this region's nucleotide sequence