Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17325

Experiment: perchlorate 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17315 and BWI76_RS17320 overlap by 4 nucleotidesBWI76_RS17320 and BWI76_RS17325 overlap by 1 nucleotidesBWI76_RS17325 and BWI76_RS17330 overlap by 4 nucleotides BWI76_RS17315: BWI76_RS17315 - EamA family transporter, at 3,584,500 to 3,585,395 _RS17315 BWI76_RS17320: BWI76_RS17320 - respiratory nitrate reductase subunit gamma, at 3,585,392 to 3,586,069 _RS17320 BWI76_RS17325: BWI76_RS17325 - nitrate reductase molybdenum cofactor assembly chaperone, at 3,586,069 to 3,586,779 _RS17325 BWI76_RS17330: BWI76_RS17330 - nitrate reductase subunit beta, at 3,586,776 to 3,588,311 _RS17330 Position (kb) 3586 3587Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7at 3585.328 kb on + strandat 3585.634 kb on + strand, within BWI76_RS17320at 3585.852 kb on + strand, within BWI76_RS17320at 3585.976 kb on - strand, within BWI76_RS17320at 3586.413 kb on + strand, within BWI76_RS17325at 3586.423 kb on + strand, within BWI76_RS17325at 3586.513 kb on - strand, within BWI76_RS17325at 3586.573 kb on + strand, within BWI76_RS17325at 3586.661 kb on - strand, within BWI76_RS17325at 3587.002 kb on + strand, within BWI76_RS17330at 3587.251 kb on + strand, within BWI76_RS17330at 3587.444 kb on - strand, within BWI76_RS17330at 3587.501 kb on + strand, within BWI76_RS17330

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Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 100 mM
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3,585,328 + +0.2
3,585,634 + BWI76_RS17320 0.36 +5.9
3,585,852 + BWI76_RS17320 0.68 +6.1
3,585,976 - BWI76_RS17320 0.86 +6.0
3,586,413 + BWI76_RS17325 0.48 +6.4
3,586,423 + BWI76_RS17325 0.50 +5.9
3,586,513 - BWI76_RS17325 0.62 +6.1
3,586,573 + BWI76_RS17325 0.71 +7.0
3,586,661 - BWI76_RS17325 0.83 +6.1
3,587,002 + BWI76_RS17330 0.15 +6.1
3,587,251 + BWI76_RS17330 0.31 +6.7
3,587,444 - BWI76_RS17330 0.43 +7.1
3,587,501 + BWI76_RS17330 0.47 +6.7

Or see this region's nucleotide sequence