Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05450

Experiment: perchlorate 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05440 and BWI76_RS05445 are separated by 32 nucleotidesBWI76_RS05445 and BWI76_RS05450 overlap by 4 nucleotidesBWI76_RS05450 and BWI76_RS05455 are separated by 65 nucleotidesBWI76_RS05455 and BWI76_RS05460 are separated by 132 nucleotides BWI76_RS05440: BWI76_RS05440 - hydroxyacylglutathione hydrolase, at 1,142,455 to 1,143,210 _RS05440 BWI76_RS05445: BWI76_RS05445 - type 11 methyltransferase, at 1,143,243 to 1,143,965 _RS05445 BWI76_RS05450: BWI76_RS05450 - ribonuclease HI, at 1,143,962 to 1,144,429 _RS05450 BWI76_RS05455: BWI76_RS05455 - DNA polymerase III subunit epsilon, at 1,144,495 to 1,145,226 _RS05455 BWI76_RS05460: BWI76_RS05460 - tRNA-Asp, at 1,145,359 to 1,145,435 _RS05460 Position (kb) 1143 1144 1145Strain fitness (log2 ratio) -2 -1 0 1 2at 1143.029 kb on + strand, within BWI76_RS05440at 1143.076 kb on - strand, within BWI76_RS05440at 1143.264 kb on - strandat 1143.294 kb on + strandat 1143.352 kb on - strand, within BWI76_RS05445at 1143.352 kb on - strand, within BWI76_RS05445at 1143.470 kb on + strand, within BWI76_RS05445at 1143.470 kb on + strand, within BWI76_RS05445at 1143.478 kb on - strand, within BWI76_RS05445at 1143.478 kb on - strand, within BWI76_RS05445at 1143.478 kb on - strand, within BWI76_RS05445at 1143.478 kb on - strand, within BWI76_RS05445at 1143.622 kb on + strand, within BWI76_RS05445at 1143.641 kb on + strand, within BWI76_RS05445at 1144.210 kb on + strand, within BWI76_RS05450at 1144.317 kb on - strand, within BWI76_RS05450at 1144.492 kb on + strandat 1145.308 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction perchlorate 100 mM
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1,143,029 + BWI76_RS05440 0.76 -1.3
1,143,076 - BWI76_RS05440 0.82 +1.0
1,143,264 - -0.1
1,143,294 + +0.2
1,143,352 - BWI76_RS05445 0.15 -0.1
1,143,352 - BWI76_RS05445 0.15 -0.7
1,143,470 + BWI76_RS05445 0.31 -0.2
1,143,470 + BWI76_RS05445 0.31 +0.3
1,143,478 - BWI76_RS05445 0.33 +1.1
1,143,478 - BWI76_RS05445 0.33 -0.0
1,143,478 - BWI76_RS05445 0.33 -0.2
1,143,478 - BWI76_RS05445 0.33 +1.8
1,143,622 + BWI76_RS05445 0.52 +0.8
1,143,641 + BWI76_RS05445 0.55 +1.4
1,144,210 + BWI76_RS05450 0.53 -2.4
1,144,317 - BWI76_RS05450 0.76 -2.0
1,144,492 + +0.1
1,145,308 + +0.2

Or see this region's nucleotide sequence