Experiment: Sebacic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05103 and H281DRAFT_05104 are separated by 94 nucleotides H281DRAFT_05104 and H281DRAFT_05105 are separated by 177 nucleotides H281DRAFT_05105 and H281DRAFT_05106 are separated by 108 nucleotides
H281DRAFT_05103: H281DRAFT_05103 - NusA antitermination factor, at 46,878 to 48,353
_05103
H281DRAFT_05104: H281DRAFT_05104 - bacterial translation initiation factor 2 (bIF-2), at 48,448 to 51,393
_05104
H281DRAFT_05105: H281DRAFT_05105 - ribosome-binding factor A, at 51,571 to 51,936
_05105
H281DRAFT_05106: H281DRAFT_05106 - tRNA pseudouridine synthase B, at 52,045 to 52,980
_05106
Position (kb) 48
49
50
51
52 Strain fitness (log2 ratio) -1
0
1
2 at 48.268 kb on + strand at 48.268 kb on + strand at 52.007 kb on + strand at 52.007 kb on + strand at 52.007 kb on + strand at 52.008 kb on - strand at 52.008 kb on - strand at 52.026 kb on + strand at 52.027 kb on - strand at 52.159 kb on + strand, within H281DRAFT_05106 at 52.159 kb on + strand, within H281DRAFT_05106 at 52.160 kb on - strand, within H281DRAFT_05106 at 52.160 kb on - strand, within H281DRAFT_05106 at 52.160 kb on - strand, within H281DRAFT_05106 at 52.182 kb on + strand, within H281DRAFT_05106 at 52.182 kb on + strand, within H281DRAFT_05106 at 52.182 kb on + strand, within H281DRAFT_05106 at 52.183 kb on - strand, within H281DRAFT_05106
Per-strain Table
Position Strand Gene LocusTag Fraction Sebacic (C) remove 48,268 + -1.2 48,268 + -1.7 52,007 + +0.3 52,007 + -1.5 52,007 + +1.2 52,008 - -1.3 52,008 - +0.3 52,026 + -0.3 52,027 - -1.6 52,159 + H281DRAFT_05106 0.12 -0.8 52,159 + H281DRAFT_05106 0.12 -1.0 52,160 - H281DRAFT_05106 0.12 -0.0 52,160 - H281DRAFT_05106 0.12 -1.2 52,160 - H281DRAFT_05106 0.12 -0.6 52,182 + H281DRAFT_05106 0.15 -0.7 52,182 + H281DRAFT_05106 0.15 -0.2 52,182 + H281DRAFT_05106 0.15 +2.3 52,183 - H281DRAFT_05106 0.15 +0.7
Or see this region's nucleotide sequence