Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3159 and DvMF_3160 are separated by 339 nucleotides DvMF_3160 and DvMF_3161 are separated by 4 nucleotides DvMF_3161 and DvMF_3162 are separated by 138 nucleotides
DvMF_3159: DvMF_3159 - protein of unknown function DUF610 YibQ (RefSeq), at 3,984,270 to 3,985,901
_3159
DvMF_3160: DvMF_3160 - Nucleoside-diphosphate kinase (RefSeq), at 3,986,241 to 3,986,660
_3160
DvMF_3161: DvMF_3161 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 3,986,665 to 3,987,474
_3161
DvMF_3162: DvMF_3162 - Smr protein/MutS2 (RefSeq), at 3,987,613 to 3,988,965
_3162
Position (kb)
3986
3987 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 3985.464 kb on - strand, within DvMF_3159 at 3985.492 kb on - strand, within DvMF_3159 at 3985.831 kb on + strand at 3985.969 kb on + strand at 3985.971 kb on + strand at 3986.149 kb on + strand at 3986.170 kb on - strand at 3986.175 kb on - strand at 3986.934 kb on + strand, within DvMF_3161 at 3987.042 kb on + strand, within DvMF_3161 at 3987.050 kb on - strand, within DvMF_3161 at 3987.191 kb on + strand, within DvMF_3161 at 3987.276 kb on + strand, within DvMF_3161
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 3,985,464 - DvMF_3159 0.73 -0.1 3,985,492 - DvMF_3159 0.75 -0.9 3,985,831 + -0.4 3,985,969 + +0.2 3,985,971 + -0.5 3,986,149 + +1.1 3,986,170 - -0.2 3,986,175 - -0.6 3,986,934 + DvMF_3161 0.33 -7.7 3,987,042 + DvMF_3161 0.47 -3.6 3,987,050 - DvMF_3161 0.48 -6.7 3,987,191 + DvMF_3161 0.65 -3.5 3,987,276 + DvMF_3161 0.75 -5.4
Or see this region's nucleotide sequence