Experiment: Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.2088.1 and Xcc-8004.2089.1 are separated by 64 nucleotides Xcc-8004.2089.1 and Xcc-8004.2091.1 are separated by 40 nucleotides Xcc-8004.2091.1 and Xcc-8004.2092.1 are separated by 7 nucleotides
Xcc-8004.2088.1: Xcc-8004.2088.1 - Xanthan biosynthesis oligosaccharidyl-lipid flippase GumJ, at 2,003,326 to 2,004,825
Xcc-8004.2088.1
Xcc-8004.2089.1: Xcc-8004.2089.1 - Xanthan biosynthesis glucuronosyltransferase GumK, at 2,004,890 to 2,006,092
Xcc-8004.2089.1
Xcc-8004.2091.1: Xcc-8004.2091.1 - Xanthan biosynthesis pyruvyltransferase GumL, at 2,006,133 to 2,006,927
Xcc-8004.2091.1
Xcc-8004.2092.1: Xcc-8004.2092.1 - Xanthan biosynthesis glycosyltransferase GumM, at 2,006,935 to 2,007,726
Xcc-8004.2092.1
Position (kb)
2004
2005
2006
2007 Strain fitness (log2 ratio)
-2
-1
0
1 at 2004.890 kb on - strand at 2004.890 kb on - strand at 2004.938 kb on + strand at 2004.939 kb on - strand at 2005.075 kb on + strand, within Xcc-8004.2089.1 at 2005.075 kb on + strand, within Xcc-8004.2089.1 at 2005.076 kb on - strand, within Xcc-8004.2089.1 at 2005.076 kb on - strand, within Xcc-8004.2089.1 at 2005.218 kb on - strand, within Xcc-8004.2089.1 at 2005.346 kb on + strand, within Xcc-8004.2089.1 at 2005.388 kb on + strand, within Xcc-8004.2089.1 at 2005.389 kb on - strand, within Xcc-8004.2089.1 at 2005.389 kb on - strand, within Xcc-8004.2089.1 at 2005.495 kb on - strand, within Xcc-8004.2089.1 at 2005.697 kb on + strand, within Xcc-8004.2089.1 at 2005.698 kb on - strand, within Xcc-8004.2089.1 at 2005.733 kb on - strand, within Xcc-8004.2089.1 at 2005.890 kb on - strand, within Xcc-8004.2089.1 at 2005.953 kb on + strand, within Xcc-8004.2089.1 at 2005.954 kb on - strand, within Xcc-8004.2089.1 at 2005.954 kb on - strand, within Xcc-8004.2089.1 at 2005.954 kb on - strand, within Xcc-8004.2089.1 at 2006.403 kb on - strand, within Xcc-8004.2091.1 at 2006.422 kb on + strand, within Xcc-8004.2091.1 at 2006.602 kb on - strand, within Xcc-8004.2091.1 at 2006.602 kb on - strand, within Xcc-8004.2091.1 at 2006.724 kb on + strand, within Xcc-8004.2091.1 at 2006.724 kb on + strand, within Xcc-8004.2091.1 at 2006.725 kb on - strand, within Xcc-8004.2091.1 at 2006.742 kb on + strand, within Xcc-8004.2091.1 at 2006.743 kb on - strand, within Xcc-8004.2091.1 at 2006.779 kb on - strand, within Xcc-8004.2091.1 at 2006.787 kb on + strand, within Xcc-8004.2091.1 at 2006.787 kb on + strand, within Xcc-8004.2091.1 at 2006.788 kb on - strand, within Xcc-8004.2091.1 at 2006.788 kb on - strand, within Xcc-8004.2091.1 at 2006.790 kb on + strand, within Xcc-8004.2091.1 at 2006.791 kb on - strand, within Xcc-8004.2091.1
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X remove 2,004,890 - -1.4 2,004,890 - +0.5 2,004,938 + +0.2 2,004,939 - -0.0 2,005,075 + Xcc-8004.2089.1 0.15 -1.2 2,005,075 + Xcc-8004.2089.1 0.15 +1.0 2,005,076 - Xcc-8004.2089.1 0.15 -0.3 2,005,076 - Xcc-8004.2089.1 0.15 -1.1 2,005,218 - Xcc-8004.2089.1 0.27 -1.2 2,005,346 + Xcc-8004.2089.1 0.38 -1.8 2,005,388 + Xcc-8004.2089.1 0.41 -0.2 2,005,389 - Xcc-8004.2089.1 0.41 +0.2 2,005,389 - Xcc-8004.2089.1 0.41 -2.3 2,005,495 - Xcc-8004.2089.1 0.50 -0.6 2,005,697 + Xcc-8004.2089.1 0.67 +0.5 2,005,698 - Xcc-8004.2089.1 0.67 -1.0 2,005,733 - Xcc-8004.2089.1 0.70 -2.3 2,005,890 - Xcc-8004.2089.1 0.83 -0.4 2,005,953 + Xcc-8004.2089.1 0.88 -0.1 2,005,954 - Xcc-8004.2089.1 0.88 -0.3 2,005,954 - Xcc-8004.2089.1 0.88 -0.5 2,005,954 - Xcc-8004.2089.1 0.88 -0.1 2,006,403 - Xcc-8004.2091.1 0.34 -0.8 2,006,422 + Xcc-8004.2091.1 0.36 +0.9 2,006,602 - Xcc-8004.2091.1 0.59 -1.1 2,006,602 - Xcc-8004.2091.1 0.59 -0.1 2,006,724 + Xcc-8004.2091.1 0.74 -0.6 2,006,724 + Xcc-8004.2091.1 0.74 -0.8 2,006,725 - Xcc-8004.2091.1 0.74 +0.7 2,006,742 + Xcc-8004.2091.1 0.77 +0.4 2,006,743 - Xcc-8004.2091.1 0.77 -0.2 2,006,779 - Xcc-8004.2091.1 0.81 +0.5 2,006,787 + Xcc-8004.2091.1 0.82 +0.4 2,006,787 + Xcc-8004.2091.1 0.82 +0.5 2,006,788 - Xcc-8004.2091.1 0.82 -1.1 2,006,788 - Xcc-8004.2091.1 0.82 -0.9 2,006,790 + Xcc-8004.2091.1 0.83 -0.8 2,006,791 - Xcc-8004.2091.1 0.83 -0.3
Or see this region's nucleotide sequence