Experiment: L-Glutamine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c24830 and PGA1_c24840 are separated by 5 nucleotides PGA1_c24840 and PGA1_c24850 are separated by 178 nucleotides PGA1_c24850 and PGA1_c24860 are separated by 129 nucleotides PGA1_c24860 and PGA1_c24870 overlap by 8 nucleotides
PGA1_c24830: PGA1_c24830 - Protein of unknown function (DUF1150)., at 2,589,107 to 2,589,331
_c24830
PGA1_c24840: PGA1_c24840 - small heat shock protein IbpA, at 2,589,337 to 2,589,750
_c24840
PGA1_c24850: PGA1_c24850 - Uncharacterized conserved small protein containing a coiled-coil domain, at 2,589,929 to 2,590,153
_c24850
PGA1_c24860: PGA1_c24860 - phosphoribosylaminoimidazole carboxylase catalytic subunit PurE, at 2,590,283 to 2,590,783
_c24860
PGA1_c24870: PGA1_c24870 - phosphoribosylaminoimidazole carboxylase ATPase subunit PurK, at 2,590,776 to 2,591,864
_c24870
Position (kb)
2590
2591 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2589.289 kb on + strand, within PGA1_c24830 at 2589.297 kb on - strand, within PGA1_c24830 at 2589.306 kb on + strand, within PGA1_c24830 at 2589.314 kb on - strand at 2589.322 kb on - strand at 2589.324 kb on + strand at 2589.324 kb on + strand at 2589.324 kb on - strand at 2589.329 kb on - strand at 2589.332 kb on - strand at 2589.334 kb on + strand at 2589.334 kb on + strand at 2589.342 kb on - strand at 2589.342 kb on - strand at 2589.435 kb on + strand, within PGA1_c24840 at 2589.555 kb on + strand, within PGA1_c24840 at 2589.555 kb on + strand, within PGA1_c24840 at 2589.563 kb on - strand, within PGA1_c24840 at 2589.563 kb on - strand, within PGA1_c24840 at 2589.563 kb on - strand, within PGA1_c24840 at 2589.563 kb on - strand, within PGA1_c24840 at 2589.568 kb on + strand, within PGA1_c24840 at 2589.576 kb on - strand, within PGA1_c24840 at 2589.593 kb on + strand, within PGA1_c24840 at 2589.649 kb on + strand, within PGA1_c24840 at 2589.658 kb on - strand, within PGA1_c24840 at 2589.844 kb on + strand at 2589.845 kb on + strand at 2589.845 kb on + strand at 2589.845 kb on + strand at 2589.845 kb on + strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.853 kb on - strand at 2589.855 kb on + strand at 2589.858 kb on - strand at 2589.860 kb on - strand at 2589.863 kb on - strand at 2589.900 kb on + strand at 2589.900 kb on + strand at 2589.900 kb on + strand at 2589.908 kb on - strand at 2589.908 kb on - strand at 2589.908 kb on - strand at 2589.968 kb on + strand, within PGA1_c24850 at 2589.976 kb on - strand, within PGA1_c24850 at 2590.034 kb on + strand, within PGA1_c24850 at 2590.101 kb on - strand, within PGA1_c24850 at 2590.102 kb on + strand, within PGA1_c24850 at 2590.160 kb on - strand at 2590.162 kb on + strand at 2590.214 kb on - strand at 2590.559 kb on + strand, within PGA1_c24860 at 2590.567 kb on - strand, within PGA1_c24860 at 2591.016 kb on - strand, within PGA1_c24870 at 2591.056 kb on + strand, within PGA1_c24870 at 2591.088 kb on - strand, within PGA1_c24870 at 2591.134 kb on + strand, within PGA1_c24870 at 2591.264 kb on - strand, within PGA1_c24870 at 2591.297 kb on - strand, within PGA1_c24870 at 2591.366 kb on + strand, within PGA1_c24870 at 2591.420 kb on - strand, within PGA1_c24870 at 2591.452 kb on - strand, within PGA1_c24870 at 2591.475 kb on + strand, within PGA1_c24870 at 2591.565 kb on + strand, within PGA1_c24870 at 2591.565 kb on + strand, within PGA1_c24870 at 2591.707 kb on + strand, within PGA1_c24870 at 2591.740 kb on + strand, within PGA1_c24870 at 2591.779 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (C) remove 2,589,289 + PGA1_c24830 0.81 +0.0 2,589,297 - PGA1_c24830 0.84 -0.1 2,589,306 + PGA1_c24830 0.88 -0.3 2,589,314 - +0.1 2,589,322 - -0.4 2,589,324 + +0.9 2,589,324 + +0.9 2,589,324 - +0.2 2,589,329 - +1.0 2,589,332 - -0.6 2,589,334 + +0.1 2,589,334 + -0.1 2,589,342 - -1.2 2,589,342 - +0.5 2,589,435 + PGA1_c24840 0.24 +0.9 2,589,555 + PGA1_c24840 0.53 -0.5 2,589,555 + PGA1_c24840 0.53 +0.4 2,589,563 - PGA1_c24840 0.55 -0.3 2,589,563 - PGA1_c24840 0.55 +0.2 2,589,563 - PGA1_c24840 0.55 -0.3 2,589,563 - PGA1_c24840 0.55 +0.3 2,589,568 + PGA1_c24840 0.56 -0.3 2,589,576 - PGA1_c24840 0.58 -0.2 2,589,593 + PGA1_c24840 0.62 -0.1 2,589,649 + PGA1_c24840 0.75 -1.2 2,589,658 - PGA1_c24840 0.78 -0.5 2,589,844 + +0.6 2,589,845 + -0.8 2,589,845 + -0.1 2,589,845 + +0.5 2,589,845 + -0.8 2,589,853 - +0.2 2,589,853 - +0.9 2,589,853 - +0.3 2,589,853 - +0.1 2,589,853 - -0.6 2,589,853 - +0.1 2,589,853 - +0.5 2,589,853 - -0.9 2,589,853 - +0.8 2,589,853 - -0.2 2,589,853 - -0.8 2,589,853 - +0.2 2,589,853 - +0.1 2,589,853 - +0.2 2,589,853 - -0.1 2,589,855 + -1.1 2,589,858 - -0.4 2,589,860 - +0.8 2,589,863 - +0.2 2,589,900 + +0.0 2,589,900 + -1.4 2,589,900 + -0.6 2,589,908 - +0.1 2,589,908 - -0.3 2,589,908 - +0.2 2,589,968 + PGA1_c24850 0.17 -0.1 2,589,976 - PGA1_c24850 0.21 +0.8 2,590,034 + PGA1_c24850 0.47 -0.2 2,590,101 - PGA1_c24850 0.76 +0.5 2,590,102 + PGA1_c24850 0.77 +0.9 2,590,160 - -0.8 2,590,162 + -0.1 2,590,214 - -2.0 2,590,559 + PGA1_c24860 0.55 -1.5 2,590,567 - PGA1_c24860 0.57 -4.2 2,591,016 - PGA1_c24870 0.22 -2.9 2,591,056 + PGA1_c24870 0.26 -2.1 2,591,088 - PGA1_c24870 0.29 -2.0 2,591,134 + PGA1_c24870 0.33 -2.6 2,591,264 - PGA1_c24870 0.45 -3.8 2,591,297 - PGA1_c24870 0.48 -2.5 2,591,366 + PGA1_c24870 0.54 -1.4 2,591,420 - PGA1_c24870 0.59 -1.4 2,591,452 - PGA1_c24870 0.62 -1.7 2,591,475 + PGA1_c24870 0.64 -3.2 2,591,565 + PGA1_c24870 0.72 -2.3 2,591,565 + PGA1_c24870 0.72 -2.6 2,591,707 + PGA1_c24870 0.85 -3.9 2,591,740 + PGA1_c24870 0.89 -1.9 2,591,779 - -2.1
Or see this region's nucleotide sequence