Experiment: MoYLS4 with Mercury 10uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1674 and DVU1675 are separated by 58 nucleotides DVU1675 and tRNA-Leu are separated by 240 nucleotides tRNA-Leu and secG are separated by 12 nucleotides secG and tpiA are separated by 37 nucleotides
DVU1674: DVU1674 - transcriptional regulator, putative (TIGR), at 1,754,716 to 1,755,330
DVU1674
DVU1675: DVU1675 - hypothetical protein (TIGR), at 1,755,389 to 1,755,553
DVU1675
DVU_tRNA-Leu-4: tRNA-Leu - tRNA with anticodon GAG for Leu (VIMSS-AUTO), at 1,755,794 to 1,755,880
tRNA-Leu
DVU1676: secG - preprotein translocase, SecG subunit (TIGR), at 1,755,893 to 1,756,243
secG
DVU1677: tpiA - triosephosphate isomerase (TIGR), at 1,756,281 to 1,757,036
tpiA
Position (kb) 1755
1756 Strain fitness (log2 ratio) -1
0
1 at 1754.881 kb on + strand, within DVU1674 at 1755.330 kb on - strand at 1755.564 kb on - strand at 1755.670 kb on - strand at 1755.675 kb on + strand at 1755.818 kb on - strand, within tRNA-Leu at 1755.843 kb on + strand, within tRNA-Leu at 1755.924 kb on + strand at 1755.932 kb on - strand, within secG at 1755.999 kb on + strand, within secG
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Mercury 10uM remove 1,754,881 + DVU1674 0.27 +0.7 1,755,330 - -0.3 1,755,564 - -0.2 1,755,670 - +0.9 1,755,675 + -0.7 1,755,818 - tRNA-Leu DVU_tRNA-Leu-4 0.28 -0.3 1,755,843 + tRNA-Leu DVU_tRNA-Leu-4 0.56 +0.5 1,755,924 + -0.4 1,755,932 - secG DVU1676 0.11 -1.6 1,755,999 + secG DVU1676 0.30 +0.0
Or see this region's nucleotide sequence