Experiment: MoYLS4 with Mercury 10uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt oorB and oorC overlap by 4 nucleotides oorC and DVU1948 are separated by 10 nucleotides DVU1948 and nifA-1 are separated by 264 nucleotides
DVU1946: oorB - pyruvate ferredoxin oxidoreductase, beta subunit, putative (TIGR), at 2,019,067 to 2,019,897
oorB
DVU1947: oorC - pyruvate ferredoxin oxidoreductase, gamma subunit, putative (TIGR), at 2,019,894 to 2,020,517
oorC
DVU1948: DVU1948 - conserved hypothetical protein (TIGR), at 2,020,528 to 2,020,818
DVU1948
DVU1949: nifA-1 - nif-specific regulatory protein (TIGR), at 2,021,083 to 2,022,672
nifA-1
Position (kb)
2020
2021 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2019.537 kb on - strand, within oorB at 2019.672 kb on + strand, within oorB at 2019.683 kb on + strand, within oorB at 2019.765 kb on - strand, within oorB at 2019.925 kb on + strand at 2019.949 kb on + strand at 2020.089 kb on - strand, within oorC at 2020.098 kb on - strand, within oorC at 2020.164 kb on + strand, within oorC at 2020.301 kb on + strand, within oorC at 2020.596 kb on + strand, within DVU1948 at 2020.596 kb on + strand, within DVU1948 at 2020.696 kb on + strand, within DVU1948 at 2020.752 kb on + strand, within DVU1948 at 2020.787 kb on + strand, within DVU1948 at 2020.789 kb on + strand, within DVU1948 at 2020.795 kb on - strand at 2020.797 kb on - strand at 2021.063 kb on + strand at 2021.085 kb on + strand at 2021.353 kb on + strand, within nifA-1 at 2021.361 kb on - strand, within nifA-1 at 2021.661 kb on + strand, within nifA-1 at 2021.689 kb on - strand, within nifA-1
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Mercury 10uM remove 2,019,537 - oorB DVU1946 0.57 +0.2 2,019,672 + oorB DVU1946 0.73 -0.6 2,019,683 + oorB DVU1946 0.74 +0.1 2,019,765 - oorB DVU1946 0.84 -0.1 2,019,925 + +0.0 2,019,949 + -1.1 2,020,089 - oorC DVU1947 0.31 +0.2 2,020,098 - oorC DVU1947 0.33 +0.1 2,020,164 + oorC DVU1947 0.43 -0.2 2,020,301 + oorC DVU1947 0.65 +2.8 2,020,596 + DVU1948 0.23 +0.6 2,020,596 + DVU1948 0.23 +0.1 2,020,696 + DVU1948 0.58 +0.5 2,020,752 + DVU1948 0.77 +0.1 2,020,787 + DVU1948 0.89 -0.2 2,020,789 + DVU1948 0.90 +0.0 2,020,795 - -0.4 2,020,797 - -0.5 2,021,063 + -0.1 2,021,085 + -0.8 2,021,353 + nifA-1 DVU1949 0.17 +0.3 2,021,361 - nifA-1 DVU1949 0.17 +0.4 2,021,661 + nifA-1 DVU1949 0.36 -0.0 2,021,689 - nifA-1 DVU1949 0.38 +0.7
Or see this region's nucleotide sequence