Experiment: MoYLS4 with Mercury 10uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pppA and DVU1264 are separated by 307 nucleotides DVU1264 and DVU1265 are separated by 10 nucleotides
DVU1263: pppA - type IV prepilin-like proteins leader peptidase (TIGR), at 1,351,308 to 1,352,111
pppA
DVU1264: DVU1264 - transglycosylase SLT domain protein (TIGR), at 1,352,419 to 1,353,180
DVU1264
DVU1265: DVU1265 - hypothetical protein (TIGR), at 1,353,191 to 1,353,940
DVU1265
Position (kb)
1352
1353
1354 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1351.527 kb on + strand, within pppA at 1351.541 kb on + strand, within pppA at 1351.615 kb on - strand, within pppA at 1351.632 kb on + strand, within pppA at 1351.790 kb on - strand, within pppA at 1351.790 kb on - strand, within pppA at 1351.839 kb on + strand, within pppA at 1351.851 kb on + strand, within pppA at 1351.861 kb on - strand, within pppA at 1351.877 kb on + strand, within pppA at 1352.138 kb on + strand at 1352.253 kb on - strand at 1352.282 kb on - strand at 1352.339 kb on + strand at 1352.342 kb on - strand at 1352.347 kb on - strand at 1352.402 kb on - strand at 1352.404 kb on - strand at 1352.406 kb on - strand at 1352.415 kb on + strand at 1352.496 kb on - strand, within DVU1264 at 1352.577 kb on + strand, within DVU1264 at 1352.605 kb on - strand, within DVU1264 at 1352.617 kb on + strand, within DVU1264 at 1352.642 kb on - strand, within DVU1264 at 1352.702 kb on - strand, within DVU1264 at 1352.745 kb on + strand, within DVU1264 at 1352.796 kb on - strand, within DVU1264 at 1353.004 kb on - strand, within DVU1264 at 1353.065 kb on + strand, within DVU1264 at 1353.075 kb on + strand, within DVU1264 at 1353.078 kb on - strand, within DVU1264 at 1353.083 kb on - strand, within DVU1264 at 1353.083 kb on - strand, within DVU1264 at 1353.109 kb on + strand at 1353.116 kb on - strand at 1353.121 kb on + strand at 1353.121 kb on + strand at 1353.121 kb on + strand at 1353.121 kb on + strand at 1353.121 kb on + strand at 1353.129 kb on - strand at 1353.129 kb on - strand at 1353.129 kb on - strand at 1353.129 kb on - strand at 1353.129 kb on - strand at 1353.139 kb on - strand at 1353.143 kb on + strand at 1353.185 kb on - strand at 1353.231 kb on + strand at 1353.231 kb on + strand at 1353.258 kb on - strand at 1353.284 kb on + strand, within DVU1265 at 1353.292 kb on - strand, within DVU1265 at 1353.341 kb on + strand, within DVU1265 at 1353.529 kb on - strand, within DVU1265 at 1353.662 kb on + strand, within DVU1265 at 1353.678 kb on - strand, within DVU1265 at 1353.678 kb on - strand, within DVU1265 at 1353.768 kb on + strand, within DVU1265 at 1353.851 kb on - strand, within DVU1265 at 1353.974 kb on + strand at 1353.974 kb on + strand at 1353.975 kb on - strand at 1354.061 kb on + strand at 1354.136 kb on - strand at 1354.136 kb on - strand at 1354.161 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Mercury 10uM remove 1,351,527 + pppA DVU1263 0.27 -0.3 1,351,541 + pppA DVU1263 0.29 +0.1 1,351,615 - pppA DVU1263 0.38 +0.4 1,351,632 + pppA DVU1263 0.40 +0.0 1,351,790 - pppA DVU1263 0.60 +2.0 1,351,790 - pppA DVU1263 0.60 +0.4 1,351,839 + pppA DVU1263 0.66 -0.4 1,351,851 + pppA DVU1263 0.68 -0.3 1,351,861 - pppA DVU1263 0.69 +0.9 1,351,877 + pppA DVU1263 0.71 -1.0 1,352,138 + -0.1 1,352,253 - -0.9 1,352,282 - -0.6 1,352,339 + -0.7 1,352,342 - +0.1 1,352,347 - -0.6 1,352,402 - +0.7 1,352,404 - -0.5 1,352,406 - +0.2 1,352,415 + +0.7 1,352,496 - DVU1264 0.10 +0.8 1,352,577 + DVU1264 0.21 +0.1 1,352,605 - DVU1264 0.24 +1.7 1,352,617 + DVU1264 0.26 +1.1 1,352,642 - DVU1264 0.29 +0.1 1,352,702 - DVU1264 0.37 +0.0 1,352,745 + DVU1264 0.43 +0.3 1,352,796 - DVU1264 0.49 -1.6 1,353,004 - DVU1264 0.77 +0.8 1,353,065 + DVU1264 0.85 +0.7 1,353,075 + DVU1264 0.86 -0.6 1,353,078 - DVU1264 0.86 +0.4 1,353,083 - DVU1264 0.87 +0.3 1,353,083 - DVU1264 0.87 +0.5 1,353,109 + -0.7 1,353,116 - +0.2 1,353,121 + -0.0 1,353,121 + +0.4 1,353,121 + +0.2 1,353,121 + +0.2 1,353,121 + +0.6 1,353,129 - -1.4 1,353,129 - -0.5 1,353,129 - -0.2 1,353,129 - -0.3 1,353,129 - +0.5 1,353,139 - -0.2 1,353,143 + +0.2 1,353,185 - +0.0 1,353,231 + -1.9 1,353,231 + +0.2 1,353,258 - -0.5 1,353,284 + DVU1265 0.12 -0.1 1,353,292 - DVU1265 0.13 -0.1 1,353,341 + DVU1265 0.20 +0.2 1,353,529 - DVU1265 0.45 -0.2 1,353,662 + DVU1265 0.63 -0.1 1,353,678 - DVU1265 0.65 +0.1 1,353,678 - DVU1265 0.65 -0.1 1,353,768 + DVU1265 0.77 -0.4 1,353,851 - DVU1265 0.88 +0.5 1,353,974 + -0.2 1,353,974 + -0.0 1,353,975 - +0.4 1,354,061 + -0.8 1,354,136 - +0.2 1,354,136 - -0.3 1,354,161 + +0.3
Or see this region's nucleotide sequence