Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26125

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26115 and BWI76_RS26120 overlap by 4 nucleotidesBWI76_RS26120 and BWI76_RS26125 are separated by 117 nucleotidesBWI76_RS26125 and BWI76_RS26130 are separated by 44 nucleotidesBWI76_RS26130 and BWI76_RS26135 are separated by 331 nucleotides BWI76_RS26115: BWI76_RS26115 - hypothetical protein, at 5,394,094 to 5,394,627 _RS26115 BWI76_RS26120: BWI76_RS26120 - hypothetical protein, at 5,394,624 to 5,395,415 _RS26120 BWI76_RS26125: BWI76_RS26125 - carboxypeptidase/penicillin-binding protein 1A, at 5,395,533 to 5,398,091 _RS26125 BWI76_RS26130: BWI76_RS26130 - ADP compounds hydrolase NudE, at 5,398,136 to 5,398,696 _RS26130 BWI76_RS26135: BWI76_RS26135 - Intracellular growth attenuator protein igaA, at 5,399,028 to 5,401,157 _RS26135 Position (kb) 5395 5396 5397 5398 5399Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5394.622 kb on + strandat 5394.664 kb on - strandat 5394.887 kb on + strand, within BWI76_RS26120at 5395.067 kb on + strand, within BWI76_RS26120at 5395.315 kb on - strand, within BWI76_RS26120at 5395.322 kb on + strand, within BWI76_RS26120at 5395.471 kb on + strandat 5395.580 kb on + strandat 5395.580 kb on + strandat 5395.588 kb on - strandat 5395.636 kb on - strandat 5395.646 kb on + strandat 5395.654 kb on + strandat 5395.734 kb on - strandat 5396.697 kb on + strand, within BWI76_RS26125at 5396.761 kb on - strand, within BWI76_RS26125at 5396.761 kb on - strand, within BWI76_RS26125at 5396.779 kb on - strand, within BWI76_RS26125at 5396.796 kb on - strand, within BWI76_RS26125at 5396.886 kb on + strand, within BWI76_RS26125at 5396.942 kb on + strand, within BWI76_RS26125at 5396.950 kb on - strand, within BWI76_RS26125at 5396.952 kb on + strand, within BWI76_RS26125at 5396.993 kb on - strand, within BWI76_RS26125at 5397.181 kb on + strand, within BWI76_RS26125at 5397.488 kb on + strand, within BWI76_RS26125at 5397.567 kb on + strand, within BWI76_RS26125at 5397.690 kb on + strand, within BWI76_RS26125at 5397.887 kb on + strandat 5398.484 kb on - strand, within BWI76_RS26130at 5398.513 kb on + strand, within BWI76_RS26130at 5398.609 kb on - strand, within BWI76_RS26130at 5398.744 kb on + strandat 5398.891 kb on + strandat 5398.899 kb on - strandat 5398.899 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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5,394,622 + +0.3
5,394,664 - +0.5
5,394,887 + BWI76_RS26120 0.33 +1.2
5,395,067 + BWI76_RS26120 0.56 +0.4
5,395,315 - BWI76_RS26120 0.87 +0.9
5,395,322 + BWI76_RS26120 0.88 -0.6
5,395,471 + +2.2
5,395,580 + -1.0
5,395,580 + -0.6
5,395,588 - -2.4
5,395,636 - -2.2
5,395,646 + -1.2
5,395,654 + -0.7
5,395,734 - -1.8
5,396,697 + BWI76_RS26125 0.45 +1.2
5,396,761 - BWI76_RS26125 0.48 -1.1
5,396,761 - BWI76_RS26125 0.48 -1.7
5,396,779 - BWI76_RS26125 0.49 -1.2
5,396,796 - BWI76_RS26125 0.49 -1.4
5,396,886 + BWI76_RS26125 0.53 -0.4
5,396,942 + BWI76_RS26125 0.55 -0.7
5,396,950 - BWI76_RS26125 0.55 -2.3
5,396,952 + BWI76_RS26125 0.55 -1.7
5,396,993 - BWI76_RS26125 0.57 -1.7
5,397,181 + BWI76_RS26125 0.64 -1.9
5,397,488 + BWI76_RS26125 0.76 -1.3
5,397,567 + BWI76_RS26125 0.79 +1.4
5,397,690 + BWI76_RS26125 0.84 -0.6
5,397,887 + +0.1
5,398,484 - BWI76_RS26130 0.62 -1.7
5,398,513 + BWI76_RS26130 0.67 -2.8
5,398,609 - BWI76_RS26130 0.84 -1.6
5,398,744 + +0.8
5,398,891 + -0.2
5,398,899 - -1.0
5,398,899 - +0.7

Or see this region's nucleotide sequence