Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25475 and BWI76_RS25480 are separated by 78 nucleotides BWI76_RS25480 and BWI76_RS25485 are separated by 2 nucleotides BWI76_RS25485 and BWI76_RS25490 are separated by 7 nucleotides
BWI76_RS25475: BWI76_RS25475 - TIGR02099 family protein, at 5,293,022 to 5,296,819
_RS25475
BWI76_RS25480: BWI76_RS25480 - ribonuclease E/G, at 5,296,898 to 5,298,367
_RS25480
BWI76_RS25485: BWI76_RS25485 - septum formation protein Maf, at 5,298,370 to 5,298,951
_RS25485
BWI76_RS25490: BWI76_RS25490 - rod shape-determining protein MreD, at 5,298,959 to 5,299,447
_RS25490
Position (kb) 5296
5297
5298
5299 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5296.213 kb on + strand, within BWI76_RS25475 at 5296.223 kb on + strand, within BWI76_RS25475 at 5296.386 kb on - strand, within BWI76_RS25475 at 5296.386 kb on - strand, within BWI76_RS25475 at 5296.425 kb on + strand, within BWI76_RS25475 at 5296.860 kb on - strand at 5297.232 kb on - strand, within BWI76_RS25480 at 5297.312 kb on + strand, within BWI76_RS25480 at 5297.525 kb on - strand, within BWI76_RS25480 at 5297.591 kb on - strand, within BWI76_RS25480 at 5297.593 kb on + strand, within BWI76_RS25480 at 5297.654 kb on + strand, within BWI76_RS25480 at 5297.865 kb on + strand, within BWI76_RS25480 at 5298.411 kb on + strand at 5298.870 kb on - strand, within BWI76_RS25485
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,296,213 + BWI76_RS25475 0.84 -1.8 5,296,223 + BWI76_RS25475 0.84 -3.2 5,296,386 - BWI76_RS25475 0.89 -1.7 5,296,386 - BWI76_RS25475 0.89 -0.3 5,296,425 + BWI76_RS25475 0.90 -1.2 5,296,860 - -1.6 5,297,232 - BWI76_RS25480 0.23 -0.8 5,297,312 + BWI76_RS25480 0.28 -0.9 5,297,525 - BWI76_RS25480 0.43 -3.5 5,297,591 - BWI76_RS25480 0.47 -1.7 5,297,593 + BWI76_RS25480 0.47 -0.3 5,297,654 + BWI76_RS25480 0.51 -1.6 5,297,865 + BWI76_RS25480 0.66 -0.8 5,298,411 + -0.3 5,298,870 - BWI76_RS25485 0.86 +0.7
Or see this region's nucleotide sequence