Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25065 and BWI76_RS25070 overlap by 42 nucleotides BWI76_RS25070 and BWI76_RS25075 are separated by 71 nucleotides BWI76_RS25075 and BWI76_RS25080 are separated by 147 nucleotides BWI76_RS25080 and BWI76_RS25085 overlap by 1 nucleotides BWI76_RS25085 and BWI76_RS25090 are separated by 91 nucleotides
BWI76_RS25065: BWI76_RS25065 - polyribonucleotide nucleotidyltransferase, at 5,218,446 to 5,220,584
_RS25065
BWI76_RS25070: BWI76_RS25070 - hypothetical protein, at 5,220,543 to 5,220,754
_RS25070
BWI76_RS25075: BWI76_RS25075 - 30S ribosomal protein S15, at 5,220,826 to 5,221,095
_RS25075
BWI76_RS25080: BWI76_RS25080 - tRNA pseudouridine(55) synthase, at 5,221,243 to 5,222,187
_RS25080
BWI76_RS25085: BWI76_RS25085 - ribosome-binding factor A, at 5,222,187 to 5,222,588
_RS25085
BWI76_RS25090: BWI76_RS25090 - translation initiation factor IF-2, at 5,222,680 to 5,225,370
_RS25090
Position (kb)
5221
5222
5223 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5220.430 kb on + strand at 5220.472 kb on + strand at 5220.681 kb on - strand, within BWI76_RS25070 at 5220.709 kb on - strand at 5221.250 kb on + strand at 5221.407 kb on - strand, within BWI76_RS25080 at 5221.453 kb on + strand, within BWI76_RS25080 at 5221.520 kb on + strand, within BWI76_RS25080 at 5221.746 kb on - strand, within BWI76_RS25080 at 5221.852 kb on + strand, within BWI76_RS25080
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,220,430 + +0.2 5,220,472 + +1.6 5,220,681 - BWI76_RS25070 0.65 -0.5 5,220,709 - +2.6 5,221,250 + +1.0 5,221,407 - BWI76_RS25080 0.17 +3.1 5,221,453 + BWI76_RS25080 0.22 +2.6 5,221,520 + BWI76_RS25080 0.29 +2.3 5,221,746 - BWI76_RS25080 0.53 +1.9 5,221,852 + BWI76_RS25080 0.64 +2.0
Or see this region's nucleotide sequence