Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25050

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25045 and BWI76_RS25050 are separated by 98 nucleotidesBWI76_RS25050 and BWI76_RS25055 are separated by 144 nucleotides BWI76_RS25045: BWI76_RS25045 - Malonate utilization transacting regulator MdcR, at 5,212,890 to 5,213,831 _RS25045 BWI76_RS25050: BWI76_RS25050 - tryptophan permease, at 5,213,930 to 5,215,174 _RS25050 BWI76_RS25055: BWI76_RS25055 - DEAD/DEAH family ATP-dependent RNA helicase, at 5,215,319 to 5,217,280 _RS25055 Position (kb) 5213 5214 5215 5216Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 5212.943 kb on - strandat 5212.965 kb on + strandat 5212.970 kb on - strandat 5212.970 kb on - strandat 5213.026 kb on - strand, within BWI76_RS25045at 5213.040 kb on - strand, within BWI76_RS25045at 5213.051 kb on + strand, within BWI76_RS25045at 5213.059 kb on - strandat 5213.059 kb on - strand, within BWI76_RS25045at 5213.059 kb on - strand, within BWI76_RS25045at 5213.067 kb on - strand, within BWI76_RS25045at 5213.070 kb on - strand, within BWI76_RS25045at 5213.070 kb on - strand, within BWI76_RS25045at 5213.072 kb on - strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.098 kb on + strand, within BWI76_RS25045at 5213.098 kb on + strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.120 kb on - strand, within BWI76_RS25045at 5213.122 kb on + strand, within BWI76_RS25045at 5213.125 kb on - strand, within BWI76_RS25045at 5213.130 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.195 kb on + strand, within BWI76_RS25045at 5213.195 kb on + strand, within BWI76_RS25045at 5213.195 kb on + strand, within BWI76_RS25045at 5213.195 kb on + strand, within BWI76_RS25045at 5213.203 kb on - strand, within BWI76_RS25045at 5213.203 kb on - strand, within BWI76_RS25045at 5213.203 kb on - strand, within BWI76_RS25045at 5213.203 kb on - strand, within BWI76_RS25045at 5213.209 kb on - strand, within BWI76_RS25045at 5213.209 kb on - strand, within BWI76_RS25045at 5213.220 kb on + strand, within BWI76_RS25045at 5213.220 kb on + strand, within BWI76_RS25045at 5213.220 kb on - strand, within BWI76_RS25045at 5213.228 kb on - strand, within BWI76_RS25045at 5213.228 kb on - strand, within BWI76_RS25045at 5213.228 kb on - strand, within BWI76_RS25045at 5213.229 kb on - strand, within BWI76_RS25045at 5213.233 kb on + strand, within BWI76_RS25045at 5213.267 kb on - strand, within BWI76_RS25045at 5213.272 kb on - strand, within BWI76_RS25045at 5213.275 kb on + strand, within BWI76_RS25045at 5213.275 kb on + strand, within BWI76_RS25045at 5213.275 kb on + strand, within BWI76_RS25045at 5213.275 kb on - strand, within BWI76_RS25045at 5213.283 kb on + strand, within BWI76_RS25045at 5213.284 kb on - strand, within BWI76_RS25045at 5213.288 kb on + strand, within BWI76_RS25045at 5213.291 kb on - strand, within BWI76_RS25045at 5213.298 kb on + strand, within BWI76_RS25045at 5213.306 kb on + strandat 5213.306 kb on - strand, within BWI76_RS25045at 5213.306 kb on - strand, within BWI76_RS25045at 5213.314 kb on - strand, within BWI76_RS25045at 5213.314 kb on - strand, within BWI76_RS25045at 5213.372 kb on - strand, within BWI76_RS25045at 5213.374 kb on + strand, within BWI76_RS25045at 5213.459 kb on + strand, within BWI76_RS25045at 5213.459 kb on + strand, within BWI76_RS25045at 5213.467 kb on - strandat 5213.467 kb on - strand, within BWI76_RS25045at 5213.467 kb on - strand, within BWI76_RS25045at 5213.467 kb on - strand, within BWI76_RS25045at 5213.467 kb on - strand, within BWI76_RS25045at 5213.503 kb on + strand, within BWI76_RS25045at 5213.523 kb on + strand, within BWI76_RS25045at 5213.536 kb on + strand, within BWI76_RS25045at 5213.548 kb on + strand, within BWI76_RS25045at 5213.604 kb on - strand, within BWI76_RS25045at 5213.630 kb on + strand, within BWI76_RS25045at 5213.638 kb on - strandat 5213.675 kb on + strand, within BWI76_RS25045at 5213.675 kb on + strand, within BWI76_RS25045at 5213.717 kb on + strand, within BWI76_RS25045at 5213.774 kb on + strandat 5213.777 kb on + strandat 5213.777 kb on + strandat 5213.777 kb on + strandat 5213.782 kb on - strandat 5213.785 kb on - strandat 5213.810 kb on + strandat 5213.942 kb on + strandat 5213.950 kb on + strandat 5213.950 kb on - strandat 5213.958 kb on - strandat 5213.974 kb on - strandat 5213.979 kb on + strandat 5213.984 kb on + strandat 5213.984 kb on + strandat 5213.987 kb on - strandat 5213.987 kb on - strandat 5213.992 kb on - strandat 5213.992 kb on - strandat 5213.992 kb on - strandat 5213.992 kb on - strandat 5213.992 kb on - strandat 5213.997 kb on - strandat 5213.997 kb on - strandat 5214.002 kb on + strandat 5214.004 kb on - strandat 5214.004 kb on - strandat 5214.010 kb on - strandat 5214.010 kb on - strandat 5214.033 kb on + strandat 5214.040 kb on + strandat 5214.058 kb on - strand, within BWI76_RS25050at 5214.176 kb on - strand, within BWI76_RS25050at 5214.189 kb on - strand, within BWI76_RS25050at 5214.280 kb on + strand, within BWI76_RS25050at 5214.333 kb on - strand, within BWI76_RS25050at 5214.349 kb on - strand, within BWI76_RS25050at 5214.423 kb on + strand, within BWI76_RS25050at 5214.590 kb on - strand, within BWI76_RS25050at 5214.627 kb on + strand, within BWI76_RS25050at 5214.784 kb on + strand, within BWI76_RS25050at 5214.822 kb on + strand, within BWI76_RS25050at 5214.830 kb on + strand, within BWI76_RS25050at 5214.895 kb on - strand, within BWI76_RS25050at 5214.908 kb on - strand, within BWI76_RS25050at 5214.918 kb on - strand, within BWI76_RS25050at 5215.093 kb on - strandat 5215.128 kb on + strandat 5215.497 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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5,212,943 - -1.1
5,212,965 + -0.4
5,212,970 - +0.4
5,212,970 - -0.6
5,213,026 - BWI76_RS25045 0.14 +0.3
5,213,040 - BWI76_RS25045 0.16 +1.1
5,213,051 + BWI76_RS25045 0.17 +0.1
5,213,059 - +1.6
5,213,059 - BWI76_RS25045 0.18 -1.0
5,213,059 - BWI76_RS25045 0.18 +0.9
5,213,067 - BWI76_RS25045 0.19 +0.5
5,213,070 - BWI76_RS25045 0.19 -1.6
5,213,070 - BWI76_RS25045 0.19 -1.1
5,213,072 - BWI76_RS25045 0.19 -0.8
5,213,097 + BWI76_RS25045 0.22 +0.6
5,213,097 + BWI76_RS25045 0.22 +5.1
5,213,097 + BWI76_RS25045 0.22 +0.1
5,213,097 + BWI76_RS25045 0.22 -1.9
5,213,097 + BWI76_RS25045 0.22 -1.0
5,213,098 + BWI76_RS25045 0.22 -0.8
5,213,098 + BWI76_RS25045 0.22 +1.6
5,213,105 - BWI76_RS25045 0.23 +0.1
5,213,105 - BWI76_RS25045 0.23 -0.4
5,213,105 - BWI76_RS25045 0.23 +1.0
5,213,105 - BWI76_RS25045 0.23 +1.8
5,213,105 - BWI76_RS25045 0.23 -2.3
5,213,105 - BWI76_RS25045 0.23 -1.8
5,213,105 - BWI76_RS25045 0.23 -1.4
5,213,105 - BWI76_RS25045 0.23 -0.2
5,213,105 - BWI76_RS25045 0.23 -0.1
5,213,105 - BWI76_RS25045 0.23 +0.1
5,213,105 - BWI76_RS25045 0.23 +0.6
5,213,120 - BWI76_RS25045 0.24 -2.2
5,213,122 + BWI76_RS25045 0.25 -0.8
5,213,125 - BWI76_RS25045 0.25 -1.3
5,213,130 - BWI76_RS25045 0.25 +0.0
5,213,164 - BWI76_RS25045 0.29 -0.3
5,213,164 - BWI76_RS25045 0.29 -0.0
5,213,164 - BWI76_RS25045 0.29 +0.2
5,213,164 - BWI76_RS25045 0.29 -0.3
5,213,164 - BWI76_RS25045 0.29 +0.4
5,213,195 + BWI76_RS25045 0.32 -0.2
5,213,195 + BWI76_RS25045 0.32 +0.8
5,213,195 + BWI76_RS25045 0.32 -0.3
5,213,195 + BWI76_RS25045 0.32 -1.8
5,213,203 - BWI76_RS25045 0.33 -0.7
5,213,203 - BWI76_RS25045 0.33 +2.4
5,213,203 - BWI76_RS25045 0.33 -1.1
5,213,203 - BWI76_RS25045 0.33 -1.0
5,213,209 - BWI76_RS25045 0.34 -1.2
5,213,209 - BWI76_RS25045 0.34 -0.0
5,213,220 + BWI76_RS25045 0.35 +1.0
5,213,220 + BWI76_RS25045 0.35 -1.7
5,213,220 - BWI76_RS25045 0.35 -0.2
5,213,228 - BWI76_RS25045 0.36 -0.1
5,213,228 - BWI76_RS25045 0.36 -2.8
5,213,228 - BWI76_RS25045 0.36 +3.8
5,213,229 - BWI76_RS25045 0.36 +0.4
5,213,233 + BWI76_RS25045 0.36 +1.0
5,213,267 - BWI76_RS25045 0.40 -0.4
5,213,272 - BWI76_RS25045 0.41 -0.5
5,213,275 + BWI76_RS25045 0.41 -1.5
5,213,275 + BWI76_RS25045 0.41 +0.6
5,213,275 + BWI76_RS25045 0.41 -0.4
5,213,275 - BWI76_RS25045 0.41 -0.3
5,213,283 + BWI76_RS25045 0.42 -1.9
5,213,284 - BWI76_RS25045 0.42 -0.0
5,213,288 + BWI76_RS25045 0.42 -0.3
5,213,291 - BWI76_RS25045 0.43 +1.2
5,213,298 + BWI76_RS25045 0.43 +1.0
5,213,306 + +1.6
5,213,306 - BWI76_RS25045 0.44 +0.2
5,213,306 - BWI76_RS25045 0.44 -1.0
5,213,314 - BWI76_RS25045 0.45 -0.6
5,213,314 - BWI76_RS25045 0.45 -0.8
5,213,372 - BWI76_RS25045 0.51 -0.1
5,213,374 + BWI76_RS25045 0.51 +1.0
5,213,459 + BWI76_RS25045 0.60 -0.0
5,213,459 + BWI76_RS25045 0.60 +1.4
5,213,467 - +3.6
5,213,467 - BWI76_RS25045 0.61 -0.3
5,213,467 - BWI76_RS25045 0.61 -0.9
5,213,467 - BWI76_RS25045 0.61 -0.4
5,213,467 - BWI76_RS25045 0.61 -1.8
5,213,503 + BWI76_RS25045 0.65 -0.0
5,213,523 + BWI76_RS25045 0.67 -0.9
5,213,536 + BWI76_RS25045 0.69 -0.8
5,213,548 + BWI76_RS25045 0.70 -2.8
5,213,604 - BWI76_RS25045 0.76 -0.9
5,213,630 + BWI76_RS25045 0.79 -1.3
5,213,638 - +1.6
5,213,675 + BWI76_RS25045 0.83 +0.3
5,213,675 + BWI76_RS25045 0.83 +0.6
5,213,717 + BWI76_RS25045 0.88 -1.0
5,213,774 + +0.6
5,213,777 + -0.9
5,213,777 + +0.0
5,213,777 + +0.3
5,213,782 - -1.1
5,213,785 - -1.0
5,213,810 + -1.8
5,213,942 + -1.2
5,213,950 + -0.8
5,213,950 - -0.1
5,213,958 - +1.5
5,213,974 - -1.9
5,213,979 + -0.2
5,213,984 + +0.7
5,213,984 + -2.2
5,213,987 - +0.4
5,213,987 - -1.8
5,213,992 - -0.6
5,213,992 - -1.6
5,213,992 - +0.6
5,213,992 - +1.1
5,213,992 - -1.7
5,213,997 - -0.1
5,213,997 - +1.2
5,214,002 + -1.8
5,214,004 - +0.4
5,214,004 - +1.0
5,214,010 - -0.8
5,214,010 - -1.0
5,214,033 + +0.4
5,214,040 + +1.5
5,214,058 - BWI76_RS25050 0.10 -0.3
5,214,176 - BWI76_RS25050 0.20 +0.6
5,214,189 - BWI76_RS25050 0.21 -0.7
5,214,280 + BWI76_RS25050 0.28 -1.4
5,214,333 - BWI76_RS25050 0.32 +0.1
5,214,349 - BWI76_RS25050 0.34 -1.3
5,214,423 + BWI76_RS25050 0.40 -0.5
5,214,590 - BWI76_RS25050 0.53 -0.6
5,214,627 + BWI76_RS25050 0.56 -1.4
5,214,784 + BWI76_RS25050 0.69 -1.4
5,214,822 + BWI76_RS25050 0.72 +0.0
5,214,830 + BWI76_RS25050 0.72 -0.8
5,214,895 - BWI76_RS25050 0.78 -0.4
5,214,908 - BWI76_RS25050 0.79 -0.6
5,214,918 - BWI76_RS25050 0.79 +0.1
5,215,093 - -0.7
5,215,128 + -0.9
5,215,497 - -0.0

Or see this region's nucleotide sequence