Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24905 and BWI76_RS24910 are separated by 4 nucleotides BWI76_RS24910 and BWI76_RS24915 are separated by 63 nucleotides BWI76_RS24915 and BWI76_RS24920 overlap by 43 nucleotides BWI76_RS24920 and BWI76_RS24925 are separated by 22 nucleotides BWI76_RS24925 and BWI76_RS24930 are separated by 9 nucleotides
BWI76_RS24905: BWI76_RS24905 - filamentation induced by cAMP protein fic, at 5,189,505 to 5,190,506
_RS24905
BWI76_RS24910: BWI76_RS24910 - rRNA (cytidine-2'-O-)-methyltransferase, at 5,190,511 to 5,191,374
_RS24910
BWI76_RS24915: BWI76_RS24915 - penicillin-binding protein activator, at 5,191,438 to 5,193,519
_RS24915
BWI76_RS24920: BWI76_RS24920 - YraN family protein, at 5,193,477 to 5,193,863
_RS24920
BWI76_RS24925: BWI76_RS24925 - DnaA initiator-associating protein DiaA, at 5,193,886 to 5,194,476
_RS24925
BWI76_RS24930: BWI76_RS24930 - osmotically-inducible protein OsmY, at 5,194,486 to 5,195,061
_RS24930
Position (kb) 5191
5192
5193
5194 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5190.468 kb on + strand at 5190.481 kb on - strand at 5190.508 kb on + strand at 5190.517 kb on - strand at 5190.523 kb on - strand at 5190.536 kb on + strand at 5190.536 kb on + strand at 5190.536 kb on + strand at 5190.544 kb on - strand at 5190.544 kb on - strand at 5190.699 kb on - strand, within BWI76_RS24910 at 5190.911 kb on - strand, within BWI76_RS24910 at 5191.009 kb on + strand, within BWI76_RS24910 at 5191.010 kb on + strand, within BWI76_RS24910 at 5191.125 kb on - strand, within BWI76_RS24910 at 5191.125 kb on - strand, within BWI76_RS24910 at 5191.127 kb on + strand, within BWI76_RS24910 at 5191.135 kb on - strand, within BWI76_RS24910 at 5191.275 kb on + strand, within BWI76_RS24910 at 5191.283 kb on - strand, within BWI76_RS24910 at 5191.319 kb on - strand at 5191.336 kb on - strand at 5191.336 kb on - strand at 5191.721 kb on - strand, within BWI76_RS24915 at 5191.935 kb on - strand, within BWI76_RS24915 at 5191.978 kb on + strand, within BWI76_RS24915 at 5192.049 kb on - strand, within BWI76_RS24915 at 5192.064 kb on - strand, within BWI76_RS24915 at 5192.195 kb on - strand, within BWI76_RS24915 at 5192.334 kb on + strand, within BWI76_RS24915 at 5192.431 kb on + strand, within BWI76_RS24915 at 5192.563 kb on - strand, within BWI76_RS24915 at 5192.681 kb on - strand, within BWI76_RS24915 at 5192.703 kb on + strand, within BWI76_RS24915 at 5192.833 kb on + strand, within BWI76_RS24915 at 5193.028 kb on + strand, within BWI76_RS24915 at 5193.028 kb on + strand, within BWI76_RS24915 at 5193.028 kb on + strand, within BWI76_RS24915 at 5193.036 kb on - strand, within BWI76_RS24915 at 5193.040 kb on + strand, within BWI76_RS24915 at 5193.040 kb on + strand, within BWI76_RS24915 at 5193.041 kb on - strand, within BWI76_RS24915 at 5193.048 kb on - strand, within BWI76_RS24915 at 5193.158 kb on - strand, within BWI76_RS24915 at 5193.170 kb on + strand, within BWI76_RS24915 at 5193.170 kb on + strand, within BWI76_RS24915 at 5193.172 kb on + strand, within BWI76_RS24915 at 5193.207 kb on - strand, within BWI76_RS24915 at 5193.323 kb on + strand at 5193.408 kb on - strand at 5193.412 kb on + strand at 5193.445 kb on + strand at 5193.636 kb on + strand, within BWI76_RS24920 at 5193.745 kb on - strand, within BWI76_RS24920 at 5193.807 kb on + strand, within BWI76_RS24920 at 5194.007 kb on - strand, within BWI76_RS24925 at 5194.186 kb on + strand, within BWI76_RS24925 at 5194.314 kb on - strand, within BWI76_RS24925 at 5194.493 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,190,468 + +0.8 5,190,481 - -1.0 5,190,508 + +0.0 5,190,517 - -0.9 5,190,523 - -0.3 5,190,536 + -2.8 5,190,536 + -2.4 5,190,536 + -2.0 5,190,544 - +0.0 5,190,544 - -0.4 5,190,699 - BWI76_RS24910 0.22 -2.2 5,190,911 - BWI76_RS24910 0.46 -1.9 5,191,009 + BWI76_RS24910 0.58 -2.4 5,191,010 + BWI76_RS24910 0.58 -1.6 5,191,125 - BWI76_RS24910 0.71 -1.3 5,191,125 - BWI76_RS24910 0.71 -2.8 5,191,127 + BWI76_RS24910 0.71 -1.3 5,191,135 - BWI76_RS24910 0.72 +0.5 5,191,275 + BWI76_RS24910 0.88 -1.8 5,191,283 - BWI76_RS24910 0.89 -3.0 5,191,319 - +0.2 5,191,336 - -3.9 5,191,336 - -1.0 5,191,721 - BWI76_RS24915 0.14 -1.5 5,191,935 - BWI76_RS24915 0.24 -3.1 5,191,978 + BWI76_RS24915 0.26 -2.0 5,192,049 - BWI76_RS24915 0.29 +0.1 5,192,064 - BWI76_RS24915 0.30 -1.2 5,192,195 - BWI76_RS24915 0.36 -2.5 5,192,334 + BWI76_RS24915 0.43 -1.5 5,192,431 + BWI76_RS24915 0.48 -1.0 5,192,563 - BWI76_RS24915 0.54 -0.7 5,192,681 - BWI76_RS24915 0.60 -1.4 5,192,703 + BWI76_RS24915 0.61 +0.8 5,192,833 + BWI76_RS24915 0.67 -0.8 5,193,028 + BWI76_RS24915 0.76 -2.4 5,193,028 + BWI76_RS24915 0.76 -0.4 5,193,028 + BWI76_RS24915 0.76 -0.7 5,193,036 - BWI76_RS24915 0.77 -3.6 5,193,040 + BWI76_RS24915 0.77 -0.1 5,193,040 + BWI76_RS24915 0.77 -1.0 5,193,041 - BWI76_RS24915 0.77 -0.3 5,193,048 - BWI76_RS24915 0.77 -0.7 5,193,158 - BWI76_RS24915 0.83 -0.7 5,193,170 + BWI76_RS24915 0.83 -0.6 5,193,170 + BWI76_RS24915 0.83 +0.5 5,193,172 + BWI76_RS24915 0.83 -2.0 5,193,207 - BWI76_RS24915 0.85 -1.5 5,193,323 + -1.3 5,193,408 - -2.0 5,193,412 + -0.1 5,193,445 + -2.3 5,193,636 + BWI76_RS24920 0.41 +0.2 5,193,745 - BWI76_RS24920 0.69 -1.3 5,193,807 + BWI76_RS24920 0.85 +1.0 5,194,007 - BWI76_RS24925 0.20 -1.1 5,194,186 + BWI76_RS24925 0.51 -4.1 5,194,314 - BWI76_RS24925 0.72 +1.5 5,194,493 - +1.0
Or see this region's nucleotide sequence