Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24400 and BWI76_RS24405 are separated by 169 nucleotides BWI76_RS24405 and BWI76_RS24410 are separated by 5 nucleotides BWI76_RS24410 and BWI76_RS24415 are separated by 46 nucleotides
BWI76_RS24400: BWI76_RS24400 - outer membrane channel protein TolC, at 5,085,527 to 5,086,975
_RS24400
BWI76_RS24405: BWI76_RS24405 - hypothetical protein, at 5,087,145 to 5,087,813
_RS24405
BWI76_RS24410: BWI76_RS24410 - putative glutathione-like synthetase, at 5,087,819 to 5,088,979
_RS24410
BWI76_RS24415: BWI76_RS24415 - dioxygenase, at 5,089,026 to 5,089,817
_RS24415
Position (kb)
5087
5088
5089 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 5086.961 kb on - strand at 5087.005 kb on + strand at 5087.037 kb on - strand at 5087.037 kb on - strand at 5087.092 kb on + strand at 5087.095 kb on - strand at 5087.100 kb on - strand at 5087.323 kb on + strand, within BWI76_RS24405 at 5087.343 kb on + strand, within BWI76_RS24405 at 5087.343 kb on + strand, within BWI76_RS24405 at 5087.351 kb on - strand, within BWI76_RS24405 at 5087.405 kb on - strand, within BWI76_RS24405 at 5087.579 kb on - strand, within BWI76_RS24405 at 5087.644 kb on + strand, within BWI76_RS24405 at 5087.649 kb on + strand, within BWI76_RS24405 at 5087.711 kb on - strand, within BWI76_RS24405 at 5087.768 kb on - strand at 5087.973 kb on + strand, within BWI76_RS24410 at 5087.973 kb on + strand, within BWI76_RS24410 at 5088.138 kb on + strand, within BWI76_RS24410 at 5088.405 kb on - strand, within BWI76_RS24410 at 5088.555 kb on - strand, within BWI76_RS24410 at 5088.642 kb on - strand, within BWI76_RS24410 at 5088.728 kb on + strand, within BWI76_RS24410 at 5088.819 kb on + strand, within BWI76_RS24410 at 5088.827 kb on - strand, within BWI76_RS24410 at 5088.837 kb on - strand, within BWI76_RS24410 at 5088.929 kb on - strand at 5088.987 kb on - strand at 5089.231 kb on + strand, within BWI76_RS24415 at 5089.239 kb on - strand, within BWI76_RS24415 at 5089.264 kb on + strand, within BWI76_RS24415 at 5089.310 kb on + strand, within BWI76_RS24415 at 5089.311 kb on - strand, within BWI76_RS24415 at 5089.311 kb on - strand, within BWI76_RS24415 at 5089.319 kb on - strand, within BWI76_RS24415 at 5089.509 kb on - strand, within BWI76_RS24415 at 5089.509 kb on - strand, within BWI76_RS24415 at 5089.571 kb on + strand, within BWI76_RS24415 at 5089.652 kb on + strand, within BWI76_RS24415 at 5089.652 kb on + strand, within BWI76_RS24415 at 5089.652 kb on + strand, within BWI76_RS24415 at 5089.660 kb on - strand, within BWI76_RS24415 at 5089.660 kb on - strand, within BWI76_RS24415 at 5089.660 kb on - strand, within BWI76_RS24415 at 5089.664 kb on + strand, within BWI76_RS24415 at 5089.671 kb on + strand, within BWI76_RS24415 at 5089.672 kb on - strand, within BWI76_RS24415 at 5089.672 kb on - strand, within BWI76_RS24415 at 5089.672 kb on - strand, within BWI76_RS24415 at 5089.696 kb on + strand, within BWI76_RS24415 at 5089.696 kb on + strand, within BWI76_RS24415 at 5089.696 kb on + strand, within BWI76_RS24415 at 5089.704 kb on - strand, within BWI76_RS24415 at 5089.704 kb on - strand, within BWI76_RS24415 at 5089.747 kb on - strand at 5089.779 kb on - strand at 5089.779 kb on - strand at 5089.793 kb on + strand at 5089.827 kb on - strand at 5089.827 kb on - strand at 5089.827 kb on - strand at 5089.839 kb on - strand at 5089.839 kb on - strand at 5089.848 kb on + strand at 5089.856 kb on - strand at 5089.860 kb on - strand at 5089.883 kb on - strand at 5089.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,086,961 - -1.2 5,087,005 + -0.3 5,087,037 - -1.6 5,087,037 - -0.5 5,087,092 + -1.1 5,087,095 - -2.9 5,087,100 - -0.8 5,087,323 + BWI76_RS24405 0.27 -1.4 5,087,343 + BWI76_RS24405 0.30 -4.7 5,087,343 + BWI76_RS24405 0.30 -2.7 5,087,351 - BWI76_RS24405 0.31 -1.3 5,087,405 - BWI76_RS24405 0.39 -0.1 5,087,579 - BWI76_RS24405 0.65 -1.0 5,087,644 + BWI76_RS24405 0.75 -2.9 5,087,649 + BWI76_RS24405 0.75 +2.5 5,087,711 - BWI76_RS24405 0.85 -2.2 5,087,768 - -4.7 5,087,973 + BWI76_RS24410 0.13 -4.8 5,087,973 + BWI76_RS24410 0.13 -4.2 5,088,138 + BWI76_RS24410 0.27 -5.9 5,088,405 - BWI76_RS24410 0.50 -1.9 5,088,555 - BWI76_RS24410 0.63 -2.0 5,088,642 - BWI76_RS24410 0.71 -2.1 5,088,728 + BWI76_RS24410 0.78 -2.3 5,088,819 + BWI76_RS24410 0.86 +0.5 5,088,827 - BWI76_RS24410 0.87 -2.5 5,088,837 - BWI76_RS24410 0.88 -3.4 5,088,929 - -4.0 5,088,987 - -0.2 5,089,231 + BWI76_RS24415 0.26 +1.2 5,089,239 - BWI76_RS24415 0.27 +0.9 5,089,264 + BWI76_RS24415 0.30 -0.6 5,089,310 + BWI76_RS24415 0.36 -1.7 5,089,311 - BWI76_RS24415 0.36 -0.4 5,089,311 - BWI76_RS24415 0.36 -0.1 5,089,319 - BWI76_RS24415 0.37 +0.7 5,089,509 - BWI76_RS24415 0.61 +2.0 5,089,509 - BWI76_RS24415 0.61 +1.5 5,089,571 + BWI76_RS24415 0.69 +0.1 5,089,652 + BWI76_RS24415 0.79 -1.1 5,089,652 + BWI76_RS24415 0.79 +0.4 5,089,652 + BWI76_RS24415 0.79 -0.8 5,089,660 - BWI76_RS24415 0.80 +0.6 5,089,660 - BWI76_RS24415 0.80 +0.5 5,089,660 - BWI76_RS24415 0.80 +2.5 5,089,664 + BWI76_RS24415 0.81 -1.0 5,089,671 + BWI76_RS24415 0.81 -0.2 5,089,672 - BWI76_RS24415 0.82 -1.5 5,089,672 - BWI76_RS24415 0.82 -0.7 5,089,672 - BWI76_RS24415 0.82 -0.6 5,089,696 + BWI76_RS24415 0.85 -0.1 5,089,696 + BWI76_RS24415 0.85 -1.5 5,089,696 + BWI76_RS24415 0.85 -1.9 5,089,704 - BWI76_RS24415 0.86 -0.8 5,089,704 - BWI76_RS24415 0.86 -0.1 5,089,747 - -0.2 5,089,779 - -3.0 5,089,779 - +0.2 5,089,793 + +0.0 5,089,827 - +0.3 5,089,827 - +0.4 5,089,827 - +0.8 5,089,839 - -0.1 5,089,839 - +5.1 5,089,848 + +0.0 5,089,856 - -0.8 5,089,860 - +1.4 5,089,883 - -1.5 5,089,888 - +0.4
Or see this region's nucleotide sequence