Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24375 and BWI76_RS24380 are separated by 30 nucleotides BWI76_RS24380 and BWI76_RS24385 overlap by 1 nucleotides BWI76_RS24385 and BWI76_RS24390 are separated by 24 nucleotides
BWI76_RS24375: BWI76_RS24375 - DNA topoisomerase IV subunit B, at 5,080,927 to 5,082,822
_RS24375
BWI76_RS24380: BWI76_RS24380 - esterase YqiA, at 5,082,853 to 5,083,428
_RS24380
BWI76_RS24385: BWI76_RS24385 - phosphodiesterase, at 5,083,428 to 5,084,255
_RS24385
BWI76_RS24390: BWI76_RS24390 - hypothetical protein, at 5,084,280 to 5,084,702
_RS24390
Position (kb) 5082
5083
5084 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5083.137 kb on + strand, within BWI76_RS24380 at 5083.179 kb on + strand, within BWI76_RS24380 at 5083.187 kb on - strand, within BWI76_RS24380 at 5083.298 kb on + strand, within BWI76_RS24380 at 5083.306 kb on - strand at 5083.340 kb on - strand, within BWI76_RS24380 at 5083.427 kb on - strand at 5083.522 kb on - strand, within BWI76_RS24385 at 5083.556 kb on + strand, within BWI76_RS24385 at 5083.578 kb on - strand, within BWI76_RS24385 at 5083.578 kb on - strand, within BWI76_RS24385 at 5083.578 kb on - strand, within BWI76_RS24385 at 5083.658 kb on + strand, within BWI76_RS24385 at 5083.726 kb on - strand, within BWI76_RS24385 at 5083.778 kb on + strand, within BWI76_RS24385 at 5083.849 kb on - strand at 5083.945 kb on + strand, within BWI76_RS24385 at 5084.023 kb on + strand, within BWI76_RS24385 at 5084.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,083,137 + BWI76_RS24380 0.49 -0.8 5,083,179 + BWI76_RS24380 0.57 -1.5 5,083,187 - BWI76_RS24380 0.58 -2.6 5,083,298 + BWI76_RS24380 0.77 -1.4 5,083,306 - +1.1 5,083,340 - BWI76_RS24380 0.85 -1.5 5,083,427 - +0.0 5,083,522 - BWI76_RS24385 0.11 -2.3 5,083,556 + BWI76_RS24385 0.15 -1.1 5,083,578 - BWI76_RS24385 0.18 -2.1 5,083,578 - BWI76_RS24385 0.18 -3.6 5,083,578 - BWI76_RS24385 0.18 -1.9 5,083,658 + BWI76_RS24385 0.28 -1.5 5,083,726 - BWI76_RS24385 0.36 -2.5 5,083,778 + BWI76_RS24385 0.42 -3.8 5,083,849 - -0.1 5,083,945 + BWI76_RS24385 0.62 -3.3 5,084,023 + BWI76_RS24385 0.72 +0.5 5,084,183 + -2.5
Or see this region's nucleotide sequence