Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21545

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21540 and BWI76_RS21545 are separated by 65 nucleotidesBWI76_RS21545 and BWI76_RS21550 overlap by 36 nucleotidesBWI76_RS21550 and BWI76_RS21555 are separated by 13 nucleotides BWI76_RS21540: BWI76_RS21540 - membrane protein, at 4,510,017 to 4,511,306 _RS21540 BWI76_RS21545: BWI76_RS21545 - nucleotide exchange factor GrpE, at 4,511,372 to 4,511,962 _RS21545 BWI76_RS21550: BWI76_RS21550 - inorganic polyphosphate kinase, at 4,511,927 to 4,512,073 _RS21550 BWI76_RS21555: BWI76_RS21555 - NAD(+) kinase, at 4,512,087 to 4,512,965 _RS21555 Position (kb) 4511 4512Strain fitness (log2 ratio) -1 0 1at 4510.602 kb on + strand, within BWI76_RS21540at 4510.833 kb on - strand, within BWI76_RS21540at 4510.961 kb on + strand, within BWI76_RS21540at 4510.969 kb on - strand, within BWI76_RS21540at 4511.210 kb on + strandat 4511.218 kb on - strandat 4511.232 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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4,510,602 + BWI76_RS21540 0.45 -0.6
4,510,833 - BWI76_RS21540 0.63 -0.5
4,510,961 + BWI76_RS21540 0.73 -0.8
4,510,969 - BWI76_RS21540 0.74 +0.1
4,511,210 + -0.6
4,511,218 - +0.1
4,511,232 + +0.2

Or see this region's nucleotide sequence