Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21110 and BWI76_RS21115 are separated by 208 nucleotides BWI76_RS21115 and BWI76_RS21120 are separated by 32 nucleotides BWI76_RS21120 and BWI76_RS21125 are separated by 96 nucleotides
BWI76_RS21110: BWI76_RS21110 - hypothetical protein, at 4,418,006 to 4,422,952
_RS21110
BWI76_RS21115: BWI76_RS21115 - 3-mercaptopyruvate sulfurtransferase, at 4,423,161 to 4,424,006
_RS21115
BWI76_RS21120: BWI76_RS21120 - enhanced serine sensitivity protein SseB, at 4,424,039 to 4,424,815
_RS21120
BWI76_RS21125: BWI76_RS21125 - aminopeptidase PepB, at 4,424,912 to 4,426,198
_RS21125
Position (kb) 4423
4424
4425 Strain fitness (log2 ratio) -1
0
1
2
3 at 4422.255 kb on + strand, within BWI76_RS21110 at 4422.492 kb on - strand at 4422.517 kb on - strand at 4422.519 kb on + strand at 4422.606 kb on - strand at 4422.653 kb on + strand at 4422.894 kb on + strand at 4423.226 kb on + strand at 4423.482 kb on - strand, within BWI76_RS21115 at 4423.578 kb on - strand, within BWI76_RS21115 at 4423.745 kb on + strand, within BWI76_RS21115 at 4423.980 kb on + strand at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.282 kb on + strand, within BWI76_RS21120 at 4424.290 kb on - strand, within BWI76_RS21120 at 4424.290 kb on - strand at 4424.292 kb on - strand, within BWI76_RS21120 at 4424.384 kb on - strand, within BWI76_RS21120 at 4424.529 kb on + strand, within BWI76_RS21120 at 4424.681 kb on - strand, within BWI76_RS21120
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 4,422,255 + BWI76_RS21110 0.86 -0.2 4,422,492 - +1.0 4,422,517 - +0.2 4,422,519 + +2.0 4,422,606 - -0.4 4,422,653 + +0.4 4,422,894 + +0.1 4,423,226 + -0.2 4,423,482 - BWI76_RS21115 0.38 +0.0 4,423,578 - BWI76_RS21115 0.49 +1.0 4,423,745 + BWI76_RS21115 0.69 +0.9 4,423,980 + +0.7 4,424,282 + BWI76_RS21120 0.31 +3.2 4,424,282 + BWI76_RS21120 0.31 +1.6 4,424,282 + BWI76_RS21120 0.31 +1.7 4,424,290 - BWI76_RS21120 0.32 +3.1 4,424,290 - +3.5 4,424,292 - BWI76_RS21120 0.33 +2.4 4,424,384 - BWI76_RS21120 0.44 +2.8 4,424,529 + BWI76_RS21120 0.63 +2.3 4,424,681 - BWI76_RS21120 0.83 +2.4
Or see this region's nucleotide sequence